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Citations for Promega :
5601 - 5650 of 9065 citations for Total Carbohydrate Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... were added to the 5′ end labelled 187cspA RNA in a total volume of 10 µL in Buffer O containing 0.4 U/µL of RNasin (Promega). Complex formation was performed at 15°C or 37°C for 15 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Complementary DNA (cDNA) was synthesized from 1 μg of total RNA using random primers and Reverse Transcriptase System (Promega). The cDNA was used as a template for the real-time PCR amplification using PowerUp SYBR® Green Master Mix and a StepOne Plus system (Applied Biosystem ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA from splicing reactions (∼20 fmol pre-mRNA in 10.5 µL splicing reaction) was analyzed by primer extension (15 µL reaction volume total) using AMV reverse transcriptase (Promega) as previously described (Mayas et al ...
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription was performed on total RNA using the GoScript Reverse Transcription System with an oligo-dT primer (Promega). Splicing was analyzed by radiolabeled PCR of resulting cDNA using GoTaq Green (Promega ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was synthesized using 5μg of total RNA at 42 °C for 2 hours using avian myeloblastosis virus (AMV) reverse transcriptase (Promega). A 20 µL reaction containing 10 ng or 10 pg (for 16S rRNA ...
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bioRxiv - Cell Biology 2021Quote: cDNA synthesis was performed using 1-5 μg of total RNA with random hexamers and reverse transcriptase (Promega, GoScript). Quantitative RT-QPCR was performed in triplicate for each samples using the KAPA SYBR PCR kit (Sigma Aldrich KK4600 ...
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bioRxiv - Developmental Biology 2020Quote: ... The cDNA was synthesized from 1 μg of the total RNA using the ImProm-II Reverse Transcription System (Promega) and oligo (dT)23 primers.
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bioRxiv - Immunology 2020Quote: ... A total of 0.8 μg RNA from different treatments was reversely transcribed into cDNA using M-MLV reverse transcriptase (Promega) with an oligo (dT ...
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bioRxiv - Neuroscience 2020Quote: A total of 100 μg protein was digested with trypsin (Trypsin Gold, Mass Spec Grade, Promega, Fitchburg, WI, USA) using filter-aided sample preparation methods [19] ...
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bioRxiv - Microbiology 2022Quote: ... First-strand cDNA generation from total RNA was generated using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega). Relative mRNA quantitation was done using the SYBR green quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Evolutionary Biology 2019Quote: ... Synthesis of cDNA was performed with 1 μg of total RNA by using the GoScriptTM Reverse Transcription System (Promega), according to the manufacturer’s recommendations ...
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bioRxiv - Physiology 2019Quote: Total RNA was extracted from zebrafish brain and intestine samples using an automatic system (Maxwell® 16 Instrument, Promega) and a total RNA purification kit (Maxwell® 16 LEV simplyRNA Tissue) ...
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bioRxiv - Plant Biology 2019Quote: ... PCR amplifications were carried out in a total reaction volume of 10μL containing 5 μL of 2xGoTaq master mix (Promega) and 2.5 μL of 4M betaine ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcriptase reaction was performed from 2 µg of total RNA by using ImProm-IITM Reverse Transcription System (Promega). Aliquots of cDNA obtained were amplified in PCR and quantitative real-time PCR reactions ...
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bioRxiv - Plant Biology 2020Quote: ... Five hundred ng total RNA was reverse transcribed using oligo(dT) and the MLV reverse transcription system following the manufacturer’s instructions (Promega). qRT-PCR was performed in 25 μL reaction volumes with gene-specific primers and conducted with a StepOne Plus Real-time PCR System (Applied Biosystems ...
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bioRxiv - Plant Biology 2020Quote: ... template cDNA samples were prepared using 4 κg of total RNA and the Improm-II reverse transcription system (Promega). Primers were designed to have a Tm of ~60°C and to produce PCR products of ~200−400 base pairs ...
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bioRxiv - Immunology 2019Quote: ... the indicated plasmids were mixed with an empty vector to a total of 2.0 μg DNA in 125 μl in Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2021Quote: ... Tissues were homogenized using ISOGEN (NIPPON GENE, Tokyo, Japan) and the SV Total RNA Isolation System (Promega, Madison, WI) was used for RNA extraction ...
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bioRxiv - Molecular Biology 2020Quote: ... Reverse transcription was performed on total RNA using the GoScript Reverse Transcription System with an oligo-dT primer (Promega). Splicing was analyzed by radiolabeled PCR of resulting cDNA using GoTaq Green (Promega ...
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bioRxiv - Genomics 2020Quote: ... 4 pmol probe mix was combined with 0.5 or 100 ng of total RNA along with 4U µl RNasin Plus (Promega) and Ribohyb buffer ...
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bioRxiv - Genomics 2021Quote: ... RNA was extracted using the Promega SV total RNA extraction system with modified protocol (Promega; Coolon et al. 2013). RNA quality was determined using gel electrophoresis and NanoDrop ...
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bioRxiv - Genomics 2021Quote: The total amount of enriched DNA released from the dMDA droplets was measured with Quantus™ Fluorometer (Promega Inc.) and fragment size distribution was inspected on TapeStation™ (Agilent ...
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bioRxiv - Cancer Biology 2022Quote: PCR to amplify the region flanking the editing site of COPA (Ile164Val) was performed in a total volume of 50μl containing 1x Green Go TaqR Flexi Buffer (Promega), 2 mM MgCl2 solution ...
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bioRxiv - Immunology 2022Quote: ... the indicated plasmids were mixed with an empty vector to a total of 2.0 µg DNA in 125 µL Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... the indicated plasmids were mixed with an empty vector to a total of 2.0 µg DNA in 125 µL Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2022Quote: Complementary DNA was prepared from total RNA extracted from indicated samples using the ImProm-II Reverse Transcription System (Promega). qRT-PCR analysis was performed using a master mix with ROX reference dye (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... and the scrambled EK1 control were combined (~100 nM total final concentration) and translated in 50 µL reactions containing 12.5 µM supplemental tRNA (Promega), 50 U of Superase-In (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... were transfected 2 days after plating with 1 μg of total DNA with FuGENE 6 transfection reagent (E2691; Promega) at a ratio 1:3.
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bioRxiv - Molecular Biology 2024Quote: ... Total RNAs were collected from a 10-cm dish directly in 400 μl of 1x Reporter lysis buffer (Promega) containing 0.16 U/μl Ribosafe RNase inhibitors (Bioline) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Total RNA was purified of genomic DNA contamination using DNase (RQ1 RNAse-free DNAse, Cat. # M610A, Promega, Madison, WI) and reverse transcribed using the High Capacity cDNA Reverse Transcription Kit (Cat.# 4368814 ...
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bioRxiv - Cell Biology 2024Quote: ... reverse transcription was carried out using 1 μg of total RNA and random primers (Reverse Transcription System, Promega, A3500). IQTM SYBR® Green qPCR Supermix (Bio-Rad ...
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bioRxiv - Immunology 2023Quote: ... the indicated plasmids were mixed with an empty vector to a total of 2.0 μg DNA in 125 μL Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized using 1μg total RNA with 200 units moloney murine leukemia virus reverse transcriptase (Promega, Southampton UK), primed with 2μM oligo dT (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: ... total RNA was extracted from cultured cells using the Maxwell RSC simplyRNA Cells system (AS1390, Promega, Madison, WI, USA), then reverse transcribed into cDNAs with the iScript cDNA synthesis kit (Bio-Rad) ...
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The tetrapeptide sequence of IL-1β regulates its recruitment and activation by inflammatory caspasesbioRxiv - Immunology 2023Quote: ... the indicated plasmids were mixed with a control RFP vector to a total of 1.0 μg DNA in 65 μL Opti-MEM and transfected using FuGENE HD (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was used for first-strand cDNA synthesis with the Reverse Transcription System I (Promega, Madison, WI, USA), then diluted 1:50 in water ...
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bioRxiv - Genomics 2023Quote: ... Total RNA from ED and TE samples was isolated using the ReliaPrepTM RNA Cell Miniprep System (Promega, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... cDNAs were synthesized from 500 ng of total RNA for each sample using M-MLV reverse transcriptase (Promega, M1701) and (dT)25 according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: RNA samples from two biological replicates were pooled and total RNA was treated with DNase (1 U/µl, Promega). For cDNA synthesis ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from each thrombus using a ReliaPrep RNA tissue MiniPrep System (Z6110, Promega, Madison, WI, USA) and from whole blood using a PAXgene Blood RNA Kit (762164 ...
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bioRxiv - Cancer Biology 2021Quote: ... residual viability was assessed by measuring ATP content through Cell Titer-Glo® Luminescent Cell Viability assay (Promega).
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bioRxiv - Cancer Biology 2021Quote: ... the expression of CYP3A4 was quantified using P450-Glo™ Assay and Screening Systems (V9001, Promega, Madison, WI) in response to different oxygen concentrations ...
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bioRxiv - Microbiology 2020Quote: ... and 24 hours after media changing the infection was quantified by luciferase detection with BrightGlo luciferase assay (Promega) and read in a Victor3 plate reader (Perkin Elmer ...
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bioRxiv - Systems Biology 2020Quote: Cell viability after heat shock and during recovery was estimated with CellTiter-Glo Luminescent Cell Viability Assay (Promega). Cells were pelleted ...
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bioRxiv - Cell Biology 2020Quote: ... and lysate was either measured directly for GFP signal or incubated with luciferase assay reagent LAR (Promega, E1500) for luminescence signal ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were then lysed and luciferase activities were measured using a Dual-Luciferase Reporter Assay System (Promega) on a Veritas luminometer (Turner BioSystems ...
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bioRxiv - Cell Biology 2020Quote: ... Luciferase activity was measured 48 hrs after transfection using the Dual-Luciferase® Reporter Assay System (Promega, E1910). Plate reader VICTOR™ X4 was used to measure Firefly and Renilla luminescence ...
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bioRxiv - Cell Biology 2020Quote: ... and luciferase activity was determined in cell extracts using the Dual-Luciferase reporter assay system (Promega, Madison, WI). Each experiment was performed in triplicate ...
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bioRxiv - Immunology 2021Quote: ... After 24h of incubation cell infection was measured by luciferase assay using Bright-Glo™ Luciferase System (Promega) Infinite F200 plate reader (Tecan ...
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bioRxiv - Genetics 2021Quote: ... Cultures were incubated for 24 hrs prior to harvest for dual luciferase assay as per manufacturers instructions (Promega).