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Citations for Promega :
5851 - 5900 of 6889 citations for Mouse Translational Activator Of Cytochrome C Oxidase 1 TACO1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... with 25 μL of diluted reactions (diluted 1:5 in Glo Lysis Buffer; Promega # E2661) and incubated at room temperature for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... followed by overnight digestion with trypsin (enzyme to protein ratio of 1:100) (Promega, Germany) at 37 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used included alkaline phosphatase-conjugated anti-rabbit IgG antibody (1:1,000, #S3738, Promega) and anti- mouse IgG antibody (1:1,000 ...
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bioRxiv - Cell Biology 2023Quote: ... Sequencing of the gRNA-targeted exon 1 was performed by PCR amplification (Promega GoTaq polymerase) with 5’-GGTTTGGGGCTGGGCAT-3’ and 5’-AGGTGCAGCAGCAGTACG-3’ primers (Guillen-Samander et al. ...
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bioRxiv - Genomics 2023Quote: ... 1 μg of total RNAs was reverse-transcribed using ImProm-IITM Reverse Transcription System (Promega) and genes were analysed with Quantifast SYBR green master mix (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... 1 mM DTT) buffer and luminescence activity was measured with the Luciferase Assay System (Promega).
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bioRxiv - Microbiology 2023Quote: Viral loads were quantified using the GoTaq® Probe 1-Step RT-qPCR System (Promega). For quantification of SARS-COV-2 the nCOV_N1 primer/probe mix from the SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Biophysics 2023Quote: ... Cross-linked samples for XL-MS were digested by 1:50 (m/m) trypsin (Promega) overnight at 37 °C while shaking at 600 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... RNA (1 μg per reaction) was reverse transcribed using the GoScript Reverse Transcription System (Promega). Following reverse transcription ...
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bioRxiv - Immunology 2023Quote: ... Complementary DNA (cDNA) was generated using 1 μg RNA using M-MLV Reverse Transcriptase (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: High concentration (1 µg/µL) plasmid DNA was extracted via maxi-prep (Promega SV Wizard). Lower quality plasmid DNA (e.g ...
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bioRxiv - Microbiology 2023Quote: ... 0.25 µl 1 M MgCl2 and 0.3 µl Rnasin ribonuclease inhibitor (Promega, 0.4 u/µl) in a final volume of 30 µl at 25°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Bound antibodies were detected using the appropriate horseradish peroxidase-conjugated secondary antibodies (1:10000, Promega). Chemiluminiscent signals were detected with an ECL Prime Western blotting detection kit (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG antibody (1:10000) (Promega, Madison, WI, USA), diluted in 3% (w/v ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were again diluted to 2 M urea and digested with 1 µg trypsin (Promega) (1/100 ...
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bioRxiv - Biochemistry 2024Quote: ... Then membranes were incubated with secondary anti-rabbit IgG horse radish peroxidase (Promega, 1:5000) for 1 hour ...
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bioRxiv - Systems Biology 2023Quote: The transfections were performed with a 1 mg DNA: 2 ml Fugene HD (Promega E2311) ratio ...
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bioRxiv - Biochemistry 2023Quote: ... The blot was probed with 1:1000 dilution of anti-NLuc antibody (Promega cat#7000) and the bands detected by chemiluminescent detection.
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bioRxiv - Cell Biology 2024Quote: ... and samples were digested with 1:15 mass ratio of Sequencing Grade Modified Trypsin (Promega) using an S-trap mini device (Protifi ...
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bioRxiv - Cancer Biology 2024Quote: ... Ten ml of conditioned media was treated with 1 ml Proteinase K (Promega Cat # V3021) in buffer (10 mM Tris-Cl pH8.0 ...
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bioRxiv - Biochemistry 2024Quote: ... pH 8.0) containing 2 nM 11S and 1/250 Nano-Glo Luciferase Assay Reagent (Promega) was added to the cells and background luminescence read for 5 min ...
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bioRxiv - Biochemistry 2024Quote: ... Trypsin digestion was performed with a 1 μg /μL trypsin solution (Trypsin Gold, V528A, Promega) at a ratio of 1:50 at 37 °C for 16 h ...
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bioRxiv - Molecular Biology 2024Quote: ... and then placed in a 50 mL solution of 1 ×Diamond Nucleic Acid Dye (Promega) in 0.2 ×TBE buffer pH 7.6 to rock in the dark for 30 min ...
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bioRxiv - Cancer Biology 2021Quote: Genomic DNA from cell lines was isolated using Maxwell® 16 Instrument and the Maxwell® 16 DNA Purification Kit (Promega Cat.# AS1020) following the protocol provided ...
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Differential polysaccharide utilization is the basis for a nanohaloarchaeon : haloarchaeon symbiosisbioRxiv - Microbiology 2019Quote: ... Amplicons for cloning were cut out from the gel and purified with the Wizard SV Gel and PCR Clean–up System kit (Promega, Madison, WI, USA). The other Archaea present in the last positive enrichment were identified following the cloning and sequencing of the 16S rRNA gene using the conventional archaeal universal primers A20F-1492R42,43 ...
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bioRxiv - Cell Biology 2022Quote: Proliferation assays were performed in 96 well plates (2 × 103 NMCCs/well) and assayed at 48 h using the CellTiter96® AQueous One Solution Cell Proliferation Assay kit (MTS; Promega, Madison, WI) (38) ...
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bioRxiv - Immunology 2022Quote: The NAD+/NADH ratios were performed according to the manufacturer’s instructions of the NAD/NADH-Glo™ Assay kit (Promega, Cat. No. G9071). Briefly ...
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bioRxiv - Genomics 2019Quote: Genomic DNA was isolated from 30 ul of chicken blood samples using the Promega Wizard Genomic DNA Purification Kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... while the activity of both firefly and Renilla luciferase reporters was determined 48 hours post transfection using the Dual Luciferase Assay kit (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... genomic DNA from puromycin selected cells and wild-type HEK293 cells was extracted (Promega Wizard Genomic DNA Purification Kit #A1120; Promega, Madison, WI, USA) for PCR amplification of a 535 bp region flanking the sgRNA cleavage site ...
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bioRxiv - Plant Biology 2019Quote: ... young and mature leaves of five plants (siblings of the plant used for genome sequencing) using the SV Total RNA Isolation Kit with on-column DNase I treatment (Promega, Madison, WI, USA). The RNA quantity and quality were assessed by RIN (RNA Integrity Number ...
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bioRxiv - Cell Biology 2021Quote: Cytochrome P450 3A4 (CYP3A4) enzyme activity assays were assessed by measurement of luciferase activity using P450-Glo CYP3A4 assay kit (Promega, Madison, WI, USA) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... of luciferase was determined using a PicaGene Luminescence Kit (TOYO B-Net Co., LTD, Tokyo, Japan) and GloMax Navigator System G2000 (Promega Corporation, Madison, WI), according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... and DNA-concentration was determined using a QuantiFluor® dsDNA kit (detection limit: 50 pg/mL; sensitivity: 0.01 – 200 ng/µL) and Glomax® –Multi+ system (Promega, Madison, WI) with the blue fluorescence optical kit installed (Ex ...
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bioRxiv - Microbiology 2020Quote: ... Extraction of total RNA from viral transport media was performed using the Maxwell RSC Whole blood RNA/DNA kit (Promega Corporation, Madison, WI) on the Maxwell RSC 16 automated nucleic acid extractor ...
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bioRxiv - Microbiology 2020Quote: Faecal DNA was extracted and purified using a combination of repeated bead-beating (method 5) (Costea et al, 2017) and the Maxwell 16 Tissue LEV Total RNA Purification Kit (Promega, Maddison, WI, USA), with STAR (Stool transport and recovery ...
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bioRxiv - Plant Biology 2020Quote: ... and five early developmental buds (one early developmental sample) per individual using the Maxwell 16 LEV Plant RNA Extraction Kit (Promega Corporation, Madison, WI).
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bioRxiv - Molecular Biology 2020Quote: ... and then cloned to ensure representative amplification of the parental copies using pGEM-T Easy-cloning Vector Kit (Promega Corp., Madison, WI, USA) and transformed into competent Escherichia coli JM109 at 42 °C ...
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bioRxiv - Developmental Biology 2020Quote: ... DNAs were purified using the PureYield Midiprep kit and were linearized before in vitro synthesis of anti-sense RNA probes using T7 or Sp6 polymerase (Promega, #P2077 and #P108G), RNAse inhibitor and dig-labeled rNTPs (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... was measured after 48 and 72 h using the luminescence-based CellTiter-Glo®2.0 cell viability assay kit (Promega, Madison, WI, USA) according to the manufacturer’s instructions with an FLx100 Luminometer (CLARIOSTAR® ...
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bioRxiv - Cell Biology 2022Quote: ... The protein concentration was measured using Pierce™ BCA Protein Assay Kit and GloMax® Explorer Multimode Microplate Reader (Promega, Madison, WI, USA). Expression of OAT1 ...
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bioRxiv - Developmental Biology 2022Quote: ... cells were detached using AccutaseTM and RNA was extracted using the Promega kit ReliaPrep TM RNA Cell Miniprep System (Promega, US. Catalog# Z6011). RNA was reverse transcribed using Omniscipt TM RT kit (Qiagen ...
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bioRxiv - Bioengineering 2022Quote: ... and drug delivery method (IV and TACE) on CYP3A4 activity was tested with the P450-Glo CYP3A4 assay kit (V9001, Promega, Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: Healthy young leaves from each line were collected and genomic DNA was extracted using a Maxwell RSC Plant DNA Kit (Promega, Madison, WI, USA). The presence or absence of the 5.46-kb fragment containing DAI was confirmed by PCR (primers 1 and 2) ...
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bioRxiv - Cell Biology 2022Quote: ... was used as a template in PCR reactions and amplifications were performed with the GoTaq®G2 Flexi DNA Polymerase kit (Promega, Southampton, UK). Cycling parameters were one cycle of 95°C for 5 minutes for initial denaturation followed by touch-down cycling for the first 14 cycles ...
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bioRxiv - Microbiology 2020Quote: ... genomic DNA was isolated from the corresponding BKE (Bacillus Knockout Erythromycin collection - (Koo et al., 2017)) strains using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI). Of note ...
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bioRxiv - Cell Biology 2020Quote: ... 72 hours post induction the cells were assayed for CYP1A2 and CYP3A4 activity using the P450-Glo CYP3A4 (Luciferin-PFBE) Cell-based/Biochemical Assay kit (Promega, Cat. no. V8902) and the P450-Glo CYP1A2 Induction/Inhibition Assay kit (Promega ...
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bioRxiv - Genomics 2019Quote: ... amplicons from each replicate were pooled and purified with the Wizard SV Gel and PCR Cleanup System kit (Promega Corporation, Madison, Wisconsin, USA).
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bioRxiv - Cancer Biology 2019Quote: ... Cell lines were authenticated at the Duke University DNA Analysis Facility by analyzing DNA samples from each cell lines for polymorphic short tandem repeat (STR) markers using the GenePrint 10 kit from Promega (Madison, WI, USA). HCT116 cell line was a gift from Dr ...
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bioRxiv - Cancer Biology 2020Quote: ... Lipofectamine 3000 was used to co-transfect HEK-293T cells with wild-type and mutant luciferase reporter vectors as well as P51-ETS1-NC or P51-ETS1.Relative luciferase activity was expressed as the ratio of firefly luciferase activity to sea kidney luciferase activity using a dual luciferase reporting kit (Promega, Madison, Wisconsin, USA) after 48 h.