Labshake search
Citations for Promega :
5651 - 5700 of 8541 citations for Nitrite Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2020Quote: ... Transfected cells were collected in PBS and luciferase activity was assessed using the Nano-Glo Dual Luciferase reporter assay system (Promega). Dual luciferase signal was quantified using a VICTOR-3 plate reader (Perkin Elmer) ...
-
bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested 24 h after treatment and luciferase activity was measured using Luciferase Assay System (Promega, Leiden, The Netherlands). Infections were performed using pseudotyped particles obtained by co-transfecting HXB2 Env with pNL4.3.Luc.R-E-plasmid into HEK 293T cells using PEI ...
-
bioRxiv - Microbiology 2019Quote: ... differences in cell viability due to virus-induced cytopathic effect (CPE) or compound-specific side effects were analyzed using the CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay (Promega), as described previously (de Wilde et al. ...
-
bioRxiv - Microbiology 2019Quote: ... we measured the toxicity of the drugs in the exact same format by determining the number of viable cells utilizing the CellTiter 96 AQueous Non-Radioactive Cell Proliferation Assay (Promega) following the manufacturer instructions ...
-
bioRxiv - Genomics 2020Quote: ... firefly and Renilla luciferase luminescence was measured using the Dual-Glo® Luciferase Assay System (Promega Corporation, Madison, WI, USA). Firefly luminescence values were normalized to Renilla luminescence and compared to the empty pGL3::PRO vector.
-
bioRxiv - Immunology 2020Quote: ... The activity of Renilla luciferase in total cell lysates was measured with the use of a Dual-Luciferase Reporter Assay System (Promega) and was normalized by that of firefly luciferase derived from 80 ng of a control plasmid.
-
bioRxiv - Microbiology 2020Quote: ... towards HeLa cells was determined as a release of the intracellular enzyme lactate dehydrogenase (LDH) into the cell culture media using CytoTox 96 assay (cat.no. G1780, Promega, USA) or as changes in cell membrane integrity using CellTox Green Cytotoxicity Assay (cat.no ...
-
bioRxiv - Cell Biology 2020Quote: IC50 was determined on days 3 and 4 after vemurafenib treatment using Real-time-Glo MT Cell Viability Assay according to the manufacturer’s protocol (Promega, #G9711). Using a 96-well white plate ...
-
bioRxiv - Molecular Biology 2020Quote: ... Apoptosis was assessed by incubating the cells with 200nM Staurosporin or medium for 4 h and the caspase 3/7 activity was assayed using the ApoOne Caspase 3/7 Assay (Promega). Senescence associated β-Galactosidase activity was analyzed with the Senescence Associated β-Galactosidase Staining Kit (Cell Signalling Technologies) ...
-
bioRxiv - Molecular Biology 2020Quote: ... Luciferase activity present in 10 µl of lysate clarified by centrifugation was then quantitated in white 96-well luminometer plate (Microlumat Plus 1b 96V instrument, Berthold Technologies) using 50 µl of luciferase assay reagent (Promega).
-
bioRxiv - Systems Biology 2020Quote: ... cells were lysed and cellular luciferase activity was measured to evaluate the HCV replication activity with a Luciferase Assay System (Promega) per manufacturer’s protocol (18).
-
bioRxiv - Cancer Biology 2021Quote: ... Cell proliferation was measured every day for up to 72 hours with the CellTiter-Blue assay (#G8080, Promega, Madison, WI) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The numbers of cells that migrated into the lower chamber as well as those detached by Trypsin-EDTA were quantified by CellTiter-Glo (CTG) assay (Promega). % Migration = the number of cells that migrated in response to chemoattractant / the total number of cells seeded on the chamber.
-
bioRxiv - Developmental Biology 2020Quote: ... the cells for the luciferase assay were exposed to 100 μL of a 1:1 dilution of DMEM and luciferase substrate (ONE-Glo Luciferase Assay System, Promega), and luminescence from each well measured in a GloMax-96 Microplate Luminometer (Promega) ...
-
bioRxiv - Microbiology 2020Quote: ... All drugs were dissolved in dimethyl sulfoxide (DMSO) and their cytotoxicity determined using a lactate dehydrogenase (LDH) assay (Promega, UK).
-
bioRxiv - Microbiology 2020Quote: ... by counting the number of green cells in each well using an Acumen eX3 scanner or by measuring luciferase activity with Steady-Glo Luciferase assay system (Promega) using EnVison (Perkin Elmer).
-
bioRxiv - Microbiology 2021Quote: ... The cytotoxicity of compounds (100 uM to 1 uM, 3-fold dilution) was examined using the CellTiter-Glo Luminescent Cell Viability Assay (Promega). Cell cytotoxicity data was normalized to DMSO control as 0% cell death.
-
bioRxiv - Microbiology 2021Quote: ... Sister plates were exposed to drug but not infected to gauge cytotoxicity via CellTiter-Glo assay (CTG, Promega, Madison, WI), 48 hrs post treatment.
-
bioRxiv - Cell Biology 2021Quote: Proteasome activity was measured in iPSC-CMs using the Proteasome-Glo™ Chymotrypsin-Like Assay according to manufacturer’s instructions (Promega). Cells were incubated with the inhibitors (ranging from 2 to 5000 nM ...
-
bioRxiv - Immunology 2020Quote: ... the cells were lysed and luciferase activity was measured in the lysates using the Nano-Glo Luciferase Assay System (Promega). Relative luminescence units (RLU ...
-
bioRxiv - Pathology 2021Quote: ... induction of apoptosis was determined by measurement of caspase-3/7 activity using the luminometric Caspase-Glo 3/7 assay (Promega) according to the manufacturer’s protocol and a microplate reader (Microplate Reader SH900) ...
-
bioRxiv - Physiology 2019Quote: ... cells were harvested for the determination of Firefly and Renilla luciferase activity using the dual-luciferase reporter assay system (Promega), and Firefly luciferase activity was normalized to Renilla luciferase activity ...
-
bioRxiv - Molecular Biology 2020Quote: ... Western blots to assess the abundance of the proteins were performed on the same volumes of lysates obtained from the luciferase measurements using the passive lysis buffer supplied with the Dual-Luciferase Reporter Assay System (Promega). Antibodies used for western blots were previously described (Grozdanov et al. ...
-
bioRxiv - Biochemistry 2019Quote: ... for the indicated times at 37 °C and activity was measured using the Dual-Glo Luciferase Assay System (Promega, E2940) in a Tecan Infinite M200 Pro multi-well plate reader ...
-
bioRxiv - Developmental Biology 2020Quote: ... Reporter plasmids were constructed by inserting the mouse Olig2 gene mR2 and mR6 regions identified by the ChIP assays in situ into the pGL3-Basic vector (Promega), respectively ...
-
bioRxiv - Neuroscience 2019Quote: ... The samples were then cleared by centrifugation and the supernatant was diluted (1/50) with extraction buffer and mixed with the luminescent solution (CellTiter-Glo Luminescent Cell Viability Assay, Promega). The luminescence was measured on an Infinite M200PRO plate reader (TECAN ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were lysed with passive lysis buffer 48 hours after infection and reporter activity was then measured with a Nano-Glo luciferase assay system (Promega). For IFN-I based experiments increasing amounts of IFN-I were added 16 hours before infection and for TRIM25 overexpression experiments cells were reverse transfected with either TRIM25 or YFP as well as TIM1 24 hours before infection.
-
bioRxiv - Cancer Biology 2021Quote: Cells were seeded at a concentration of 3×105/mL in RPMI medium on day 0 and the number of cells was measured every two-three days using the MTS assay from Promega following the manufacturer’s protocol ...
-
bioRxiv - Cell Biology 2020Quote: ... After 24 hours the cells were washed briefly with 1x PBS prior to measurement of luciferase activity by Dual Luciferase Reporter Assay (Promega).
-
bioRxiv - Cancer Biology 2020Quote: ... Induction of ISRE-Luc reporter was evaluated by measuring Firefly and Renilla luciferase activity with dual-luciferase reporter assays on Glomax-Multi Detection System (Promega). The results were presented as relative light units (RLU) ...
-
bioRxiv - Cancer Biology 2021Quote: The LC50 values for the drugs (MTX, CPT and TDX) were determined by CellTiter-Blue® cell viability assay (Promega). Cells were seeded into 96 plates at a density of 3000 cells per well ...
-
bioRxiv - Zoology 2021Quote: ... The cells transfected with different recombinant vectors were cultured for additional 36 h at 37 °C for transcriptional activity analysis by using the Dual-Glo Luciferase Assay System (Promega) with a luminometer (Promega ...
-
bioRxiv - Biochemistry 2020Quote: ... the culture medium was aspirated and 20 μL of cell lysate was added from the Luciferase Assay System (Promega, E1500) to each well ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were exposed to the compounds for 72h and then processed for CellTiter-Glo® Luminescent Cell Viability Assay (Promega) readout according to manufacturer’s instructions and read on EndVision (Perkin Elmer) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Gene expression in the tumor was analyzed by using human primers using SYBR green gene expression assays (GoTaq qPCR Master Mix, A6002, Promega).
-
bioRxiv - Cancer Biology 2020Quote: ... Cell viability for 3 biological replicates of each treatment condition was assessed after 72 hours after drug treatment using the CellTiter-Glo Luminescent Cell Viability Assay (G7571, Promega) and an EnVision Multilabel Plate Reader (PerkinElmer) ...
-
bioRxiv - Physiology 2021Quote: ... the firefly luciferase signal of the unk-reporter and the renilla luciferase signal were quantified using the Dual-Glo Luciferase Assay system (Promega) following the manufacturer instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... assessed every day for 4 days by measuring ATP content through Cell Titer-Glo® Luminescent Cell Viability assay (Promega), was compared to cell viability assayed at day 1 ...
-
bioRxiv - Cancer Biology 2021Quote: ... Cell lysates were prepared by addition of 0.3N hydrochloric acid then 450nM Tris (pH=8) and metabolites were analyzed using the Glutamine/Glutamate Glo Assay (Promega; J8021) per manufacturer’s instruction.
-
bioRxiv - Genetics 2020Quote: ... Transfected cells were assayed after 48 h (as indicated for firefly and Renilla luciferase activity using the Dual-Luciferase Reporter Assay System (Promega) in a GloMax® Explorer System (GM3500).
-
bioRxiv - Cancer Biology 2021Quote: ... Cell number was measured after 3 and 7 days and normalized to the initial reading at day 0 using the CellTiter Glo Luminescent Cell Viability Assay (Promega). The experiments shown represent fold change at day 7 relative to day 0 ...
-
bioRxiv - Microbiology 2021Quote: ... 0.5 ml of culture supernatants were collected every 6 hours and nanoluciferase activities associated with released virus in the supernatants were measured using Nano-Glo luciferase assay system (Promega).
-
bioRxiv - Immunology 2020Quote: The fluorescent fusion assay was adapted to allow quantitative measurement of cell-cell fusion by leveraging the NanoBiT complementation system (Promega). Donor HEK293 cells were transfected with full-length S and the 11S subunit in 96-well white flat bottom TC-treated microtest assay plates ...
-
bioRxiv - Microbiology 2020Quote: ... IRES activity was determined by measuring Renilla luciferase (RLuc) and firefly luciferase (FLuc) activities using the Dual Luciferase Reporter Assay System (Promega).
-
bioRxiv - Microbiology 2020Quote: Cytotoxicity of macrophages after 8 hours of infection was quantified by measuring the release of the enzyme lactate dehydrogenase in cell supernatants using the CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2020Quote: ... cells were washed in 1 x PBS and cell viability was assessed using CellTiter-Glo Luminescent Cell Viability Assay (Promega). Luminescence read-out was normalized to signal from vehicle treated cells and represented as percentage of viable cells compared to vehicle ...
-
bioRxiv - Immunology 2021Quote: Cell death was assessed by measuring lactate dehydrogenase (LDH) release into the supernatant using a CytoTox 96 Non-Radioactive Cytotoxicity Assay (Promega) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2021Quote: ... 100 mL of media was removed and replaced with 100 mL of Bright-Glo Luciferase Assay Reagent (Promega, Cat # E2620). Luciferase activity was measured on the EnSpire Plate Reader (PerkinElmer) ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were lysed and luciferase activity was assessed using the Luciferase Assay System and the Glomax 20/20 luminometer (Promega). Infectivity values were analyzed and plotted using Prism 8.
-
bioRxiv - Molecular Biology 2021Quote: Cells were harvested two days after transfection and firefly and Renilla luciferase activities were measured using the Dual-Luciferase reporter assay system (Promega). Total RNA was isolated using TriFast (Peqlab biotechnologies) ...