Labshake search
Citations for Promega :
601 - 650 of 781 citations for Chocolate Agar with Bacitracin for Haemophilus isolation 15x100mm plate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Plates were incubated at 25 °C for 1 hr and fluorescence was measured using a BioTek Synergy Neo plate reader (Promega). The terbium (Tb ...
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bioRxiv - Immunology 2021Quote: ... the plates were washed with PBS and the cells were lysed with 1× reporter lysis buffer (100 µl per well; Promega). Following a freeze-thaw cycle ...
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bioRxiv - Genomics 2021Quote: Cells were transfected in six-well plates (TPP, Switzerland) with 2μg of pSPCas9-2A-CD19 using FuGENE® HD Transfection Reagent (Promega) or Lipofectamine Stem Transfection Reagent (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated for 15 minutes and allowed to shake briefly before ATP was quantified via luminescence readout on the GloMax Explorer multimode plate reader (Promega).
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bioRxiv - Immunology 2022Quote: 1×105 721.221 or 293T cells were plated in 24 well plates and transfected with 400 ng of pGL3 and 100 ng of pRL-CMV (Promega). Two days later ...
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bioRxiv - Biophysics 2022Quote: ... Plasmids were transfected in cells (passage number < 35) seeded in 96-well plates at ~50 % confluence 2-4 days before the experiment with FuGene6 (Promega) or Lipofectamine 2000/3000 following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... cells transduced with the indicated sgRNAs were seeded in a 96-well plate in 100 µL of media and 50 µl of Cell Titer Glo reagent (Promega) was added to each well and the luminescence read on the SpectraMax M5 plate reader (Molecular Devices).
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bioRxiv - Cancer Biology 2022Quote: ... cells were plated with compounds in 384 well plates and viability was assessed after 72hrs using Cell-Titre Glo (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... supernatant was collected and protein concentration quantified using the 660 nm Protein Assay Reagent (Pierce) supplemented with 50 mM Ionic Detergent Compatible Reagent (Pierce) in a GloMax Multi Detection Plate Reader (Promega). After boiling in 1X Laemmli Sample Buffer (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were lysed and the activities of both Renilla and Firefly luciferases measured on a plate reader using the Dual Luciferase Assay System (Promega). For each cell line quantifications were done in technical triplicates ...
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bioRxiv - Cancer Biology 2022Quote: ... CaspaseGlo assays were performed in white-walled 96-well plates to measure the activity of caspase 9 (cat. G8210) or caspase 8 (cat. G8200) (both from Promega) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... were reverse transfected in a 96 well plate format with a custom siGENOME SMARTPool (Dharmacon) siRNA library as described previously72 using Lipofectamine RNAimax Transfection Reagent (Promega). Positive (siPLK1 ...
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bioRxiv - Molecular Biology 2024Quote: Cell viability assays were conducted in 96 well-plate format using Promega’s RealTime-Glo MT Cell Viability Assay (Promega G9712) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... and luminescence from the activity of luciferase was measured at 48 h post-infection using a Tecan Infinite M200 Pro plate reader after applying Bright-Glo reagent (Promega) to the cells.
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bioRxiv - Immunology 2022Quote: ... were sorted by FACS in 96-well V-bottom plate wells containing 2 μl of lysis buffer (Ultra-pure Water, 10% Triton X-100, RNasinPlus 40U/ul; Promega). After evaporation of the lysis buffer at 95°C for 3 min ...
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bioRxiv - Cancer Biology 2023Quote: ... in 6 well plates or 96 well plates (details in the figure legends) and assayed using crystal violet staining or Celltiter-Glo luminescent viability assay (Promega) according to manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... The plates were incubated at 37°C for ~12 hours before luciferase activity was quantified using the Luciferase Assay System (Promega) using SoftMax Pro v.7.0.2 (Molecular Devices) ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of the lysate were transferred into two Microtiter plates (one for Renilla and the second for Bright Glo (Promega)) ...
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bioRxiv - Biochemistry 2023Quote: Approximately 0.1 million cells/well of a 12-well plate were seeded with HEK293T cell line and transfected after 70% confluency using a Lipofectamine reagent (Promega). For the cycloheximide assay ...
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bioRxiv - Microbiology 2023Quote: ... Quantification of OMV production in 96-well plate format was done by performing Nano-Glo assays using the Nano-Glo Live Cell Assay System (Promega). Briefly ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 40,000 cells per well were seeded into poly-D-lysine-coated 96-well plates (Brand, 781965) and the Halo-ligand was added (1:2,000; Promega, G980A). For each transfection ...
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bioRxiv - Bioengineering 2023Quote: For luciferase expression 20 uL of undiluted sample was loaded onto a black 96 well-plate then 100uL luciferin solution (Promega) added immediately before reading on a luminometer (Wallac) ...
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bioRxiv - Cancer Biology 2023Quote: Cells were plated at a density of 1*10^6 per 10cm plate and transfected on the following day using Fugene HD (Promega, Madison ...
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bioRxiv - Bioengineering 2023Quote: ... The day after the plates were pre-cooled (RT, 15’) supplemented with luciferase substrate (Bright Glo Luciferase Assay System, Promega) incubated (RT ...
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bioRxiv - Immunology 2023Quote: ... The reaction was stopped by KPL TBM Stop Solution (SeraCare, #5150-0021) and plates were read at 450nm on GloMax Explorer Multimode Microplate Reader (Promega).
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bioRxiv - Bioengineering 2023Quote: ... 2000 transfected cells were replated into white 384-well tissue culture plates in the presence or absence of 1 μM HaloTag NanoBRET-618 Ligand (Promega) and incubated overnight at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The fluorescence intensity of each well and empty controls was measured at 520 nm excitation on a Glomax plate reader (Promega). The average blank control values for each plate were subtracted from individual fluorescence intensity values and weekly measurements were normalized to the initial reading.
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bioRxiv - Cell Biology 2022Quote: ... 5×104 cells/well were seeded in 24-well plates and transfected with 10 ng pRL-CMV (Promega, Walldorf, Germany) together with either 250 ng pSuper8xTOPFlash or 250 ng pSuper8xFOPFlash [22] using the FuGENE6® reagent (Promega) ...
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bioRxiv - Cancer Biology 2022Quote: ... for 72 hours and cell proliferation was assayed using the CellTiter 96 Non-Radioactive Cell Proliferation kit and a GloMax Discovery plate reader (Promega). For proteomic mass spectrometry ...
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bioRxiv - Cancer Biology 2023Quote: 500 cells/well were seeded in 96-well flat-bottom plates in 100 ml medium and an equal volume of the CellTiter-Glo Luminescent Assay Reagent (#G7571; Promega) added at the indicated time-points ...
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bioRxiv - Cancer Biology 2023Quote: ... the plates were allowed to equilibrate to room temperature for 30 minutes and 25 μL of Cytotox Glo solution (Promega) was added to the wells using a Wellmate plate filler ...
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bioRxiv - Microbiology 2023Quote: ... 8 × 105 HEK293T cells/well were seeded into 6-well plates and transfected the next day with ViaFect (Promega, E4981), using either 4 μg of plasmid expressing the G protein or 2 μg of plasmid expressing HA together with 2 μg of plasmid expressing NA ...
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bioRxiv - Microbiology 2023Quote: ... 50 μl were transferred into a white flat bottom 96 well plate in triplicates and 50 μl of Promega NanoGlo Luciferase assay reagent (Promega) prepared according to the manufacturer’s instructions was added to the wells ...
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bioRxiv - Cell Biology 2023Quote: ... Amounts of protein binding to M.tb were quantified by colorimetric measurements by absorbance at 450 nm using a GloMax plate reader (Version 3.0; Promega Corporation), and plotted based on absorbance values relative to the baseline (negative control ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells was grown at 12-well plates for 18h and transfected with MCHR1 constructs and the cAMP biosensor GloSensor-22F (Promega) at a ratio of 3:2 ...
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bioRxiv - Cancer Biology 2023Quote: ... The following day 100 μl of each sample was transferred to a white 96-well plate and 100 μl of the the mix of Bright-GloTM Luciferase Assay (Promega) was added to each well ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 μL of the cell lysate was transferred into a white 96-well plate and 30 μL of firefly luciferase substrate (Promega) was added ...
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bioRxiv - Cell Biology 2023Quote: ... cells grown to ∼70% confluence in 6-well plates and co-transfected with a gRNA to TBC1D23 (12) and pmaxGFP using Fugene6 (Promega) according to manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... pH 11 and the fluorescence was measured at 360 nm (ex) / 450 nm (em) using GloMax Explorer plate reader (Promega). The heparan sulfatase activity was confirmed by our recently described 6-O desulfation assay using GlcNS6S-GlcA-Glc6SNS-IdoA2S-GlcNS6S-IdoA2S-GlcNS6S-GlcA-pNP (2S2-6S4 ...
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bioRxiv - Biochemistry 2023Quote: Cells were grown in opaque 96-well white plates and then processed according to the manufacturer’s instructions of the CellTiter-Glo Luminescent Cell Viability Assay kit (Promega # G9241). The standard curve of ATP was determined in parallel for absolute quantitation.
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bioRxiv - Bioengineering 2023Quote: For luciferase expression 20 uL of undiluted sample was loaded onto a black 96 well-plate then 100uL luciferin solution (Promega) added immediately before reading on a luminometer (Wallac) ...
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bioRxiv - Microbiology 2024Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using d-luciferin as a substrate (Promega). The percentage of neutralization was calculated as the ratio of the reduction in luciferase readout in the presence of nanobody normalized to luciferase readout in the absence of nanobody ...
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bioRxiv - Bioengineering 2024Quote: ... 20 µL of undiluted sample was loaded onto a white 96 well-plate and the luminescence was read on a luminometer (Promega) after 100 µL of luciferin solution (Promega ...
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bioRxiv - Immunology 2024Quote: ... The plates were then incubated at 37°C overnight before the quantification of luciferase activity using the Luciferase Assay System (Promega) on SoftMax Pro v7.0.2 (Molecular Devices) ...
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bioRxiv - Molecular Biology 2024Quote: ... In a 96 well plate (white bottom) 20 µL lysate was mixed with 50 µL Luciferase Assay Reagent (#E1501 kit, Promega) (as per the manufacturer’s instructions) ...
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bioRxiv - Genetics 2024Quote: ... HEK293T cells cultured in a 12-well plate were harvested and processed according to the instruction of Dual-Glo Luciferase Assay System (Promega) with minor modification ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... NanoGlo Live Cell Assay System substrate was then added and donor emission (450/8 nm BP filter) and acceptor emission (600 nm LP filter) were immediately measured on a GloMax Discover plate reader (Promega). BRET binding signal was defined as acceptor/donor ratio ...
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bioRxiv - Developmental Biology 2024Quote: ... 20,000 cells were seeded in 12-well plates and transfected with 300ng of pCAG-Mest or pCAG-GFP control plasmid using FuGENE HD transfection reagent (Promega) per manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... and then transferred to 96-well plates (white with a clear flat bottom) containing Nano-Glo Luciferase Assay Reagent (Promega) diluted 1000-fold in PBS at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: 1,000 HCECs or M158 cells were plated per well of 96-well plates and proliferation was measured over time using CellTiter Blue (Promega) and a plate reader ...