Labshake search
Citations for Promega :
4951 - 5000 of 5637 citations for hsa mir 185 Real time RT PCR Detection Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2019Quote: Genomic DNA was isolated from the tip of tails of 3-week-old mice using Wizard Genomic DNA purification Kit (Promega), amplified by PCR ...
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bioRxiv - Microbiology 2019Quote: The pBESTluc plasmid was used as a backbone for all reporter assay constructs and was included in the Luciferase Assay System kit (Promega). pBEST + metK was created by inserting nucleotides corresponding to the first 10 codons of the C ...
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bioRxiv - Microbiology 2019Quote: ... Lifted cells were resuspended with an additional 50 µl of DMEM before adding to 50 µl Nuclei Lysis Buffer (Wizard Genomic DNA Purification kit; Promega) and frozen at −20°C overnight ...
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Global genetic patterns reveal host tropism versus cross-taxon transmission of bat BetacoronavirusesbioRxiv - Evolutionary Biology 2020Quote: Genomic RNA was extracted from small intestine and large intestine samples using the SV Total RNA Isolation kit (Promega, USA) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2020Quote: ... and from the interior of the sponge tissue (<2mm from the exterior pinacoderm) and the seawater filter papers using the Wizard Genomic DNA Purification Kit (Promega).
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bioRxiv - Plant Biology 2020Quote: ... only root parts (around 100 mg) were collected and total RNA was extracted using a Trizol-adapted ReliaPrep RNA Tissue Miniprep Kit (Promega). For verifying the transcript level in single T-DNA lines ...
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bioRxiv - Genetics 2019Quote: In vitro transcription and translation were realized from 500ng of cDNA pGEM-T construct using the TnT T7 Quick Coupled Transcription/Translation kit (Promega) and Transcend Biotin-Lysine-tRNA following the manufacturer protocol ...
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bioRxiv - Genetics 2021Quote: ... The genomic DNA (gDNA) of individual transient G0 mosquitoes was extracted using the Wizard® Genomic DNA Purification Kit (Promega). In the T7 Endonuclease I (T7EI ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 4 μg of the extracted material was then used as template to in vitro synthesized viral genomic RNA transcripts using the Ribomax T7 RNA transcription Kit (Promega) and Ribo m7G Cap Analogue (Promega ...
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bioRxiv - Cell Biology 2021Quote: ... On day eight representative photographs were taken using an EVOS XL microscope and viability was assayed using the CellTiter-Glo Luminescent Cell Viability kit (Promega) per the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Cell death was determined according to the activity of lactate dehydrogenase (LDH) in the culture supernatants using a CytoTox1 Kit according to the manufacturer’s instructions (Promega, USA).
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bioRxiv - Bioengineering 2020Quote: ... lysozyme secretion and cell viability were assessed using Lysozyme Assay Kit (EnzChek) and CellTiter-Glo 3D (CTG 3D) Cell Viability Assay (Promega), respectively ...
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bioRxiv - Neuroscience 2020Quote: Caspase 3/7 activity in primary neuronal cells was measured using ApoTox-Glo Triplex Assay kit (Promega, Madison, WI, USA) as per the manufacture’s instruction ...
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bioRxiv - Microbiology 2021Quote: ... The luciferase expression levels were measured 24h post-transfection using a Nano-Glo Dual-Luciferase Reporter Assay kit (Catalogue number N1610, Promega) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2021Quote: Caspase 3/7 and 9 activities were assessed using a fluorescence-based Apo-ONE homogenous caspase 3/7 assay kit (Promega) and luminescence-based caspase-glo 9 assay system (Promega) ...
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bioRxiv - Cancer Biology 2020Quote: ... The viability of cells was measured after 3 days incubation using a CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2020Quote: ... grown for 48 h and then caspase 3/7 enzymatic activity determined using Apo-One Homogenous caspase 3/7 activity assay kit (Promega) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Luciferase activity was tested 24 hours after plasmid transfections using the Dual-Glo Luciferase Assay System kit (Cat# E2920, Promega) as per the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Nucleic acids deriving from PCRs or restriction digests reactions were purified from 1% TAE agarose gels with the “Wizard SV Gel and Purification System Kit” (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted from nasopharyngeal swab samples using Maxwell® 16 Viral Total Nucleic Acid Purification Kit (Promega, Wisconsin-USA) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2021Quote: All NanoLuc luciferase assays to quantify gene expression were performed with cell lysate according to the procedure described in the Nano-Glo Luciferase Assay System kit (Promega). For these experiments ...
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bioRxiv - Microbiology 2020Quote: A random-primers based reverse transcription of 1 μg of RNA was performed with the A3500 Reverse Transcription System Kit (Promega), followed by quantitative real-time PCR on cDNA using the FastStart Essential DNA Green Master kit (Roche ...
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bioRxiv - Plant Biology 2020Quote: ... were collected from each genotype and total RNA was extracted using a Trizol-adapted ReliaPrep RNA Tissue Miniprep Kit (Promega). Reverse transcription was carried out with PrimeScript RT Master Mix (Takara) ...
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bioRxiv - Plant Biology 2021Quote: ... ground in liquid nitrogen and total RNA from approximately 150 mg of ground leaf material was extracted with a ReliaPrep miRNA Cell and Tissue Miniprep System kit (Promega). A stem loop pulsed RT-PCR protocol [8 ...
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bioRxiv - Neuroscience 2020Quote: ... Cell viability was also determined based on lactate dehydrogenase (LDH) activity in conditioned culture media using a commercial kit (G1780, Promega) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Cell viability was assessed 3 days postinfection by MTS assay using a Cell Titer 96 aqueous cell proliferation kit (Promega) or Viral ToxGlo™ (ToxGlo assay) ...
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bioRxiv - Microbiology 2021Quote: High molecular weight DNA was extracted using the Maxwell® RSC PureFood GMO and Authentication Kit as for qPCR (Promega). The high molecular weight DNA was sent to the Functional Genomic Center Zurich (FGCZ ...
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bioRxiv - Microbiology 2021Quote: ... Approximately 4 μg of the extracted material was then used as template to in vitro synthesized viral genomic RNA transcripts using the Ribomax T7 RNA transcription Kit (Promega) and Ribo m7G Cap Analogue (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was also extracted from inactivated grown cultures using a DNA extraction kit according to the manufacturer’s instructions (Promega, USA).
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bioRxiv - Microbiology 2021Quote: Epithelial cytotoxicity was determined by quantification of lactate dehydrogenase activity in A549 culture supernatants at 24 h post fungal challenge using the Cytox 96 Non-Radioactive Cytotoxicity Assay kit (Promega) according to the manufacturer’s protocol and using recombinant porcine LDH enzyme (Sigma-Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... MT4 and TMZR5 cells were transduced with the genes in Fig 2G as before and 96 hours post-transduction the supernatants were harvested to measure toxicity of the expressed ISGs using the CytoTox-Glow kit (Promega). In a separate experiment ...
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bioRxiv - Molecular Biology 2020Quote: For the analysis of cell vitality of Calu-3 cells treated with camostat mesylate or FOY-251 the CellTiter-Glo Luminescent Cell Viability Assay kit (Promega) was used ...
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bioRxiv - Molecular Biology 2020Quote: ... phaffii cells were cultured for 12-14 h and total RNA was isolated using RNA isolation kit (Cat. # Z3100, Promega) as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: gDNA was extracted from stationary phase evolved cocultures following revival from frozen stocks using a Wizard Genomic DNA purification Kit (Promega). DNA fragment libraries were constructed for samples from shaking WT-based cocultures A-F and NifA*-based cocultures M-R at generation ∼146 using NextFlex Bioo Rapid DNA kit ...
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bioRxiv - Microbiology 2019Quote: ... the aptamer pool from the 10th round of SELEX was cloned with the pGEMT-easy Cloning and Ligation Kit (Promega). To investigate the effect of counter-selection on the aptamer pools ...
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bioRxiv - Microbiology 2019Quote: Genomic DNA was extracted from loopfuls of pure MTBC colonies grown on Lowenstein-Jensen media (37) using the Maxwell 16 DNA Purification Kit (Promega). DNA from Nigerian strains was extracted using the Cetyl trimethylammonium bromide (CTAB ...
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bioRxiv - Cancer Biology 2020Quote: ... Caspase3/7 activity was measured 3 days after the treatment with a commercial available kit (Caspase-Glo 3/7 Assay, Promega).
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bioRxiv - Cancer Biology 2020Quote: ... Cells were seeded in 96 wells plate and luciferase activities were measured using Dual-Luciferase Reporter Assay System kit as described by the manufacture (Promega). Fluc/Rluc ratios were determined as relative luciferase activities.
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bioRxiv - Cell Biology 2021Quote: ... genomic DNA from Proteinase K-digested mouse toes was extracted using the Wizard SV genomic DNA extraction kit (Promega, A2360). PCR reactions to amplify a fragment containing the target sites were carried out using the forward and reverse primers (Forward primer ...
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bioRxiv - Biophysics 2020Quote: ... The cell viability at the same wells was determined by using CellTiter-Glo Luminescent Cell Viability Assay Kit (# G7571, Promega). Neutralization efficiency was calculated as relative fluorescence to the conditioned media collected from mock-transfected cells.
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bioRxiv - Cell Biology 2021Quote: ... The RNA concentrations were measured and 1ug RNAs were used to synthesize cDNA using the GoScript Reverse Transcription System kit(Promega).
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bioRxiv - Cancer Biology 2021Quote: ... luciferase assay reagent was mixed in a 1:1 ratio with cell lysate (Luciferase Assay System kit, Promega, Madison, WI). Luciferase activity was measured with Synergy H4 Hybrid Reader (BioTek ...
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bioRxiv - Cancer Biology 2021Quote: ... cells using ITX2 transfection reagent and luciferase activity (renilla and firefly) was measured 24hours later with the Dual Glow-Stop and Glow luciferase kit (Promega) using a BioTek luminometer (Synergy).
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... PCR products were isolated with a QIAquick Gel Extraction kit (Quiagen) and purified fragments cloned into pGEM-T Easy vector (Promega). Specific details for the generated probes are as follow (gene name (accession number) ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were loaded with 5 μM CellROX in media for 30 min and loaded and unloaded control cells analysed for mCherry channel signal using flow cytometry as described above GSH and GSSG levels were measured using GSH/GSSG-Glo assay kit from Promega according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... levels of Firefly and Renilla luminescence in cell lysates were measured by Synergy H1 microplate reader according to Promega’s Dual-Glo kit protocol (Promega #E2920). Fold changes of AP-1 activity were presented by the ratio of normalized Firefly/Renilla to control wells.
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bioRxiv - Molecular Biology 2022Quote: ... The SELENOP 3’UTR and the control plasmid were linearized using Bsb 1 and in vitro transcribed using the Ribomax kit (Promega). The RNA was then purified using p30 size exclusion columns (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... DNA extractions were carried out using the Wizard® Genomic DNA Purification Kit (Promega, Madison, WI, United States of America) following Lehner et al ...
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bioRxiv - Plant Biology 2022Quote: ... Samples representing different stages of the parasitizing process (i.e. root development) were extracted using the Maxwell® RSC Plant RNA kit (Promega) with the Maxwell® RSC Instrument (Promega) ...
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bioRxiv - Microbiology 2022Quote: ... Luciferase activity (Relative Luciferase Units or RLU) was detected at 72h post-infection by using Bright-Glo Luciferase Assay System Kit (Promega) in a Microplate Luminometer (Wallac-Perkin Elmer).