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Citations for Promega :
451 - 500 of 663 citations for Strat M Membrane Transdermal Diffusion Test Model since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Protein digestion was carried out in digestion solution (5 ng trypsin/μl in 50 mM ABC, 10% ACN, 0.01% (m/v) ProteaseMAX surfactant (Promega), 1 mM CaCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... the solution was diluted to 2 M urea by the addition of 20 mM HEPES pH 8.0 and digested with sequencing-grade modified trypsin (Promega) at a final concentration of 5 µg/ml overnight on RT ...
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bioRxiv - Immunology 2020Quote: LDH release was measured using the CytoTox One homogenous membrane integrity assay (Promega). Cytotoxicity was calculated as a function of LDH release using the following formula ...
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bioRxiv - Neuroscience 2020Quote: ... The membranes were probed with horseradish peroxidase-conjugated secondary IgG (1:7000, Promega) for 1 hr at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... and cytotoxicity assayed using CytoTox-ONE™ Homogeneous Membrane Integrity Assay Kit (Promega). For HL-1 infected cardiomyocytes ...
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bioRxiv - Neuroscience 2023Quote: ... on-membrane DNase I digestion was performed according to the manufacturer’s protocol (Promega). The integrity of the total RNA was assessed using spectrophotometric analysis using NanoDrop 2000/2000c ...
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bioRxiv - Microbiology 2024Quote: ... the membranes were incubated with horseradish peroxidase-conjugated anti-mouse IgG (Promega, Japan) diluted 1:10,000 in 1× TBST ...
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bioRxiv - Molecular Biology 2024Quote: ... the membrane was probed with a horseradish peroxidase-conjugated mouse secondary antibody (Promega). The image was visualized with SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo fisher Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... The RNA (50-250 ng) was reverse-transcribed with the Moloney Murine Leukemia Virus reverse transcriptase (M-MLVRT) (Promega, M368B) and amplified using the iTaqTM Universal SYBR® Green Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... then 1 µg RNA was used to synthesize the oligo(dT) primed first-strand cDNA using the M-MLV reverse transcriptase cDNA synthesis kit (Promega). Quantitative RT-PCR was performed using a CFX96 Real-Time PCR detection system (Bio-Rad ...
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bioRxiv - Molecular Biology 2020Quote: ... Equal amounts of RNA (1 μg) were reverse-transcribed with a mixture of oligo(dT)15 and random hexamer primers using M-MLV polymerase (Promega). RT-qPCR gene expression quantifications were performed using AceQ qPCR SYBR Green Mix (Vazyme Biotech ...
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bioRxiv - Cell Biology 2022Quote: ... two micrograms of RNA from each sample were reverse transcribed at 42 °C for 1 h followed by 95 °C for 10 min M-MLV Reverse Transcriptase (Promega) and oligo(T)18 primers (Takara) ...
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bioRxiv - Molecular Biology 2021Quote: ... of RNA were reverse-transcribed into single-stranded complementary cDNA using an oligo-dT primer and M-MLV Reverse Transcriptase (Promega). The single-stranded cDNA products were amplified by PCR using gene-specific sense and antisense primers (mRNA TPK1 ...
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bioRxiv - Plant Biology 2020Quote: ... Reverse transcription was achieved with 4 µg of RNAs in the presence of Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase (RNase H minus, Point Mutant, Promega) after annealing with an anchored oligo(dT)18 primer as described (Wirth et al. ...
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bioRxiv - Pathology 2020Quote: ... Sample pH was adjusted to 7.5 with 0.05 M TEAB and proteins were digested overnight at 37 °C with sequencing-grade trypsin (Promega, Madison, WI) in 1:30 (w/w ...
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bioRxiv - Immunology 2021Quote: ... and cDNA was synthesized via reverse-transcription of 1 μg of RNA using M-MLV Reverse Transcriptase (Promega (Wisconsin, USA). cDNA was used to analyse differences in gene expression by qRT-PCR employing iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2020Quote: 1μg of total RNA from the human and mouse brain specimen was reverse-transcribed using the M-MLV reverse transcriptase (Promega) for efficient synthesis of the first-strand cDNA ...
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bioRxiv - Plant Biology 2021Quote: ... 1 μg of RNA was converted into cDNA with M-MLV RNase H–Point Mutant reverse transcriptase (Promega Corp., USA) and an anchored oligo dT21 primer (Metabion ...
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bioRxiv - Plant Biology 2020Quote: ... One μg of total RNA was used for first-strand cDNA synthesis with an oligo(dT) primer and M-MLV reverse transcriptase (Promega). The primer sequences are listed in Supplementary Table S1 ...
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bioRxiv - Plant Biology 2020Quote: ... and then reverse-transcribed with a cox1 specific primer (Atcox1-1; table S1) using M-MLV (Moloney Murine Leukemia Virus) Reverse Transcriptase (RNase H minus, point mutant; Promega). The RNA template was degraded by adding 1/5 volume of 1 M NaOH to the reaction mixture and incubating at room temperature for 10 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Reverse transcription was performed using 2μg RNA of each sample and oligo (dT)18 primers by M-MLV reverse transcriptase (Promega, 28025013). Three independent biological samples were collected and analyzed ...
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bioRxiv - Plant Biology 2021Quote: ... One microgram of RNA was reverse transcribed using oligo(dT)18 and M-MLV reverse transcriptase II (Promega, https://worldwide.promega.com/). Quantitative real time PCR (qPCR ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein samples were re-solubilized in 6 M urea buffer followed by reduction and alkylation before digestion with trypsin (Promega) at 37°C for 18 hr ...
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bioRxiv - Biophysics 2020Quote: ... for 2 hours at 37° C and subsequently diluting to 1 M urea with 50 mM NH4HCO3 and finally adding 2% (w/w) trypsin (Promega) for 14 hours at 37° C ...
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bioRxiv - Cancer Biology 2020Quote: ... according to the manufacturer’s instructions and complementary DNA synthesis was carried out using M-MLV reverse transcriptase in the presence of RNasin RNase inhibitor (Promega) and an oligo(dT ...
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bioRxiv - Biochemistry 2022Quote: ... before being washed and resuspended in 2 M urea/PBS and trypsinized overnight with 0.5 μg /μL sequencing grade trypsin (Promega, V5111). Tryptic peptides were eluted off ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 6 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:25 (w/w ...
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bioRxiv - Immunology 2022Quote: The genomic DNA of the 5 assessed M strains were isolated and purified using the Wizard Genomic DNA Purification Kit (Promega). The growing of strains ...
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bioRxiv - Cell Biology 2022Quote: ... synthesis was done by using 1 μg RNA as template and the Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase (Promega), in 20 μl reaction volume according to the instructions manual ...
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bioRxiv - Plant Biology 2021Quote: ... Reverse transcription was performed using 2 μg of total RNA and oligo(dT)20 primers with M-MLV Reverse Transcriptase (Promega). All results shown include data from three independent experiments ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were then diluted to 4 M urea with 50 mM Tris-HCl pH 8 and Trypsin/Lys-C mix (Promega) was added to an enzyme:substrate ratio of 1:250 (w/w ...
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bioRxiv - Cancer Biology 2020Quote: ... according to the manufacturer’s instructions and complementary cDNA synthesis was carried out using M-MLV reverse transcriptase in the presence of RNasin RNase inhibitor (Promega) and an oligo(dT ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... retrovirus vector containing chick U6 promoter which expresses shRNA for gfp or Plzf was transfected into M/O chicken strain-derived embryonic fibroblast cells (CEF) cultured in 60-mm dish using Fugene HD transfection regent (Promega). CEF was cultured in DMEM-high glucose containing 10% FBS ...
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bioRxiv - Cell Biology 2021Quote: ... The sample was diluted 1:7 with 0.1 M NH4HCO3 before overnight digestion of proteins with trypsin (Promega, cat#V5113) at 30°C ...
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bioRxiv - Plant Biology 2021Quote: ... First-strand cDNA was synthesized from 2 μg total RNA using M-MLV reverse transcriptase with oligo-dT primer (Promega). Expression levels of germination-associated genes were measured by qRT-PCR analysis using LightCycler 480 SYBR Green I Master mix (Roche ...
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bioRxiv - Cell Biology 2020Quote: ... The reduced and alkylated protein lysates were diluted using 50 mM Tris-Cl to obtain a final concentration of 2 M urea in solution followed by overnight digestion with trypsin (1:1000)(Promega). Peptides were then desalted using c-18 Sep-Pack columns (Waters) ...
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bioRxiv - Genomics 2020Quote: ... Total RNA was purified using hot phenol–chloroform method as described previously (33) and reverse-transcribed to cDNA using M-MLV Reverse Transcriptase (Promega). A relative real-time quantitative PCR was then carried out for all the genes studied (Table S2 ...
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bioRxiv - Immunology 2020Quote: ... Recombinant DJ-1 protein (1 μM) was added to the transfected HEK293 cell lines and luciferase activities were measured using a Luciferase Assay System (Promega). 10 μg mL−1 of peptidoglycan (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2023Quote: ... samples were diluted with 50 mM NH4HCO3 to a final concentration of less than 2 M urea and were further digested overnight with 1:25 (w/w) trypsin (Promega) at room temperature ...
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bioRxiv - Immunology 2022Quote: ... followed by retrotranscription to cDNA with M-MLV RT, RNase H(–) point mutant (Promegain presence of buffer, nucleotides and RNasin Plus RNase Inhibitor (Promega). Reaction was then incubated at 40 °C for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: Proteins bound to beads were reduced with 2 mM DTT in 2 M urea buffer and sequencing grade trypsin (Promega) was added to a final concentration of 5 ng/μl ...
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bioRxiv - Microbiology 2023Quote: ... to a final urea concentration ˂ 2 M and then digested with modified trypsin (1:50 w/w) (Sequencing Grade, Promega) at 37°C for 3 h ...
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bioRxiv - Physiology 2023Quote: ... and reverse transcription was performed using an M-MLV reverse transcriptase with oligo-dT primers according to the manufacturer’s instructions (Promega, USA). For RT-PCR ...
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bioRxiv - Microbiology 2022Quote: ... Complementary DNA was synthetized from 500 ng total RNA by using random hexamers and moloney murine leukemia virus (M-MLV) reverse transcriptase (RT) Point Mutant Synthesis System (Promega). Quantification was performed by using the LightCycler 480 Universal Probe Library System (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... diluted by 4-fold to reduce urea to 2 M for the addition of trypsin (Promega, 1:50 w/w) to continue the digestion at 21°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... diluted by 4-fold to reduce urea to 2 M for the addition of trypsin (Promega, 1:50 w/w) to continue the digestion at 21ºC overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Afterwards the samples were diluted to decrease the concentration of urea up to 1 M and supplemented with additional amounts of sequencing-grade trypsin (Promega) (200 ng for pull-down samples and 1 ug for total lysate samples) ...
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bioRxiv - Cell Biology 2023Quote: ... A total of 500 ng of RNA were reverse-transcribed using M-MLV Reverse Transcriptase and Random Hexamer primers (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from one week-old Arabidopsis seedlings and reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and complementary DNA synthesis was carried out using M-MLV reverse transcriptase in the presence of RNasin RNase inhibitor (Promega). PCR primers are all intron spanning ...