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Citations for Promega :
601 - 650 of 968 citations for N BOC 3 FLUORO L TYROSINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Caspase activity was measured with the Caspase-Glo® 3/7 (Promega Corp., Fitchburg, WI, USA) assay kit ...
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bioRxiv - Immunology 2024Quote: ... 3×106 ADCC and ADCP Bioassay Effector Cells expressing the human FcγRIIIa or FcγRIIa receptors (Promega) were added per well and the cells were incubated in at 37°C with 5% CO2 for 6 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Caspase-3 and −7 activity was measured following three days of treatment (Caspase-Glo #G8093, Promega), while cell viability was obtained after six days of treatment (CellTiter-Glo 3D #G9681) ...
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bioRxiv - Microbiology 2023Quote: ... following tryptic digest at 37 °C for 3 h in the same buffer with trypsin (Promega) in a 1:30 ratio (w/w ...
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bioRxiv - Microbiology 2023Quote: ... the cells were co-transfected using Fugene HD transfecting reagent at a 1:3 ratio (Promega) along with pRL-TK Renilla (Renilla Luciferase ...
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bioRxiv - Plant Biology 2023Quote: ... LUC images were taken 2-3 days after infiltration by applying 1 mM Beetle luciferin (Promega) on adaxial leaf surface.
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bioRxiv - Molecular Biology 2023Quote: ... Tryptic digestion was performed for 3 h with 0.1 μg trypsin at 37°C (Promega, V5113) per sample ...
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bioRxiv - Cell Biology 2023Quote: NRK cells were transfected with IP3R-3 siRNA (see below) using Transfast (Promega Corp.; Madison WI) using manufacturer’s instructions ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: Genomic fragment containing the murine Rorc 3’UTR was cloned into psiCHECK2 luciferase reporter plasmid (Promega). This construct was also used to generate the let-7 ʻseed’ deletion mutant derivative using the QuikChange Multi Site Mutagenesis Kit (catalog 200514-5 ...
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bioRxiv - Plant Biology 2024Quote: RNAs including 5’ and 3’ UTRs were synthesized in vitro using T7 RNA polymerase kit (Promega). cDNA from A ...
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bioRxiv - Biochemistry 2020Quote: ... Cellular debris was then pelleted by centrifugation and either 5 μl or 10 μl of the clarified supernatant used in dual luciferase assays following the manufacturer’s prescribed protocol (Promega, cat # E1910). Data are shown as the ratio of RLUC to FLUC activity averaged over six experiments.
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bioRxiv - Biochemistry 2021Quote: ... HCT116 cells were plated on poly-L-lysine coated cover slips and transfected with ER calcium sensor ER-GCaMP6-150 using FuGENE HD (Promega Corp.). Experiments were carried out 1-2 days after transfection ...
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bioRxiv - Evolutionary Biology 2021Quote: ... The 25 µL polymerase chain reaction (PCR) mixture was the same for both mtDNA genes using 5x Green GoTaq® Flexi buffer (Promega), 0.2 mM dNTPs ...
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bioRxiv - Cell Biology 2021Quote: ... 2 μl) were mixed with the master mixes containing 1 μl primers (10 μM each) and reagents of GoTaq G2 (Promega #M7845) and the final volume was 25 μl ...
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bioRxiv - Biochemistry 2024Quote: ... when the temperature was dropped to 22°C and isopropyl β-d-1-thiogalactopyranoside (IPTG) and L-rhamnose (Promega, Madison, WI) was added at 0.2 mM and 0.1% (w/v ...
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bioRxiv - Synthetic Biology 2023Quote: ... Crude extract (10 μl) was mixed with 40 μl of LARII and FLuc activity was determined using a GloMax®-Multi Detection System (Promega) with a 2 s delay and a 10 s integration times ...
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bioRxiv - Molecular Biology 2023Quote: Predigestion of bound proteins was performed by addition of 200 ng / µl of a 1:1 LysC (FUJIFILM Wako Pure Chemical Corporation, 125-02543) trypsin (Trypsin Gold, Mass Spec Grade, Promega, V5280) mix ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was then resuspended in 30 μL of 8 M Urea and 30 μL of ProteaseMax surfactant (20 μg/mL in 100 mM ammonium bicarbonate, Promega, V2071).
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bioRxiv - Cancer Biology 2019Quote: ... Cell viability was measured after 3 days using the CellTiter-Glo 2.0 Cell Viability Assay (Promega, G9243). Luminescence was measured for 500ms using a SpectraMax M3 Microplate Reader (Molecular Devices) ...
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bioRxiv - Synthetic Biology 2019Quote: ... ligated for 3 h at room temperature (RT) or overnight at 4°C using T4 ligase (Promega), and propagated in E.coli XL 10 Gold cells (Agilent) ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... The pellets from centrifugation at 1,500 g for 3 min were lysed in Reporter lysis buffer (Promega) containing 0.16 U µl−1 Ribosafe RNase inhibitors ...
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bioRxiv - Bioengineering 2020Quote: ... Homozygous lpat2-3 T-DNA insertional mutants were identified by PCR using Promega PCR Master Mix (Promega) and combinations of the gene specific and T-DNA left border primers pairs ...
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bioRxiv - Cancer Biology 2020Quote: ... The 3’UTR fragments were cloned in the XbaI site of the pRL-CMV vector (Promega, #E2261) (pCMV-Luc-PTCH1 3’UTR wt/mut) ...
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bioRxiv - Plant Biology 2020Quote: ... using 3 - 5 μg of total RNA and 250 ng of a mixture of random hexanucleotides (Promega) and incubated for 50 min at 50°C ...
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bioRxiv - Cancer Biology 2020Quote: ... The experiment was continued for 3 days and then the cells were treated with CellTiter-Blue (Promega) and incubated for 1 hour ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then incubated for 3 days and viability was assessed using CellTiter- Glo assay (Promega, G7572) following manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: ... The cell pellet (on ice) was resuspended in 3 ml Red Blood Cell Lysis Solution (Promega, Z3141) for 4 min and span down for 5 min at 300g in a pre-cooled centrifuge.
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bioRxiv - Microbiology 2020Quote: ... membranes were incubated in a 0.165 mg/ml BCIP (5-bromo-4-chloro-3-indolyl phosphate, Promega)/ 0.33 mg/ml NBT (p-nitroblue tetrazolium chloride ...
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bioRxiv - Cancer Biology 2021Quote: ... samples were adjusted to 3 mM EDTA and digested with 0.5 μg Trypsin/LysC mix (Promega #V5073) for 1h at 37°C ...
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bioRxiv - Immunology 2021Quote: ... and then lung lobes were inflated with 3% (w/v) 45°C prewarmed low-melting agarose (Promega). The inflated lung lobes were immediately removed and cooled on ice for 15 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were subjected to Caspase 3/7 activity measurement with Caspase-Glo assay kit (Promega, Madison USA). Briefly ...
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bioRxiv - Neuroscience 2022Quote: ... The 3’UTR-Nogo-A-wt product was subcloned into both the pGEM-T-easy plasmid (Promega) and the pBKS plasmid (pBluescript ...
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bioRxiv - Molecular Biology 2022Quote: ... the HIV 5’ LTR from the pNL4-3 isolate (Genbank AF324493) was cloned into pGL3-Basic (Promega) via Gibson assembly (NEB 2X HiFi ...
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bioRxiv - Immunology 2019Quote: Cell viability and caspase 3/7 activity were measured using Non-Radioactive Cell Proliferation Assay kit (Promega) and Caspase-Glo® 3/7 assay kit (Promega) ...
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bioRxiv - Genomics 2021Quote: ... cells were transfected in a 3:1 ratio of FuGENE® HD Transfection Reagent (Promega cat # E2311) and plasmid DNA (pcDNA3.1-HMGB1P1-3xHA ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclear pellets obtained after centrifugation at 1,500g for 3 min were lysed in Reporter lysis buffer (Promega), passed through a 21Gneedle and incubated on ice for 10 min before centrifugation at 17,000g ...
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bioRxiv - Genetics 2020Quote: ... for 3 hours at 37°C in the presence of RNasin Ribonuclease Inhibitor (Promega #N2111, Madison, WI), and PE2 mRNA was purified with MEGAclear™ Transcription Clean-Up kit (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: Genomic DNA extracted from 3 ml fresh EDTA-blood (wizardR Genomic DNA purification Kit, Promega, Madison, WI) was used for genotyping ...
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bioRxiv - Developmental Biology 2019Quote: ... plus the full-length 3□UTR of KIF18A in the psiCheck2 dual luciferase vector system (Promega, Germany) in 10:1 (plasmid DNA:luciferase vector ...
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bioRxiv - Cell Biology 2020Quote: ... samples were incubated for 6 h at 37°C with 3 μg Sequencing Grade Modified Trypsin (Promega). Digestion was terminated via addition of 20 μL trifluoroacetic acid (TFA) ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were alkylated and digested with endopeptidase Lys-C (Wako) for 3 hours and trypsin (Promega) on beads overnight at 37°C.
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bioRxiv - Cancer Biology 2021Quote: ... cell viability and Caspase activity were detected with CellTiter-Glo 2.0 and CaspaseGlo-3/7 assay (Promega) respectively 2 days after transfection ...
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bioRxiv - Developmental Biology 2021Quote: ... the full length of wnt4 including 3’-UTR was cloned into pGEM-T easy vector (Promega, A1360) by primers 5’-ATGTCATCGGAGTATTTGATAAGG-3’ and 5’-AGTCTTTGACACAGCATATATTTC-3’ from cDNA ...
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Synthetic lethal targeting of TET2-mutant hematopoietic stem and progenitor cells by XPO1 inhibitorsbioRxiv - Cancer Biology 2022Quote: ... Relative cell growth at day 3 was evaluated by CellTiter-Glo Luminescent Cell Viability Assay (Promega, #G7571). The concentration of inhibitor required for 50% inhibition of cell viability (IC50 ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were adjusted to 3 mM EDTA and digested with 1.0 μg Trypsin/LysC mix (Promega #V5073) for 1 h at 37 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were adjusted to 3 mM EDTA and digested with 0.5 μg Trypsin/LysC mix (Promega #V5073) for 1h at 37°C ...
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bioRxiv - Systems Biology 2024Quote: ... Cells were first transfected with 3′UTR luciferase constructs (10 ng) using FuGENE HD transfection reagent (Promega) for 4 hours then transfected with Dharmacon miR-199a-5p mimic (50 nM ...
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bioRxiv - Immunology 2023Quote: ... VP1-3 proteins were then bound to the S-Trap column and digested with trypsin (Promega Corporation) for 2 hrs at 47°C ...
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bioRxiv - Cell Biology 2023Quote: ... and 500 ng of pMCB306 plasmids described above along with 3 µl of Fugene 6 (E2692, Promega) transfection reagent ...
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bioRxiv - Immunology 2023Quote: Proteasome activity was measured in cell lysates using the Proteasome-Glo™ 3 Substrate System (Promega, G8531). Corresponding reagents for testing as chymotrypsin-like ...