Labshake search
Citations for Promega :
551 - 600 of 3363 citations for Methyl 2 trifluoromethylsulfonyloxy 1 naphthoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... 0.5 µg (HEK293A, HEK293T, HEK293AΔARRB1/B2) or 2 µg (HEK293AΔGs, HEKS293AΔGi) Glo-22F cAMP plasmid DNA (Promega) and 2 µg MOCK DNA (HEK293A ...
-
bioRxiv - Biochemistry 2024Quote: ... Each sample was divided into 2 aliquots and digested with trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega) and AspN (Sequencing Grade ...
-
bioRxiv - Cell Biology 2022Quote: ... P2-2: -212- -826) were cloned through PCR into the pGL4.20 luciferase reporter vector (Promega, Madison, WI) using C57BL/6 genomic DNA as a template ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were in-gel digested by addition of 200 µL of 2 ng/µl trypsin (Promega, V511A) in resuspension buffer (Promega ...
-
bioRxiv - Microbiology 2022Quote: The intron 2 region was amplified from genomic DNA with GoTaq G2 Flexi DNA polymerase (Promega, M7801) in a final volume of 20 μl as follows ...
-
bioRxiv - Cell Biology 2022Quote: ... cells were transfected with 2 ug of pQCXIN ERAAP-dsRed using Fugene HD transfection reagent (Promega, E2311) following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2023Quote: ... and 2 μg of the linearized DNA was transcribed in vitro using T7 RNA polymerase (Promega Corporation). The obtained RNA was then purified using GeneJET kit (Thermo Fisher Scientific) ...
-
bioRxiv - Cancer Biology 2023Quote: ... cells were incubated with 20 μl of 4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide (MTT) (AqueousOne, Promega) at 37°C for 1-2 hours for MTS colorimetric cell viability assay ...
-
bioRxiv - Cancer Biology 2024Quote: ... Urea was diluted to working 2 M concentration with 20 mM HEPES (pH 8.0) and tryptic (Promega) digestion performed at an enzyme to substrate ratio of 1:25 overnight with incubation at 37°C ...
-
bioRxiv - Biochemistry 2024Quote: ... The reactions were incubated for 2 h at 37 °C followed by DNase RQ1 (Promega, Madison, WI) treatment (2 Units ...
-
bioRxiv - Microbiology 2024Quote: ... ATP content was measured using the BacTiter-Glo™ Microbial Cell Viability Assay Kit (Promega, Cat.G8230/2). Briefly ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were digested overnight at 37°C with 2 µg trypsin (Trypsin Gold, Mass spectrometry Grade, Promega). Digestion was stopped by adding 70 µl 1% trifluoroacetic acid (TFA ...
-
bioRxiv - Microbiology 2024Quote: ... Thawed cell pellets were resuspended with 0.5 ml of 2% CTAB Buffer (Promega Corporation, Madison, Wisconsin, USA), transferred to a Lysing Matrix E tube (MP Biomedicals ...
-
bioRxiv - Biophysics 2020Quote: ... 1 mM EGTA, 5% glycerol) and freshly supplemented reagents (1 mM DTT, 1 mM ATP, 1 mM PMSF, protease inhibitor mix (Promega) and 1% Tween 20) ...
-
bioRxiv - Bioengineering 2022Quote: ... To determine the optimum transfection ratio multiple complexes were created (1.5:1, 3:1, 6:1) using ViaFect Transfection reagent (Promega, E4981) and pGL4.51 [luc2/CMV/Neo] plasmid vector (Promega ...
-
bioRxiv - Cell Biology 2024Quote: ... U2OS cells were transfected with 1 μg of pX330 plasmid and 1 μg of the HDR donor plasmid using 1 ug FuGene 6 (Promega). Approximately 2-3 days post-transfection ...
-
bioRxiv - Cancer Biology 2024Quote: ... 10 mM NaCl, 3 mM MgCl2, 1% BSA, 0.1% Tween-20, 1 mM DTT and 1 U/uL RnaseIn, Promega©) was added to the lysed cells ...
-
bioRxiv - Microbiology 2021Quote: ... the samples were diluted 1:100 in 1 × passive lysis buffer (Promega) and 5 µl were transferred into a white Nunc 96-well plate ...
-
bioRxiv - Genomics 2023Quote: ... Samples were then diluted 1:1 with MilliQ water and trypsin (Promega) added at the same enzyme to protein ratio ...
-
bioRxiv - Microbiology 2024Quote: ... 1 ml DMEM containing 1 % (v/v) triton X-100 (Promega, UK) was then added to each well with vigorous pipetting to detach and disrupt cells from the well surface ...
-
bioRxiv - Microbiology 2024Quote: ... 1 µl forward primer and 1 µl reverse primer (Promega corporation, USA), 3 µl extracted DNA and 14 µl nuclease free water and initial denaturation at 95°C for 10 minutes followed by 35 cycles including annealing temperature which varied in different reaction ...
-
bioRxiv - Microbiology 2020Quote: ... 1 U RNasin (Promega) and 1 mM DTT ...
-
bioRxiv - Molecular Biology 2021Quote: ... 1:1,000 RNasin (Promega)) using a 2ml Dounce homogeniser ...
-
bioRxiv - Neuroscience 2020Quote: ... furimazine (Promega, 1:2000), 20 mM HEPES ...
-
bioRxiv - Neuroscience 2020Quote: ... 1× Protease inhibitor (Promega), 0.1 mM DTT (Thermo Fisher)] per 100 mg of tissue with 15 strokes of the loose and 15–20 strokes of the tight pestle ...
-
bioRxiv - Neuroscience 2020Quote: ... 1 µl DTT (Promega), in 20 µl Superscript II buffer (Invitrogen) ...
-
bioRxiv - Biochemistry 2020Quote: ... 1 μg trypsin (Promega) was added to the S-trap for 90 minutes at 47 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... or (1:25,000) (ProMega) were used ...
-
bioRxiv - Neuroscience 2022Quote: ... and 1 % RNasin (Promega), and incubated for 15 min at 4 °C ...
-
bioRxiv - Cancer Biology 2020Quote: ... 1 mM luciferin (Promega), 14.5 mM NaHCO3 (Sigma) ...
-
bioRxiv - Plant Biology 2023Quote: ... 1 x buffer (Promega), 2.5 mM MgCl2 ...
-
bioRxiv - Cell Biology 2024Quote: ... 1:10000 (Promega: W4021) and in 3.5% (w/v ...
-
bioRxiv - Developmental Biology 2022Quote: ... 0.5ml 1% digitonin (Promega), 0.5ml 10% Tween-20 (Sigma-Aldrich) ...
-
bioRxiv - Immunology 2024Quote: ... 0.5μL 1% digitonin (Promega) and 22uL water ...
-
bioRxiv - Cell Biology 2024Quote: ... Trypsin (1 μg; Promega) was added in 60 μL of 40 mM ammonium bicarbonate buffer ...
-
bioRxiv - Cell Biology 2021Quote: ... psPAX2 and pMD2.G at the ratio of 1:1:1 in HEK293T cells using ProFection Mammalian Transfection System (Promega, E1200), medium was changed 16h post transfection and virus containing supernatant was harvested 48h later ...
-
bioRxiv - Biochemistry 2020Quote: ... three washes of 10 min in PBS-T were performed and membranes were incubated 1 hour at RT with the following 1:5000 or 1:2500 horseradish peroxidase conjugated secondary antibodies in 1% milk in PBS-T: anti-mouse (Promega, #W402B), anti-rabbit (Promega ...
-
bioRxiv - Developmental Biology 2023Quote: ... and 1:2000 RealTime-Glo MT Cell Viability Assay Substrate (to visualise Nluc, first diluted 1:1 in DMSO, Promega G9711).
-
bioRxiv - Cancer Biology 2021Quote: ... total RNA (2 µg) was reverse transcribed into cDNA using a cDNA synthesis kit (Promega, Madison, WI, USA). The RT-PCR was assessed using r-Taq plus Master Mix (Elpis Biotech ...
-
bioRxiv - Cancer Biology 2021Quote: ... Dual luciferase assay was performed 2 days post transfection using the Nano-Glo Dual-Luciferase Reporter Assay (Promega) in a Synergy Neo2 microplate reader (Biotek) ...
-
bioRxiv - Molecular Biology 2021Quote: Cells were transfected with CRISPR–Cas9 and donor plasmids (Table 2) using FuGENE HD Transfection Reagent (Promega, #E2311) in a 6-well plate following the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... PCR fragments with lengths is above 500 bp to 2 kb were carried out with GoTaq enzyme (Promega) according to standard protocols ...
-
bioRxiv - Cell Biology 2021Quote: ... promoter fragments were cloned in front of the Renilla luciferase ORF (hRluc) into the psiCHECK-2 vector (Promega, Cat# C8021 ...
-
bioRxiv - Plant Biology 2021Quote: ... 2 ug of RNA were reverse transcribed using a reverse transcription kit (GoScript™ Reverse Transcription System, Promega). The abundance of specific transcript was measured by probing 1 μL cDNA by quantitative real-time PCR in a total volume of 10 µl containg 5 μL SYBR-green master mix (Roche) ...
-
bioRxiv - Microbiology 2020Quote: ... PCR#2 product was ligated to pGEM-T easy vector or TOPO vector following the manufacturer’s instructions (Promega). After transformation in bacteria and plasmid amplification ...
-
bioRxiv - Microbiology 2022Quote: ... virus was removed and cells were transfected with 2 µg of plasmid DNA pUCSP-A24Rcd using FuGeneHD (Promega) following manufacturer’s recommendations ...
-
bioRxiv - Cell Biology 2022Quote: ... pH 8.0) with 2-5µg plasmids pre-digested by XcmI and 50 µg of herring sperm carrier DNA (Promega), then 700 µL of yeast transformation buffer (40% (w/v ...
-
bioRxiv - Bioengineering 2022Quote: ... Uptake of 2-deoxyglucose by the adipocytes was measured using a Glucose Uptake-Glo™ Assay kit (Promega) and luminescence was measured according to the manufacturer’s instructions using a plate reader ...
-
bioRxiv - Molecular Biology 2020Quote: ... The bacterial surface proteins with attached plasma proteins were released by limited proteolysis with 2 μG trypsin (Promega) /37 °C ...
-
A critical role for heme synthesis and succinate in the regulation of pluripotent states transitionsbioRxiv - Developmental Biology 2022Quote: RNA was extracted after 2 days of culture with the ReliaPrep™ RNA Tissue Miniprep System (Promega, Z6111) following manufacturer’s protocol for non-fibrous tissue by adding RNA lysis buffer on pelleted cells ...