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Citations for Promega :
5951 - 6000 of 9071 citations for Total Carbohydrate Microplate Assay Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2021Quote: Total RNA from two groups of samples were extracted using an SV total RNA isolation system with a DNase purification step (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... All cell lines tested negative for Mycoplasma and all were authenticated in-house (CRUK-Manchester Institute) by examination of a total of 21 loci across the genome using the Powerplex 21 System (Promega).
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bioRxiv - Cancer Biology 2021Quote: Total RNA was isolated from approximately 5×105 cells using the ReliaPrepTM RNA Cell Miniprep System (Promega, UK, Cat. Z6012) following product guidelines ...
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bioRxiv - Molecular Biology 2021Quote: The RT-PCR was performed using 250 ngs of total or polysomal RNA that were reverse transcribed using 50 U of Superscript II (Promega) in a total volume of 25 μL at 42 °C for 1 h ...
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bioRxiv - Molecular Biology 2021Quote: ... and 1 μg of DNase-treated total RNA was applied for cDNA synthesis using an Improm-II Reverse transcription system (A3802, Promega) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: A total of 100 μg of protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation (FASP ...
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bioRxiv - Immunology 2022Quote: Total RNA was isolated from M1 and M2 cells using the ReliaprepTM RNA Cell Miniprep system (Cat. No. Z6010, Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... A 5 mL sample was then mixed with 10 mL of RNAprotect and processed with the SV Total RNA Isolation System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... One microliter of cDNA (corresponding to 50 ng of total RNA) was amplified in 20 μL of reaction mix containing 1× Go Taq qPCR Master Mix (Promega) and 0.5 μM of each primer described in Supplemental Table S2 ...
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bioRxiv - Microbiology 2023Quote: ... the samples were digested by trypsin with 1/80 (w/w) trypsin/total protein ratio (Sequencing Grade Modified Trypsin, Promega) according to the manufacturer recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... Total RNA was extracted from 400 μL of each fraction (spiked with 0.2 ng exogenous control FFLuc mRNA; Promega # L4561) by adding 600 μL TRIzol (Thermo Fisher # 15596018 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... a total of 18 RNA extractions (3 morphs × 3 tissues × 2 biological replicates) were performed using the SV Total RNA Isolation System (Promega) according to manufacturer’s instructions ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... 500 ng of total RNA was used for cDNA synthesis with 15 mM of random hexamers and MMLV reverse transcriptase (Promega), according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Total RNA was extracted from 400 μL of each fraction (spiked with 0.2 ng exogenous control FFLuc mRNA; Promega # L4561) by adding 600 μL TRIzol (Thermo Fisher # 15596018 ...
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bioRxiv - Immunology 2023Quote: The purification of total RNA and reverse transcription to synthesize cDNA were performed using a ReliaPrep RNA Cell Miniprep System (Promega) and ReverTra Ace qPCR RT Master Mix (TOYOBO ...
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bioRxiv - Cancer Biology 2023Quote: ... First-strand complementary DNA (cDNA) synthesis was performed using 2 μg of total RNA with M-MLV reverse transcriptase (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2023Quote: ... PCR was performed for the splicing analysis with the 3 µl of cDNA in a total volume of 20 µl with GoTaq Polymerase 2X (Promega) and 32 amplification cycles ...
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bioRxiv - Cancer Biology 2023Quote: ... the total RNA was incubated with DNase I to remove the trace amount of DNA (Promega Company, Madison. Wisconsin. USA) for two hours ...
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bioRxiv - Plant Biology 2023Quote: ... 1 μg of total RNA was incubated with RNase-free DNase I (1 unit/μl) following the protocol provided by the manufacturer (Promega), followed by reverse-transcription into first-strand cDNA using ImProm II reverse transcriptase (Promega) ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 500 ng of RNA were reverse-transcribed using M-MLV Reverse Transcriptase and Random Hexamer primers (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... Double transfections were performed using 200 ng total DNA containing 100 ng of Fluc reporter vector and 100 ng of Renilla luciferase (Rluc) vector (pRL-TKl; Promega). After 4 hours of transfection ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated on three 10 cm tissue culture dishes per condition and transfected 24 h before lysis using FuGENE 6 (6-12 μg total plasmid DNA; Promega). Published protocol can be found on Protocols.io (dx.doi.org/10.17504/protocols.io.kxygx3zeog8j/v1).
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bioRxiv - Microbiology 2023Quote: For NanoBIT experiments 12,000 HeLa cells were seeded in 96-well black plates and each well was transfected with 100 ng of total DNA using 1:3 ratio of FUGENE HD (#E2311, Promega). Different amounts of plasmid DNA were used to achieve comparable expression of LgBiT/FLAG-tagged and SmBiT/V5-tagged proteins ...
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bioRxiv - Biochemistry 2023Quote: ... The first-strand cDNA was synthesized from 5 µg of the total RNA with an oligo (dT) primer using the AMV reverse transcriptase (Promega). A polymerase chain reaction (PCR ...
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bioRxiv - Neuroscience 2024Quote: ... then transfected with pRK5-based constructs of iGluR subunits and Neto variants (1 µg total DNA/ ml cells) using ViaFect transfection reagent (Promega). The cells were incubated at 37°C for 3 hours ...
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bioRxiv - Plant Biology 2024Quote: ... in presence of 40 U of RNase OUT (both from Invitrogen, using ∼1 μg of total RNA and 100 ng of a mixture of random hexanucleotides (Promega) and incubated for 50 min at 50 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNA was isolated from the liver and eWAT using the ReliaPrepTM RNA Tissue Miniprep System (Promega, Madison, WI, USA) and the RNeasy Fibrous Tissue Mini Kit (Qiagen ...
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bioRxiv - Biochemistry 2024Quote: ... 1 μg of total RNA was then reverse transcribed by extension of random primers using M-MLV (Promega, Madison,WI). Relative expression levels were determined by real-time PCR (qPCR ...
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bioRxiv - Cancer Biology 2024Quote: ... For cDNA synthesis up to 1 µg of total RNA was reverse transcribed using random hexamer primers and M-MLV Reverse Transcriptase (Promega). Gene expression was measured using the primaQUANT CYBR qPCR mastermix (Steinbrenner Laborsysteme GmbH ...
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bioRxiv - Plant Biology 2024Quote: ... The complementary DNA (cDNA) synthesis was performed with 1 μg of total RNA using ImProm-II™ Reverse Transcriptase (Promega). cDNA was diluted in DNase-free water and used for qRT-PCR with Go Taq® qPCR Master Mix reagent (PROMEGA ...
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bioRxiv - Neuroscience 2024Quote: Quantitation of human C1s (total and active) in cerebrospinal fluid (CSF) samples was achieved through direct digestion using LysC (for active C1s, Promega) or Trypsin (for total C1s ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted from the powdered material using the SV Total RNA Isolation System following the manufacturer’s instructions (Promega, USA). For each sample ...
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bioRxiv - Microbiology 2024Quote: ... Exponentially growing and stationary phase cells yielding a total OD600 of 800 were resuspended in 5 ml 1x FastBreak cell lysis buffer (Promega) for 30 minutes on ice and then sonicated afterwards ...
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bioRxiv - Plant Biology 2024Quote: ... First-strand cDNA was synthesized from half a volume of total RNA (1 μg) treated for 30 min at 37°C with RQ1 RNase-free DNase (Promega) using a SuperScript III First-Strand Synthesis System (Invitrogen) ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was performed on 1 μg total RNA using an oligo (dT)18 primer and MMLV reverse transcriptase (Promega). For qPCR ...
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bioRxiv - Plant Biology 2024Quote: ... 5.5 μg of the same total RNA used for the RNA-seq libraries were first treated with RQ1 RNase-free DNase (Promega) to confirm (via negative controls ...
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bioRxiv - Bioengineering 2024Quote: ... The total amount of extracted DNA was quantified using UV-Vis and then analyzed using the Geneprint 24 system (Promega) for its Short Tandem Repeat profile ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA (1µg) isolated from each treatment group was first reverse transcribed using Reverse Transcription (RT) System (Promega Corp, USA) with AMV reverse transcriptase and oligo (dT)15 for the single-strand cDNA synthesis ...
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bioRxiv - Plant Biology 2020Quote: ... 150 mM NaCl2 or 16% PEG and assayed for luciferase activity using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were lysed 24 hours after transfection and the lysates were subjected to dual-luciferase reporter (DLR) assay (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... firefly and Renilla reniformis luciferase activities from lysed cells were determined using the dual-luciferase reporter assay system in accordance with the manufacturer’s protocol (Promega). Data are expressed as the relative firefly luciferase activities normalized to the activity of the R ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplicons that showed positive cleavage from the T7 endonuclease Ⅰ assay were subcloned into the pGEM-Teasy vector (Promega) through TA cloning ...
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bioRxiv - Cancer Biology 2022Quote: ... Luciferase activity was measured with the Dual-Luciferase reporter assay system and normalized for Renilla activity (Promega, Madison, WI). The results are averages of at least three independent experiments assayed in duplicate ± SEM ...
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bioRxiv - Genetics 2022Quote: ... Firefly and Renilla luciferase activity of cell lysates were sequentially assayed using the Dual-Luciferase Reporter Assay System (Promega) on a GloMax Explorer luminometer (Promega) ...
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bioRxiv - Molecular Biology 2021Quote: ... sphere cells growing in the low attachment plates were quantitated by CellTiter-Glo luminescent cell viability assay (Promega, G7571) using a SpectraMax M5 Multi-Mode Microplate Reader (Molecular Devices) ...
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bioRxiv - Bioengineering 2022Quote: ... The cell viability was investigated through the LDH dependent CytoTox 96 Non-radioactive Cytotoxicity assay (#G1780, Promega, Dübendorf, Switzerland.) and the ATP dependent CellTiter-Glo® 2.0 Cell Viability Assay (#G9241 ...
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bioRxiv - Bioengineering 2022Quote: ... Cell proliferation was quantified with the CellTiter 96® Aqueous Non-Radioactive Cell Proliferation Assay (Promega, Madison, WI, USA) as per manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... The trophocidal activity of compounds were assessed using the CellTiter-Glo 2.0 luminescent cell viability assay (Promega, Madison, WI). In brief ...
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bioRxiv - Immunology 2021Quote: ... The cells were incubated for an additional 6 hours at 37°C before 75μl of Bio-Glo Luciferase Assay Reagent (Promega) was added to each well ...
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bioRxiv - Molecular Biology 2021Quote: ... 11 μL of substrate mix (10 μL of Promega Nano-Glo® Luciferase Assay Buffer, 0.1 μL of Promega NanoLuc® luciferase substrate and 1 μL of 10 mg/mL CHX ...