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Citations for Promega :
601 - 650 of 1130 citations for Crimean Congo Haemorrhagic Fever virus CCHFV glycoprotein C Gc sheep Fc tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Each fraction was digested for 15–18h at 37°C using ultra-grade Trypsin (Promega) at a 1:100 enzyme ...
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bioRxiv - Genomics 2023Quote: ... at 42°C for 8 hours in the presence of RNasin Ribonuclease Inhibitor (Promega N2111) and Pyrophosphatase (NEB M0361S) ...
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bioRxiv - Cancer Biology 2023Quote: ... we added 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA) to the beads soaked in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were precipitated with trichloroacetic acid and digested with Lys-C (Wako) and trypsin (Promega). The peptides were then analysed using an Ultimate 3000 nano-RSLC (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Trypsin digestion was performed at 37 °C with 0.66 µg Trypsin Platinum (Promega, Madison, WI) overnight ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were digested overnight at 37° C with 1 μg mass spectrometry grade Trypsin (Promega). The resulting peptide sample was acidified with 10% formic acid and 10% trifluoroacetic acid (TFA ...
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bioRxiv - Biochemistry 2023Quote: ... followed by 500 ng of trypsin/Lys-C Mix (CAT# V5072, Promega, Madison, WI, USA) and mixed gently at 37°C overnight to digest proteins ...
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Arabidopsis actin-binding protein WLIM2A links PAMP-triggered immunity and cytoskeletal organizationbioRxiv - Plant Biology 2023Quote: ... Proteins were then digested overnight at 37°C with trypsin (Porcine trypsin, Promega, Fitchburg, WI). After stopping the digestion with 1% formic acid ...
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bioRxiv - Plant Biology 2024Quote: ... 4 hours incubation at 37 °C) and 5 μg of trypsin (Trypsin Gold from Promega, in 50 mM TEAB pH 8.5 ...
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bioRxiv - Cancer Biology 2023Quote: ... for 1 hour at 37 °C then with Trypsin (ratio 1:33 enzyme: lysate, Promega) overnight at 37 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The BrdU/C labelled DNA strand was digested with 10 U/ml Exonuclease III (Promega M1811) for two rounds of 10 min at RT ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were digested with Lys-C (Wako Chemicals) for 6 hours and trypsin (Trypsin Gold, Promega) overnight.
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bioRxiv - Molecular Biology 2020Quote: Samples were then incubated overnight at 37°C with 12.5 ng/μL trypsin (sequencing grade; Promega) in 25mM NH4HCO3 ...
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bioRxiv - Molecular Biology 2021Quote: ... thiopropyl sepharose beads were incubated overnight at 37°C with 2 μg trypsin Gold (Promega, #V5280) in 50 mM NH4HCO3 ...
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bioRxiv - Immunology 2022Quote: ... and proteins were subjected to overnight digestion with mass spec grade Trypsin/Lys-C mix (Promega). Following digestion ...
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bioRxiv - Biochemistry 2019Quote: ... the sample was digested overnight at 37 °C with trypsin (1:200 w:w; Promega, Madison, WI). Peptides were desalted using a Sep-Pak (Waters ...
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bioRxiv - Microbiology 2019Quote: ... proteins were incubated overnight at 37 °C with 500bng trypsin (Trypsin Gold Mass Spectrometry Grade, Promega). Peptides were recovered by centrifugation.
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bioRxiv - Systems Biology 2019Quote: ... loaded onto a polyacrylamide gel and digested overnight at 37°C with trypsin (Promega, Wisconsin, USA) at an enzyme-to-protein ratio of 1:100.
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bioRxiv - Biochemistry 2020Quote: ... Reactions were incubated at 37 °C overnight and subsequently treated with 5 units of DNase (Promega) for 1 hour ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were digested overnight at 37°C with 1 μg trypsin (sequencing grade; #V5111, Promega) per 100 μg of protein.
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bioRxiv - Plant Biology 2020Quote: ... were digested for 12 hr at 37 °C with 2 mg of sequencing-grade trypsin (Promega), followed by a second incubation for 6 hr with an additional 2 mg of trypsin ...
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bioRxiv - Plant Biology 2022Quote: The Dynabeads-Co-IP complexes were digested overnight at 37°C in 400ng of trypsin (Promega) per sample in 50 mM NH4CO3 buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and digestion was allowed to proceed overnight at 37°C using trypsin (Promega, Madison, WI, USA) and LysC (Wako ...
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bioRxiv - Biochemistry 2021Quote: ... and proteins were subjected to overnight digestion with Mass Spec Grade Trypsin/Lys-C mix (Promega). The beads were then pulled down and the solutions containing the digested peptides were desalted using AssayMap C18 cartridges mounted on an AssayMap Bravo liquid handling system (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... 150 μg of protein from each sample digested overnight/37 °C with 1.5 μg trypsin (Promega), followed by addition of further 1.5 μg of trypsin and digestion for an additional 4-hrs ...
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bioRxiv - Cell Biology 2021Quote: ... for 4 h at 800 rpm and 42°C or thermolysine (1:50) (Promega, Walldorf, Germany) for 2 h at 800 rpm and 60°C ...
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bioRxiv - Pathology 2019Quote: ... Trypsin digestion occurred in 50 mM ammonium bicarbonate buffer at 37°C for 12 hours (Promega, Trypsin Gold ...
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bioRxiv - Physiology 2019Quote: ... USA) and digestion with 150 ng trypsin (5 h, 37 °C Promega, Sequencing Grade, Wisconsin, USA) using a Tecan Freedom EVO robotic liquid handler (Tecan Systems ...
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bioRxiv - Plant Biology 2020Quote: ... and digested overnight at 37°C with 0.5 µg of sequencing-grade modified porcine trypsin (Promega). The resulting peptides were lyophilized to a final volume of ∼250 µl ...
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bioRxiv - Microbiology 2019Quote: ... The DNase reaction was stopped with RQ1 Stop Solution for 10 minutes at 65°C (Promega). qRT-PCR was performed on 5 ng of total RNA using TaqMan mature miRNA assays and an RNU48 control (Life Technologies) ...
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bioRxiv - Molecular Biology 2019Quote: ... proteins were digested for 6 h at 37°C with 4ng.μL−1 of modified trypsin (Promega) dissolved in 50 mM NH4CO3 ...
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bioRxiv - Neuroscience 2019Quote: ... Proteins were digested overnight at 37°C with 1 μg of mass spectrometry grade Trypsin (Promega). The resulting peptide samples were cleaned up for mass spectrometry in a Sep-Pak C18 column (Waters ...
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bioRxiv - Microbiology 2021Quote: ... and 25 μg of each rNA sample was digested at 37°C overnight with trypsin (Promega). After 10 min at 95°C to denature the trypsin ...
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bioRxiv - Immunology 2021Quote: ... Infection was measured after 5 days at 37°C by the Luciferase assay system (Promega, USA) according to manufacturer’s instructions.
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bioRxiv - Biochemistry 2022Quote: ... aliquots of EPO proteins were digested respectively using a combination of trypsin and Glu-C (Promega), or Glu-C (Promega) ...
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bioRxiv - Biochemistry 2022Quote: ... aliquots of EPO proteins were digested respectively using a combination of trypsin and Glu-C (Promega), or Glu-C (Promega) ...
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bioRxiv - Genetics 2022Quote: ... The lysate was digested overnight at 37 °C with 2 μg of sequencing-grade trypsin (Promega), after which the sample was acidified to 0.1% formic acid ...
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bioRxiv - Plant Biology 2023Quote: ... proteins were alkylated with iodoacetamide and digested with Trypsin/Lys-C Mix (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... In-gel proteolytic digestion was performed overnight at 37°C using either sequencing-grade trypsin (Promega) or EndoGluC (Roche) ...
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bioRxiv - Microbiology 2023Quote: ... following tryptic digest at 37 °C for 3 h in the same buffer with trypsin (Promega) in a 1:30 ratio (w/w ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were then incubated overnight at 37°C with 100 ng trypsin (sequence grade, Promega). The peptides formed from the digestion were further purified by µC18 ZipTips (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... then digested with 4 μL of a trypsin/Lys-C Mix (0.5 μg/uL) (Promega # V5071) using a two-step in-solution digestion overnight at 37°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Tryptic digestion was performed for 3 h with 0.1 μg trypsin at 37°C (Promega, V5113) per sample ...
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bioRxiv - Molecular Biology 2023Quote: ... and subjected to in-gel digestion with trypsin/Lys-C Mix (V5071, Promega, Madison, WI, USA) at 37°C for 12 h ...
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bioRxiv - Immunology 2023Quote: ... Three biological replicates for each condition were digested with Lys-C (Alpha Laboratories) and trypsin (Promega) “on beads” as previously described [44] ...
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bioRxiv - Microbiology 2023Quote: ... The protein extracts were digested by Lys-C endoproteinase and then by trypsin (Trypsin Gold, Promega). Peptides were vacuum-dried and resuspended in 100 µl of solvent A (0.1% (v/v ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were digested atop the filters overnight at 37°C with mass spectrometry grade trypsin (Promega) at a ratio of 1:50 enzyme:protein ...
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bioRxiv - Systems Biology 2024Quote: ... proteins were digested overnight at 37 °C and 800 rpm using sequencing-grade modified trypsin (Promega) at a protein-enzyme-ratio of 50:1 ...
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bioRxiv - Cell Biology 2024Quote: ... Half of the sample was digested with 10 µg trypsin (Promega, 18 h at 37 °C). DOC was precipitated with 10 µL concentrated formic acid (conc ...
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bioRxiv - Neuroscience 2024Quote: ... In-gel digestion was performed overnight at 37 °C with MS grade endo Trypsin/LysC (Promega). The digested peptides were dried using a speedvac and stored at − 20 °C until further processed ...