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Citations for Promega :
551 - 600 of 655 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... or pCEP4-OCT4-T234A-S235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... at 3,000 cells/well in 50 μL media containing IFN-α for 72 h prior to cell lysis with 50 μL CellTiterGlo 2.0 (Promega, G9243). The reaction was incubated in the dark for 20 min at RT ...
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bioRxiv - Physiology 2024Quote: ... further steps were performed according to the manufacturer’s protocol using proteases trypsin and Lys-C (2 h at 47° C, 1:15 and 1:30, respectively, Promega, USA). Peptides (20 μg ...
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bioRxiv - Microbiology 2024Quote: ... Contaminating DNA in the samples were removed through incubation at 37°C for 2 h using RNase-free DNase I (Promega, USA). All RNAs in the samples were converted into cDNA using a cDNA EcoDry Premix (Clontech ...
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bioRxiv - Developmental Biology 2024Quote: ... The luciferase assay was carried out 48 h after transfection with the Nano-Glo Dual-Luciferase Reporter Assay System (Promega N1610) per manufacturer instructions ...
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bioRxiv - Physiology 2024Quote: ... and hydrolyzed into the S-Trap column using trypsin and Lys-C (2 h at 47 °C, 1:15 and 1:30, respectively, all Promega, USA) in 40 μl 50 mM triethylammonium bicarbonate according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Medium was replaced 12 h after transfection and 48 h after transfection cells were lysed with 50 μL 1x passive lysis buffer diluted in water (Promega, E194A). Cells were frozen at -70°C for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... These near UV-active compounds were instead screened with luminescent assays using the NAD(P)H-Glo™ Detection System (Promega), which monitors the formation of NAD(P)H with the aid of two coupling enzymes ...
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bioRxiv - Cell Biology 2020Quote: ... and 50µl of 0.1mM D-luciferin K+ salt before measuring luminescence in a GloMax® multi microplate reader (Promega).
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bioRxiv - Immunology 2021Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The half maximal inhibitory concentrations (IC50 ...
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bioRxiv - Microbiology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Immunology 2020Quote: ... Luciferase activity was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of infectivity was calculated as ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cell viability was assessed in uninfected PBMC incubated with each PROTAC under the same conditions (1.0 µM, 4 d) using the CellTiter-Blue assay (Promega).
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bioRxiv - Microbiology 2023Quote: ... Imaging was performed 3 min after intraperitoneal (i.p.) injection of 150 mg/kg D-Luciferin (Beetle Luciferin Potassium Salt, Promega) in the IVIS® Spectrum In Vivo Imaging System under 2.5% isoflurane inhalation anesthesia using 15 min exposure ...
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bioRxiv - Cancer Biology 2022Quote: ... Leukaemia propagation was monitored by bioluminescence using an IVIS imaging system (Caliper) following intraperitoneal (i.p.) injection of 150mg/kg D-luciferin (Promega). For LNPs treatment of adult RG mice ...
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bioRxiv - Molecular Biology 2022Quote: ... selected Δku80 and Δku80_Δpks1 strains were plated on Sabouraud and YPD agar paltes supplemented with 0.4 mM of d-luciferin potassium salt (Promega) to confirm the expression of a functional luciferase by bioluminescence imaging using ChemiDoc MP (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of pCS2-HA-NFATc3 was incubated for 2 h at 30°C in 50 μl of the TNT® SP6 coupled wheat germ extract system (Promega, #L5030), according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... secondary antibody staining was performed for 1 h at room temperature using anti-rabbit HRP or anti-mouse HRP secondary antibodies (Promega, 1:10,000) in blocking buffer ...
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bioRxiv - Biochemistry 2021Quote: Pre-treatments were administered as described and glutamate added for 24 h prior to assessing viability using Cell-Titer Glo reagent (Promega; cat. PRG7572) or MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ...
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bioRxiv - Cell Biology 2021Quote: ... and 1-3μl aliquots were taken for reverse transcription (RT) using the Moloney murine leukemia virus RT RNase H (−) (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The antibody-virus inoculum was replaced with fresh culture medium eight hours post-infection and cells processed 48 h post-infection with Nano-Glo luciferase assay system (Promega, Madison, WI). Luminescence was measured using an Enspire plate reader (PerkinElmer ...
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bioRxiv - Biophysics 2020Quote: ... P1KO cells were transiently transfected in a 6-well plate (the cells were seeded 8 h before transfection) in the presence of ruthenium red (10 μM) and mouse Piezo1 (3 μg) using Fugene6 (Promega, Madison, WI). 20–30% of cells showed positive GFP expression indicating successful Piezo1 transfection.
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bioRxiv - Immunology 2022Quote: ... Pseudovirus titers were measured by infecting 293T-ACE2.TMPRSS2s cells for 48 h prior to measuring luciferase activity (luciferase assay reagent, Promega, Madison, WI). For neutralization assays ...
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bioRxiv - Cell Biology 2022Quote: ... The viability of hUC-MSCs after 24 h exposure to increasing concentrations of GNRs was determined by the CellTiter-Glo™ Luminescent Cell Viability Assay (Promega Corporation), which generates luminescent signals based on ATP levels ...
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bioRxiv - Pathology 2021Quote: ... 30 μg of protein was digested with Lys-C (FUJIFILM Wako Chemicals Europe GmbH, Germany) for 4 h and subsequently with modified porcine trypsin (Promega, WI, USA) for 16 h at 37 °C.
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bioRxiv - Microbiology 2020Quote: ... Pseudovirus titers were measured by infecting different cells for 48 h prior to measuring luciferase activity (luciferase assay reagent, Promega, Madison, WI), as described previously (51) ...
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bioRxiv - Immunology 2021Quote: ... H1299 cells were incubated with the pseudovirus for 24 h followed by luciferase assays performed using NanoGlo Luciferase Assay System (Promega, Madison, WI). In one set of experiments ...
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bioRxiv - Molecular Biology 2023Quote: ... Japan) at 37 C for 2 h before digestion overnight with 1 ug Trypsin/Lys-C mix (V5071, Promega, Madison, WI, USA) at 37 C ...
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bioRxiv - Microbiology 2022Quote: ... Luminescence readings were taken at 0 and 72 h post-invasion using the Renilla-Glo® Luciferase Assay System (Promega cat. E2750) and the GloMax® Discover Multimode Microplate Reader (Promega) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The activity of the two luciferase enzymes was measured 24 h after co-transfection following the manufacturer’s instruction of Dual-Luciferase® Reporter Assay System (Cat# E1910, Promega, Wisconsin, USA). Nine biological replications were carried out for each transfection.
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bioRxiv - Neuroscience 2023Quote: ... Membranes were rinsed 5 times for 8 min in PBS-tween 0.2% and incubated with secondary antibodies for 2 h at room temperature under agitation (HRP anti-mouse, 1:10000, Promega cat. # W4021). Membranes were further rinsed 5 times for 8 min in PBS-tween 0.2% ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA from MB cells treated with RA for 48 h was extracted using the SV Total RNA Isolation System kit (Promega, Madison, USA), following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were treated with IFNα (1000 U/mL) for 12 h and were harvested to conduct dual-luciferase reporter assay (Promega, Madison, WI). Firefly luciferase activity values were normalized to Renilla luciferase activity ...
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Development of immortalized rhesus macaque kidney cells supporting infection with a panel of virusesbioRxiv - Microbiology 2022Quote: ... After 72 h medium was removed and cells were lysed in 50 μl Luciferase Cell Culture Lysis Reagent (Promega, Madison, WI, USA). Luciferase activity was meastued in a Plate Chameleon V (Hidex ...
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bioRxiv - Plant Biology 2022Quote: Total RNA of TH3o and O23 was extracted at different stages (24 and 48 h) of barley infection using the SV Total RNA Isolation System (Promega, WI, USA). One microgram of total RNA was used to prepare each sequencing library with the NEBNext Ultra II Directional RNA library prep kit (New England Biolabs Japan ...
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bioRxiv - Microbiology 2024Quote: 100,000 HEK-293T cells were co-transfected with 150 ng GFP/GFP-IpaH4/GFP-IpaH4C339S and 350 ng target vectors for 48 h using FuGENE (Promega; Table S4). Cells were harvested in RIPA buffer containing 100 µM PMSF and protease inhibitor cocktail (Abcam ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were lysed 24 h after transfection and luciferase activity was measured with the Dual-Luciferase Reporter Assay kit (Promega Cat. # E1960) according to the manufacturer’s protocol using a FlUOstar plate reader (BMG Labtech) ...
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bioRxiv - Plant Biology 2024Quote: ... The Halo-DREB2D fusion protein was obtained by coupled in vitro transcription/translation for 2 h at 27 °C using the TNT SP6 High-Yield Wheat Germ Protein Expression System (Promega, WI, USA). Halo-DREB2D synthesis was verified by Western blot using Anti-HaloTag® Monoclonal Antibodies (Promega) ...
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bioRxiv - Microbiology 2024Quote: ... Correct protein size of 41.4 kDa for recEmTDH and 40.3 kDa for recMmTDH was confirmed by western blot using a mouse monoclonal anti-His tag antibody and an anti-mouse IgG (h+l) ap conjugate as secondary antibody (Promega, Dübendorf, Switzerland). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... blots were incubated for 1 h at room temperature with the corresponding horseradish peroxidase-conjugated antibody (1:2000; Promega, Madison, WI, USA) and washed again with TBS-T ...
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bioRxiv - Microbiology 2024Quote: ... Pseudovirus titers were measured by infecting target cells for 48 h prior to measuring luciferase activity (luciferase assay reagent, Promega, Madison, WI), as described previously (58) ...
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bioRxiv - Bioengineering 2024Quote: ... Relative firefly luciferase activity and cell viability were assessed ∼24 h after LNP addition using ONE-Glo™ + Tox Luciferase Reporter and Cell Viability Assay (Promega, Germany).
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was measured 72 h after the drug treatment using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega, Spain). PBS-treated cells were used as control ...
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bioRxiv - Biophysics 2024Quote: The bioluminescent spectra of live HEK293T cells transfected with relevant plasmids were acquired 48 h post-transfection by adding furimazine (Promega, Wisconsin, USA) at a dilution of 1:200 ...
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bioRxiv - Immunology 2021Quote: Cells were washed twice in D-PBS (Wisent) and lysates were collected using the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 and 6 d using a CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (G3582; Promega, Madison, WI). Cells were incubated with the MTS reagent 3-4 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Leukemia burden was assessed using non-invasive bioluminescence imaging by injecting mice intraperitoneally with 75 mg/kg d-Luciferin (Promega), anesthetizing them with 2–3% isoflurane ...
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bioRxiv - Biochemistry 2021Quote: ... and firefly luciferase expression was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). Percentage of infectivity was calculated as the ratio of luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...
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bioRxiv - Microbiology 2022Quote: ... The expression of firefly luciferase was measured on a Berthold Centro LB 960 plate luminometer using D-luciferin as a substrate (Promega). The percentage of neutralization was calculated as the ratio of the reduction in luciferase readout in the presence of mAbs normalized to luciferase readout in the absence of mAb ...