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Citations for Promega :
451 - 476 of 476 citations for 4 4' Di N acetylamino diphenylsulfone d8 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 1,200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends in pCI mammalian expression vector (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (11668019 ...
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bioRxiv - Biochemistry 2024Quote: ... HEK293T cells were transfected with 3.5μg of N-terminally FLAG-tagged receptor and 3.5μg of an SRE-based luciferase reporter plasmid pGL4.33 (Promega, Cat. no: E1340). 14-16h after transfection ...
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bioRxiv - Microbiology 2022Quote: In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously [25] ...
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bioRxiv - Microbiology 2022Quote: In vitro transcription was performed for EagI-cleaved YACs and PCR-amplified SARS-CoV-2 N gene using the T7 RiboMAX Large Scale RNA production system (Promega) as described previously20 ...
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bioRxiv - Biochemistry 2022Quote: ... 1200 ng of ORs tagged with the first 20 amino acids of human rhodopsin (rho-tag) at the N-terminal ends53 in pCI (Promega) and 30 ng eGFP were transfected using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... The amplified DNA fragments were purified using Wizard SV Gel and the PCR Clean-Up System (Cat. N° A9281 Promega), then the DNA was sequenced using the Macrogen facility (https://dna.macrogen.com/).
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bioRxiv - Microbiology 2024Quote: ... The 25 μl reaction contained 5 μl of PCR Green Buffer with magnesium chloride (Promega, Cat N° M7845, Madison, USA), 1 μl of 5 mM dNTP ...
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bioRxiv - Evolutionary Biology 2024Quote: ... on the 12th day (n=3 individuals/ dietary treatment/sex) and isolated microbial DNA using a Wizard genomic DNA isolation kit (Promega) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and MSN(T558A) genes with either N-terminal or C-terminal SmBit and LgBiT fusions were produced using Flexi®-based cloning (Promega). Best luminescence signals were obtained with CD44 containing a C-terminal smBiT fusion (C-terminal smBiT ...
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bioRxiv - Molecular Biology 2023Quote: Plasmids used as DNA template in PCR reactions to generate donor DNAs for gene deletion/disruption strategies were obtained by amplifying blasticidin S deaminase (BSD) and puromycin-N-acetyltransferase (PAC) genes and cloning them individually into pGEM®-T Easy Vector (Promega). The reverse primers to amplify both genes included GTGA as four last nucleotides ...
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bioRxiv - Cell Biology 2023Quote: ... proteins were generated with pCS2-N-terminal 5×MYC vectors and pCS2-N-terminal 3×HA vectors described above using TnT® Coupled Reticulocyte Lysate System under the SP6 promoter (L4600, Promega) and by following manufacturer’s recommendations with few modifications ...
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bioRxiv - Pathology 2024Quote: ... followed by dilution to 1 M urea with 25 mM AmBic and 37 °C o/n digestion with trypsin protease (MS grade; Promega, V5280) in protein/protease ratio of 40:1 (w/w) ...
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bioRxiv - Biochemistry 2021Quote: ... which contains a pRSET backbone with an N-terminal 6xHis tag and the insertion of a biotin tag from the PinPoint™ Xa-1 Vector (Promega, USA) in between the His tag and the first fluorescent protein ...
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bioRxiv - Cell Biology 2020Quote: To compare Promega’s NanoBiT system β-arrestin2 was inserted at the C-terminal end of LgBiT using Promegas pBiT1.1-N vector (Promega Cat. #N2014) and the doubly palmitolyated fragment of GAP43 was inserted at the N-terminal of SmBiT using the pBiT2.1-C vector.
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bioRxiv - Microbiology 2020Quote: ... Proteins were digested O/N at 37°C with mass spectrometry grade lysyl endopeptidase LysC and sequencing grade modified trypsin (Promega LTD, UK).
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bioRxiv - Genomics 2021Quote: ... were transfected at 20 μM in SK-N-SH cells at a density of 104 -105 cells using FuGene HD Transfection reagent (Promega Corporation, USA) per the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... 1ug of total RNA extracted from transfected SK-N-MC cells was reverse transcribed using the ImProm-II Reverse Transcription System and random hexamer primers (Promega, Madison, MI). PowerUp SYBR™ Green Master Mix (Thermo Fisher ...
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bioRxiv - Biophysics 2022Quote: pHalo-PPARγ2 expresses human PPARγ isoform 2 fused to HaloTag in the N-terminus under a CMVd1 promoter (Promega ORF clone #FHC08305). PPARγ2 mutants were generated by nucleotide substitution using the QuikChange II XL Site Directed Mutagenesis Kit (Stratagene ...
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bioRxiv - Genetics 2022Quote: ... were transfected at 25 µM concentration in SK-N-SH cells at a density of 104 -105 cells using FuGene HD Transfection reagent (Promega Corporation, USA), per the manufacturer’s instructions ...
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bioRxiv - Biophysics 2023Quote: ... Halo-EGFR constructs used for FRET-FLIM experiments were created using the HA-EGFR plasmid and the Gibson assembly approach to replace the N-terminal HA tag with the HaloTag® plasmid (Promega, #G7711). The final resulting construct contained the HaloTag and a GSGS linker region after the EGFR signal sequence ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids with the arsC-1Fas and arsC-2Fas correct sequences were purified by miniprep (Wizard® Plus SV Minipreps, Promega, cat. n° A1360) and double digested with XhoI and HindIII (Thermo Scientific™ ...
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bioRxiv - Molecular Biology 2021Quote: ... Larvae with the same genotype were pooled (min. n=10 larvae/sample) and RNA was extracted using the ReliaPrep RNA Miniprep System (Promega AG, Dübendorf, Switzerland) following manufacturer’s user guide ...
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bioRxiv - Cell Biology 2021Quote: HEK293 T-REx SNAPf-GLP-1R-SmBiT cells were seeded in 12-well plates and co-transfected with 0.1 μg β-arrestin-2 fused at the N-terminus to LgBiT (LgBiT-β-arrestin-2; Promega, plasmid no. CS1603B118) and either 0.1 μg mini-Gs-Venus or mVenus supplemented with 0.8 μg pcDNA 3.1.
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bioRxiv - Neuroscience 2022Quote: RNA was extracted from sections of frozen mouse brain cerebrum representing each treatment group (n = 6 per group) using the Maxwell 16 LEV simplyRNA Tissue Kit (Promega, Ipswich, MA, USA; AS1270). RNA was extracted from cultured cells using the Maxwell 16 Cell LEV Total RNA Purification Kit (Promega ...
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bioRxiv - Microbiology 2022Quote: ... The purified PCR products were subjected to A-tailing with Taq DNA polymerase and to ligation into a T-vector (pGEM®-T Easy Vector, Promega, cat. n° A1360). The ligation product was used to transform E ...
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bioRxiv - Synthetic Biology 2023Quote: Casp3-containing matrix consisted of an N- and C-terminally HaloTag®-ed Casp3 coupled to Magne® HaloTag beads (Promega, Cat.-No. G7281). To ensure binding on both termini ...