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Citations for Promega :
451 - 500 of 537 citations for H Leu arg pro gly nh2 2hcl since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant C-terminally HA tagged Su(H) was produced using in vitro coupled transcription/translation (TNT with rabbit reticulocyte extracts; Promega Inc.) from DNA matrices generated by PCR using Su(H)-HA forward and reverse primers combined with the Su(H ...
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bioRxiv - Cell Biology 2021Quote: ... 30 min) and diluted to 1M urea with 200mM ammonium bicarbonate for trypsin digestion (1μg, 37°C, 8 h, Promega cat # V5113).
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bioRxiv - Microbiology 2021Quote: ... Remaining ATP was readout after 3 h incubation using Kinase-Glo® Luminescent Kinase kit following manufacturer’s instructions (Promega, WI, USA).
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bioRxiv - Microbiology 2022Quote: ... After 1 h cells were washed in PBS and cultured for additional 24 h before the evaluation of the Luc expression using the Steady-Glo® Luciferase reagent (Promega), which were added to the cell for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... Pseudoviruses stock were titrated by infecting 293T-ACE2 cells and luciferase activity was determined following a 44-48 h incubation period at 37°C and 5% CO2 by addition Bright-Glo Luciferase Assay System (Promega, E2650) using a microplate reader (TECAN ...
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bioRxiv - Cancer Biology 2022Quote: ... treated as indicated for 72 h and assessed for cell viability using the Cell Titer-Glo® Luminescent Cell Viability Assay (Promega) or Cell Counting Kit-8 (CCK-8 ...
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bioRxiv - Microbiology 2020Quote: ... Cells were further cultured for 72 h at 37°C with 5% CO2 and lysed in luciferase substrate buffer (Bright-Glo; Promega, Poland). Lysates were transferred onto white 96-wells plates and luciferase levels were measured on a microplate reader Gemini EM (Molecular Devices ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Infected SupT1 cells were cultured for another 48 h and directly lysed with 100 uL of Bright-Glo Luciferase Reagent (Promega, #E2610). Cell lysate was measured for luciferase activity using a LUMIstar Omega microplate luminometer (BMG Labtech) ...
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The human sperm basal body is a complex centrosome important for embryo pre-implantation developmentbioRxiv - Developmental Biology 2021Quote: ... The resulting protein extract was first diluted to 2M urea for overnight digestion with LysC (Wako, USA) at 37°C and then diluted 2-fold for 8 h digestion with trypsin (Promega, USA) at 37°C.
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bioRxiv - Biochemistry 2021Quote: ... Cell viability following 72 h treatment of cells with the respective compounds was determined via CellTiter-Glo® Luminescent Cell Viability Assay (Promega), following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... inhibition following 42 h treatment of cells with the respective compounds was determined via CellTiter-Glo® Luminescent Cell Viability Assay (Promega). Luminescent signal was recorded using a CLARIOstar multi-mode microplate reader (BMG Labtech ...
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bioRxiv - Microbiology 2021Quote: ... target cells were plated at 2.5 × 104 cells per well in 96-well plates or at 2 × 105 cells per well in 12-well plates and infected for 24-48 h before lysis and Renilla (and Firefly) luciferase activity measure (Dual-Luciferase® Reporter Assay System Promega) or fixation with 2% paraformaldehyde (PFA)-PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... and the appropriate protein amount (60 μg for baseline expression experiments and 1mg for perturbation experiments) was digested with LysC (1:100, Wako, #121-05063) for 4 h and Trypsin (1:75, Promega, #V5113) overnight ...
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bioRxiv - Plant Biology 2022Quote: ... Firefly luciferase and REN activity were measured after 48 h in extracts from infiltrated leaf discs using the Dual-Luciferase Reporter Assay System (Promega, USA) according to the manufacturer’s protocol ...
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bioRxiv - Physiology 2024Quote: ... The toxicity of MNP was determined 24 h upon MNP treatment using the LDH-toxicity Assay (Promega Corp, Fitchburgh, WI, US) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Reverse transcription was achieved from 1 𝜇g of total RNA with M-MLV reverse transcriptase (RNase H minus, Point Mutant, Promega, USA) using an anchored oligo(dT)20 primer ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant was diluted 5-fold with 50 mM Tris-HCl pH 8.0 and digested for 12 h at 37°C using sequencing grade trypsin (Promega cat#: V511A) at 1/100 the mass of total protein ...
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bioRxiv - Cell Biology 2023Quote: ... samples were resuspended in 50 µl of 50 mM ABC buffer and digested at 37°C for 16 h using 1 µg of trypsin (Trypsin Gold, Promega, V5280) and 1 µg of LysC (Wako Pure Chemical Corporation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 h later 5 µl/well of NanoBRET™ Nano-GloR Substrate (10 µl/ml in DMEM no phenol red, Promega) was added and 460 nm donor and 618 nm acceptor signals were read within 10 min of substrate addition using CLARIOstar microplate reader (Mandel) ...
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bioRxiv - Cell Biology 2023Quote: ... the 200k pellets were digested for 2 h at 37°C with 0.4 µg of Trypsin/Lys-C (Promega CAT#: V5071) and then overnight by adding 0.4 µg of Trypsin/Lys-C ...
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bioRxiv - Cell Biology 2023Quote: ... or pCEP4-OCT4-T234A-S235A (addgene, 40630) or pCEP4-OCT4-T234E-S235E (addgene, 40631) for 48 h using FuGENE 6 (Promega, E2691) according to the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: ... at 3,000 cells/well in 50 μL media containing IFN-α for 72 h prior to cell lysis with 50 μL CellTiterGlo 2.0 (Promega, G9243). The reaction was incubated in the dark for 20 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... After 24 or 48 h, luciferase expression (relative light units, RLU) was measured with the Bright-Glo™ Luciferase Assay System (Promega).
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bioRxiv - Developmental Biology 2024Quote: ... The luciferase assay was carried out 48 h after transfection with the Nano-Glo Dual-Luciferase Reporter Assay System (Promega N1610) per manufacturer instructions ...
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bioRxiv - Physiology 2024Quote: ... further steps were performed according to the manufacturer’s protocol using proteases trypsin and Lys-C (2 h at 47° C, 1:15 and 1:30, respectively, Promega, USA). Peptides (20 μg ...
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bioRxiv - Microbiology 2024Quote: ... Contaminating DNA in the samples were removed through incubation at 37°C for 2 h using RNase-free DNase I (Promega, USA). All RNAs in the samples were converted into cDNA using a cDNA EcoDry Premix (Clontech ...
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bioRxiv - Physiology 2024Quote: ... and hydrolyzed into the S-Trap column using trypsin and Lys-C (2 h at 47 °C, 1:15 and 1:30, respectively, all Promega, USA) in 40 μl 50 mM triethylammonium bicarbonate according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2024Quote: ... Medium was replaced 12 h after transfection and 48 h after transfection cells were lysed with 50 μL 1x passive lysis buffer diluted in water (Promega, E194A). Cells were frozen at -70°C for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... These near UV-active compounds were instead screened with luminescent assays using the NAD(P)H-Glo™ Detection System (Promega), which monitors the formation of NAD(P)H with the aid of two coupling enzymes ...
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bioRxiv - Cell Biology 2020Quote: ... 1 μg of pCS2-HA-NFATc3 was incubated for 2 h at 30°C in 50 μl of the TNT® SP6 coupled wheat germ extract system (Promega, #L5030), according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... secondary antibody staining was performed for 1 h at room temperature using anti-rabbit HRP or anti-mouse HRP secondary antibodies (Promega, 1:10,000) in blocking buffer ...
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bioRxiv - Biochemistry 2021Quote: Pre-treatments were administered as described and glutamate added for 24 h prior to assessing viability using Cell-Titer Glo reagent (Promega; cat. PRG7572) or MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide ...
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bioRxiv - Cell Biology 2021Quote: ... and 1-3μl aliquots were taken for reverse transcription (RT) using the Moloney murine leukemia virus RT RNase H (−) (Promega, Madison, WI, USA), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The antibody-virus inoculum was replaced with fresh culture medium eight hours post-infection and cells processed 48 h post-infection with Nano-Glo luciferase assay system (Promega, Madison, WI). Luminescence was measured using an Enspire plate reader (PerkinElmer ...
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bioRxiv - Biophysics 2020Quote: ... P1KO cells were transiently transfected in a 6-well plate (the cells were seeded 8 h before transfection) in the presence of ruthenium red (10 μM) and mouse Piezo1 (3 μg) using Fugene6 (Promega, Madison, WI). 20–30% of cells showed positive GFP expression indicating successful Piezo1 transfection.
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bioRxiv - Immunology 2022Quote: ... Pseudovirus titers were measured by infecting 293T-ACE2.TMPRSS2s cells for 48 h prior to measuring luciferase activity (luciferase assay reagent, Promega, Madison, WI). For neutralization assays ...
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bioRxiv - Cell Biology 2022Quote: ... The viability of hUC-MSCs after 24 h exposure to increasing concentrations of GNRs was determined by the CellTiter-Glo™ Luminescent Cell Viability Assay (Promega Corporation), which generates luminescent signals based on ATP levels ...
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bioRxiv - Pathology 2021Quote: ... 30 μg of protein was digested with Lys-C (FUJIFILM Wako Chemicals Europe GmbH, Germany) for 4 h and subsequently with modified porcine trypsin (Promega, WI, USA) for 16 h at 37 °C.
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bioRxiv - Microbiology 2020Quote: ... Pseudovirus titers were measured by infecting different cells for 48 h prior to measuring luciferase activity (luciferase assay reagent, Promega, Madison, WI), as described previously (51) ...
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bioRxiv - Immunology 2021Quote: ... H1299 cells were incubated with the pseudovirus for 24 h followed by luciferase assays performed using NanoGlo Luciferase Assay System (Promega, Madison, WI). In one set of experiments ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were treated with IFNα (1000 U/mL) for 12 h and were harvested to conduct dual-luciferase reporter assay (Promega, Madison, WI). Firefly luciferase activity values were normalized to Renilla luciferase activity ...
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Development of immortalized rhesus macaque kidney cells supporting infection with a panel of virusesbioRxiv - Microbiology 2022Quote: ... After 72 h medium was removed and cells were lysed in 50 μl Luciferase Cell Culture Lysis Reagent (Promega, Madison, WI, USA). Luciferase activity was meastued in a Plate Chameleon V (Hidex ...
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bioRxiv - Plant Biology 2022Quote: Total RNA of TH3o and O23 was extracted at different stages (24 and 48 h) of barley infection using the SV Total RNA Isolation System (Promega, WI, USA). One microgram of total RNA was used to prepare each sequencing library with the NEBNext Ultra II Directional RNA library prep kit (New England Biolabs Japan ...
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bioRxiv - Microbiology 2024Quote: 100,000 HEK-293T cells were co-transfected with 150 ng GFP/GFP-IpaH4/GFP-IpaH4C339S and 350 ng target vectors for 48 h using FuGENE (Promega; Table S4). Cells were harvested in RIPA buffer containing 100 µM PMSF and protease inhibitor cocktail (Abcam ...
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bioRxiv - Microbiology 2022Quote: ... Luminescence readings were taken at 0 and 72 h post-invasion using the Renilla-Glo® Luciferase Assay System (Promega cat. E2750) and the GloMax® Discover Multimode Microplate Reader (Promega) ...
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bioRxiv - Molecular Biology 2023Quote: ... Japan) at 37 C for 2 h before digestion overnight with 1 ug Trypsin/Lys-C mix (V5071, Promega, Madison, WI, USA) at 37 C ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were rinsed 5 times for 8 min in PBS-tween 0.2% and incubated with secondary antibodies for 2 h at room temperature under agitation (HRP anti-mouse, 1:10000, Promega cat. # W4021). Membranes were further rinsed 5 times for 8 min in PBS-tween 0.2% ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The activity of the two luciferase enzymes was measured 24 h after co-transfection following the manufacturer’s instruction of Dual-Luciferase® Reporter Assay System (Cat# E1910, Promega, Wisconsin, USA). Nine biological replications were carried out for each transfection.
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody staining was performed for 1 h at room temperature using antirabbit HRP or anti-mouse HRP secondary antibodies (Promega,1:10,000) in blocking buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were lysed 24 h after transfection and luciferase activity was measured with the Dual-Luciferase Reporter Assay kit (Promega Cat. # E1960) according to the manufacturer’s protocol using a FlUOstar plate reader (BMG Labtech) ...