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Citations for Promega :
501 - 550 of 1665 citations for Precast Protein Gel since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and proteins were digested with LysC and trypsin (Promega) in two steps with a final enzyme-to-protein ratio of 1:25 ...
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bioRxiv - Genetics 2024Quote: ... A 1:10 ratio (enzyme: protein) of Trypsin (Promega) and LysC (Wako ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested using trypsin/ Lys-C mixture (Promega) and desalted on a 10 x 4.0 mm C18 cartridge (Restek ...
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bioRxiv - Cell Biology 2024Quote: ... containing trypsin (Promega; final 1/100 enzyme/protein ratio) and LysC (Wako ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were digested on-beads with Trypsin (Promega) with ratio enzyme:proteins 1:20 in 50 mM ammonium bicarbonate and performed overnight at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were then digested with 0.1 μg trypsin (Promega) at 37°C overnight ...
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bioRxiv - Microbiology 2023Quote: ... proteins were digested overnight with 0.63 µg trypsin (Promega). Tryptic digestion was stopped by adding formic acid to a final concentration of 1.8% (v/v) ...
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bioRxiv - Genetics 2023Quote: ... Eluted proteins were digested with sequencing-grade trypsin (Promega). Peptide mixtures were separated using Evosep One (Evosep Biosystems ...
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bioRxiv - Genetics 2021Quote: ... PCR products were purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and quantified with a Qubit 2.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... Gel pieces were rehydrated in a mixture of 4 ng/µL trypsin (Promega, Madison, WI) and 0.01% ProteaseMAX (Promega ...
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bioRxiv - Genomics 2020Quote: ... and in-gel digested overnight at 37°C with sequencing grade trypsin (Promega Cat# V5111). The peptides were then extracted with extraction buffer (50% acetonitrile (Fisher Chemical Cat#A955-4) ...
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bioRxiv - Cancer Biology 2020Quote: ... from the 1.5% TAE gel and cloned into the pGEM- T Easy Vector System (Promega) for transformation ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were then purified using Wizard SV Gel and PCR Clean-Up System (Promega) according to the instructions by the manufacturer ...
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bioRxiv - Cell Biology 2021Quote: ... The amplified product was purified using Wizard SV Gel and PCR Clean-up system (Promega) and inserted into T-Vector pMD20 (Takara Bio ...
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bioRxiv - Cancer Biology 2022Quote: ... PCR products were cleaned with the Wizard SV Gel and PCR Clean Up Kit (Promega) and cut with XhoI and BamHI or HindIII und BamHI (Thermo Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were purified by the Wizard SV Gel and PCR Clean-Up System (Promega) and quantified using the Qubit dsDNA HS assay (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Barcoded amplicons were purified by the Wizard SV Gel and PCR Clean-Up System (Promega) and quantified using the Qubit dsDNA HS assay ...
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bioRxiv - Biophysics 2022Quote: ... In-gel proteolytic digestion was performed overnight at 37°C using sequencing-grade trypsin (Promega). The resulting peptides were extracted at room temperature with 50% acetonitrile/0.1% trifluoroacetic acid ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were purified with Wizard® SV Gel and PCR Clean-Up System (Promega) and ligated to the pGEM-T easy® vector (Promega) ...
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bioRxiv - Microbiology 2021Quote: ... Amplicons were purified with the Wizard SV Gel and PCR Clean-Up system (Promega Switzerland).
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bioRxiv - Genetics 2022Quote: ... The digested minigenes were purified with Wizard SV Gel and PCR Clean-UP System (Promega) and ligated into a BamHI/MluI opened pCAS2 splicing reporter minigene vector (kindly provided by Dr ...
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bioRxiv - Molecular Biology 2019Quote: ... PCR products were purified using a Wizard SV Gel and PCR Clean-Up System (Promega) and forward and reverse sequences were obtained using an Abi 3130XL Genetic Analyzer (Applied Biosystem ...
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bioRxiv - Microbiology 2019Quote: ... Amplicons were purified using a Wizard SV Gel and PCR Clean up System kit (Promega) and EcoRI/XhoI digested pGREG533 was gel purified using a Qiaex II kit (Qiagen) ...
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bioRxiv - Biophysics 2019Quote: ... or the Wizard® SV Gel and PCR Clean-Up System (Promega GmbH, Mannheim, Germany). All primers were obtained from biomers.net ...
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bioRxiv - Cell Biology 2020Quote: ... PCR fragments were gel extracted and cloned with the pGEM-T easy Vector system (Promega) and transfected into XL10Gold competent cells (Agilent) ...
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bioRxiv - Microbiology 2019Quote: ... PCR products were purified using the Wizard SV Gel and PCR Clean-up System (Promega). The concentrations of amplified PCR products were measured using the Quant-iT PicoGreen dsDNA Assay Kit (Molecular Probes ...
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bioRxiv - Genomics 2020Quote: ... DNA quality and quantity was confirmed using gel electrophoresis and QuantiFluor dsDNA Sample kit (Promega) as per the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Gel bands were washed in 100mM AmBic/ACN prior to adding 1μg trypsin (Promega #V5111) for overnight incubation at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Resulting cDNAs were purified with the Wizard SV Gel and PCR Clean-Up System (Promega) and tailing with dATP and TdT ...
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bioRxiv - Microbiology 2022Quote: ... This DNA extract was then gel purified and quantified using a low-mass ladder (Promega). PCR was performed on ∼50 ng of DNA in a reaction mixture containing 1X Takara ExTaq PCR buffer ...
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bioRxiv - Plant Biology 2023Quote: ... and each slab was subjected to in-gel proteolytic digestion with trypsin (Promega, Mannheim, Germany) overnight as described in (Shevchenko et al. ...
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bioRxiv - Genomics 2022Quote: ... 1.6kb size amplicon was excised and purified using the SV gel clean up kit (Promega) and sent out for sequencing to confirm target ...
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bioRxiv - Cell Biology 2022Quote: ... The gel pieces were digested at 37°C initially with 0.5 µg of trypsin (Promega) per sample for 6 hours then additionally with 0.3 μg of trypsin overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... The PCR products were purified with Wizard SV Gel and PCR Clean-Up System (Promega). A volume of 0.75 µl of the DNA templates was mixed in 2.5 µl cell-free protein expression reactions (PUREFrex 2.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... Gel slices were pre-incubated with 20 μl of pre-activated trypsin (Promega France, Charbonnières) at room temperature for 15-20 min ...
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bioRxiv - Plant Biology 2023Quote: ... 5’RACE products were gel-purified and cloned in pGem®-T Easy vector (Promega). Independent clones were randomly chosen and sequenced by Sanger sequencing method.
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bioRxiv - Developmental Biology 2023Quote: ... then DNA was purified using the Wizard SV Gel and PCR Clean-Up System (Promega) according to manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Gel-purified PCR fragments were cloned into the pGEM-T Easy vector (Promega, cat# A137A) and sequenced using the Sanger’s method.
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bioRxiv - Evolutionary Biology 2023Quote: ... and purified with the Wizard SV Gel and PCR clean up System (Promega, Madison, USA). PCR products were cloned into pGem-T Easy vectors (Promega) ...
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bioRxiv - Biochemistry 2023Quote: ... the linearized DNA was purified using the Wizard® SV Gel PCR purification kit (Promega). For the synthesis of 5’-capped mRNA ...
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bioRxiv - Cancer Biology 2023Quote: ... PCR cleanup was conducted using the Wizard SV Gel and PCR Clean-Up System (Promega) with elution in 30 μl of double distilled water ...
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bioRxiv - Plant Biology 2023Quote: ... Products were cleaned with the Wizard® SV Gel and PCR Clean-Up System (Promega) and cloned with the pGEM®-T Vector Systems (Promega) ...
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bioRxiv - Microbiology 2023Quote: ... and the reaction was purified using Wizard SV Gel and PCR Clean-Up System (Promega). The linearized Donor was combined with the HCMV GW BAC in a Gateway reaction with LR Clonase II Plus enzyme (Thermo-Fisher ...
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bioRxiv - Microbiology 2023Quote: ... and purified using the Wizard SV Gel system and the PCR Clean-Up System (Promega).
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bioRxiv - Developmental Biology 2023Quote: ... The obtained products were gel purified and cloned into the pGEM-T Easy Vector (Promega). Individual clones were sequenced and analyzed.
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bioRxiv - Genetics 2023Quote: ... PCR products were purified using the Wizard SV Gel and PCR Clean-Up System (Promega) in line with the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... The resulting amplification product was gel purified and ligated to a pGEMT-easy (Promega, USA) vector ...
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bioRxiv - Cancer Biology 2024Quote: ... the DNA was purified using the Wizard SV Gel and PCR Clean Up Kit (Promega). Immunoprecipitated DNA was analyzed by qPCR using iTaq Universal SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... followed by gel purification of the 4725 bp band using a Wizard spin column (Promega). A multiple cloning site linker was prepared by annealing the primers oMJF055 and oMJF056 at 8 µM each in a buffer of 50 mM Tris HCl pH7.5 ...
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bioRxiv - Molecular Biology 2019Quote: The concentration and quality of proteins was assessed with a Bradford assay of the supernatant and confirmed with 12% SDS-PAGE.100 mM TEAB was used to dilute 100μg of protein solution with 8M urea that was subjected to digestion using Trypsin Gold (40:1: protein: enzyme, Promega, Madison, WI, USA) at 37°C overnight ...