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Citations for Promega :
501 - 550 of 961 citations for Octreotide acetate impurity C since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... Protein digestion was carried out overnight at 37°C with sequencing grade trypsin (Promega, Madison, WI, USA) at 1:40 (w/w ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were digested in-gel overnight at 37°C with Sequencing Grade Modified Trypsin (Promega; cat. #V5111) diluted in 200uL of digestion buffer (40 mM ammonium bicarbonate ...
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bioRxiv - Molecular Biology 2024Quote: ... The C terminus of rat Gβ1 was linked to peptide 86 subunit16,17 (also named as HiBiT, Promega) with a 15-amino acid polypeptide (GSSGGGGSGGGGSSG ...
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bioRxiv - Cell Biology 2020Quote: ... The sample was digested for 2 h with 0.5 µg of endoproteinase lysine-C (Wako chemicals) and then digested with 0.5 µg of trypsin (Promega) overnight ...
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bioRxiv - Genomics 2020Quote: ... Beads were resuspended in 50μL 100mM AMBIC and incubated overnight at 37°C with 400ng Trypsin (Promega V5280). Beads were supplemented with an additional 400ng Trypsin and incubated for a further 4 hours at 37°C ...
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bioRxiv - Biochemistry 2020Quote: ... Enzymatic digestion of was performed overnight at 37°C by the addition of sequencing grade trypsin (Promega, Wisconsin) (100 ng per sample ...
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bioRxiv - Cancer Biology 2021Quote: ... the protein extracts were then incubated overnight at 37°C with 20 μl of 25mM NH4HCO3 containing sequencing-grade trypsin (12.5μg/ml, Promega). The resulting peptides were desalted using ZipTip µ-C18 Pipette Tips (Millipore ...
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bioRxiv - Molecular Biology 2022Quote: ... at 72°C for 20min and then TA cloned into the pGEM-T Easy vector (Promega, Madison, WI). The cloned PCR products were Sanger Sequenced with M13 Forward (5’-TGTAAAACGACGGCCAGT-3’ ...
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bioRxiv - Biochemistry 2022Quote: ... followed by the secondary antibody for 1 h at +4 °C (anti-rabbit IgG-HRP, Promega 65-6120). The Pierce® enhanced chemiluminescence substrate (Thermo-Fischer Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... the beads with bound recombinant proteins were blocked for 1 h at 4°C using reticulocyte lysate (Promega). Next ...
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bioRxiv - Microbiology 2020Quote: ... emission 410 nm) was immediately measured every 15 minutes at 37°C using a GloMax plate reader (Promega).
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bioRxiv - Neuroscience 2020Quote: ... The mixture was then and incubated for 7min at 55°C followed by an additional 7 minute incubation with 3.5µl of SDS 0.2% (Promega, #V6551) to ensure that Tn5 was dissociated from the cDNA ...
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bioRxiv - Molecular Biology 2022Quote: ... the proteins were digested overnight at 37 °C with 6 ng μL-1 of Asp-N (Promega, UK). Peptides were extracted in 2 % v/v formic acid ...
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bioRxiv - Microbiology 2022Quote: ... Proteins on magnetic beads were digested overnight at 37°C with 1μl (0.2 μg/μL) of trypsin (Promega) in a 25-mM NH4HCO3 buffer per sample ...
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bioRxiv - Cell Biology 2020Quote: ... The proteins were then digested overnight at 37°C with 0.2 μg of modified sequencing grade trypsin (Promega) in 50 mM ammonium bicarbonate ...
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bioRxiv - Cell Biology 2021Quote: ... the samples were diluted to 1.5 M urea using 20 mM HEPES (pH 8.5) and digested at 30°C overnight with trypsin (Promega) at a ratio of 70:1 (w/w) ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were digested on the column with 0.25 µg of Trypsin/Lys-C protease mix (Promega; cat. V5071), and peptides eluted for mass spectrometry.
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bioRxiv - Cancer Biology 2021Quote: ... and sequentially digested with Lys-C (Wako) (protein:enzyme ratio 1:50, o/n at RT) and trypsin (Promega) (protein:enzyme ratio 1:50 ...
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bioRxiv - Developmental Biology 2021Quote: ... into 100 mM ammonium bicarbonate and digested overnight at 37 °C with mass spectrometry grade trypsin gold (Promega) at a 1:50 trypsin:protein ratio ...
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bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
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bioRxiv - Microbiology 2020Quote: ... where the samples were destained and subjected to in-gel proteolytic digestion with trypsin/Lys-C mixture (Promega). The digests were extracted ...
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bioRxiv - Microbiology 2022Quote: ... The concentrated phages were next treated for 30 min at 37°C with 10 μL of DNAse (Promega) and 2.5μL of RNAse (Macherey-Nagel ...
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bioRxiv - Biophysics 2022Quote: ... and the two halves were digested with either Sequencing Grade Modified Trypsin or endoproteinase Glu-C (both Promega, Madison ...
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bioRxiv - Molecular Biology 2023Quote: ... Enzymatic digestion was performed at 37°C and overnight with 500 ng trypsin (Promega, Charbonnieres les Bains, France). Peptide mixtures were then desalted on C18 spin-column and dried in a Speed-Vacuum.
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bioRxiv - Molecular Biology 2023Quote: ... Ligation of the digested pGEM-T easy vector and 5’ fragments was carried out overnight at 4 °C using 0.5 µl (1.5 U) of T4 DNA Ligase (Promega). pGEM-T easy vector containing the 5’ fragment (5’-pGEM-T ...
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bioRxiv - Neuroscience 2024Quote: ... Prior to overnight digestion at 37 °C using modified porcine trypsin (Mass Spectrometry Grade, Promega, Madison, WI, USA) at an enzyme–protein ratio of 1:80 ...
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bioRxiv - Biochemistry 2023Quote: ... then reverse transcribed for 1 hr at 37 °C using M-MLV reverse transcriptase (Promega, Madison, WI; M1705) in the presence of RNase inhibitor (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... were digested overnight at 37 °C using trypsin with a 1:20 trypsin to sample ratio (Promega, #V5117). The next day ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were further processed by digesting them overnight with a Trypsin/Lys-C mix (Promega, Madison, USA) at a 50:1 substrate:enzyme ratio (w/w ...
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bioRxiv - Microbiology 2023Quote: ... The concentrated phages were next treated for 30 min at 37°C with 10 µl of DNAse (Promega) and 2.5 µl of RNAse (Macherey-Nagel ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were digested in 100 mM ammonium bicarbonate containing 2 μg of trypsin/Lys-C enzyme mix (Promega) at 37 °C overnight ...
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bioRxiv - Physiology 2023Quote: ... Samples were incubated for 60 minutes at 47°C with sequencing grade trypsin (Promega, San Luis Obispo, CA) dissolved in 50 mM TEAB at a 1:25 (w/w ...
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bioRxiv - Molecular Biology 2023Quote: Reduced and alkylated proteins were prepared by in-gel digesting overnight at 37°C using 100ng trypsin (Promega). The supernatant was dried by vacuum centrifugation and the samples were resuspended in 0.1% trifluoro acetic acid ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in 0.1M TEAB and overnight (∼16 h) digestion with trypsin/Lys-C mix (1:25 protease:protein; Promega) at 37° C was performed with constant mixing using a thermomixer ...
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bioRxiv - Microbiology 2023Quote: ... Trypsin digest was allowed overnight at 37°C by the addition of 1 μg of trypsin (#V5111, Promega) to each sample ...
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bioRxiv - Immunology 2023Quote: ... and then subjected to overnight digestion at 37°C with sequencing-grade modified trypsin (Promega, Madison, WI, USA). The peptides were extracted ...
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bioRxiv - Plant Biology 2023Quote: ... Luciferase activity was then measured at room temperature (below 25 °C) using the Luciferase Assay System (Promega Corporation) in a Glomax luminometer (Promega Corporation ...
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bioRxiv - Neuroscience 2023Quote: ... 2018) resulting in two fractions after sequential proteolysis with first Lys-C (Wako Chemicals) followed by trypsin (Promega). Peptides were analyzed on a Q Exactive HF mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... and digested according to the manafacturer’s protocol for 1 hour at 50 °C by Trypsin Platinum (Promega, WV), digested material was dried in speedvac (Eppendorf ...
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bioRxiv - Cell Biology 2023Quote: ... The digestion was performed in a 50 mM TEAB buffer with 0.2 μg of Lys-C (Wako Chemicals) and 0.4 μg of trypsin (Promega). Trifluoroacetic acid (TFA ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then subjected to digestion with LysC (Wako) at 37°C for 2 hours and trypsin (Promega) overnight at 37°C at a 1:50 enzyme:protein ratio ...
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bioRxiv - Microbiology 2024Quote: ... Then they were diluted again to ¼ and digested overnight at 37 °C with sequencing grade-modified trypsin (Promega) at a ratio of 1:50 ...
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bioRxiv - Microbiology 2024Quote: ... and then incubated for an additional hour at 55°C after adding 20 mg/ml proteinase K (Promega). DNA was extracted using the ZymoBIOMICS DNA Miniprep Kit (Zymo Research) ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were then digested into peptides by the addition of 1:1000 (g/g) Lys-C Protease (Promega) for 4 h at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... All samples were then digested overnight at 37°C with using 500 ng sequencing grade modified trypsin (Promega).
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bioRxiv - Developmental Biology 2024Quote: ... the samples were stored at −80°C according to the protocol of ReliaPrepTM RNA Tissue Miniprep System (Promega). cDNA was synthesized from 400 ng of total RNA using PrimeScript RT Master Mix (Perfect Real Time ...
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bioRxiv - Microbiology 2020Quote: ... Urea was reduced to 1 M by addition of 500 μl AB and overnight digestion was performed at 37 °C with 5 μl of freshly prepared 0.2 μg/μl trypsin (Promega) in AB ...
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bioRxiv - Synthetic Biology 2021Quote: ... pCR-U6-gRNA-miniCMV-Nluc was generated by replacing TdTomato with Nluc from pNLF1-C [CMV/Hygro] (Promega #N1361) using Gibson Assembly ...
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bioRxiv - Microbiology 2020Quote: ... ratio 50:1 (protein:rLysC) for 3h/37°C and then digested with Sequencing Grade Modified Trypsin (#V5111, Promega, France) ratio 50:1 (protein:trypsin ...
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bioRxiv - Cell Biology 2020Quote: ... the gel pieces were covered with 200 μl of 20 ng/μl of sequence grade trypsin/Lys-C (Promega) in 50 mM ammonium bicarbonate pH8 buffer ...