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Citations for Promega :
551 - 600 of 4312 citations for Cow Casein Kinase II Subunit Alpha 2 CSNK2A2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... 2 μg of total RNA treated with DNase RQ1 (PROMEGA) was reverse transcribed with Superscript IV (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... 2-3 µg bacmid DNA was transfected (FuGene HD, Promega) into 2 ml Sf9 cells plated at 0.5x106 cells/ml density in a 6-well plate and left to incubate for 5-7 days 27ºC without shaking ...
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bioRxiv - Systems Biology 2024Quote: ... before adding 2 µL of 0.5 ng/µL trypsin (Promega) in digestion buffer (50 mM TEAB ...
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bioRxiv - Cell Biology 2024Quote: ... the protein suspension was digested with 2 µg trypsin (Promega) overnight at 37°C ...
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bioRxiv - Immunology 2022Quote: ... The larger of either 1μg of RNA or 5μL of RNA were used as a template for cDNA synthesis by the ImProm-II reverse transcriptase system (Promega, Madison, WI) after DNA contamination was removed with the DNAfree kit (Life Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured by adding 25 μL Luciferase Assay Buffer II (LARII) to 5 μL of lysate and measured immediately with the GloMax 20/20 Luminometer (Promega, E5311). Reactions were stopped and Renilla luciferase activity was measured by adding 25 μL Stop&Go reagent ...
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bioRxiv - Neuroscience 2021Quote: ... First strand cDNA was synthesized in 20 µl reaction using oligo-dt primers and ImProm-II Reverse Transcriptase (Promega, Milan, Italy) with 5 µl of RNA according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... 3 μg of total RNA was incubated with 1mM of Oligo-AP primer (Supplementary Table I) and Improm II reverse transcriptase (Promega®) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from Hydra polyps starved for 48 hrs and cDNA was synthesized from total RNA using Improm-II reverse transcriptase system (Promega™) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... purified total RNA was reverse transcribed into cDNA by ImProm-II™ Reverse Transcription System (Promega; Madison, WI; Cat. No. A3800). Target gene fragments were first amplified from a mixed cDNA library of planula larva and primary polyps ...
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bioRxiv - Neuroscience 2022Quote: ... First strand of cDNA was synthesized from 0.5-1 μg of total RNA using Im-Prom-II system (Promega, Cat. A3800). Real-Time PCR was performed using iTaq qPCR master mix according to manufacturer’s instructions (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA (1 μg) was reverse-transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, Fitchburg, WI, USA).
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bioRxiv - Plant Biology 2022Quote: ... Total RNA (1 μg) was reverse-transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, Fitchburg, WI, USA).
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bioRxiv - Molecular Biology 2019Quote: ... Two micrograms of total RNA were reverse transcribed into the first strand cDNA using the ImProm-II Reverse Transcription System (A3800, Promega, Madison, USA) according to the manufacturer’s protocol and used as a template for the amplification of the full-length camel IFNε cDNA ...
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bioRxiv - Neuroscience 2021Quote: ... One microgram of total RNA was transcribed to cDNA by reverse transcriptase enzyme action Improm Pre-II ® (Promega, Madison, Wisconsin, USA). Quantitative RT-PCR reaction in real time was done on an ABI Prism ® 7500 Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... DNase-treated RNA was transcribed into complementary DNA (cDNA) using the ImProm-II™ reverse transcription system and oligo-dT as primer (Promega, Germany). Quantitative real-time reverse transcriptase polymerase chain reaction (qPCR ...
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bioRxiv - Neuroscience 2023Quote: ... 1ug of total RNA extracted from transfected SK-N-MC cells was reverse transcribed using the ImProm-II Reverse Transcription System and random hexamer primers (Promega, Madison, MI). PowerUp SYBR™ Green Master Mix (Thermo Fisher ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized with primers specific for each strand separately at 50 °C (Supplemental Table S1) using the ImProm-II™ Reverse Transcription System (Promega, A3800) and subjected to qPCR with primers specific for the viral N-protein (Supplemental Table S1).
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed using 1 µg of total RNA in combination with oligo(dT) and Improm-II reverse transcriptase (Promega, Madison, WI). qPCR analysis was performed as described previously [55] with SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Molecular Biology 2020Quote: ... and 2 µl/well of FuGENE 6 Transfection reagent (Promega; E2691) following the manufacture’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... and 2 µl/well of FuGENE 6 Transfection reagent (Promega; E2691) following the manufacture’s protocol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... or pB.2-eIF2α-S51A plasmids by use of FuGENE6 (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Each reaction was treated with 2 units of RQ1 DNase (Promega) for 30 min at 37°C ...
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bioRxiv - Neuroscience 2021Quote: ... 2 μl of Trypsin/Lys-C mix (0.5μg/μl, Promega, V5073) was added to each sample and incubated for 3 hrs at RT in the dark ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were digested by addition of 2 % (w/w) trypsin (Promega) over night at 37°C after adding 50 mM ammonium hydrogen carbonate to a final concentration of 1 M urea ...
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bioRxiv - Plant Biology 2020Quote: ... from 2 μg RNA treated with RQ1 RNase-free DNase (Promega). The cDNA was then diluted 20 times in water and 5 μl of the dilution was used for the realtime qPCR analysis ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were additionally diluted 2-fold and digested with trypsin (Promega, Sequencing Grade Modified Trypsin ...
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bioRxiv - Cell Biology 2022Quote: ... 2 µg Lys-C/Trypsin mix protease (Promega, Mass spec grade) were added to each sample (10 mM EPPS pH 8.5 ...
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bioRxiv - Cancer Biology 2019Quote: ... The RdRP products were treated with RNase I (2 U, Promega) at 37°C for 2 hours to digest single-stranded RNAs completely ...
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bioRxiv - Microbiology 2019Quote: ... 5 μl of 2× GoTaq q-PCR master mix (Promega, USA) and 0.6 μM of AF77/78 or AF79/80 primer pairs targeting a region close to the Cori or to the terminus (ter) ...
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bioRxiv - Genetics 2020Quote: ... 2 μg RNA were treated with RQ1 RNase-free DNase (Promega) followed by reverse transcription using M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 μg of MS grade Trypsin Platinum (Promega, Madison, WI, USA) in 40 μL of digestion buffer containing 50 mM TEAB was added into the filter and digested at 37°C for 16 h ...
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bioRxiv - Cancer Biology 2022Quote: ... Nano-Glo Luciferase Assay Substrate furimazine (2 µl ml-1, Promega) was added and the nLuc released upon proteolytic cleavage of the receptor was measured on a plate reader Mithras LB 940 (Berthold Technologies ...
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bioRxiv - Physiology 2023Quote: ... The assay medium was supplemented with 2% GloSensor Reagent (Promega #E1291) and 0.1% BSA ...
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bioRxiv - Microbiology 2023Quote: ... 5 μM 11S and 2 μl furimazine solution (Promega Cat. # N1610) were added and the luminescence read at 1 min intervals for 10 min using the FLUOstar Omega using a gain value of 3,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids (2 μg /dish) were transfected using FuGENE6 transfection reagent (Promega), according to the manufacturer’s instructions.
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bioRxiv - Plant Biology 2023Quote: ... Any remaining RNA was removed with 2 µl RNase A (Promega) added to a pool of DNA samples ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 2 μL/well of One-Glo detection reagent (Promega, cat # E6120) was added to all microplate wells via Multidrop ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µl of RNAse A solution (4 mg ml-1) (Promega) were added before incubation at 37°C for 15 min ...
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bioRxiv - Cancer Biology 2023Quote: ... the GoTaq 2-step RT-qPCR system (Promega, Madison, MA, USA) was utilized to perform cDNA synthesis and qPCR ...
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bioRxiv - Physiology 2024Quote: The 2-DG glucose uptake for tissues was analyzed by Promega Glucose uptake-Glo assay method (#J1341) ...
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bioRxiv - Molecular Biology 2024Quote: ... Crosslinks were reversed by addition of 2 uL Proteinase K (Promega) and incubation at 65 °C for 12-16 hours in a Thermomixer ...
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bioRxiv - Molecular Biology 2024Quote: ... 10X Primer extension buffer and 2 U AMV Reverse Transcriptase (Promega) were added to the annealing mixture and incubated for 1 hour at 42 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 ng of Renilla luciferase expressing pRL SV40 plasmid (E2231, Promega), as internal control ...
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bioRxiv - Cell Biology 2020Quote: ... caspase8 Kit (Promega company); Caspase3 / 7 Kit (Promega company).
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bioRxiv - Molecular Biology 2020Quote: ... or Maxwell kit (Promega). Following RNA extraction ...
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bioRxiv - Genetics 2023Quote: ... the GoScript Kit (Promega) was used to make cDNA and qRT-PCR performed on a BioRAD c1000 Touch thermocycler.
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bioRxiv - Microbiology 2020Quote: ... and a total of 300 ng treated-RNA was used for reverse transcription reaction with ImProm-II Reverse transcriptase (Promega, Madison, WA, USA). The RT-qPCR was assessed on a Real-time PCR StepOne Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Molecular Biology 2019Quote: ... and the first-strand cDNA was synthesized from 1 μg of total RNA with oligo dT or random primers using ImProm-II reverse transcriptase (Promega, Madison, WI, USA). The qPCR reactions were performed in a 20 μL reaction mixture using SYBR Green PCR Master Mix (Roche ...
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bioRxiv - Physiology 2020Quote: ... The beads were resuspended in 10 μl of reverse transcriptase mix (100□U, SuperScript II, Invitrogen; 10 U, RNAsin, Promega, Madison, Wisconsin, USA), 1□×□Superscript II First-Strand Buffer ...