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Citations for Promega :
451 - 500 of 5313 citations for 6 Bromo 3 3H imidazol 4 ylmethylene 1 3 dihydro indol 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and pMRXIH together with 3×FLAG tag or HaloTag7 (N2701, Promega, Madison, WI). DNA fragments encoding ATG2A (NM_015104.3 ...
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bioRxiv - Cancer Biology 2023Quote: ... and plates were incubated for 3 days after which CellTiterGlo (Promega, Madison, WI) was added according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell viability and caspase 3/7 activity was measured with CellTiter Glo (Promega) or Caspase Glo (Promega ...
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bioRxiv - Biochemistry 2023Quote: ... RNA (3 μg) was retro-transcribed by using M-MLV (Promega, Madison, WI). qPCR was performed in triplicate by using validated qPCR primers (BLAST) ...
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bioRxiv - Physiology 2023Quote: ... 3 mg of RNA was reverse-transcribed with M-MLV (Promega, Madison, WI). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2023Quote: ... After 3-minute incubation we added 30 µl of NanoGlo Luciferase substrate (Promega) per well and nano luciferase activity was measured by Centro XS3 LB 960 luminometer (Berthold Technologies).
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bioRxiv - Cancer Biology 2023Quote: ... or the Caspase Glo® 3/7 Assay (Promega Corporation, Madison, WI, USA) following the manufacturer’s protocol and plates were read on an EnVision Multilabel Plate Reader (Perkin Elmer ...
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bioRxiv - Cancer Biology 2023Quote: ... and apoptosis was determined using the Caspase 3/7 Glo Assay (G8090, Promega) measuring luminescence with a BioTek Synergy 2 (BioTek/Agilent ...
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bioRxiv - Cancer Biology 2020Quote: ... 5 and 6 d using a CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS) (G3582; Promega, Madison, WI). Cells were incubated with the MTS reagent 3-4 h ...
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bioRxiv - Biochemistry 2024Quote: ... were transfected with 2 µg of bacmid using Fugene 6 transfection reagent (Promega), as described by the manufacturer ...
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bioRxiv - Biochemistry 2021Quote: ... free thiols were alkylated by iodoacetamide and proteins were sequentially digested with endoproteinase Lys-C (Wako, 1:100 enzyme-to-substrate ratio, 3 h at 37 °C) and trypsin (Promega, 1:50, overnight at 37 °C). Digested samples were desalted ...
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bioRxiv - Microbiology 2019Quote: ... For transfection 0.5 or 2 µg of plasmid DNA in 25 or 200 µL serum-free medium were mixed with 1 or 4 µL of FuGENE HD transfection reagent (DNA to reagent ratio of 1:2, Promega, Mannheim, Germany) and incubated for 10 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... DNA:FuGENE complexes were formed at a ratio of 1:3 (μg DNA/μL FuGENE HD) according to the manufacturer’s protocol (Promega, Madison, WI, USA). The resulting transfection complex (1 part ...
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bioRxiv - Microbiology 2019Quote: ... The next day they were transfected over 24 hours with a DNA-Fugene HD mixture at a ratio of 1 μg DNA to 3 μl Fugene (Promega, Southampton, UK) according to the manufacturer’s instructions (Western analysis ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were transfected with 1 µg of DNA diluted in 45 µl dMEM and then mixed with 3 µl ViaFect™ (Promega, USA) transfection reagent added and incubate for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... approximately 200,000 HEK-293T cells were transfected with 1 μg of plasmid DNA complexed to 3 μL of FuGENE HD (Promega, Cat: E2311). 16-20 hours post transfection the cells were dissociated from the plastic using cell dissociation buffer (Gibco Cat ...
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bioRxiv - Microbiology 2022Quote: ... The cell suspension was mixed 1:1 with ONE-GloTM Luciferse Assay Buffer (Promega), and the luminescence was measured with the Synergy HTX Multi-Mode Reader ...
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bioRxiv - Microbiology 2020Quote: ... The beads were then washed 3 times and incubated with 30ul lysis buffer (Promega). Then 15μl of the lysate was used to measure bound NanoLuc activity.
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bioRxiv - Cell Biology 2020Quote: ... Caspase 3/7 activity was measured using an Apolive-Glo kit (Promega, Madison, WI) and measured using a SpectraMax M5 (Molecular Devices ...
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bioRxiv - Biochemistry 2020Quote: ... and were harvested on day 3 by scraping into passive cell lysis buffer (Promega). Luciferase activity was then measured using a commercial dual luciferase assay kit (Promega) ...
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bioRxiv - Physiology 2020Quote: ... caspase 3/7 activity was measured using ApoLive-Glo Multiplex Assay (Promega, Madison, WI). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... Each sample was cut into 3 slices and in-gel digested with trypsin (Promega) as previously described15 ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were digested overnight at 37 °C by addition of 3 μg trypsin (Promega) and 2 μg LysC (Promega) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell apoptosis was assessed using either the CaspaseGlo 3/7 assay (G8090, Promega, UK) according to the manufacturer’s instructions (N=4 replicates) ...
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bioRxiv - Evolutionary Biology 2022Quote: Effector caspase activity was assessed using the Caspase-Glo 3/7 Assay System (Promega) following a previous protocol (Ronai et al. ...
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bioRxiv - Cancer Biology 2022Quote: ... an equal volume of Caspase-Glo® 3/7 Assay System (Cat. No.G8090, Promega) was transferred into each well and incubated for 1 h and measured by a GloMax® 20/20 Luminometer ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and caspase-3/7 activity were measured using the ApoTox-Glo Triplex Assay (Promega) in a GloMax-Multi+ Reader (Promega) ...
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bioRxiv - Cancer Biology 2021Quote: ... Apoptotic activity was determined by the Caspase-Glo 3/7 luminescent assay (Promega, G8092), Annexin-V staining (Roche ...
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bioRxiv - Bioengineering 2020Quote: ... Cell apoptosis was measured using a Caspase-Glo 3/7 assay (Promega, Madison, WI).
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bioRxiv - Immunology 2020Quote: For cloning the USP18 3’UTR in the psiCHECK2 vector (Promega, Madison, WI, USA), the first 559nt of USP18 3’UTR were amplified from cDNA of HeLa S3 treated with IFN using the primers USP183UTR_XhoI_FW and USP183UTR_NotI_RV ...
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bioRxiv - Cancer Biology 2023Quote: ... Viability of organoids was determined after 3 days using CellTiterGlo reagent (Cat #G9683, Promega) and detected using a FluoStar Optima Plate Reader (21999 ...
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bioRxiv - Cancer Biology 2023Quote: ... survival was assessed using Cell Titer-Glo and Caspase 3/7 Glo assays (Promega) and analyzed at 24–48h post-treatments ...
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bioRxiv - Cell Biology 2023Quote: Caspase activity was assessed using Caspase-3/-7 Glo Luminescent Assay (Promega, Madison, WI) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... and an apoptosis assay using Caspase-Glo® 3/7 reagent (G8091, Promega, Canada), as per manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... 0.006 uM and cell viability was measured after 3 days using CTG-3D (Promega). To test the response to a fixed concentration of cisplatin 20,000 cells were seeded in a 12-well plate using 3 replicates per condition ...
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bioRxiv - Cancer Biology 2023Quote: Apoptosis was assessed using the luminescence-base Caspase-Glo 3/7 assay kit (Promega) and defined by at least a 1.5-fold increase in signal activation concerning controls ...
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bioRxiv - Neuroscience 2024Quote: ... the Caspase-Glo® 3/7 assay was performed according to manufacturer’s instructions (Promega) on HEK cells after 40 h treatment with cisplatin ...
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bioRxiv - Cancer Biology 2024Quote: Apoptosis was assessed by Caspase-Glo 3/7 assay according to manufacturer’s instructions (Promega). A total of 10,000 cells/well were grown in 24-well clear plates and treated with 5.5 µg (in vivo concentration ...
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bioRxiv - Cancer Biology 2024Quote: ... Tumoral growth was followed by bioluminescence upon injection of 3 mg luciferin-EF (Promega) using a Photon Imager device (Biospace Lab) ...
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bioRxiv - Biophysics 2024Quote: ... LgBiT and linker (5’-GGGAGTTCCGGTGGTGGCGGGAGCGGAGGTGGAGGCTCGAGCGGT-3’) inserted into the HaloTag-removed-pFC15A vector (Promega). GPCR sequences were obtained from PRESTO-tango kit (Addgene Kit #1000000068) ...
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bioRxiv - Molecular Biology 2021Quote: ... FASP was carried out in two 10 kDa MWCO filters with a 50 mM iodoacetamide alkylation step and proteins were digested in 2M urea with 2% wt/wt Lys-C (Wako) for 6 h and 2% modified trypsin (Promega) for 12 h at 37°C ...
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bioRxiv - Physiology 2021Quote: ... allowing measurement of 2-deoxyglucose-6-phosphate using the Glucose Uptake-Glo assay (Promega) and a FLUOstar Omega luminescence plate reader.
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bioRxiv - Immunology 2021Quote: ... and 1 μg shRNA plasmid using Fugene 6 (Promega). The next day ...
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bioRxiv - Biochemistry 2023Quote: ... 30 μL of CellTiter Glo (Promega, diluted 1:6), was added to each well and luminescence was measured with an Envision plate reader ...
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bioRxiv - Microbiology 2020Quote: ... followed by a reverse transcription using a universal degenerated oligo specific to all 5′ non-coding sequences of BTV-1 segments (BTV/Uni1; 5′ GTTAAAWHDB 3′) and the GoScript Reverse Transcription (RT) System (Promega, Madison, WI, USA). The cDNAs obtained for the segment 7 were then quantified with a real time quantitative PCR (qPCR) ...
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bioRxiv - Systems Biology 2023Quote: ... at the ratio of 1:50 for 3 h at 37°C and then using trypsin (sequencing grade modified trypsin; Promega, Fitchburg, WI, USA) at the ratio of 1:50 at 37°C overnight (for 15 h to 18 h ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were examined for viability every 4 to 6 weeks with the CellTiter-Glo assay (Promega). Cell viability was measured in quadruplicates by seeding the cells (2,000 to 3,000 per well in 96-well plate) ...
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bioRxiv - Cell Biology 2023Quote: ... then transfected with 4 μg pLZRS-GCaMP6 DNA plus 12 μl FuGENE 6 (Promega Cat. #E2691) in 800 μl of Opti-MEM (Thermo-Fisher Cat ...
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bioRxiv - Genomics 2020Quote: ... 4 µL RNase-A (4 mg-mL-1, Promega, Madison WI) was incubated at 37 °C for one hour vortexing every 15 minutes ...
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bioRxiv - Molecular Biology 2020Quote: ... RNase One (Promega) (5 units per ug of RNA ...