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Citations for Promega :
5551 - 5600 of 8213 citations for QuantiChrom Indican Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... cells or biofilms were resuspended in 1x PBS (Difco) measurements carried out using BacTiterGlo™Microbial cell viability Assay (Promega) in accordance with manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... cells were washed with phosphate-buffered saline (PBS) and lysed for measuring luciferase activity by the Dual-Luciferase Reporter Assay System (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The plates were then incubated at 37°C overnight before the quantification of luciferase activity using the Luciferase Assay System (Promega) on SoftMax Pro v7.0.2 (Molecular Devices) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were harvested 48 hours after transfection and were assayed for the luciferase activity using the luciferase assay system (Promega) and a luminometer (BMG LABTECH ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were left to grow for 48 hrs of treatment (unless indicated otherwise) and then cytotoxicity was measured using the CellTiter-Glo Luminescent Cell Viability Assay (Promega) according to manufacturer protocol with a Molecular Devices Spectramax M3 plate reader.
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bioRxiv - Genomics 2024Quote: ... Renilla luciferin reagent (25 μl of Stop & Glo Reagent, Promega Dual-Luciferase Reporter Assay System; Promega, Madison, WI, cat. #E1910) was injected into each well through one of the injectors of a CentroXS3 instrument (Berthold Technologies) ...
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bioRxiv - Genetics 2024Quote: ... HEK293T cells cultured in a 12-well plate were harvested and processed according to the instruction of Dual-Glo Luciferase Assay System (Promega) with minor modification ...
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bioRxiv - Synthetic Biology 2024Quote: ... and then transferred to 96-well plates (white with a clear flat bottom) containing Nano-Glo Luciferase Assay Reagent (Promega) diluted 1000-fold in PBS at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... Bioluminescence measurements were taken by combining 25 µl of the sample solution with an equal volume of prepared buffer-reconstituted nluc substrate as detailed by the manufacturer (Nano-Glo Luciferase Assay System; Promega). Measurements were taken every 10 min in Nunc™ F96 MicroWell™ 446 plates using a GloMax® navigator luminometer (Promega ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Plates were then incubated for 72 h at 37°C and 5% CO2 and cell viability determined by CellTiter-Glo Luminescent Cell Viability Assay (Promega). In short ...
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bioRxiv - Immunology 2024Quote: ... The supernatants were used to determine lactate dehydrogenase activity and IL-1β level using LDH-GloTM Cytotoxicity Assay (Promega, J2380) and Mouse IL-1 beta DuoSet ELISA (R&D Systems ...
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bioRxiv - Plant Biology 2024Quote: ... benthamiana leaves were harvest after 48 hr of agroinfiltration99 and luciferase activity was quantified using the dual-luciferase reporter assay system (Promega). To determine the relative promoter activity ...
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bioRxiv - Microbiology 2024Quote: ... Pseudovirus titers were determined by infecting TZM-bl-luciferase reporter cell lines and estimating 50% tissue culture infectious dose (TCID50) using the Bright-Glo luminescence reporter gene assay system (Promega)73 as directed by the manufacturer.
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bioRxiv - Genetics 2022Quote: Genomic DNA was isolated from blood samples using commercial kit (Wizard® Genomic DNA Purification Kit; Promega), following the fabricant protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... The endpoints of the second screen were caspase 3/7 activity measured using the CaspaseGlo® 3/7 assay reagent (Promega) at the 8-hour and 16-hour time intervals ...
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bioRxiv - Neuroscience 2021Quote: Cell cytotoxicity was detected by measuring lactate dehydrogenase (LDH) levels using the CytoTox 96® Non-Radioactive Cytotoxicity Assay (Promega, G1780). The manufacturer’s instructions were followed and the absorbance reflecting the LDH content in the cell supernatant was measured at 492 nm (600 nm reference ...
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bioRxiv - Cancer Biology 2021Quote: ... were seeded in 96-wells white plate and caspase-1 activities were assessed using Caspase-Glo 1 Inflammasome Assay (Promega #G9951) according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2021Quote: Intracellular NanoBRET assays were performed as previously described (10) using NanoLuc®-STK3 Fusion Vector Cat.# NV4301 (Promega, Madison, WI, USA). Briefly ...
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bioRxiv - Cell Biology 2020Quote: ... Cell growth and viability were measured on the 96 well white bottom plate as an end point measurement for each time point and/or treatment using the Cell-titer GLO assay (Promega, # G7570) based on manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... TUNEL assay was performed using a DeadEnd(tm) Fluorometric TUNEL System according to the manufacturer’s instructions (Promega Corporation, Madison, WI, USA). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: ... The activities of firefly luciferase (LUC) and Renilla luciferase (REN) were measured using a Dual-Luciferase Reporter Assay System (E2920, Promega, USA). The LUC activity was normalized to the REN activity ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed with PBS and lysed directly on the plate on ice using the Cell Culture Lysis Reagent from the Luciferase Assay System (Promega#E1500). Lysates were transferred to microcentrifuge tubes ...
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bioRxiv - Developmental Biology 2020Quote: ... and 20 µL of resulting supernatant was used to measure luciferase activity with the Dual-Luciferase Reporter Assay System (Promega, E1980) on a GloMax-Multi plate reader (Promega).
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bioRxiv - Microbiology 2022Quote: ... medium was changed and cellular expression of luciferase reporter indicating viral infection was determined using ONE-Glo™ Luciferase Assay System (Promega) in 48 hr after infection ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cell viability was determined prior to gene expression studies on day 7 by measuring ATP release in supernatants with the CellTiter-Glo 3D assay (Promega, G9681) according to the manufacturer’s protocol to pre-specify appropriate testing ranges ...
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bioRxiv - Neuroscience 2021Quote: ... neonatal and adult astrocytes plated on 96-well plates (50,000 cells per well) was measured with the Glucose Uptake-Glo™ Assay (Promega, J1341) according to manufacturer’s protocol ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... The absorbance of cells was measured every 24 hours using CellTiter 96® Non-Radioactive Cell Proliferation Assay (Promega, Madison, WI) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 μL of cell lysate was distributed to mix with 25 μL of Nano-Glo Luciferase Assay System (Promega, Cat.# N1120), and another 25 μL of cell lysate was mixed with 25 μL of ONE-Glo Luciferase Assay System (Promega ...
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bioRxiv - Molecular Biology 2020Quote: ... and then Renilla luciferase activities were measured after the addition of 100 ml of Stop & Glo® Reagent (Luciferase Assay Reagent, Promega). Finally ...
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bioRxiv - Genomics 2020Quote: ... DNA was subcloned into the enhancer barrier vector28 (gift from Dr. Laura Elnitski, NHGRI) or pGL4.23 for enhancer assays (Promega, Madison, WI). Constructs were prepared using the Plasmid MidiPrep Kit (Qiagen ...
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured by adding 25 μL Luciferase Assay Buffer II (LARII) to 5 μL of lysate and measured immediately with the GloMax 20/20 Luminometer (Promega, E5311). Reactions were stopped and Renilla luciferase activity was measured by adding 25 μL Stop&Go reagent ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: PTC readthrough by ACE-tRNA was assessed by measuring NanoLuc (NLuc) luciferase activity using the Nano-Glo Luciferase Assay System (Promega, #N1130) on a Synergy2 multi-mode microplate reader (BioTek Instruments ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysates that produced over 104 luminescence units sing a high expression RNA containing NanoLuc Luciferase (A[WT]) and the Promega Nano-Glo Luciferase assay system (Promega N1110) in preliminary tests were pooled and optimized for the needed amount of Mg(OAc)2 and incubation time using A[WT] mRNA ...
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bioRxiv - Molecular Biology 2021Quote: ... The experiments performed here used a pool of lysates from multiple different4 harvests that were each individually tested for a minimal activity of 104 using a high expression RNA containing NanoLuc (A[WT]) and the Promega Nano-Glo Luciferase assay system (Promega N1110). Pooled lysates were then optimized for the needed amount of Mg(OAc)2 and an optimal incubation time at 37°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 8uL of reaction was mixed with 8uL of Nano-Glo buffer/substrate mix following the Nano-Glo Luciferase Assay System (Promega N1110) instructions ...
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bioRxiv - Developmental Biology 2019Quote: ... Cell lysates were harvested the day after and luciferase and β-galactosidase activities were measured using Luciferase Reporter and Beta-Glo Assay Systems (Promega) following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were incubated for 4 days and cell viabilty was measured using CellTiter-Glo® Luminescent Cell Viability Assay (Promega).
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bioRxiv - Physiology 2019Quote: ... Samples were mixed with the same amount of substrate provided by the One Glo Luciferase Assay System (Promega, Mannheim, Germany, #E6110) immediately before signal detection ...
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Characterization of GRK5 as a novel regulator of rhabdomyosarcoma tumor cell growth and self-renewalbioRxiv - Cancer Biology 2019Quote: RMS cell growth was assessed via cell counts or an ATP-based luminescent cell viability assay,CellTiter-Glo (Promega, Madison, WI). Myogenic differentiation was performed following serum starvation of RMS cells in 2% horse serum/DMEM for 72 hours prior to fixation in 2% paraformaldehyde ...
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bioRxiv - Cancer Biology 2019Quote: ... Cell cytotoxicity in the drug-treated 2D cell cultures was assessed using the CellTiter96® AQueous One Solution Cell Proliferation Assay (Promega). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... the cell viability of each infected virus variants was measured using CellTiter 96 Aqueous One solution Cell Proliferation Assay (Promega, USA). The relative cell viability was calculated by normalizing the absorbance value of treated virus samples against untreated virus samples ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Cell viability of LC5-RIC cultures exposed to HIV inoculum and test compounds was determined by performing a CellTiter-Blue® cell viability assay (Promega) and monitoring the ability of metabolically active cells to convert the redox dye resazurin into the fluorescent product resorufin ...
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bioRxiv - Molecular Biology 2020Quote: ... CA) already containing 20uL of room temperature 1X Passive Lysis Buffer from the Dual Luciferase Reporter Assay System (Promega, Madison, WI). Cultures were then lysed by shaking at 300 rpm for 25 minutes at room temperature ...
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bioRxiv - Genetics 2020Quote: ... Cells were harvested 48 hours after transfection and assayed for luciferase activity using the Dual-Luciferase Reporter (DLR™) Assay System (Promega). Data were expressed relative to renilla luciferase activity to normalize for transfection efficiency ...
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bioRxiv - Biophysics 2021Quote: ... and the twitchin kinase domain lacking flanking tails (Kin) was measured by monitoring ATP hydrolysis using the Kinase Glo™ luminescent assay system (Promega). Assays were performed using 10 µg of protein sample in a 50 µL reaction volume ...
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bioRxiv - Microbiology 2020Quote: ... Plates were incubated at 37°C (5% CO2) for 48 h before performing CellTiter-Glo (CTG) assays as per the manufacturer’s instruction (Promega, Madison, WI). Luminescence was read on a BioTek Synergy HTX plate reader (BioTek Instruments Inc. ...
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bioRxiv - Cancer Biology 2020Quote: ... expression of the luciferase reporter plasmid followed by measurement of luciferase activity after lysing cells with Bright-Glo Luciferase Assay System (Promega #E2610), both using Biotek Cytation 5 plate reader ...
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bioRxiv - Biochemistry 2020Quote: ... Cellular debris was then pelleted by centrifugation and either 5 μl or 10 μl of the clarified supernatant used in dual luciferase assays following the manufacturer’s prescribed protocol (Promega, cat # E1910). Data are shown as the ratio of RLUC to FLUC activity averaged over six experiments.
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bioRxiv - Neuroscience 2020Quote: 50 μl supernatant were collected as designed time point into a 96-well plate for lactate dehydrogenase (LDH) release assay as manufacture’s protocol (Promega, cat# G1780). Briefly ...