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Citations for Promega :
451 - 500 of 4498 citations for Human Procollagen II C Terminal Propeptide PIICP CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Proteins were digested overnight at 37°C with 1µg trypsin (Promega). Peptides were desalted on C18 StageTips ...
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bioRxiv - Cell Biology 2021Quote: ... They were then digested with mass spectrometry grade Lys-C (Promega) at a protein:Lys-C ratio of 100:1 (w/w ...
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bioRxiv - Molecular Biology 2023Quote: ... and overnight ligation at 4°C (T4 DNA ligase, Promega, USA). Ligated chimeras were transformed into DH5α (ThermoScientific ...
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bioRxiv - Microbiology 2023Quote: ... stored at −80 °C and analysed using Dual-luciferase substrate (Promega) in a FLUOstar Optima luminometer (BMG labTech) ...
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bioRxiv - Pathology 2023Quote: ... MS-grade trypsin and Lys-C were from Promega (Madison, WI). All other chemicals and reagents were pu0072chased from Sigma-Aldrich (St ...
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bioRxiv - Biochemistry 2023Quote: ... 5mM DTT containing 10 ng/μL trypsin/Lys-C mixture (Promega), and incubated overnight at 25 °C ...
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bioRxiv - Developmental Biology 2023Quote: ... and digested with trypsin/Lys-C mix (V5071, Promega, Madison, WI). The resulting peptides were cleaned up with Acclaim™ PepMap™ 100 C18 spin columns (SEM SS18V ...
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bioRxiv - Plant Biology 2023Quote: ... Lys-C digest was further digested with 0.1 μg trypsin (Promega) with shaking at 1,000 rpm at 37°C overnight ...
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bioRxiv - Cell Biology 2023Quote: ... Beads were then proteolyzed with Trypsin/Lys-C Mix (Promega, V5071) at a 25:1 protein ...
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bioRxiv - Cell Biology 2022Quote: ... cDNA was synthesized in duplicates from 100 ng total RNA using ImProm II reverse transcriptase (Promega), RNAsin (Promega ...
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bioRxiv - Microbiology 2021Quote: ... with a Luciferase Assay Reagent II and a Stop & Glo® Reagent (Promega Madison, WI, USA) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA synthesis was carried out using the Improm-II reverse transcriptase according to Manufacturer’s instructions (Promega). Gene expression analysis was carried out using the Brilliant SYBR Green QPCR Reagents on a Stratagene MX3000P qPCR system according to the instructions of the manufacturer and the primers described in Table 3 ...
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bioRxiv - Cell Biology 2021Quote: ... Complementary DNA (cDNA) was synthesized following manufacturer’s instructions using the ImProm II Reverse Transcriptase system (Promega) from 500 ng of total RNAs for mycelial samples and 650 ng of total RNAs for sheath samples ...
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bioRxiv - Plant Biology 2020Quote: ... First-strand cDNA was synthesized from purified RNA with ImProm-II™ Reverse Transcription System (Promega) and diluted 10 times for RT-qPCR to detect related genes with specific primers ...
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bioRxiv - Immunology 2021Quote: ... We then reversed transcribed RNA into cDNA by using Improm II reverse transcriptase (Promega, Madison, WI) and random hexamer primers (Genelink ...
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bioRxiv - Microbiology 2022Quote: ... Recombinant EbfC was expressed in Escherichia coli Rosetta II cells and purified using MagneHis Particles (Promega) as previously described (24 ...
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bioRxiv - Neuroscience 2023Quote: Complementary DNA (cDNA) was synthesized from RNA using the ImProm-II Reverse Transcription System (Promega, #A3800) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... We then reversed transcribed RNA into cDNA by using Improm II reverse transcriptase (Promega, Madison, WI) and random hexamer primers (Genelink ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was reverse-transcribed into cDNA using ImProm-II™ Reverse Transcription System (Promega, WI, USA). Real-time PCR was conducted using the SYBR green PCR reagent (Roche ...
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bioRxiv - Molecular Biology 2023Quote: ... Recovered RNA from all trajectories was reverse transcribed using ImProm-II Reverse Transcriptase (Promega, Madison, WI) and PCR amplified using Taq DNA polymerase for 10 cycles to generate the transcription template for the next round of selection.
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bioRxiv - Neuroscience 2024Quote: Complementary DNA (cDNA) was synthesized from RNA using the ImProm-II Reverse Transcription System (Promega, #A3800) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2019Quote: ... and human 293T cells as producer cells using the FUGENE HD transfection reagent (Promega), as described [50] ...
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bioRxiv - Pathology 2019Quote: ... human primary aortic SMCs were transfected with pGL4.34 Vector plasmids (E1350; Promega, Madison, WI) using Effectene Transfection Reagent (301425 ...
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The absence of C-5 DNA methylation in Leishmania donovani allows DNA enrichment from complex samplesbioRxiv - Molecular Biology 2020Quote: ... we used a 1/1500 artificial mix of promastigote DNA and human DNA (Promega) to reflect the median ratio found in clinical samples ...
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bioRxiv - Neuroscience 2022Quote: Open reading frames of human OR genes were subcloned into pCI (Promega, WI, USA) with a Rho-tag (the sequence encoding the first 20 amino acids of rhodopsin ...
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bioRxiv - Immunology 2023Quote: ... tailed with complete TRAC and TRBC1 gene segments amplified from human genomic DNA (Promega), respectively ...
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bioRxiv - Biochemistry 2024Quote: ... 24 hours after transient transfection with mouse or human ENPP3 via Fugene 6 (Promega), the media was gently removed and replaced with serum-free DMEM supplemented with 1% insulin-transferrin-selenium-sodium pyruvate (ThermoFisher ...
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bioRxiv - Bioengineering 2021Quote: ... A standard curve was prepared through cloning method using pGEM(R)-T Easy Vector System II (Promega) and JM109 Competent Cells (Promega) ...
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bioRxiv - Developmental Biology 2019Quote: ... cDNA was generated from 500 μg purified RNA with the ImProm-II™ Reverse Transcription System (Promega) primed by random primers ...
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bioRxiv - Molecular Biology 2020Quote: ... A 5µl aliquot of the sample was added to a 10µl ImProm-II reverse transcription reaction (Promega). The ssDNA product was used as a template for 25 cycles of PCR using a 50µl GoTaq reaction (Promega).
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bioRxiv - Microbiology 2020Quote: ... and lysates were read on a Promega GloMax Multi Detection unit using Luciferase Assay Reagent II (Promega) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2021Quote: ... ImProm-II™ Reverse Transcription System and GoTaq® DNA polymerase were purchased from Promega (Madison, WI). The RNA Clean & Concentrator™-5 was purchased from Zymo Research (Irvine ...
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bioRxiv - Immunology 2021Quote: ... TCR- and CD3-encoding pMIG II vectors were used to transiently transduce HEK293T cells using FUGENE (Promega). Cells were harvested after 48h and assessed for CD3/TCR expression and CD1d-tetramer binding using flow cytometry ...
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bioRxiv - Microbiology 2023Quote: ... Reverse transcription was conducted on the normalized RNA samples using the ImProm-II Reverse Transcription System (Promega). After addition of 1 μl of random primers to 9.5 μl of sample ...
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bioRxiv - Molecular Biology 2023Quote: ... HDAC activity of three technical replicates was measured using the HDAC-Glo I/II Assay (Promega, #G6420) following the manufacturer’s protocol on-bead with an Envision plate reader (Perkin Elmer) ...
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bioRxiv - Cell Biology 2023Quote: ... PCR products were subsequently cloned into vectors using pGEM-T Easy Vector System II (Promega, Cat# A1380) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... the 60°C annealing/extension temperature recommended by the mastermix manufacturer (Promega) produced more robust amplification (data not shown).
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bioRxiv - Microbiology 2019Quote: ... Proteins were digested overnight at 37°C with 800 ng trypsin (Promega). Digestion was stopped by adding 5 μl TFA (Trifluoroacetic acid ...
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bioRxiv - Biochemistry 2021Quote: ... and finally digested overnight at 37 °C with 375 ng Trypsin (Promega). The peptides were extracted from the gel twice using 100 μl of 50 % acetonitrile and 0.25 % TFA buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... and 30 s at 68°C or through GO taq polymerase (Promega) for 2 min at 95°C followed by 25 cycles of 30 s at 95°C ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were digested with 1 μg trypsin/Lys-C mix (Promega) at 37 °C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... Proteolytic digestion was carried out with a trypsin/Lys-C mixture (Promega) incubated for 18 h at 37°C at a protein to protease ratio of 25:1 ...
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bioRxiv - Cell Biology 2021Quote: ... 60°C for 1 minute) using Go-Taq qPCR Master Mix (Promega) on a QuantStudio™ 7 Flex Real Time PCR System (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2021Quote: ... the plasmid was re-ligated overnight at 16°C (ligase from Promega) and transform in Stbl4 bacteria ...
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bioRxiv - Microbiology 2020Quote: ... The samples were treated with trypsin/Lys-C protease mix (Promega, V5072) at a protease:protein ratio of 1:25 for 16 h at 37°C ...
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bioRxiv - Biochemistry 2021Quote: ... For digestion Typsin/Lys-C mass spectrometry grade proteases mixture from Promega Corporation was used ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were digested at 37°C for 18 hr with trypsin (Promega) at a concentration of 1:25 (w/w ...
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bioRxiv - Biophysics 2020Quote: ... Samples were digested overnight at 37 °C with 1 µg trypsin (Promega).
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bioRxiv - Biochemistry 2022Quote: ... Proteins were digested over night at 37 °C with trypsin (V5111, Promega) at a 1:20 enzyme to protein mass ratio ...
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bioRxiv - Developmental Biology 2022Quote: ... which were digested with 1:50 Arg-C protease (Promega, cat. #V1881): histone protein at 37°C for 12-16 hours ...