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Citations for Promega :
451 - 500 of 1324 citations for Anti Rat IgG H+L Antibody Conjugated to HRP since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... using MMLV reverse transcriptase RNase H Minus (Promega). Purified resuspended cDNAs were sequenced on a CEQ 8000 capillary electrophoresis sequencer (9+) ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated with 10µM Coelenterazine-h (Promega) in the dark for 2 hours before recording luminescence in a plate reader (Optima FLUOStar ...
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bioRxiv - Genetics 2022Quote: Endoglycosidase H (EndoH) (Promega V4871, 500 U/μL) was used to deglycosylate Ina1-GFP-containing samples ...
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bioRxiv - Biochemistry 2022Quote: ... the proteins were deglycosylated by Endo-H (Promega) followed by PNGaseF (Promega ...
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bioRxiv - Molecular Biology 2024Quote: ... Media was then replaced with coelenterazine-H (Promega) added at a 1:100 dilution and vehicle and agonists were prepared in HBSS at 10x concentration ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies: mouse anti-β-tubulin (E7, Developmental Studies Hybridoma Bank) and rabbit anti-Halo (G9281, Promega). Secondary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by the addition of 5-μL Endo-H reaction buffer and 5-μL Endo-H (Promega Cat#PRV4875, 2,500-U). Deglycosylation proceeded for 4-hours at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were treated 8 h post-transfection with or without IFN-α (1000 U/ml) before lysis 16 h later using Passive Lysis Buffer (Promega). Firefly and Renilla luciferase activity was then determined in a dual luciferase assay ...
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bioRxiv - Cancer Biology 2021Quote: ... The viability of the cultures was determined 24 h and 48 h after EV treatments by using the CellTiter-Fluor cell viability assay kit from Promega (G6080) following manufacturers instructions ...
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bioRxiv - Microbiology 2023Quote: ... The presence of glycosylated proteins in the cell lysates was assessed using Promega Endoglycosidase H (Endo H) assay reagents (Promega V4871). Briefly ...
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bioRxiv - Microbiology 2022Quote: ... The samples were incubated at 30 °C and luminescence was quantified after 2 h and 4 h post infection (20 μl sample plus 20 μl substrate, Nano-Glo® Luciferase Assay System, Promega). The sample was carefully removed from the upper part of the tube without disturbing the sedimented erythrocytes in the blood samples.
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bioRxiv - Physiology 2024Quote: Total RNA from naked mole-rat and human fibroblasts and from naked mole-rat tissues was extracted using the ReliaPrep™ RNA Miniprep System (Promega Corporation, #Z6010 and #Z6110), followed by retrotranscription to cDNA using the GoScript™ Reverse Transcriptase kit (Promega Corporation ...
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bioRxiv - Cell Biology 2024Quote: ... HRP conjugated anti-rabbit (W401B; WB: 1:10,000) and anti-mouse (W402B; WB: 1:10,000) secondary antibodies were from Promega. Goat anti-Mouse IgG (H + L ...
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bioRxiv - Cancer Biology 2023Quote: ... HRP Conjugate (cat no. W4021) were purchased from Promega. Goat anti-Rat IgG ...
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bioRxiv - Cancer Biology 2022Quote: ... HRP Conjugate (cat no. W4021) were purchased from Promega.
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bioRxiv - Cell Biology 2020Quote: ... As secondary antibodies we used horseradish peroxidase coupled anti-mouse (Promega W402B), anti-rabbit (Promega ...
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bioRxiv - Genetics 2021Quote: ... the following antibodies were used: mouse anti-beta-galactosidase (Promega; 1:100), mouse anti-Mmp1 (1:1:1 mixture of DSHB 3A6B4 ...
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bioRxiv - Biochemistry 2022Quote: ... membrane was incubated with anti-HaloTag antibody (1:1000; Promega Corporation; G9281) and then with anti-Rabbit IgG Horseradish Peroxidase linked whole antibody (1:5000 ...
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bioRxiv - Immunology 2020Quote: ... IgG were deglycosylated using PNGase F (Promega) with 0.05 U of PNGase/μg of IgG ...
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bioRxiv - Cancer Biology 2023Quote: ... or rabbit IgG (cat. no. W401B; Promega). Protein signals were visualized using an iBright CL1500 Imaging system (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... and bacterial bioluminescence was measured every 24 h for up to 120 h with a GloMax Multi Detection System (Promega, Madison, WI). For the M.tb replication rate experiment ...
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bioRxiv - Neuroscience 2022Quote: ... 10µl RNasin RNase-inhibitor (40U/l, Promega) were added ...
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bioRxiv - Immunology 2020Quote: ... and M-MLV RT RNAse (H-) Point Mutant (Promega) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... and coelenterazine h was purchased from Promega (Madison, WI). 7-ethoxyresorufin was purchased from Anaspec (Fremont ...
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bioRxiv - Biophysics 2021Quote: ... except that the substrate used was coelenterazine h (Promega, 5μM final concentration in drug buffer ...
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bioRxiv - Microbiology 2024Quote: ... and incubated with coelenterazine-h (Promega, Madison, WI, USA) at a final concentration of 2.5 nM ...
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bioRxiv - Cell Biology 2024Quote: ... for 3 h and with trypsin (1:25, Promega) for 16 h both at 37°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 10 µL coelenterazine h (Promega, 5 µM final concentration) was added ...
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bioRxiv - Molecular Biology 2024Quote: ... Plates were then incubated overnight at 4°C with primary antibodies (mouse anti-LgBIT antibody at 1:750 dilution [Promega N7100] ...
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bioRxiv - Plant Biology 2021Quote: ... HaloTag-ligand conjugated magnetic beads (Promega) were used to pull down the HaloTag-fused TFs ...
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bioRxiv - Cell Biology 2023Quote: ... For TMR-conjugated ligand (G825A; Promega) treatment ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with the primary antibodies (1D4 1:1500, Anti-Calnexin Sigma c4731 1:600, Anti-LgBiT Promega N7100 1:100 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CP4 medium was exchanged with Hanks’Balanced Salt Solution (with added glucose 1 g/l, NaHCO3 0.35 g/l) containing GloSensor cAMP Reagent (3 %, Promega) and plates were incubated for 60 min ...
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bioRxiv - Bioengineering 2021Quote: ... At time points of 1 h and 48 h samples were tested for cytotoxicity via a commercially available LDH-Glo™ Cytotoxicity Assay (#J2380, Promega, Madison, WI). In brief ...
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bioRxiv - Cell Biology 2020Quote: ... samples were blocked in 20% normal goat serum (NGS) for 1 h and incubated with the HaloTag® polyclonal anti-rabbit (Promega, 100-fold dilution) in PBS supplemented with 2% NGS overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... Protein production was confirmed by western blot using an anti-HaloTag antibody (Promega). HaloTag-ligand conjugated magnetic beads (Promega ...
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bioRxiv - Microbiology 2021Quote: ... Immunodetection was carried out using rabbit polyclonal anti-Halo antibody (Promega; 1:1000), mouse anti-HA antibody (Roche ...
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bioRxiv - Developmental Biology 2021Quote: The antibodies used were: Rabbit anti-pJNK pTPpY (1:500, Promega, Cat#V93B), Rat anti-Ci(1:1,000 ...
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bioRxiv - Neuroscience 2023Quote: ... The following antibodies were used for immuohistochemistry: mouse anti-β-gal (Z3781, Promega), chicken anti-NeuN (266006 ...
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bioRxiv - Developmental Biology 2023Quote: ... and rabbit anti-Caspase-3 polyclonal antibody (1:250, Promega/Fisher, PR-G7481) overnight at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... We used the following primary antibodies: mouse anti-LacZ (1:500 dilution, Promega) rabbit polyclonal anti- cleaved Caspase 3 (1:200 dilution ...
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bioRxiv - Neuroscience 2024Quote: ... The following antibodies were used: mouse anti–βIII-tubulin (1:2,000; Promega, G7121) and rabbit anti-synaptotagmin-2 (1:500 ...
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bioRxiv - Synthetic Biology 2024Quote: ... The anti-HaloTag antibody, (G921A, 1:500 for WB) was purchased from Promega. The Alexa Fluor™ 488 Goat anti-Mouse IgG (A11029 ...
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Microbial iCLIP2: Enhanced mapping of RNA-Protein interaction by promoting protein and RNA stabilitybioRxiv - Molecular Biology 2024Quote: ... while α-mouse HRP conjugate (1: 5000 dilution; Promega; W4021) was used as the secondary antibody ...
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bioRxiv - Cell Biology 2024Quote: ... The resulting pellets were resuspended in digestion buffer (1 M Guanidinium Chloride in 100 mM HEPES pH 8.0) and digested by consecutive addition of LysC (3 h at 37°C) and trypsin (Promega, 16 h at 37°C). The obtained digested peptides were acidified and desalted using a Waters Oasis HLB μElution Plate 30 μm (Waters ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed twice with TPBS and incubated for 1h at room temperature with the secondary antibody conjugated with horseradish peroxidase (Promega, #W401B, 1:5,000 dilution) followed by three more washes ...
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bioRxiv - Developmental Biology 2022Quote: ... cDNA was obtained using MMLV H Minus Reverse Transcriptase (Promega) with a dT17 primer ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... followed by 3 h at 37°C using trypsin (Promega). All proteolytic digests were acidified to pH 2 by addition of 10% formic acid and directly analyzed by LC-ESI-MS/MS ...
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bioRxiv - Molecular Biology 2021Quote: ... and reverse transcribed using M-MLV RNase H minus (Promega). cDNA products were purified on gel and amplified by PCR using the Phusion polymerase (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... 24 h later cells were transfected with Fugene HD (Promega) and constructs containing dCas9-VPR and gRNA vectors targeting the conserved DAR region (500ng plasmid DNA in molar ratios sgRNA:dCas9-VPR) ...