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Citations for Promega :
451 - 500 of 704 citations for Creatine Kinase M Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Reverse transcription of 2 μg RNA was performed using M-MLV reverse transcriptase (Promega, WI, USA). The PCR-based amplification was done using synthesized-cDNA ...
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bioRxiv - Molecular Biology 2023Quote: 500 ng of total RNA were converted to cDNA using M-MLV Reverse Transcriptase (Promega, M1705) and Oligo(dt ...
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bioRxiv - Microbiology 2022Quote: ... The solution was diluted to below 1 M urea and digested using sequencing grade Trypsin (Promega). The proteolytic peptides were finally desalted using an Oasis HLB solid phase extraction well plate (Waters ...
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bioRxiv - Systems Biology 2022Quote: ... The proteins were diluted to 0.1 M ammonium bicarbonate and digested overnight with Trypsin (Promega, V5113) at 37°C with a protein-to-enzyme ratio of 50:1 ...
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bioRxiv - Immunology 2023Quote: ... The cDNA was synthesized from 2μl of the eluted RNA using M-MLV reverse transcriptase (Promega) and random hexamer primers ...
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bioRxiv - Molecular Biology 2023Quote: ... First-strand cDNA synthesis was performed from 1 µg RNA using M-MLV Reverse Transcriptase (Promega) and random hexamer primers (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2023Quote: ... RNA (1.2 µg) was utilized to reverse transcript with M-MLV Reverse Transcriptase kit (M1701, Promega), Random Hexamer Primers (48190011 ...
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Deciphering the polycistronic nature of Mycobacterium tuberculosis lipoproteins of unknown functionsbioRxiv - Microbiology 2023Quote: ... The cDNA synthesis was performed using 1 μg of RNA with M-MLV reverse transciptase (Promega) (RT+ ...
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bioRxiv - Cancer Biology 2023Quote: ... The total RNA was next reverse-transcribed to cDNA by M-MLV reverse transcriptase (Promega, M1701) 0.5 mg/mL Oligo(dT)15 primers were added to 200 ng of RNA and was incubated at 70°C for 5 mins ...
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bioRxiv - Plant Biology 2023Quote: ... Four microgram of extracted RNA sample was reverse transcribed using the M-MLV Reverse Transcriptase (Promega) from each RNA sample ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1-1 µg of RNA was reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega) and oligo(dT ...
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bioRxiv - Physiology 2024Quote: ... Reverse transcription was performed using oligo(dT) primers and M-MLV Reverse Transcriptase (Promega, Madison, WI) at 42°C for 1 hour according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Digests were diluted further to a 1.5 M urea concentration and 5 µg of trypsin (Promega) was added for 6 hours at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... trypsin digestion was performed after diluting urea to <1.2 M and using 0.5 µg trypsin (Promega), and processed peptides were dissolved in 20 µl of buffer A ...
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bioRxiv - Cancer Biology 2019Quote: ... ATP solution (5 μL of a 100 μM solution containing 0.1 μg 4:1 glycine:tyrosine peptide substrate (Promega)) was added and incubated at 37 °C for 20 min before the addition of ADP-Glo reagent (5μL ...
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bioRxiv - Biochemistry 2021Quote: ... Aliquots of reagent were used for sample labeling of a serial dilution of K562 peptide digest standard (Promega) using all heavy N labeled channels that day following manufacturer instructions ...
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bioRxiv - Biophysics 2021Quote: ... Human Gβ with a C-terminal 15-amino-acid polypeptide linker followed by a HiBiT (peptide 86, Promega) and human Gγ were cloned into pFastBac vector ...
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bioRxiv - Biochemistry 2022Quote: ... Rat Gβ1 with an N-terminal MHHHHHHSSGLVPRGSHMASHHHHHHHHHH-tag (His16) was fused with the SmBiT subunit (peptide 86, Promega)20 via a 15 amino acid (GSSGGGGSGGGGSSG ...
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bioRxiv - Plant Biology 2023Quote: ... Total proteome peptide pools were generated by sample digestion overnight with 1:100 sequencing grade trypsin (V5113; Promega). Generated peptide pools were quantified by Nanodrop ...
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bioRxiv - Cell Biology 2023Quote: ... Peptide digestion was performed by adding 1µg of trypsin (Trypsin Gold, Mass Spectrometry Grade, Promega Corporation, WI, USA) and incubating each sample overnight at 30°C ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were diluted to 1 M urea with 50 mM ambic and digested with Trypsin (Promega #V5111) for 10 hours at 1:125 enzyme:protein at 37°C ...
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bioRxiv - Molecular Biology 2019Quote: ... Reverse transcription was performed with random hexamer primers (R6) and M-MLV reverse transcriptase (Promega, Madison, WI) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... cDNA was generated with the DNase-treated RNA (5μg) and M-MLV reverse transcriptase (Promega, Madison, WI). The cDNA was then used as a template for qRT-PCR to determine relative gene expressions of TuCPR in different life stages of the susceptible T ...
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bioRxiv - Biochemistry 2020Quote: ... The reverse transcriptase M-MLV RT as well as the T4 DNA ligase were obtained from Promega. The pGEM-T Vector kit was purchased from Promega ...
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bioRxiv - Plant Biology 2020Quote: ... We synthesized cDNA using 2.0 μg of total RNA per sample and M-MLV Reverse Transcriptase (Promega) and poly-T primers (Sigma-Aldrich).
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bioRxiv - Plant Biology 2021Quote: ... 100 ng of the recovered RNA was used as a template for M-MLV reverse transcriptase (Promega). The PCR reactions were performed in a T-ADVANCED S96G (Biometra ...
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bioRxiv - Immunology 2021Quote: ... and reversed transcribed with random hexamers (IDT) using Moloney murine leukemia virus (M-MLV) reverse transcriptase (Promega) according to the manufacturers’ instructions ...
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bioRxiv - Immunology 2020Quote: RNA was extracted from ear homogenates and reverse transcribed into cDNA using M-MLV reverse transcriptase (Promega). Real-time PCR was performed on an ABI PRISM 7500 sequence detection system (Applied Biosystems ...
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bioRxiv - Microbiology 2019Quote: ... 1 μg of total RNA template was reverse transcribed to cDNA by M-MLV reverse transcriptase (Promega) using specific stem-loop RT primer and under the following conditions ...
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bioRxiv - Physiology 2021Quote: ... Complementary DNA (cDNA) was synthesized from total RNA (300 ng) using M-MLV Reverse Transcriptase (Promega, #M1701) as per supplier’s protocol (1hr at 37°C followed by 10 min at 70°C) ...
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bioRxiv - Physiology 2021Quote: ... Subsequent first strand synthesis of cDNA was conducted following the protocol of M-MLV reverse transcriptase (Promega). qPCR was conducted with SYBR-Green reagent and analyzed with the Quantstudio Real-Time PCR System ...
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bioRxiv - Biochemistry 2022Quote: ... urea was diluted to 1 M by adding 50 mM ammonium bicarbonate and 2 μg Trypsin (Promega) per 100 μg protein ...
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bioRxiv - Genomics 2022Quote: ... Reverse transcription was carried out using moloney-murine leukemia virus (M-MLV) reverse transcriptase kit (Promega, USA). Reverse transcription was completed in a 30 µL final volume reaction containing 400 U M-MLV and 1 mM dNTP mix (Invitrogen ...
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bioRxiv - Genetics 2022Quote: Total RNAs were reversed into first-strand cDNA using the M-MLVRT reverse transcription kit (Promega, USA). qRT-PCR was performed with SYBR Premix Ex Taq (TaKaRa Biotech Co. ...
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bioRxiv - Neuroscience 2023Quote: ... The revers transcription (RT) reaction was performed by M-MLV reverse transcription kit (Promega, Madison, WI, USA).
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bioRxiv - Bioengineering 2023Quote: ... 250 ng to 1 μg of RNA were reverse transcribed using M-MLV Reverse transcrtiptase (Promega, #M1705) and oligo(dT ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was retrotranscribed into cDNA using M-MLV reverse transcriptase with Oligo(dT)15 primers (Promega) according to manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: ... 1 µg of total RNA was used to synthesize cDNA using M-MLV reverse transcriptase kit (Promega) using the supplier’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Protein marker was purchased from Fermentas (Beijing) and M-MLV reverse transcriptase was purchased from Promega (Beijing). Homologous recombinant kit was purchased from Clone Smarter (Beijing) ...
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bioRxiv - Genomics 2023Quote: ... 1 µg of Dnase-treated total RNA was reverse transcribed using the M-MLV Reverse Transcriptase (Promega) and Random Hexadeoxynucleotides (Promega ...
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bioRxiv - Immunology 2023Quote: ... The total RNA extracted was reverse transcribed using M-MLV reverse transcriptase (Promega, Madi-son, WI, USA) and using random primers for initiation ...
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bioRxiv - Neuroscience 2024Quote: ... along with a Ten-m expression construct and Actin-GAL4 using the FuGENE HD transfection reagent (Promega). After 72 hours ...
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bioRxiv - Cell Biology 2022Quote: ... For phospho-peptide samples the volume was increased to 2,000 μl (7-fold dilution) and 30 μg Trypsin Gold (Promega) was added to each sample ...
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bioRxiv - Neuroscience 2022Quote: ... The mixture was then incubated with 1 μg of AKT substrate II peptide (CKRPRAASFAE) as the Pak4 substrate (Promega) or 1 μg of GST-Raf13 as the Pak1 substrate with a total volume of 5 μL in a 384-well plate (PerkinElmer ...
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bioRxiv - Immunology 2019Quote: ... then washed three times in lysis buffer and two times in ammonium biocarbonate (50mM) before peptide digestion (V5280, Promega). Peptides were loaded onto a 3-cm self-packed C18 capillary pre-column (Reprosil 5 μM ...
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bioRxiv - Cancer Biology 2021Quote: ... EV protein samples were alkylated the next day with 50 mM iodoacetamide for 2 hours at ambient temperature in the dark and then digested into peptides with 1.2 µg proteomics-grade trypsin/LysC (Promega) according to the SP3 protocol described by Hughes et al (2019) ...
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bioRxiv - Physiology 2022Quote: ... The proteins were cleaved into tryptic peptides by addition of 25 μl of a 0.2 μg/μl trypsin (Promega) in 50 mM ammonium bicarbonate and incubation (18 h ...
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bioRxiv - Molecular Biology 2022Quote: ... The human Gβ1 with a C-terminal 15-amino acid polypeptide linker was followed by a HiBiT (peptide 86, Promega), and the scFv16 was modified with an N-terminal GP67 signaling peptide and a C-terminal 8× histidine tag ...
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bioRxiv - Molecular Biology 2023Quote: ... Quality control of the instrument setup was monitored using the Promega 6x5 LC-MS/MS Peptide Reference Mix (Promega) and analyzed with the PReMiS software (version 1.0.5.1 ...
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bioRxiv - Bioengineering 2023Quote: ... The reaction was initiated by the addition of 1-250 nM acylated peptide (Vivitide) and a 1:10,000 dilution of LgBiT subunit of the NanoBiT luciferase (Promega) and read using an Envision plate reader (XCite 2105 ...