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Citations for Promega :
451 - 500 of 1278 citations for Mouse Anti Human Papilloma virus type 16 716 325 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Two hundred nanograms of purified RNA were reverse-transcribed using Moloney Murine Leukemia Virus Reverse Transcriptase (MMLV RT, Promega). RT was primed with oligo dT(15 ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA was synthesized using 5μg of total RNA at 42 °C for 2 hours using avian myeloblastosis virus (AMV) reverse transcriptase (Promega). A 20 µL reaction containing 10 ng or 10 pg (for 16S rRNA ...
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bioRxiv - Cancer Biology 2020Quote: ... according to the manufacturer’s protocol and reverse transcribed with the Moloney Murine Leukemia Virus reverse transcriptase (M-MLVRT) (Promega) as follows ...
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bioRxiv - Microbiology 2022Quote: In vitro cytotoxicity of SMKIs on mock and/or virus infected A549 cells was determined using CellTiter-Glo 2.0 (CTG) Cell Viability Assay (Promega) according to manufacturer protocols.
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bioRxiv - Microbiology 2022Quote: Cytotoxicity of SMKIs on mock and/or virus-infected PCLS was determined using the LDH-Glo Cytotoxicity Assay (Promega) according to manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... for 1 h at 37 °C and cDNA was then synthesized using a Moloney murine leukemia virus (MMLV) reverse transcription kit with random primers following the manufactureŕs protocol (Promega). To check for the presence of remaining DNA ...
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bioRxiv - Microbiology 2021Quote: ... virus was harvested and infectivity assayed by serial titration on HeLa TZM-bl reporter cells using Bright-Glo (Promega).
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bioRxiv - Microbiology 2022Quote: ... First-strand cDNA generation from total RNA was generated using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega). Relative mRNA quantitation was done using the SYBR green quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Pathology 2021Quote: ... Cytopathic effects of the virus were measured after 3 days using the CellTiter-Glo luminescent cell viability assay (Promega), according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The viability of virus- and mock-infected cells were measured using Cell Titer Glow assay (CTG; Promega, Madison, USA). The luminescence was read with a PHERAstar FS plate reader (BMG Labtech ...
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bioRxiv - Neuroscience 2021Quote: ... The constructs (1.2 µg) were cotransfected with vesicular stomatitis virus G (600 ng) and pSPAX2 (800 ng) using FuGENE 6 (Promega). The lentiviral supernatants were collected 2 days after transfection ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and virus entry ability in the selected organs was evaluated by luciferase activity using the luciferase assay system (Promega) and a microplate luminometer ...
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bioRxiv - Microbiology 2021Quote: ... followed by a reverse transcriptase (RT)-step with Moloney Murine Leukemia Virus RT (MMLV-RT, Promega, Madison, WI, USA) in the presence of RNase inhibitor (RNAsin plus ...
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bioRxiv - Microbiology 2021Quote: ... the cells were lysed with a 1:1 ratio of virus to Bright-Glo luciferase assay media (Promega, #E2610) and the contents were analyzed on a luminometer (LUMISTAR Omega ...
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bioRxiv - Immunology 2021Quote: ... Reverse transcription was performed with Moloney murine leukemia virus reverse transcriptase (MMLV-RT) according to the manufacturer’s protocol (Promega) using 200 ng of total RNA ...
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bioRxiv - Microbiology 2022Quote: ... Virus was incubated with cells for 24 hours and cells were subsequently lysed with Renilla Luciferase Assay System (Promega) according to manufacturer instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Half of the virus containing media was aspirated and replaced with luciferase substrate (Bright-glo luciferase assay system, Promega) and incubated for 5 minutes at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: cDNA was synthesized using 1μg total RNA with 200 units moloney murine leukemia virus reverse transcriptase (Promega, Southampton UK), primed with 2μM oligo dT (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2023Quote: ... RNA was reverse-transcribed into cDNA using Maloney murine leukemia virus reverse transcriptase Taq polymerase (Promega, Madison, WI, USA). For quantitative RT-PCR ...
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bioRxiv - Microbiology 2023Quote: ... The virus infectivity was determined 48 h later by quantifying the luciferase activity (luciferase assay system, Promega, Madison, WI). Neutralization curves were normalized to virus only control ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was carried out with oligo(dT) primers using Moloney murine leukemia virus reverse transcriptase according to the manufacturer’s instructions (Promega). The qPCR reaction was performed on an Applied Biosystems QuantStudio3 thermocycler using a SYBR™ Green PCR Master Mix (Thermo Fisher scientific) ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay Virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system ...
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Bacteria Are a Major Determinant of Orsay Virus Transmission and Infection in Caenorhabditis elegansbioRxiv - Microbiology 2024Quote: ... Orsay virus RNA1 level was quantified by qPCR using 1 μl of 1/5 diluted cDNA (GoTaq Promega A6001) and run on QuantStudio 3 Real Time PCR system (RNA1 qPCR primers GW194 and GW195)23.
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bioRxiv - Cell Biology 2023Quote: ... wild-type and mutant tepsin-myc constructs were transfected using Fugene 4K (Promega) at 1.5:1 Fugene:DNA ratio following manufacturer protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated with anti-mouse or anti-rabbit HRP-conjugated secondary antibody for 30min (anti-mouse IgG HRP conjugate Promega #W402B, anti-rabbit IgG HRP conjugate Promega #W4011, 1:3000) and chemiluminescence was detected using Western Bright ECL spray (Advansta ...
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bioRxiv - Neuroscience 2021Quote: ... followed by the incubation with a secondary antibody anti-mouse IgG HRP conjugate (W4021, Promega, U.S.A.). All the primary antibodies were diluted 1/3000 in 10% Blocking One/TTBS (50 mM Tris ...
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bioRxiv - Molecular Biology 2021Quote: Primary antibodies and the dilutions used are as follows: anti-Halo (mouse, Promega G9211, 1:200); anti-Ezh2 (mouse ...
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bioRxiv - Neuroscience 2020Quote: ... all cells were incubated in the following: mouse anti-βIII tubulin (1:1000; 2 hr; Promega) and goat anti-mouse Alexa 546 (1:1000 ...
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bioRxiv - Cancer Biology 2019Quote: ... were secondary incubated with anti-mouse IgG (H+L) horseradish peroxidase coupled (1:3,000, W402b, Promega) and polyclonal anti-rabbit IgG (1:5000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Primary antibodies used were: rabbit and mouse anti-βGal (1/1000; Promega and MP Biomedicals, respectiveley), rat anti-Ser (1/1000 ...
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bioRxiv - Cancer Biology 2023Quote: ... Secondary antibodies were horseradish peroxidase-conjugated anti-mouse antibodies (cat. no. W402B; Promega, Madison, WI, USA) or rabbit IgG (cat ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were then incubated with an alkaline phosphatase-conjugated anti-mouse IgG (Promega, Madison, WI, USA) as the secondary antibody ...
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bioRxiv - Microbiology 2023Quote: ... and then incubated for another hour with an anti-Mouse IgG HRP-conjugate secondary antibody (Promega) (diluted 1:10 000 in PBST) ...
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bioRxiv - Genetics 2023Quote: ... and for 1 hour at room temperature in secondary antibody (1:4,000 anti-Mouse-HRP, Promega). In order to detect the endogenous Ptiwi09 protein ...
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bioRxiv - Microbiology 2023Quote: ... The membranes were blocked with PBS containing 5% skim milk and incubated with HRP-conjugated anti-human IgG1 antibodies (Promega, #W403B) overnight at 4 °C.
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bioRxiv - Microbiology 2021Quote: ... Total RNAs were isolated from the pellet using the Maxwell 16 LEV miRNA Tissue Kit (Promega) according to the manufacturer’s instructions and an extra TURBO DNase (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... and DNA extraction and purification were performed in a Maxwell® 16 DNA extraction system (Promega) using the LEV DNA kit (AS1290 ...
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bioRxiv - Neuroscience 2020Quote: ... Total tissue RNA was extracted using the Maxwell® 16 LEV simplyRNA Tissue Kit (Promega, AS1280). RNA quantification was performed with Qubit RNA Hs Assay kit (Thermo-Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... DNA from FFPE material was extracted Maxwell® 16 LEV RNA FFPE Purification Kit (Promega, AS1260).
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... total RNA was extracted using Maxwell® 16 Cell LEV Total RNA Purification Kits (Promega, US) and reversed transcribed into complementary DNA (cDNA ...
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bioRxiv - Microbiology 2021Quote: ... RNA isolations were performed using the Maxwell® 16 LEV Plant RNA Kit (Promega, Madison, USA). Real-time PCR was performed as described previously using the primers listed in Supplementary Table 3 (17).
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bioRxiv - Cancer Biology 2020Quote: ... STR profiling and analysis was performed using the PowerPlex® 16 HS System (Promega, Madison WI).
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bioRxiv - Cancer Biology 2022Quote: ... We validated all cell lines employing short tandem repeat analyses using PowerPlex® 16 system (Promega).
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bioRxiv - Plant Biology 2021Quote: ... Total RNA was extracted using the Maxwell 16 LEV Plant RNA Kit (Promega, Madison, WI, USA). RNA concentration was measured using the broad-range Quant-iT RNA Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted using the Promega Maxwell 16 with the LEV simplyRNA tissue kit (Promega AS1270), the Roche MagNAPure with the Cellular Total RNA Large Volume kit (Roche 05467535001) ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell line authentication was done by STR profiling with PowerPlex® 16 HS System from Promega. The IC-pPDXC-63 cell line was derived from the IC-pPDX-63 model ...
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bioRxiv - Evolutionary Biology 2021Quote: ... DNA was isolated from leaf tissue with Maxwell 16 tissue DNA purification kits (Promega, WI, USA), Qiagen DNeasy Plant Mini Kits (Valencia ...
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bioRxiv - Genomics 2021Quote: DNA was extracted using the Promega AS1030 Maxwell 16 Tissue DNA Purification Kit (Promega, Wisconsin, USA) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2022Quote: ... the 16s rRNA gene region was PCR amplified with GoTaq Green Master Mix (Promega, Madison, WI), 16S rRNA gene primers 27F (AGAGTTTGATCMTGGCTCAG ...
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bioRxiv - Microbiology 2023Quote: RNA was extracted from bacterial culture samples using Maxwell® 16 LEV miRNA Tissue Kit (Promega). cDNA was produced using random hexamers as primers and the GoScriptTM Reverse Transcription kit from Promega ...