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Citations for Protein Simple :
151 - 200 of 206 citations for Recombinant Human TNFRSF17 protein Fc His tagged APC labeled since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... anti-Mouse Detection module (DM-002) and anti- Rabbit Detection module (DM-001) from Protein Simple were used with specific primary antibodies ...
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bioRxiv - Genetics 2021Quote: ... and their respective secondary antibodies in the 12-230 KDa capillary cartridge separation module (Protein Simple) as per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative analysis of the CFTR B and C-bands was performed using Compass software (Protein Simple).
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bioRxiv - Cancer Biology 2022Quote: ... Tissue homogenate samples were lysed with Bicine/CHAPS Lysis Buffer (Protein Simple, San Jose, CA, USA) containing phosphatase and protease inhibitors ...
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bioRxiv - Microbiology 2020Quote: ... the chemiluminescent revelations of the ladder and samples were established with peroxide/luminol-S (Protein Simple). The digital image was analysed with Compass for SW software (v 4.1.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative analysis of the CFTR B and C-bands was performed using Compass software (Protein Simple).
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bioRxiv - Neuroscience 2021Quote: ... Samples (0.3 ug) were loaded to each lane into the Wes™ assay plate (Protein Simple), and 12 to 230 kDa separation modules were used ...
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bioRxiv - Immunology 2021Quote: ... IL-10 and TNFα were measured with the Ella platform (Protein Simple, San Jose, CA, USA) using multiplex cartridges ...
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bioRxiv - Cancer Biology 2020Quote: ... and immunoblot and electrophoretograms were composed and analyzed by Compass Software (Protein Simple, San Jose, CA). All lanes shown together in western data derive from the same experiment and were processed in parallel.
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bioRxiv - Neuroscience 2022Quote: ... Quantitative analysis was performed using the Compass software (Protein Simple, Bio-Techne, San Jose, CA, USA) and data expressed as fold change relative to control group..
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bioRxiv - Biochemistry 2023Quote: Microsomes were diluted to 0.5 mg/mL in 0.1X Sample Buffer (Protein Simple, San Jose, CA) and subjected to WESTM capillary system analysis ...
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bioRxiv - Neuroscience 2024Quote: ... Immunoreactive bands were visualized through detection of chemiluminescence using a CCD camera imager (FluoroChemM, Protein Simple). Quantification of signal intensity in the various bands was performed using the standard Fiji software package.
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bioRxiv - Neuroscience 2024Quote: ... and the immunoblot signal was captured using an imaging system (FluorChem E, Protein Simple, California, USA).
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bioRxiv - Pathology 2024Quote: ... Gel documentation was performed using an Alpha Imager Gel Documentation System (Model: Mini, Protein Simple, USA) for visualization and image capture.
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bioRxiv - Cell Biology 2020Quote: ... Antigen-antibody complexes were visualized by chemiluminescence using a FluorChem FC2 system (Protein Simple, San Jose, CA) and Super Signal West Dura (Thermo Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... Cell lysates were prepared and diluted to 3 mg/ml using the sample preparation kit (Protein Simple) for an automated capillary western blot system ...
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bioRxiv - Biophysics 2019Quote: ... The stained gel and immune blot images were acquired with a FluorChem Imager instrument (Protein Simple, CA).
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bioRxiv - Neuroscience 2022Quote: ... All membranes were then washed four times in TBST and imaged on the FluorChem-E (Protein Simple) or the ChemiDoc MP (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... Imaging of the membranes was performed using the Fluorchem M imaging system (Protein Simple, San Jose, CA), and densitometry was performed using ImageJ.
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bioRxiv - Synthetic Biology 2023Quote: ... following the manufacturer’s instructions and detected using a FluorChem M imager (Protein Simple, San Jose, CA, USA) by the Multifluor Red setting ...
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bioRxiv - Cancer Biology 2022Quote: ... and images were captured using an AlphaInnotech Fluorchem Q Imaging System (Protein Simple, San Jose, CA, USA). Selected areas surrounding the predicted molecular weight of the protein of interest were extracted from whole blot images.
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bioRxiv - Cancer Biology 2023Quote: ... and images were captured using an AlphaInnotech Fluorchem Q Imaging System (Protein Simple, San Jose, CA, USA). Selected areas surrounding the predicted molecular weight of the protein of interest were extracted from whole blot images.
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bioRxiv - Cell Biology 2024Quote: ... SallySue or Jess (Biotechne) were used according to manufacturer’s instructions (Protein simple, Biotechne, #SM-S001, #SM-S002). Quantification was performed by normalisation to calnexin or using Simple Western total protein quantification ...
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bioRxiv - Biochemistry 2020Quote: ... we performed the immune assay using the capillary Western-blot system (Wes, Protein Simple; San Jose, CA, USA), due to the sensitivity compared to the traditional western-blot method ...
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bioRxiv - Neuroscience 2021Quote: ... Digital image of chemiluminescence of the capillary was captured with Compass Simple Western software (version 5.1.0, Protein Simple) that calculated automatically heights (chemiluminescence intensity) ...
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bioRxiv - Microbiology 2019Quote: ... Chemiluminescent detection was then performed using supplied detection reagents and visualization performed using FluorChem E imaging system (Protein Simple). See Supplementary Table 1 for plate layout.
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bioRxiv - Microbiology 2020Quote: ... Data analysis and the generation of virtual western blots was done using the Compass Software (Protein Simple, Version XZ).
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bioRxiv - Microbiology 2020Quote: ... PCR products were analyzed via agarose electrophoresis and visualized under UV light in an AlphaImager gel doc (Protein Simple). PCR amplified products were sequenced to confirm specificity.
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bioRxiv - Immunology 2022Quote: ... Digital image of chemiluminescence or fluorescence of the capillary was captured with Compass Simple Western software (version 5.1.0, Protein Simple) that calculated automatically heights (chemiluminescence or fluorescence intensity) ...
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bioRxiv - Molecular Biology 2023Quote: ... The run was performed using default settings and analyzed using the Compass Software for Simple Western (version 5.0.1, Protein Simple).
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bioRxiv - Microbiology 2023Quote: ... Mouse serum samples were diluted 10-fold using the kit-specific reagent (SPCKA-MP-007374, Protein Simple, Bio-Techne), and the concentrations were measured following the manufacturer’s instructions on the Ella system ...
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bioRxiv - Cancer Biology 2022Quote: Simple Western 12–230 kDa size-assays were carried out using Peggy SueTM analyzer (Protein Simple, San Jose, CA, USA). First ...
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bioRxiv - Microbiology 2020Quote: ... to achieve a final concentration of 0.20 μg/μL in the presence of fluorescent molecular weight markers and 400 mM dithiothreitol (DTT, Protein Simple). This preparation was then denatured at 95°C for five minutes ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were either processed using a WES system and transformed to a virtual Western blot (Figure 2A, Protein Simple, Biotechne) or analysed by standard Western blot (Figure 2B).
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bioRxiv - Physiology 2021Quote: ... was applied and the membranes were subsequently imaged using a FluorChem E Chemiluminescence Imager (Protein Simple, San Diego, CA, USA). Analysis of densitometry was completed using AlphaView SA Software ...
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bioRxiv - Neuroscience 2021Quote: ... samples were diluted to 0.1 μg/μL in provided 1× sample buffer and 1× fluorescent molecular weight marker/reducing agent (Protein Simple). Samples were then vortexed ...
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bioRxiv - Microbiology 2021Quote: ... The supercoiled (SC) and nicked circular (NC) forms of plasmid was quantified using the software FluorChem Q (Protein Simple, Inc.). SC ratio was calculated as SC/(SC+NC ...
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bioRxiv - Bioengineering 2024Quote: ... A range of pI markers (pI 3.85-8.77) were used to capture all main and impurity isoforms for each sample (Bio-Teche, Protein Simple, USA). To minimise self-association ...
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bioRxiv - Cancer Biology 2020Quote: ... and 25μg cell line lysates in RIPA buffer were separated by WES automated gel electrophoresis System (Protein Simple, San Jose, CA). Cell lines were procured from authenticated sources ...
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bioRxiv - Microbiology 2021Quote: ... and whole Spike (20 µg/ml)] were covalently fixed with ultraviolet irradiation to a 12-230 kDa Jess & Wes Separation Module (Protein Simple). The immobilized viral components were then exposed to each of 30-fold-diluted convalescent plasma samples (primary antibodies) ...
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bioRxiv - Genomics 2020Quote: Cells were lysed in M-per buffer and concentration of Cas9 was performed using automated Ella assay using Ella instrument (Protein Simple). An aliquot of 5 μL cell lysate or Cas9 standard solution was mixed with 45 μL sample diluent (D-13 ...
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bioRxiv - Microbiology 2019Quote: ... imaged using a white light transilluminator and a CCD camera equipped with an 595 ± 55 nm bandpass filter (Fluorichem 8000; Protein Simple, Inc.), and then quantified using Imagequant software (GE Healthcare).
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bioRxiv - Cell Biology 2019Quote: Protein sample preparation and quantification of p-ERK was performed as previously described 58 using a WES capillary system (12–230 kD Master kit α-Rabbit–HRP; PS-MK01; Protein Simple) and the antibody p-ERK/2(T202/Y204 ...
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bioRxiv - Microbiology 2023Quote: ... Mouse serum samples were diluted 10-fold using the reagent diluent provided with the kit (SPCKA-MP-007374, Protein Simple, Bio-Techne), and the concentrations were determined following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibody was applied for one hour (1:10,000 HRP-goat-anti-rabbit; Jackson Immuno; West Grove, PA) and developed using a FluorChemE imager (Protein Simple; San Jose, CA). Blots were stripped and re-probed using an antibody directed towards GAPDH (1:10,000 ...
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bioRxiv - Pathology 2019Quote: Proteomic results were confirmed by western blotting using the BIORAD system or simple western analysis using the WesTM protocol according to the manufacturer’s (Protein Simple Biosciences & Technology, USA) protocol ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts of protein were prepared for capillary-based (Wes) immunoassay using a 12-230 Separation module kit according to manufacturer’s instructions (Protein Simple, San Jose, CA). The concentration of sample protein and antibodies used in experiments were in the dynamic range of protein level detection and was determined through empirical optimization ...
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bioRxiv - Immunology 2022Quote: ... SPCKB-PS-000502) were measured in EDTA plasma using the Simple PlexTM cartridges run on the EllaTM platform (Protein Simple, San Jose, USA) following the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Chemiluminescence was visualized and quantified by an experimenter blind to the condition of the sample using FluorChem Software (Protein Simple, San Jose, CA). ΔFosB levels were normalized to GAPDH levels to control for equal loading of samples across wells.
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bioRxiv - Genomics 2024Quote: MACS purified erythroid (CD235a+) cells from BM were pooled and 8 x 104 cells were loaded on small scWest chips (Protein Simple, Cat # K500). scWest chips were processed with lysis time for 0 sec ...