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Citations for IBA Life Sciences :
101 - 150 of 267 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Beads were then washed with buffer B and proteins were eluted using buffer B supplemented with 10 mM desthiobiotin (IBA Lifesciences). Samples were taken at each step of the purification and loaded on SDS-PAGE gels to check the efficiency of affinity purification ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20 μL Strep-Tactin Macroprep beads (IBA lifesciences). Beads were washed three times with lysis buffer ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20-30 μl Strep-Tactin Macroprep beads (IBA lifesciences), FLAG or Ni-NTA beads (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleaved-off alkaline phosphatase and TEV protease were removed by re-purifying EXT1-EXT2 using a second HisTrap HP purification step and by passing protein sample through 1 mL Strep-Tactin XT resin (IBA Lifesciences). Buffer was exchanged by several rounds of concentration and dilution with buffer B (20mM HEPES pH 7.2 ...
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bioRxiv - Microbiology 2021Quote: ... The following primary antibodies were used in this study: Strep-Tactin-HRP conjugate (Iba Lifesciences) (dilution 1:3,000 in 3 w/v % BSA/TBS-T) ...
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bioRxiv - Microbiology 2023Quote: ... The following primary antibodies were used in this study: Strep-Tactin-HRP conjugate (Iba Lifesciences) (dilution 1:3,000 in 3 w/v % BSA/TBS-T) ...
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bioRxiv - Molecular Biology 2024Quote: ... and Twin-Strep tagged proteins of interest were purified from crude extracts (soluble fraction) using the Strep-Tactin®XT Superflow® Kit (IBA Lifesciences, Germany). Purification was performed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Samples stained with trimeric SARS-CoV-2 RBD were incubated with primary antibody strepmabHRP (2-1509-001, IBA lifesciences) with specificity towards the TwinStrep-tag at the C-terminus of the trimeric protein for 120 hours in PBSGT with 0.1% saponin ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated with StrepMAB-Classic monoclonal antibody conjugated to HRP (0.05 µg/mL, #2-1509-001, Iba Lifesciences) for 2 h at RT ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: StrepMAB-Immo (anti-Twin-Strep-tag®; IBA Lifesciences; 2-1517-001; 1:4000), FUS (1:2000 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Membranes were rinsed once with PBS-T (PBS with 0.01% Tween-20) and incubated with primary Strep-tag II antibody (Strep-MAB-Classic, IBA Lifesciences, Cat# 2-1507-001 ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated either with anti-strep tag antibody (IBA Lifesciences) or anti-SARS-COV-2 polyclone antibody (Sino Biological Inc. ...
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bioRxiv - Plant Biology 2020Quote: ... They were next incubated in primary antibody solution for 2 hours at room temperature (1:1000 anti-Strep, IBA Life Sciences, 1:10,000 anti-FLAG M2 monoclonal antibody ...
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bioRxiv - Immunology 2023Quote: ... The ELISA plates were washed three times with PBST and then 100 μl of 5,000-fold diluted anti-Strep-Tactin HRP conjugate antibody (IBA Life Science) was added into each well for another 1 h at 37 °C ...
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bioRxiv - Immunology 2023Quote: ... plates were washed three times with PBST and the 5,000-fold diluted anti-Strep-Tactin HRP conjugate antibody (IBA Life Science) was subsequently added ...
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bioRxiv - Immunology 2019Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences # 2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001 ...
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bioRxiv - Microbiology 2022Quote: ... incubated with anti-StrepMAB-Classic-HRP antibody that recognizes the strep tag at the C-terminus of the SFV Env ectodomain (7.5 μg/ml, IBA Lifesciences #2-1509-001) for 1 hour ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were incubated for 1 h at room temperature in blocking solution with mouse StrepMAB antibody (IBA Lifesciences, 1:750, 2-1507-001) for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8 ...
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bioRxiv - Immunology 2023Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences #2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences #2-1507-001 ...
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bioRxiv - Microbiology 2023Quote: ... Lysate was cleared by centrifugation and the supernatant was incubated with a Strep-Tactin Sepharose resin (IBA Lifesciences). Resin was thoroughly washed with lysis buffer and 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2020Quote: ... pASK-IBA7+ was from IBA Life Sciences, pET-Duet1 from Merck KgaA ...
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bioRxiv - Biophysics 2019Quote: ... Strep-Tactin resin was acquired from Iba lifesciences and Nickel resins were purchased from ThermoFisher Scientific (HisPur™ Ni-NTA resin ...
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bioRxiv - Synthetic Biology 2019Quote: ... The supernatant was loaded on a StrepTactinXT (IBA Lifesciences) column with a column volume of 2 mL equilibrated in 100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2020Quote: ... StrepMAB-classic was purchased from IBA Lifesciences (Göttingen, Germany).
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bioRxiv - Microbiology 2021Quote: ... The purification was performed using StrepTactin resin (IBA LifeSciences) and size exclusion chromatography (SEC ...
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bioRxiv - Biochemistry 2022Quote: ... was transformed with a pASK-IBA63plus vector (IBA Lifesciences) harbouring a wild type PheDH strep tag fusion construct (Gielen et al.14 ...
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bioRxiv - Biochemistry 2023Quote: ... H2A.S2/H2B was added to Streptactin resin (IBA Lifesciences) pre-equilibrated in egg lysis buffer (ELB ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was incubated with Strep-Tactin Sepharose (IBA Lifesciences) with gentle shaking for 2 hours at 4 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was loaded on Strep-Tactin (IBA life sciences) affinity chromatography beads in buffer containing Tris-HCl pH 7.5 ...
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bioRxiv - Cell Biology 2019Quote: ... clarified lysate was cycled over a Strep column (IBA Lifesciences) for 2 hr at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was loaded on Strep-Tactin (IBA life sciences) or Ni-NTA (Sigma ...
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bioRxiv - Biochemistry 2020Quote: ... the supernatant was loaded on a strep-tactin (IBA Lifesciences, Germany) column equilibrated with the lysis buffer ...
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bioRxiv - Immunology 2020Quote: Viral gp33 peptide (gp33-41, KAVYNFATM) was purchased from IBA lifesciences. 1 μg /mL was used for re-stimulation of BM CD8+ T lymphocytes along with PMA ...
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bioRxiv - Biophysics 2019Quote: ... The lysate was passaged on a Streptactin XT resin (IBA lifesciences) equilibrated with lysis buffer ...
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bioRxiv - Biophysics 2021Quote: ... Dual-labelled stem loop RNA was purchased from IBA Life Sciences.
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bioRxiv - Microbiology 2022Quote: ... and spike was purified using Strep-Tactin XT beads (IBA Lifesciences), followed by size exclusion chromatography (SEC ...
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bioRxiv - Cell Biology 2023Quote: Affinity purification was performed with MagStrep type3 XT beads (IBA Lifesciences) as recommended by the manufacturer ...
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bioRxiv - Biochemistry 2022Quote: ... Streptactin XT 4F High-Capacity resin was from IBA Life Sciences. [γ-32P]ATP (6,000 Ci/mmol ...
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bioRxiv - Biochemistry 2022Quote: Gene 8 was cloned into pASG-IBA105 (IBA Lifesciences, Götingen, Germany) using the direct cloning method described in the StarGate® direct transfer cloning protocol (IBA Lifesciences ...
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bioRxiv - Biophysics 2022Quote: ... The supernatant was loaded on Streptactin Superflow affinity resin (IBA Lifesciences) and incubated for 2 h under gentle agitation ...
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bioRxiv - Cell Biology 2023Quote: ... Lysate was loaded on Streptactin-XT resin (IBA Lifesciences, Göttingen, Germany) (bead volume ...
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bioRxiv - Biophysics 2023Quote: The solubilized fraction was incubated with Strep-Tactin beads (IBA Lifesciences) for 1 h ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was loaded onto a Strep-Tactin Sepharose (IBA Lifesciences) column equilibrated with 50 mM TRIS ...
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bioRxiv - Cell Biology 2023Quote: Lysate was loaded on Streptactin-XT resin (IBA Lifesciences, Göttingen, Germany) (bead volume ...
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bioRxiv - Molecular Biology 2024Quote: Purification was performed according to the manufacturer’s instructions (IBA Lifesciences, Germany). Briefly ...
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bioRxiv - Biochemistry 2024Quote: ... Affinity chromatography was performed with Strep-TactinTMXT 4FlowTM resin (IBA Lifesciences), and the protein eluted with 100 mM Tris/HCl buffer containing 150 mM NaCl and 50 mM biotin ...
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bioRxiv - Biochemistry 2024Quote: ... Lysate was loaded on Streptactin-XT resin (IBA Lifesciences, Göttingen, Germany) (bead volume ...
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bioRxiv - Biophysics 2024Quote: ... The supernatant was added to Strep-Tactin Sepharose beads (IBA Lifesciences) preequilibrated with Membrane Resuspension Buffer (100 mM Tris-HCl pH 8.0 ...
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Structural investigation of ACE2 dependent disassembly of the trimeric SARS-CoV-2 Spike glycoproteinbioRxiv - Biophysics 2020Quote: ... The transparent supernatant was passed over a Strep-Tactin column (IBA Lifesciences) and bound protein was eluted with PBS buffer + 2.5 mM desthiobiotin ...