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Citations for Promega :
551 - 600 of 1250 citations for Cytosolic arginine sensor for mTORC1 subunit 2 CASTOR2 Antibody FITC since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... For RNA retrotranscription and RT-PCR it was used GoTaq 2-Step RT qPCR System (Promega) according to the manufacturer’s instructions and it was performed using Mic qPCR Cycler (BioMolecular Systems ...
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bioRxiv - Microbiology 2021Quote: Supernatants were taken from cell medium and mixed with equal volumes of 2×lysis buffer (Promega). Luciferase activity was measured with Renilla luciferase substrate (Promega ...
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bioRxiv - Immunology 2021Quote: ... Purified mAb (2 mg/mL in PBS) was treated with 1 unit of IdeS protease (Promega) per 1 µg of mAb ...
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bioRxiv - Plant Biology 2023Quote: ... 2 μg of each purified plasmid was used as input in a 50 μl TnT (Promega) reaction incubated at 25 °C for 2 h ...
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bioRxiv - Physiology 2023Quote: 2 μg RNA was converted to first–strand cDNA by using reverse transcriptase (Promega, Madison, WI) and Oligo–D (T ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR amplification of exon 2 of the NR2F2 gene was performed using PCR Master Mix (Promega; primer pair is listed in Appendix Table S1) ...
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bioRxiv - Plant Biology 2023Quote: ... cDNA was synthesized (using 2 µg RNA) using the Go Script™ Reverse Transcriptase Kit (Promega) following the manufacturer’s instruction ...
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bioRxiv - Systems Biology 2023Quote: ... and digested overnight at 37°C with a 2 μg Trypsin/Lys-C enzyme mix (Promega). The next day ...
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bioRxiv - Molecular Biology 2023Quote: The pSV-Rluc renilla luciferase internal standard vector was derived from psiCHECK-2 (Promega, catalog # C8021) by digestion with EagI to remove the firefly luciferase gene ...
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bioRxiv - Plant Biology 2023Quote: ... LUC images were taken 2-3 days after infiltration by applying 1 mM Beetle luciferin (Promega) on adaxial leaf surface.
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bioRxiv - Cell Biology 2023Quote: HeLa cells were transfected using 2 µg of plasmid DNA and 6 µL of FuGENE (Promega) for gene editing or using a NEPA21 electroporation system (Nepa Gene ...
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bioRxiv - Biochemistry 2023Quote: ... 0.02 µg of plasmid and 2 µg of carrier DNA were combined with FuGENE HD (Promega) at a 3:1 ratio and added per well of a 6-well plate seeded with 70% confluent HEK293 cells ...
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bioRxiv - Microbiology 2023Quote: ... described in Supplemental Table 2 were used for amplification with GoTaq G2 polymerase (Promega, WI, USA). Amplification was performed by 35 cycles PCR each consisting of 94°C for 30 sec ...
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bioRxiv - Cell Biology 2023Quote: ... cells were incubated in a medium containing 2 nM Janelia Fluor 646 (JF646) HaloTag ligand (Promega) and 0.5 μg/mL Hoechst33342 (Nacalai Tesque ...
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bioRxiv - Biochemistry 2022Quote: ... by quickly mixing 2 μL of above refolding mixture with 50 μL of luciferase substrate (Promega). The activity of unheated luciferase in buffer B was set as 100%.
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bioRxiv - Immunology 2022Quote: ... mixed with Lumit BiT Set 2 (anti-mouse Ab-SmBiT, anti-rabbit Ab-LgBiT, Promega W1220) and added for 90min ...
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bioRxiv - Genetics 2022Quote: ... The lysate was digested overnight at 37 °C with 2 μg of sequencing-grade trypsin (Promega), after which the sample was acidified to 0.1% formic acid ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were successively lysed and assayed by a 2-DG uptake assay kit (J1341, Promega) according to the instruction ...
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bioRxiv - Microbiology 2024Quote: ... and 2 mL of the filtrate was transferred to tubes containing polyethylene glycol (PEG-8000, Promega) to achieve the final PEG concentration of 10% ...
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bioRxiv - Immunology 2024Quote: ... and 75 µl of an MTS/phenazine methyl sulfate (PMS) solution (2 mg/ml MTS (Promega) and 46 μg/ml PMS (Sigma–Aldrich ...
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bioRxiv - Immunology 2024Quote: ... 1 mL of warm (37-40°C) 2% agarose low-melting point (Promega® Cat. V2111) was infused through the trachea into the lungs using a polythene tube with a 1.5 mm diameter (Portex Cat ...
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bioRxiv - Cancer Biology 2024Quote: ... the RNA samples were additionally pre-treated with 2 units of RQ1-DNAse (Promega, cat# M6101) per 1 µg of RNA at 37 °C for 30 minutes ...
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bioRxiv - Plant Biology 2024Quote: ... Aliquots #1 and #2 were then treated with 1 mg/mL trypsin (Promega, Madison, WI, USA) and incubated at 37 °C for 1 h followed by the addition of 1.5 mg/mL trypsin inhibitor (Worthington Biochemical Corp ...
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bioRxiv - Bioengineering 2024Quote: ... 10μM of 2-(4-Morpholinyl)-8-phenyl-4 H-1-benzopyran-4-one (Ly294002: Promega, #V120A), 50ng/ml of Activin A (Stemcell Technologies ...
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bioRxiv - Cell Biology 2024Quote: ... Protein lysates from the single cells were digested with 2 ng of Trypsin (Promega, Cat. V5280), dissolved in 1 μl of 100 mM TEAB pH 8.5 containing Benzonase (Sigma ...
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bioRxiv - Genomics 2024Quote: ... We measured 2-deoxyglucose uptake with the Glucose Uptake-Glo™ Assay (Promega, Fitchburg, WI, USA) following manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... As secondary antibodies we used horseradish peroxidase coupled anti-mouse (Promega W402B), anti-rabbit (Promega ...
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bioRxiv - Microbiology 2020Quote: ... Immune complexes were detected with anti-rabbit peroxidase-conjugated secondary antibodies (Promega) and enhanced chemiluminescence reagents (Pierce ...
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bioRxiv - Developmental Biology 2021Quote: ... The primary antibodies used are mouse β-Gal (1:250; Promega #Z3781), mouse anti-Wingless (1:100 ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 μL of rabbit polyclonal antibody against PLRV-CP (1:5000; Promega) was added to each well and incubated for 3 h at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... Secondary antibodies used in Western blotting were conjugated to alkaline phosphatase (Promega) or horseradish peroxidase.
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bioRxiv - Microbiology 2020Quote: ... Secondary HRP conjugated goat anti-mouse IgG antibodies (Promega, Madison, WI; W4021) was used at 1:50,000.
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bioRxiv - Cell Biology 2022Quote: ... Secondary antibodies conjugated to horseradish peroxidase (1:10,000, W401B and W402B, Promega) were applied in the same solution for one hour at room temperature before activity was detected (SuperSignal West Pico PLUS 34580 ...
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bioRxiv - Molecular Biology 2020Quote: ... and the goat anti-rabbit antibody (anti-rabbit IgG AP conjugate, Promega) as secondary antibody (30) ...
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bioRxiv - Genetics 2021Quote: ... the following antibodies were used: mouse anti-beta-galactosidase (Promega; 1:100), mouse anti-Mmp1 (1:1:1 mixture of DSHB 3A6B4 ...
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bioRxiv - Microbiology 2023Quote: ... Secondary antibodies used in Western blotting were conjugated to alkaline phosphatase (Promega) or horseradish peroxidase.
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bioRxiv - Microbiology 2023Quote: ... followed by horseradish peroxidase-conjugated (HRP) secondary rabbit antibodies (Promega, 1/25000) and revealed by chemiluminescence (Pierce ...
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bioRxiv - Developmental Biology 2024Quote: ... the membrane was incubated in secondary antibody conjugated to horseradish peroxidase (Promega) at a dilution of 1:5000 for 1h at room temperature ...
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bioRxiv - Genetics 2023Quote: ... Secondary horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG antibodies (Promega) were used at 1 ...
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bioRxiv - Neuroscience 2023Quote: ... The immunoblots were probed with appropriate secondary antibodies (Promega and Li-Cor) and visualised by chemiluminescence (Supersignal detection kit ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibodies used were: anti-rabbit-IgG-HRP (W401B, 1:8000, Promega); anti-mouse-IgG-HRP (W402B ...
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bioRxiv - Biochemistry 2022Quote: ... membrane was incubated with anti-HaloTag antibody (1:1000; Promega Corporation; G9281) and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibody signal was detected using the ECL Western Blotting Substrate (W1015, Promega) and X-ray film (F-BX810 ...
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bioRxiv - Molecular Biology 2024Quote: ... secondary antibodies: anti-rabbit HRP or anti-mouse HRP (Promega, 1:10000).
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bioRxiv - Genomics 2024Quote: ... The following secondary antibodies were used: anti-rabbit IgG HRP (Promega, #W4018). Blots were incubated with the primary antibody overnight at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... Bound antigen-antibody complexes were precipitated using streptavidin-coated magnetic beads (Promega) for at least 1 hr at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... proteins were detected with secondary peroxidase-conjugated antibodies (Promega, Madison, WI, USA) and ECL ...
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bioRxiv - Genetics 2020Quote: ... 2 µg of DNA in 50 µl of DMEM and 50 µl of FuGene6 transfection reagent (Promega) at 1mg·ml-1 in DMEM were incubated separately for 5 min at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... the cells were transfected with 1 – 2 μg EphA2-mTurquoise plasmid DNA using FuGene HD (Promega, #E2311) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2021Quote: ... The results were calculated using the 2-△△CT method according to the GoTaq qPCR Master Mix (Promega) manufacturer’s specifications.