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Citations for Promega :
5901 - 5950 of 8213 citations for QuantiChrom Indole Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the amount of pseudoviruses prepared was quantified by the HiBiT assay using a Nano Glo HiBiT lytic detection system (Promega, Cat# N3040) as previously described32,57 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The activity of the two luciferase enzymes was measured 24 h after co-transfection following the manufacturer’s instruction of Dual-Luciferase® Reporter Assay System (Cat# E1910, Promega, Wisconsin, USA). Nine biological replications were carried out for each transfection.
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bioRxiv - Cancer Biology 2023Quote: ... cell viability was determined over time course with a a microplate reader using CellTiter-Glo® 3D Cell Viability Assay (Promega, G9683) according to manufacturer’s directions ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were harvested 24 hours after transfection and luciferases activity was measured using Nano-Glo® Dual-Luciferase® Reporter Assay System (Promega) on Berthold Multimode reader Tristar2S ...
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bioRxiv - Cancer Biology 2022Quote: ... cell viability and caspase 3/7 activity were evaluated using CellTiter-Glo and Caspases-Glo 3/7 assays (both from Promega, Madison, WI), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: Caspase 3/7 activity was quantified in cell culture by using the Caspase-Glo 3/7 assay system (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Developmental Biology 2023Quote: ... LacZ reporter activity was assayed in defined media (SD) supplemented with -Leu-Trp using the Beta-Glow® Assay System following commercially available protocols (Promega #E4720). In short ...
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bioRxiv - Biochemistry 2023Quote: ... cells expressing dual luciferase reporter constructs were harvested and lysed using the Passive Lysis Buffer provided in the Dual-Luciferase Reporter Assay System (Promega, cat #E1980). Relative activities of the firefly and renilla luciferase domains in the clarified lysate were then measured using the Dual-Luciferase Reporter Assay System on a Synergy Neo2 plate reader (Biotek ...
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bioRxiv - Bioengineering 2022Quote: CYP3A4 activity of hLiMTs in monoculture or in co-culture with MSCs was assessed using the P450-Glo CYP3A4 Assay (Promega, Dübendorf, Switzerland) with luciferin isopropyl acetal (luciferin-IPA ...
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bioRxiv - Microbiology 2023Quote: ... and CytoTox 96® Reagent was added for 30 minutes according to manufacturers’ instructions (CytoTox 96® Non-Radioactive Cytotoxicity Assay, Promega). After adding Stop Solution ...
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bioRxiv - Immunology 2023Quote: ... Cell samples were collected at the indicated times and lysed or assayed using the Dual-Luciferase® Reporter Gene (DLR™) Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability was evaluated 72 hours later by measuring adenosine triphosphate (ATP) levels using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega, G7572) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were incubated at 37°C for 24 h before proliferation was assayed by Hoechst staining or WST-1 and luciferase signal was measured by Steady-Glo® Luciferase Assay System (Promega, E2520).
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bioRxiv - Cancer Biology 2023Quote: ... Luminescence was measured 24 h after plating (T0) and after 4 d using the CellTiter-Glo Luminescent Cell Viability Assay protocol (Promega; cat# G7573). When indicated ...
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bioRxiv - Bioengineering 2023Quote: ... the liquid DMEM culture medium and the hydrogel overlaying the cultured chondrocytes were first removed before introducing 300 µL of CellTiter 96® Aqueous One Solution Cell Proliferation assay (Promega, Australia) working solution ...
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bioRxiv - Microbiology 2023Quote: ... The amount of produced pseudovirus particles was quantified by the HiBiT assay using Nano Glo HiBiT lytic detection system (Promega, Cat# N3040) as previously described14 ...
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bioRxiv - Biochemistry 2023Quote: The cell viability of isogenic RCH-ACV cell lines treated with NSD2 degrader and dexamethasone was determined by the CellTiter-Glo® Luminescent Cell Viability Assay (Promega, #G7572). The opaque-walled 96-well plates with cells were equilibrated at room temperature before detection ...
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bioRxiv - Cell Biology 2024Quote: ... and 25 μl of lysate was analyzed using the protocol described in the Nano-Glo® Dual-Luciferase® Reporter Assay System (Promega).
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bioRxiv - Plant Biology 2024Quote: ... and FUL1 were cloned into a plant expression vector as effectors.Using transient transformation of tobacco leaves and the Dual-Luciferase reporter assay system (Promega, Madison, WI, USA), we tested the ratio of LUC to REN to determine the activation of the SlVI promoter by these transcription factors.
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bioRxiv - Microbiology 2024Quote: ... Pseudovirus titers were measured by infecting target cells for 48 h prior to measuring luciferase activity (luciferase assay reagent, Promega, Madison, WI), as described previously (58) ...
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bioRxiv - Microbiology 2024Quote: ... and the amount of intracellular ATP was measured using the CellTiter-Glo assay according to the manufacturer’s protocol (Promega, Madison, WI, USA).
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bioRxiv - Genomics 2020Quote: DNA was extracted from the fin clips of the challenged fish using a commercial kit (Wizard Genomic DNA Purification Kit, Promega), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2019Quote: ... Genomic DNA from Legionella was isolated using the Wizard Genomic DNA Purification Kit or the Maxwell DNA extraction kit (Promega). Plasmids from L ...
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bioRxiv - Genomics 2019Quote: ... The genomic DNA and total RNA were extracted from cells using the Maxwell 16 Cell LEV DNA Purification Kit and the Maxwell 16 Cell LEV Total RNA Purification Kit (Promega), respectively.
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bioRxiv - Molecular Biology 2019Quote: ... collected by scraping and lysed in the buffer provided by the Nucleospin RNA isolation kit (Mackerey-Nagel) or the Reliaprep miniprep kit (Promega). RNA was isolated according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... paraffin-embedded (FFPE) tissue slides using Maxwell® RSC Blood DNA Kit and Maxwell® RSC RNA FFPE Kit (Promega) following the manufacturer’s recommendations ...
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bioRxiv - Plant Biology 2022Quote: ... purified with a NucleoSpin® Gel & PCR Clean-up kit (Machery-Nagel) and radiolabeled dCTP-P32 were incorporated with a Prime-a-gene Labeling System kit (U1100, Promega). Oligonucletotide probes were ordered from GenoScreen and radiolabeled dATP-P32 were incorporated with a T4 Polynucleotide Kinase kit (T4 PNK EK0031 ...
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bioRxiv - Neuroscience 2024Quote: Total RNA was extracted from FACS purified iPSC-neurons and frozen mouse brain tissue using the ReliaPrep RNA cell kit and ReliaPrep RNA tissue extraction kit (Promega) respectively.
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bioRxiv - Microbiology 2024Quote: ... difficile was mixed with 50 µL kit reagents (100-fold diluted LgBiT protein, 50-fold diluted substrate in kit buffer, Promega). Subsequently ...
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bioRxiv - Microbiology 2023Quote: ... gallolyticus genomic DNA was extracted using either Wizard Genomic DNA Purification Kit or Wizard SV Genomic DNA Purification Kit (Promega). The extraction was performed according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was then amplified with the GenomiPhi V2 DNA Amplification Kit (Cytiva Life Sciences) and again quantified using the Quantus Fluorometer and QuantiFluor dsDNA Kit (Promega). Illumina DNA Prep (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids were extracted using a standard Qiagen Miniprep kit and genomic DNA (gDNA) was extracted using a standard Wizard DNA Extraction Kit (Promega) and all DNA was quantified using a Qubit ...
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bioRxiv - Microbiology 2020Quote: ... plates were incubated at 37°C for 72 hours prior to assessing luciferase activity using the Renilla-Glo Luciferase Assay System (Promega, Madison, WI, USA). Readout of eGFP was done by incubating and monitoring plates at 37°C for 72h in an IncuCyte® (Essen BioScience Inc. ...
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bioRxiv - Developmental Biology 2021Quote: ... The embryos were ground in an eppendorf tube with a pestle in 1x passive lysis buffer and luciferase signal was measured using the Dual Luciferase assay from Promega (Promega, Madison, WI). tll transgenes were similarly cloned with Gateway LR Clonase (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Dual-luciferase assays were performed using 40 μl of the substrate in the appropriate buffer for a 10 μl in vitro translation assay (Promega, Cat. No. E1960). Luminescence was measured using TECAN Infinite M1000 and plotted on GraphPad (Version 8.4.2).
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bioRxiv - Cancer Biology 2022Quote: ... The organoids were incubated for 5 days at 37C and assayed using the CellTiter-Glo® 3D Cell Viability Assay (Promega Cat # G9681) and read for luminescence using the EnVision 2105 plate reader ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell numbers were quantified using a colorimetric 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS)-based CellTiter 96® AQueous One Solution Cell Proliferation Assay (Promega, Madison, WI) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Pseudovirus titers were measured by infecting 293T/TRCAT cells for 72 hours before measuring luciferase activity (ONE-Glo™ Luciferase Assay System, Promega, Madison, WI). Pseudovirus titers were expressed as relative luminescence units per milliliter of pseudovirus supernatants (RLU/ml) ...
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bioRxiv - Molecular Biology 2019Quote: ... The transfected cells were treated with various reagents for 24 hours and then lysed and assayed for luciferase activity using the Luciferase Assay System (Promega Corp., Madison, WI) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2020Quote: ... Cell extracts were prepared 48 hours after transfection and luciferase activity was measured using the Dual-Luciferase Reporter assay system (Promega, Madison, WI, USA). Renilla luciferase served as an internal control for normalization.
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bioRxiv - Developmental Biology 2019Quote: ... We measured LacZ reporter activity using the Beta-Glo® Assay System following the commercially available protocols and the yeast literature (Promega, Madison, WI) (Hook ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Cells were harvested 3 days after transfection and Firefly and Renilla luciferase activities were measured by using a Dual-Luciferase Reporter Assay System (Promega, Cat. No. E1910). The mean values +/- SD from five independent experiments are shown.
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... The toxicity of each chemicals was tested on each cell line using the CellTiter-Glo Luminescent Cell Viability Assay (Promega, Madison WI, USA).
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bioRxiv - Cell Biology 2021Quote: ... Firefly luciferase and the Renilla control were detected using the Promega Dual-Luciferase Reporter Assay System per the manufacturer’s protocol (Promega, Madison, WI, cat# E1960). Plates were read on the Victor Nivo plate reader (Perkin Elmer ...
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bioRxiv - Microbiology 2020Quote: ... Cell viability in the presence of the drugs (without infection) was determined based on cellular ATP content using the CellTiter-Glo® Luminescent Cell Viability Assay (Promega, cat#: G7572) according to the manufacturer’s instruction.
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bioRxiv - Microbiology 2021Quote: ... Membrane fusion activity was quantitatively assessed by detecting the expression of firefly luciferase and sea pansy luciferase using the Dual-Luciferase Reporter Assay System (Promega, Madison, WI, USA) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2022Quote: ... Cell viability was determined by removing 100 μL supernatant from all wells and adding 100 μL CellTiter-Glo reagent to the cells as described in the manufacturers protocol (CellTiter-Glo® Luminescent Cell Viability Assay, Promega, Madison, USA). After 2 minutes shaking on an orbital shaker ...
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bioRxiv - Developmental Biology 2022Quote: ... Dual-luciferase assays were performed using the Promega Dual-Luciferase Reporter (DLR™) Assay Systems with the Promega GloMax 20/20 Luminometry System (Promega, Madision, WI) (Stepicheva et al. ...
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bioRxiv - Bioengineering 2022Quote: Induction of apoptosis after 24 h of doxorubicin treatment was quantified by analyzing Caspase 3/7 activity using the Caspase-Glo® 3/7 3D assay (Promega, Madison, WI). The assay protocol was followed from the manufacturer’s website ...
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Farnesyltransferase inhibition overcomes the adaptive resistance to osimertinib in EGFR-mutant NSCLCbioRxiv - Cancer Biology 2022Quote: Viability of untreated cells or treated cells for 5 days was assessed using CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (Promega®, ref:G3580) according to manufacturer’s instructions.