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Citations for Promega :
451 - 500 of 683 citations for TNF Receptor II Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and 1 μg of RNA used as a template for cDNA synthesis with random primers and ImProm-II reverse transcriptase (Promega).
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bioRxiv - Plant Biology 2023Quote: ... Five micrograms of total RNA were ligated to the RNA GeneRacer oligo adapter and subjected to reverse transcription utilizing Improm-II Reverse Transcriptase kit (Promega). The cDNA was used for amplification of cleaved PYL6 fragments ...
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bioRxiv - Cancer Biology 2023Quote: ... The Genetic Resources Core Facility at the John Hopkins University School of Medicine authenticated the HPAF-II cells by performing short tandem repeat profiling via GenePrint 10 (Promega) and comparing to the ATCC database ...
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bioRxiv - Evolutionary Biology 2023Quote: ... the bands were excised from the gel and TA cloned using the pGEM- T easy-vector II cloning kit (Promega). The standard mini-prep step was avoided by using colony PCR ...
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bioRxiv - Microbiology 2023Quote: ... Firefly luciferase activity due to viral polymerase activity and Renilla luciferase activity due to RNAP II activity were measured using the Dual-Glo Luciferase Assay system (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... and 1 μg of RNA used as a template for cDNA synthesis with random primers and ImProm-II reverse transcriptase (Promega). Quantitative PCR (qPCR ...
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bioRxiv - Cell Biology 2019Quote: ... Cell signalling) or rabbit anti-TgTOM40 (1:2000, (van Dooren et al., 2016)) antibodies coupled to secondary horseradish peroxidase (HRP)(Promega for mouse and rabbit, Abcam for rat) conjugated antibodies (1:10,000 ...
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bioRxiv - Genetics 2021Quote: ... Approximately 1 μg RNA per sample was converted to cDNA using the ImProm-II Reverse Transcription System kit (Promega, Madison, WI) using the Oligo(dT)15 primer supplied with the kit and with a final concentration of 3 mM MgCl2 according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The larger of either 1μg of RNA or 5μL of RNA were used as a template for cDNA synthesis by the ImProm-II reverse transcriptase system (Promega, Madison, WI) after DNA contamination was removed with the DNAfree kit (Life Technologies) ...
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bioRxiv - Molecular Biology 2021Quote: ... Firefly luciferase activity was measured by adding 25 μL Luciferase Assay Buffer II (LARII) to 5 μL of lysate and measured immediately with the GloMax 20/20 Luminometer (Promega, E5311). Reactions were stopped and Renilla luciferase activity was measured by adding 25 μL Stop&Go reagent ...
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bioRxiv - Neuroscience 2021Quote: ... First strand cDNA was synthesized in 20 µl reaction using oligo-dt primers and ImProm-II Reverse Transcriptase (Promega, Milan, Italy) with 5 µl of RNA according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... 3 μg of total RNA was incubated with 1mM of Oligo-AP primer (Supplementary Table I) and Improm II reverse transcriptase (Promega®) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2021Quote: ... was synthesized from 1 μg of total RNA, using oligo-dT primers (USB Corporation, Cleveland, USA) and a reverse transcriptase kit (ImPROm-II, Promega, USA), according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: Total RNA was extracted from Hydra polyps starved for 48 hrs and cDNA was synthesized from total RNA using Improm-II reverse transcriptase system (Promega™) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... purified total RNA was reverse transcribed into cDNA by ImProm-II™ Reverse Transcription System (Promega; Madison, WI; Cat. No. A3800). Target gene fragments were first amplified from a mixed cDNA library of planula larva and primary polyps ...
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bioRxiv - Neuroscience 2022Quote: ... First strand of cDNA was synthesized from 0.5-1 μg of total RNA using Im-Prom-II system (Promega, Cat. A3800). Real-Time PCR was performed using iTaq qPCR master mix according to manufacturer’s instructions (Bio-Rad ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA (1 μg) was reverse-transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, Fitchburg, WI, USA).
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bioRxiv - Microbiology 2023Quote: ... of virus pool stock was used for viral RNA extraction by GeneAid viral RNA extraction kit (GeneAid) and converted to cDNA using ImProm-II™ reverse transcription system (Promega) according to manufacturers’ protocols ...
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bioRxiv - Plant Biology 2022Quote: ... Total RNA (1 μg) was reverse-transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, Fitchburg, WI, USA).
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bioRxiv - Cell Biology 2019Quote: ... Translations were performed using the Flexi® Rabbit Reticulocyte Lysate System (Promega) supplemented with DTT (10 mM ...
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bioRxiv - Microbiology 2020Quote: ... Immune complexes were detected with anti-rabbit peroxidase-conjugated secondary antibodies (Promega) and enhanced chemiluminescence reagents (Pierce ...
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bioRxiv - Neuroscience 2021Quote: mRNAs were in vitro translated with Flexi Rabbit Reticulocyte Lysate System (Promega), as previously described (23 ...
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bioRxiv - Molecular Biology 2021Quote: ... 200 μL of rabbit polyclonal antibody against PLRV-CP (1:5000; Promega) was added to each well and incubated for 3 h at 37 °C ...
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bioRxiv - Biochemistry 2019Quote: Translations were performed using the Flexi® Rabbit Reticulocyte Lysate System (Promega) supplemented with 10 mM DTT (Melford ...
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bioRxiv - Immunology 2019Quote: ... HRP-conjugated antibodies (anti-mouse or anti-rabbit IgG HRP conjugate, Promega) were detected by enhanced chemiluminescence detections (Thermofisher ...
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bioRxiv - Biochemistry 2020Quote: ... Lep variants were then translated using rabbit reticulocyte lysate (Promega, Madison, WI) supplemented with canine rough microsomes (tRNA probes ...
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bioRxiv - Molecular Biology 2021Quote: In vitro translation in RNase-treated Rabbit Reticulocyte Lysate System (RRL, Promega) was carried out as recommended by the manufacturer with slight modifications ...
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bioRxiv - Neuroscience 2020Quote: ... or anti-rabbit IgG pAb conjugated alkaline phosphatase (1:10,000, S3731, Promega) were used as secondary antibodies and visualized by detecting chemiluminescence (32209 ...
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bioRxiv - Neuroscience 2022Quote: ... and anti-activated Caspase 3 (rabbit, Promega, catalog #G7481, 1:250, RRID:AB_430875), and appropriate secondary antibodies ...
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bioRxiv - Molecular Biology 2019Quote: ... reactions were prepared as per Rabbit Reticulocyte Lysate System (Promega Cat#L4960). 10 µL of reaction mixture was supplemented with 1 µg of either Renilla or Firefly luciferase mRNA alongside recombinant GST or GST-PKM1 ...
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bioRxiv - Molecular Biology 2021Quote: Messenger RNAs were translated in nuclease-treated rabbit reticulocyte lysate (RRL) (Promega). Typical reactions were composed of 90% (v/v ...
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bioRxiv - Cell Biology 2022Quote: ... Goat immunoglobulins against rabbit or mouse immunoglobulins conjugated with horseradish peroxidase (Promega) were used as secondary antibodies ...
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bioRxiv - Microbiology 2023Quote: ... followed by horseradish peroxidase-conjugated (HRP) secondary rabbit antibodies (Promega, 1/25000) and revealed by chemiluminescence (Pierce ...
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bioRxiv - Cell Biology 2022Quote: ... HRP conjugated anti-rabbit (W4011) and anti-mouse (W4021) were from Promega and used at a dilution of 1:10000 ...
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bioRxiv - Genetics 2023Quote: ... Secondary horseradish peroxidase-conjugated anti-mouse or anti-rabbit IgG antibodies (Promega) were used at 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... secondary antibodies: anti-rabbit HRP or anti-mouse HRP (Promega, 1:10000).
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibodies used were: anti-rabbit-IgG-HRP (W401B, 1:8000, Promega); anti-mouse-IgG-HRP (W402B ...
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bioRxiv - Molecular Biology 2019Quote: ... Two micrograms of total RNA were reverse transcribed into the first strand cDNA using the ImProm-II Reverse Transcription System (A3800, Promega, Madison, USA) according to the manufacturer’s protocol and used as a template for the amplification of the full-length camel IFNε cDNA ...
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bioRxiv - Synthetic Biology 2019Quote: ... The RNA was treated with Promega RQ1 DNase and reverse transcribed into cDNA using the Improm-II Reverse Transcription kit (Promega; Fitchberg, WI). Steady-state transcript levels were then measured using the cDNA template in quantitative PCR (qPCR ...
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bioRxiv - Neuroscience 2021Quote: ... One microgram of total RNA was transcribed to cDNA by reverse transcriptase enzyme action Improm Pre-II ® (Promega, Madison, Wisconsin, USA). Quantitative RT-PCR reaction in real time was done on an ABI Prism ® 7500 Sequence Detection System (Applied Biosystems) ...
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bioRxiv - Cell Biology 2022Quote: ... DNase-treated RNA was transcribed into complementary DNA (cDNA) using the ImProm-II™ reverse transcription system and oligo-dT as primer (Promega, Germany). Quantitative real-time reverse transcriptase polymerase chain reaction (qPCR ...
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bioRxiv - Genomics 2020Quote: Genomic DNA was extracted from whole blood obtained from GALA II study subjects using the Wizard Genomic DNA Purification kits (Promega, Fitchburg, WI), and DNA was quantified by fluorescent assay ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was synthesized with primers specific for each strand separately at 50 °C (Supplemental Table S1) using the ImProm-II™ Reverse Transcription System (Promega, A3800) and subjected to qPCR with primers specific for the viral N-protein (Supplemental Table S1).
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bioRxiv - Neuroscience 2023Quote: ... 1ug of total RNA extracted from transfected SK-N-MC cells was reverse transcribed using the ImProm-II Reverse Transcription System and random hexamer primers (Promega, Madison, MI). PowerUp SYBR™ Green Master Mix (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed using 1 µg of total RNA in combination with oligo(dT) and Improm-II reverse transcriptase (Promega, Madison, WI). qPCR analysis was performed as described previously [55] with SYBR green PCR master mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Radiolabelled protein was translated from mRNA using the rabbit reticulocyte lysate system (Promega) and 35S-labelled methionine ...
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bioRxiv - Molecular Biology 2019Quote: ... In vitro translation reactions were performed in nuclease-treated Rabbit Reticulocyte Lysate (Promega) as described by the manufacturer ...
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bioRxiv - Microbiology 2019Quote: ... Secondary antibodies (anti-mouse or anti-rabbit) conjugated to horseradish peroxidase (HRP, Promega) were diluted 1:25,000 in 3% (w/v ...
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bioRxiv - Microbiology 2021Quote: ... Immunodetection was carried out using rabbit polyclonal anti-Halo antibody (Promega; 1:1000), mouse anti-HA antibody (Roche ...
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bioRxiv - Microbiology 2022Quote: ... followed by a horseradish peroxidase (HRP)-conjugated anti-rabbit IgG secondary antibody (Promega). Chemiluminescence signals were detected using the GE ImageQuant LAS4000 camera (GE Healthcare Life Sciences).