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Citations for Promega :
551 - 600 of 1438 citations for High mobility group protein B1 HMGB1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: Full length 3’-UTR of human (P)RR and LDLR were cloned into the luciferase reporter vector (Promega). Mutant 3’-UTR luciferase reporter vectors were generated by PCR using site-directed mutagenesis technique ...
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bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
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bioRxiv - Neuroscience 2024Quote: ... human CaV1.3 cDNA (accession number NM_001128840.2) was de-novo synthesized and assembled into a HaloTag vector (Promega G7721) using restriction cloning ...
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bioRxiv - Microbiology 2023Quote: ... Standard curves were generated using purified genomic stocks (HSV-1 bacterial artificial chromosome and human genome Promega #G1471). Absolute copy number of genomic stocks was determined using droplet digital PCR (Biorad QX600) ...
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bioRxiv - Microbiology 2021Quote: ... The resulting libraries were checked for their quality using the High-sensitivity DNA chip using the Agilent 2100 Bioanalyzer (Waldbroon, Germany) and quantified using the QuantiFluor One dsDNA kit (Promega). Paired-end (2x300bp ...
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bioRxiv - Bioengineering 2020Quote: ... The drug screens were performed by the Quellos High Throughput Screening Core (University of Washington, Seattle) with CellTiter-Glo (Promega), as well as with CellTox Green (Promega) ...
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bioRxiv - Microbiology 2021Quote: High molecular weight DNA was extracted using the Maxwell® RSC PureFood GMO and Authentication Kit as for qPCR (Promega). The high molecular weight DNA was sent to the Functional Genomic Center Zurich (FGCZ ...
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bioRxiv - Plant Biology 2021Quote: ... benthamiana cDNA using gene-specific primers listed in Supporting Information Table S1 as a NcoI-KpnI fragment by High Fidelity PCR (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2019Quote: ... was amplified via high-fidelity PCR and inserted in-frame along with a firefly luciferase coding sequence (amplified from psiCHECK-2, Promega) with a P2A sequence (Holst et al ...
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bioRxiv - Microbiology 2023Quote: ... Cleaned libraries were then sized-checked with an Agilent Technology 2100 Bioanalyzer using a High sensitivity DNA chip and quantified by Promega Quantus™ fluorometer using OneDNA protocol ...
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bioRxiv - Microbiology 2023Quote: High-molecular weight genomic DNA was isolated based on a modified version of Promega’s Genomic DNA Isolation Kit (Promega, A1120) [63] ...
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bioRxiv - Plant Biology 2023Quote: ... Leaf samples of the high dyad producing plants were stored at -80°C before DNA extraction with the Wizard genomic DNA purification kit (Promega). A pooled DNA sample (equal mass ...
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bioRxiv - Microbiology 2020Quote: PKA activity (PepTag® Non-Radioactive Protein Kinase Assay kit V5340, Promega) was carried out according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Halotag fusion proteins were labelled with Janelia Fluor 646 Halotag Ligand (Promega) for live cell imaging ...
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bioRxiv - Developmental Biology 2021Quote: ... the proteins were in-gel digested with trypsin (Promega, Madison, WI, USA), and the resulting peptide mixtures were processed on StageTips (Rappsilber ...
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bioRxiv - Developmental Biology 2020Quote: ... and 50 μg of protein was digested with sequencing grade trypsin (Promega) at 1:50 trypsin:sample protein amount for 14 hrs on a ThermoMixer at 37°C and 600 rpm ...
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bioRxiv - Cell Biology 2020Quote: ... The resultant protein was then digested overnight with sequencing grade trypsin (Promega). The trypsin ...
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bioRxiv - Plant Biology 2019Quote: ... Chemiluminescent protein detections were conducted with ECL Western Blotting Substrate (Promega, W1001), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Proteins were digested overnight at 37°C with 800 ng trypsin (Promega). Digestion was stopped by adding 5 μl TFA (Trifluoroacetic acid ...
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bioRxiv - Cell Biology 2019Quote: ... Trypsinzation was achieved by incubating eluted protein with excess trypsin (Promega V5111) at 37°C overnight (16-18 hours) ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were then subjected to digestion with trypsin (Promega; Madison, WI, USA) overnight at 37°C at 50:1 protein:trypsin ratio ...
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bioRxiv - Neuroscience 2021Quote: ... the isolated proteins were digested overnight with sequencing grade modified trypsin (Promega). The resulting peptides were resolved on a nano-capillary reverse phase column (PicoFrit column ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were in gel-digested overnight with sequencing grade trypsin (Promega). The resulting tryptic peptides were desalted using in-house C18 disks ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).
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bioRxiv - Neuroscience 2021Quote: ... The binding proteins were eluted by trypsin (Promega Corporation, Madison, WI, USA) for 1 hour at 37°C ...
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bioRxiv - Cell Biology 2021Quote: ... Protein digestion was performed by overnight digestion with trypsin sequencing grade (Promega).
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested with 500 ng of sequencing-grade modified trypsin (Promega), and the resulting peptides were resolved on a nano-capillary reverse phase column ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were digested with 0.2 µg of modified sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate at 37°C overnight ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with 1 μg trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Physiology 2022Quote: ... and proteins therein were in-gel digested with trypsin (Promega, Dübendorf, Switzerland), and resulting peptide mixtures were processed on STAGE tips and analyzed by LC-MS/MS.
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bioRxiv - Biochemistry 2022Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).
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bioRxiv - Plant Biology 2021Quote: ... Total protein was extracted using 100 μL of Passive Lysis Buffer (Promega). 20 μl of the supernatant were used to measure the LUC and RLUC activity using a Dual-Luciferase Reporter Assay System (Promega ...
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bioRxiv - Immunology 2020Quote: ... followed by protein digestion with sequencing grade modified trypsin (Promega, cat. V5111) at a protein-to-enzyme ratio of 50:1 at 37℃ overnight ...
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bioRxiv - Microbiology 2022Quote: ... 300 µg of protein per sample were treated with Trypsin Gold (Promega) overnight ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were digested with trypsin (Trypsin Gold, Promega, Fitchburg, WI, USA) (enzyme-to-protein ratio of 1:50 ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: Eluted proteins were digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA). Each sample was further analyzed by nanoLC-MS/MS on a QExactive+ mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... together with molecular weight marker (Broad range molecular weight protein markers, Promega) was added to a 15% SDS-PAGE gel and separated under 200 volts ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were digested with trypsin (Trypsin Gold, Promega, Fitchburg, WI, USA) (enzyme-to-protein ratio of 1:50 ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein was extracted using a passive lysis buffer (Cat# E1910, Promega). LUC activity was measured using a GloMax®20/20 Luminometer (Cat# E5311 ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were then digested with sequencing-grade trypsin (Promega, Fitchburg, MA, USA) as described previously (Khusainov et al. ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were reduced and alkylated prior to digestion with recombinant LysC (Promega) and trypsin (Promega) ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were reduced and alkylated prior to digestion with recombinant LysC (Promega) and trypsin (Promega) ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were visualized using one-step NBT/BCIP substrates (Promega, Madison, WI).
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Physiological Substrates and Ontogeny-Specific Expression of the Ubiquitin Ligases MARCH1 and MARCH8bioRxiv - Immunology 2021Quote: ... Proteins were reduced and digested with sequence-grade modified Trypsin Gold (Promega). Peptides were eluted with ammonium bicarbonate and acidified peptide mixtures from each biological replicate were analyzed in technical triplicates by nanoflow reverse-phase liquid chromatography tandem mass spectrometry (LC-MS/MS ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested at 37°C for 18 hr with trypsin (Promega) at a concentration of 1:25 (w/w ...
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bioRxiv - Genetics 2019Quote: ... proteins were reduced and alkylated (DTT/Iodoacetamide) before digest with Trypsin (Promega) and desalting of peptides using C18 material (Sola ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were then digested for 3h at RT with 1μg LysC (Promega) and then digested overnight at 37°C with 2μg of Trypsin ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were then subjected to digestion with trypsin (Promega; Madison, WI, USA) overnight at 37°C at 50:1 protein:trypsin ratio ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested over night at 37 °C with trypsin (V5111, Promega) at a 1:20 enzyme to protein mass ratio ...
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bioRxiv - Genomics 2022Quote: ... protein was digested with Trypsin Gold (Catalog No. PRV5280, Promega, Madison, WI) at a 1:50 enzyme-to-substrate ratio for 16 hours at 37 □ ...