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Citations for Promega :
551 - 600 of 1790 citations for Recombinant Human CLEC11A Protein T7 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... The (119 to 120) bp dsDNA was transcribed in vitro using T7 RiboMAX™ Express Large Scale RNA Production System (Promega Corporation) in 20 µL volumes ...
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bioRxiv - Neuroscience 2021Quote: ... PCR products from the verified plasmids were used as template to synthetize sense and anti-sense complementary RNA probes using in vitro transcription with T7 or SP6 RNA polymerase (Promega, Madison, Wisconsin). RNA probes were tagged with dinitrophenol-11-UTP (DNP ...
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bioRxiv - Genetics 2020Quote: ... The PCR product was the template for the sgRNA synthesis according to the protocol of the RiboMAX Large Scale RNA Production Systems with T7 RNA polymerase (Promega, Fitchburg, USA). After the kits DNAse digestion ...
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bioRxiv - Immunology 2021Quote: ... was verified by Sanger sequencing and used as template in T7-promoter-based in vitro transcription/translation reactions (Promega, Madison, WI: #L1170) using [S35]-methionine (PerkinELmer ...
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bioRxiv - Genetics 2023Quote: ... the vector was used as a template for in vitro transcription using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Cat#P1320). The synthesized sgRNAs were extracted with phenol (pH 4–5):chloroform:isoamyl alcohol (125:24:1 ...
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bioRxiv - Cancer Biology 2021Quote: ... and human Renilla luciferase (pGL4.74[hRluc/TK], Promega, E6921) were used in the study ...
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bioRxiv - Microbiology 2020Quote: ... insert (NRP1, native human ORF in pF1K, FXC23812, Promega): 5’-atagggcgaattcggtagtaaggagcgatcgc-3’ (fwd ...
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bioRxiv - Cancer Biology 2022Quote: ... were amplified by PCR from human genomic DNA (Promega), using the primers listed in Table S1 and then inserted into the psiCHECK-2 vector ...
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bioRxiv - Biochemistry 2022Quote: ... human GR and MR were cloned into pACT (Promega) as N-terminal VP16 fusions using HiFi assembly (NEB) ...
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bioRxiv - Cell Biology 2019Quote: ... A cDNA encoding human KIF1A was purchased from Promega Japan (Promega Japan ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Human PAR2 with N-terminal nano-luciferase (nLuc, Promega) and C-terminal enhanced Yellow Fluorescent Protein (eYFP ...
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bioRxiv - Molecular Biology 2021Quote: ... or goat anti-Human IgG (H+L) (Promega, W4031) antibodies were added for 30 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: 1 ml of recombinant His-MBP-AlpA and His-MBP at a concentration of 10 μM was incubated with 100 μl MagneHis™ Ni Particles (Promega) equilibrated with pulldown-buffer (50 mM Tris pH 7.5 ...
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bioRxiv - Neuroscience 2023Quote: Protein-protein interaction was analyzed using the NanoBit Protein:Protein Interaction (PPI) System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... and clones of the recombinant vector was obtained using Wizard® Plus SV Minipreps DNA Purification kit (Promega). The pACD4K-C vector contains a T7 promoter and ...
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bioRxiv - Microbiology 2022Quote: ... The reverse transcription (RT) mix contained 200 units of recombinant Moloney Murine Leukemia Virus (MMLV) reverse transcriptase (Promega), 20 units of RNAsin (Promega) ...
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bioRxiv - Molecular Biology 2022Quote: ... was mixed with the supernatant (total protein amount of 20-30 μg) supplemented with 6.4 mM MgCl2 and 0.5 U/μl Recombinant RNase Inhibitor (Promega) in total volume of 30 μl ...
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bioRxiv - Developmental Biology 2023Quote: ... modified hypotonic lysis buffer with a 10-fold higher concentration of Cycloheximide and Recombinant RNasin Ribonuclease Inhibitor (Promega) was used instead of RNase OUT ...
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bioRxiv - Biophysics 2021Quote: ... The 2725-nt RNA molecules were synthesized by polymerase chain reaction (PCR) amplification of the plasmids followed by in vitro transcription using the T7 RNA polymerase (Promega, Fitchburg, WI, USA). The RNAs contain a 1206-nt upstream sequence ...
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bioRxiv - Developmental Biology 2021Quote: ... Probes are synthetized from a PCR product or a linearized plasmid (see Table 2) with the T7 or SP6 RNA polymerase (Promega, P4074 or P4084) in presence of DIG-UTP nucleotides ...
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bioRxiv - Developmental Biology 2020Quote: ... The purified PCR products were used as templates to synthesize dsRNA using the T7 RiboMAX Express RNAi System (Promega Corporation, Madison WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... In vitro transcription to generate dsRNA for each target gene and the negative control was performed by combining reagents of the T7 RiboMAX™ Express RNAi System (Promega, Fitchburg, WI) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... DNAs were purified using the PureYield Midiprep kit and were linearized before in vitro synthesis of anti-sense RNA probes using T7 or Sp6 polymerase (Promega, #P2077 and #P108G), RNAse inhibitor and dig-labeled rNTPs (Roche ...
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bioRxiv - Microbiology 2022Quote: ... this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). The primers and probe used in this study were as follows ...
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bioRxiv - Immunology 2020Quote: ... were verified by Sanger sequencing and used as DNA templates in the T7 TNT in vitro transcription/translation kit (Promega, Madison, WI; #L1170) using [35S]-methionine (PerkinElmer ...
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bioRxiv - Microbiology 2021Quote: ... pVHV-SARS-CoV-2-SpikeWT and pVHV-SARS-CoV-2-SpikeOpt replicon vector DNA and helper DNA plasmids were linearized with NotI restriction enzyme prior to in vitro transcription using RiboMAX™ Express T7 RNA polymerase and cap analog (Promega, Madison, WI). Importantly ...
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bioRxiv - Microbiology 2019Quote: ... The primer pairs used in dsRNA synthesis are shown in Supplemental Table 1.1 μg PCR product was used as the template for dsRNA synthesis utilizing the T7 Ribomax Express RNAi System (Promega, Madison, WI, USA). The dsRNA was isopropanol-precipitated ...
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bioRxiv - Microbiology 2021Quote: In vitro transcribed (IVT) RNA standards were generated from the aforementioned plasmids using the T7 RiboMAX™ Express Large-Scale RNA Production System (Promega, Madison, WI). The concentration of each IVT RNA standard was measured by Nanodrop and the molecular weight was used to calculate the expected number of molecules per μL ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified with the Nucleospin gel and PCR clean-up kit (Macherey Nagel) and transcribed in vitro using T7 RNA polymerase (RiboMAX system; Promega, Madison, WI, USA). RNA was purified from the sample using the Nucleospin RNA clean-up kit (Macherey Nagel) ...
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bioRxiv - Cell Biology 2022Quote: Digoxin-labeled RNA probes were in vitro transcribed as per manufacturers’ instructions (DIG RNA labeling Mix, Roche; T7 polymerase, Promega; SP6 Polymerase, Promega) and purified by G-50 column (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: An RNA probe was synthesized in vitro from pSPT19 AS1-S using T7 RiboMAX Large Scale RNA Production Systems (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Bioengineering 2023Quote: ... this template was generated from the cDNA of SARS-CoV-2 AI/I-004/2020 using the T7 RiboMAX Express Large Scale RNA Production System (Promega, Madison, WI, USA). The primers and probe used to detect the WK-521 strain were as follows ...
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bioRxiv - Developmental Biology 2023Quote: ... Germany)-labeled antisense riboprobe was synthesized from the plasmid containing the full or partial length open reading frame of the cDNAs using SP6 or T7 polymerase (Promega, Madison, WI, USA) after restriction enzyme digestion (New England Bio Labs ...
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bioRxiv - Developmental Biology 2023Quote: ... DNAs were purified using the PureYield Midiprep kit and were linearized before in vitro synthesis of anti-sense RNA probes using T7 or Sp6 polymerase (Promega, #P2077 and #P108G), RNAse inhibitor and dig-labeled rNTPs (Roche ...
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bioRxiv - Immunology 2024Quote: ... One μg PCR product was used as the specific-template for synthesizing of dsRNA in vitro by using the T7 Ribomax Express RNAi System (Promega, Madison, WI, USA). The concentration of dsRNA was quantified at 280 nm using a NanoDrop 2000 Spectrophotometer (Thermo Fisher Scientific Inc.) ...
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bioRxiv - Molecular Biology 2024Quote: ... as well as pcDNA3.1-eGFP as a control were used as templates for in vitro transcription and translation using the T7 TNT® Coupled Reticulocyte Lysate System (Promega, Mannheim, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The wild-type and mutated GCE variants were expressed in vitro using the TnT Quick T7 Coupled translation rabbit reticulocyte lysate system (Promega, Madison, WI, USA). The reaction products were examined in the dextran-coated charcoal binding assay4,9 with own synthesized [3H]JH III (25.7 Ci/mmol).62 The reticulocyte lysate without DNA input was the background control.
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bioRxiv - Molecular Biology 2019Quote: PERP protein-protein interactions were isolated using the HaloTag Mammalian Pull-Down System (Promega) and standard protocol ...
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bioRxiv - Plant Biology 2023Quote: ... and the total protein contents were quantified using BCA Protein Assay Reagent (Promega Corporation). The supernatants were stored at –80 ℃ until subsequent analysis.
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bioRxiv - Plant Biology 2024Quote: ... Proteins were expressed with TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega) and reactions were incubated for 12h at 25°C with 10µg pIX-HALO expression clone ...
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bioRxiv - Genomics 2022Quote: ... and the LgBiT Protein (Promega) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Magne Protein G beads (Promega) were used to purify the antibodies according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... In vitro translated proteins (Promega SP6-TNT Quick rabbit reticulocyte lysate system ...
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bioRxiv - Cancer Biology 2022Quote: ... A BCA protein assay (Promega) was used to determine protein concentration ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... HiBiT control protein (#N301A, Promega) dilutions were prepared in the same medium and added (10 µl/well) ...
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Pushed to the edge: hundreds of Myosin 10s pack into filopodia and could cause traffic jams on actinbioRxiv - Cell Biology 2023Quote: ... Halo Standard Protein (Promega, G4491) samples were prepared for final masses of 1 ng ...
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bioRxiv - Biochemistry 2023Quote: ... with purified LgBiT Protein (Promega). Briefly ...
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bioRxiv - Biophysics 2023Quote: ... Protein samples were trypsinized (Promega) in 40mM ammonium bicarbonate (FISHER SCIENTIFIC) ...
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bioRxiv - Molecular Biology 2021Quote: The CD19 minigene was amplified from human genomic DNA (Promega) with the primers 5’-catAAGCTTgaccaccgccttcctctctg-3’ and 5’-catGAATTCNNNNNNNNNNNNNNNGGATCCttcccggcatctccccagtc-3’ ...
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bioRxiv - Neuroscience 2020Quote: ... the human CB1R was cloned into the pCI vector (Promega), and eCB2.0 and eCBmut were cloned into the peGFP-C1 vector (Takara) ...