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Citations for Promega :
601 - 650 of 4462 citations for 7 Bromoacetyl 6 ethyl 1 2 3 4 tetrahydro 1 1 4 4 tetramethylnaphthalene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... digoxigenin-labeled probes were synthesized from the full-length medaka cDNA of oct-4 (stem cell marker also known as pou5f1; [38]) using pGEM®-T Vector (Promega) linearized plasmid ...
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bioRxiv - Immunology 2022Quote: ... Proteins then were reduced with 10 mM DTT in 100 mM Ambic for 30 min at 40oC and alkylated with 55 mM iodoacetamide in 100 mM Ambic for 20 min at 4°C followed by digestion with 0.3 mg trypsin (sequence grade, Promega, Madison, WI) in 50 mM Ambic for 5 h at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 h later 5 µl/well of NanoBRET™ Nano-GloR Substrate (10 µl/ml in DMEM no phenol red, Promega) was added and 460 nm donor and 618 nm acceptor signals were read within 10 min of substrate addition using CLARIOstar microplate reader (Mandel) ...
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bioRxiv - Microbiology 2023Quote: ABIL emulsion oil was prepared by supplementing mineral oil with 4% ABIL EM 90 (Redox, Australia) and 0.05% Triton X-100 (Promega, United States). 425 μL of ABIL oil was added to the PCR mix ...
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bioRxiv - Microbiology 2023Quote: ... infected target cells were washed with DPBS and the medium was replaced with 40 μl of RPMI 1640 with 4% low IgG serum (Promega # G711A) containing 5 x 104 Jurkat effector cells (Promega # G701A ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was cleared by centrifugation at 15,000 g for 15 min at 4°C and 45 μL of the supernatant were mixed with an equal volume of the NanoLuc Reagent (Promega N1110) prepared according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... followed by three washes with TBS-T and 1 hr incubation in TBS-T + 1% milk + anti-mouse serum (1:10,000) (Promega). All steps were carried out at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Thawed tissue was minced and incubated in 1% PFA (with 1 µL mL−1 RNasin Plus (Promega, Madison, WI)) for 5 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... Caspase activity was determined using Caspase-Glo® 3/7 Assay Systems (Promega, Madison, WI) according to the manufacturer’s specifications ...
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bioRxiv - Developmental Biology 2019Quote: ... The Promega Caspase-Glo® 3/7 Assay Systems (Promega, Madison, WI, Cat No. G8091) was used to quantify apoptotic cell death
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bioRxiv - Microbiology 2021Quote: ... caspase activity was measured using Caspase-Glo 3/7 Assay according to manufacturer’s instructions (Promega). Staurosporine (1μM ...
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bioRxiv - Neuroscience 2020Quote: Caspase activity was measured using a chemiluminescent assay kit (Promega Caspase-Glo® 3/7) in 96-well format using an Analyst HT plate reader (Molecular Devices ...
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bioRxiv - Cell Biology 2021Quote: ... and incubated for 24 hr before adding 40 μl of Caspase-Glo 3/7 (Promega) to each well ...
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bioRxiv - Cancer Biology 2021Quote: ... The remaining cells in the inserts were analyzed with Caspase-Glo 3/7 assay (Promega) to confirm that Jurkat cells incubated with TAM CM did not induce apoptosis compared to the control medium.
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bioRxiv - Cell Biology 2020Quote: ... Caspase activation was measured using the CaspaseGlo 3/7 Assay Kit (Promega, Madison, WI, USA) according to manufacturer’s instructions for the indicated time point presented in the figure legend ...
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bioRxiv - Cell Biology 2022Quote: ... Apoptosis was assessed by commercially available caspase 3/7 assay (Cat # G8090, Promega, Madison, WI) as per the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 1,3 μg of plasmid DNA and 3 μl of FuGene 6 (Promega) were mixed in 100 µl Opti-MEMTM medium before the addition to the dish ...
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bioRxiv - Microbiology 2020Quote: Replication competent HIV-1 and VSV-G pseudotyped HIV-1 GFP vectors were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: HIV-1 and lentiviral particles were produced by transfection of HEK293T cells in T150 flasks using Fugene 6 transfection reagent (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: Lentiviral and retroviral particles were produced by co-transfecting HEK293T cells with viral vectors and packaging plasmids at a mass ratio of 1:0.75:0.3 using FuGENE®6 (Promega, E2691). Virus-containing medium was filtrated (0.45 μm ...
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bioRxiv - Genomics 2022Quote: ... The p-dCas9-KRAB and p-PB-Transposase plasmids were co-transfected into NCCIT cells (ATCC) at 70% confluency using a 6:1 ratio of Fugene HD (Promega) for 48 hours ...
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bioRxiv - Microbiology 2020Quote: ... cells were mock treated or treated with 10 unit/mL of uIFN for 6 hrs and collected in 1× passive lysis buffer (Promega). Lysates were assayed for luciferase activity using the Dual Luciferase Reporter Assay System (Promega ...
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bioRxiv - Cell Biology 2022Quote: Halo-MDC1 deletion mutants were expressed transiently by transfecting ∼ 5 × 105 cells with 1 µg of plasmid DNA using FuGene 6 (Promega). For genome editing ...
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bioRxiv - Biophysics 2021Quote: ... were plated into six-well tissue culture plates containing collagen-coated glass cover slips 12 to 24 h before being transfected with 1 μg of the indicated plasmid DNA using FuGENE 6 transfection reagent (Promega) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: Replication-competent NL4-3YU219 HIV-1 molecular clone was used to transfect HEK 293T cells using the FuGENE 6 Transfection Reagent (Promega). The virus culture supernatant was harvested at 48 hours post transfection and stored at −80°C or −150°C.
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bioRxiv - Biochemistry 2022Quote: ... 1.7 μg of attB-hACE2-mNG2-1-10 plasmid or attB-S-mNG2-11 plasmid were added into 5 μL FuGENE 6 transfection reagent (Promega) and OptiMEM (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... Protein mixtures were diluted in 1:6 ratio (v/v) using ultrapure water prior to digestion using sequencing grade trypsin (Promega) at 37°C for 16 h ...
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bioRxiv - Biochemistry 2023Quote: ... 1.5 µg of the pAC8RedNK vector and 0.5 µg of linearized baculoviral DNA were concurrently transfected into 1×106 Sf9 cells using 6 µl of FuGene HD (Promega) in ESF 921 Insect Cell Culture medium (Expression Systems) ...
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bioRxiv - Cell Biology 2023Quote: ... HEK293 cells in a 6-cm dish were transiently transfected with 1 μg of pGloSensor-22F cAMP plasmid (Promega, USA) and 1 μg of human wild-type or mutated A2AR or A2BR overexpression plasmid using polyethyleneglycol (6 μl ...
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bioRxiv - Cancer Biology 2023Quote: Cells were plated at a density of 1*10^6 per 10cm plate and transfected on the following day using Fugene HD (Promega, Madison ...
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bioRxiv - Genetics 2023Quote: ... Protein mixtures were diluted in 1:6 ratio (v/v) using ultrapure water prior to digestion using sequencing grade trypsin (Promega) at 37 °C for 16 h ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was assayed starting at Day 1 for 6 days according to the CellTiter-Glo 3D kit (Promega G9683). For the antiandrogen response assay ...
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bioRxiv - Biochemistry 2024Quote: ... at a 1:75 enzyme to protein ratio for 6 h at 30 °C and then by trypsin (V5111, Promega) (1:50 enzyme to protein ratio ...
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bioRxiv - Cancer Biology 2022Quote: ... pMD2.G and the lentiviral gRNA plasmid at a 3:1:5 mass ratio using FuGENE HD (Promega) in Opti-MEM (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... A DENV-1 3’UTR specific probe was generated by PCR reaction with GoTaq Polymerase (Promega, Wisconsin, USA) containing DIG DNA-labelling mix (Roche ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were transfected with the Piggybac plasmid plus transposase at a 3:1 ratio using Fugene HD (Promega) and selected with G418 (300 µg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... Elution was conducted in 3 beads volume of proteasome buffer containing 1 μL of TEV protease (Promega, PRV6101) for 1 hr at 37°C.
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bioRxiv - Systems Biology 2024Quote: ... followed by a 3:1 dilution with 100mM ammonium bicarbonate and addition of 2µg sequence-grade trypsin (Promega). Samples were digested at room temperature overnight and acidified with formic acid (final concentration 1%) ...
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bioRxiv - Biophysics 2020Quote: ... 1 mM EGTA, 5% glycerol) and freshly supplemented reagents (1 mM DTT, 1 mM ATP, 1 mM PMSF, protease inhibitor mix (Promega) and 1% Tween 20) ...
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bioRxiv - Plant Biology 2019Quote: ... Samples were mixed in a 1:1 ratio with substrate (Promega; E1531) and luminescence was measured ...
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bioRxiv - Microbiology 2021Quote: ... the samples were diluted 1:100 in 1 × passive lysis buffer (Promega) and 5 µl were transferred into a white Nunc 96-well plate ...
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bioRxiv - Immunology 2023Quote: ... and 1 μL RQ1 DNase (1 U/μL, Promega, cat. no: M6101) and then incubated at 37 °C for 30 minutes ...
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bioRxiv - Genomics 2023Quote: ... Samples were then diluted 1:1 with MilliQ water and trypsin (Promega) added at the same enzyme to protein ratio ...
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bioRxiv - Neuroscience 2020Quote: ... all cells were incubated in the following: mouse anti-βIII tubulin (1:1000; 2 hr; Promega) and goat anti-mouse Alexa 546 (1:1000 ...
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bioRxiv - Systems Biology 2021Quote: ... Both 1° and 2° assays were performed with 2x PCR master mix (Promega Corporation, Wisconsin, USA). The final PCR product from the 2° nested PCR was checked by electrophoresis in a 1.5% agarose gel ...
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bioRxiv - Immunology 2021Quote: ... Purified mAb (2 mg/mL in PBS) was treated with 1 unit of IdeS protease (Promega) per 1 µg of mAb ...
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bioRxiv - Plant Biology 2022Quote: ... for 2 h as primary antibody and peroxidase-conjugated goat anti-rabbit antibody (1:10,000; Promega) for 1 h with 5 times 10 min washes in-between the incubations ...
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bioRxiv - Microbiology 2020Quote: ... 1 U RNasin (Promega) and 1 mM DTT ...
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bioRxiv - Molecular Biology 2021Quote: ... 1:1,000 RNasin (Promega)) using a 2ml Dounce homogeniser ...
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bioRxiv - Neuroscience 2020Quote: ... furimazine (Promega, 1:2000), 20 mM HEPES ...