Labshake search
Citations for IBA Life Sciences :
51 - 100 of 122 citations for Thioredoxin Domain Containing Protein 3 TXNDC3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... The matrix-bound protein was washed with 15 column volumes (CV) of WB and eluted with 2xCV of WB containing 2,5 μM desthiobiotin (IBA Lifesciences). The eluate was concentrated and subjected to size exclusion chromatography on Superdex 200 increase semipreparative column (10/300 ...
-
bioRxiv - Biochemistry 2022Quote: ... the fractions eluting from the HisTrap column containing myosin were loaded on a Strep-Tactin gravity flow column (IBA Lifesciences) equilibrated with 100 mM Tris pH 8.0 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2xStrep tagged SARS-CoV-2 ORFs were immunoprecipitated using 30 μl of Megastrep type 3 XT beads (IBA LifeSciences, Germany), and flag-tagged SARS-CoV-2 ORFs were immunoprecipitated using Anti-DYKDDDDK Magnetic Agarose (Pierce ...
-
bioRxiv - Molecular Biology 2023Quote: ... The supernatant was filtered through a 5.0 µm syringe filter followed by 0.45 µm syringe filters and loaded on to 3 mL gravity flow column packed with StrepTactin Sepharose resin (IBA Lifesciences). The column was washed with wash buffer (20 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Cell Biology 2021Quote: ... Protein extracts were clarified and incubated with Strep-tactin® Superflow® resin (Iba Life science) at 4°C for 1 hour ...
-
bioRxiv - Microbiology 2021Quote: ... Strep-tagged proteins were purified using affinity chromatography and Strep-Tactin® Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins were expressed in BL21(DE3) and purified by Streptactin-XT resin (IBA Lifesciences, Göttingen, Germany) in the case of homodimers and Streptactin-XT resin and TALON resin (Takara Bio Inc. ...
-
bioRxiv - Microbiology 2022Quote: ... Bound proteins were eluted for 30 minutes using 30 μL of BXT elution buffer (IBA Lifesciences) with constant shaking at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... The HA protein was purified from supernatants using StrepTactin-XT 4FLOW gravity flow columns (IBA Lifesciences). The protein was incubated with BXT elution buffer for 4 h ...
-
bioRxiv - Microbiology 2023Quote: ... The strep tagged protein was purified using affinity chromatography and Strep-Tactin Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... The bound proteins were eluted with the binding buffer supplemented with 10 mM desthiobiotin (IBA Lifesciences). The samples were analyzed by SDS-PAGE and detected by immunoblotting using antibodies to the strep tag (1:3,000 ...
-
bioRxiv - Biochemistry 2022Quote: ... For that purpose VI23.60 containing Strep-tag® was immobilized on the Strep-Tactin® coated microplate (IBA Lifesciences GmbH, Germany) and three rounds of selection were performed ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The lysates were clarified by centrifugation at 30 000 g for 30 min and the supernatant was loaded onto a gravity-flow column containing StrepTactin XT 4Flow resin (IBA Lifesciences, Göttingen ...
-
bioRxiv - Biophysics 2022Quote: ... FLAG beads were rinsed with the same buffer for an additional elution which was combined with the initial peptide elution and incubated with MagStrep “type 3” magnetic bead suspension (IBA Lifesciences) for 2h at 4°C ...
-
bioRxiv - Microbiology 2020Quote: ... coli MG1655 genomic DNA with primers 5’ CTC GGT CTC CAA TGA ATA AAT ATC AGG CAG TGA 3’ and 5’ CTC GGT CTC AGC GCT TTA TTT GAC TAA TGA AAA TAG ATC A 3’ and cloned into the Eco31I (BsaI) sites of plasmid pPR-IBA101 (IBA Lifesciences) to yield plasmid pRCLA10 ...
-
bioRxiv - Neuroscience 2021Quote: ... human SARM1 without mitochondria targeting sequence and with N-terminal tandem StrepTag II was expressed in HEK293T cells expressing NRK1 supplemented with NR (Essuman et al., 2017) and purified with MagStrep (Strep-Tactin) type 3 XT beads suspension (IBA Lifesciences). The purified protein attached on beads was incubated with NAD+ (10 μM for Figure 5 B ...
-
bioRxiv - Immunology 2021Quote: ... 3C-digested proteins were loaded into StrepTactin XT 4Flow® 1 mL gravity flow columns (Iba Lifesciences) and collected in the flow-through for quantification ...
-
bioRxiv - Microbiology 2022Quote: ... 2xStrep tagged S-protein was stained using StrepMAB-Classic DY-488 conjugate (IBA Lifesciences, 2-1563-050) and FLAG tagged S-protein was stained using anti-FLAG (clone M2 ...
-
bioRxiv - Biochemistry 2023Quote: ... and the bound proteins were eluted with the binding buffer supplemented with 10 mM desthiobiotin (IBA Lifesciences). The samples were analyzed by SDS-PAGE and detected by Coomassie staining.
-
bioRxiv - Molecular Biology 2024Quote: ... jejuni 81-116) and Abu0127 proteins were purified according to the Strep-Tactin manufacturer’s protocol (IBA Lifesciences). Briefly ...
-
bioRxiv - Molecular Biology 2022Quote: ... using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences). Subsequently ...
-
bioRxiv - Immunology 2019Quote: ... or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001) and anti-mouse IgG1 antibody Alexa Fluor 647 (Thermo Fisher Scientific # A-21240) ...
-
bioRxiv - Immunology 2023Quote: ... or anti-Strep-tag II antibody (IBA Lifesciences #2-1507-001) and anti-mouse IgG1 antibody Alexa Fluor 647 (Thermo Fisher Scientific #A-21240) ...
-
bioRxiv - Plant Biology 2020Quote: ... The resin was then washed 3 times with RIPA buffer and eluted with 1’ Strep-Tactin elution buffer with biotin (IBA Life Sciences). The input ...
-
bioRxiv - Cell Biology 2019Quote: The custom 20-mer oligonucleotide modified with dibenzocyclooctyne (DBCO) (Sequence: 5’ - TCA GTT CAG GAC CCT CGG CT – DBCO – 3’) was purchased from IBA Life Sciences, reconstituted in nuclease free water at a concentration of 600 μM and stored at −20°C ...
-
bioRxiv - Cell Biology 2023Quote: ... 1 mM TCEP) with pre-equilibrated magnetic Strep-Tactin XT beads (240 µl MagStrep type 3 XT bead slurry, IBA life sciences). Following 1 h of binding at room temperature on a rotator ...
-
bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged: The lysate was passed through chromatography columns containing 1.5 ml bed volume of Strep-Tactin® Sepharose® resin (IBA Lifesciences, Germany). Resin was washed with ∼20 resin volumes of 100 mM HEPES ...
-
bioRxiv - Biochemistry 2020Quote: ... Viral proteins were affinity purified from 4mg lysates by tumbling with 40µl Strep-Tactin Sepharose (IBA Life Sciences) for 90min at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were further purified by loading His60 eluate onto Strep-TactinXT affinity chromatography gravity columns (IBA Lifesciences, Germany). Final pure protein was eluted with Buffer BXT +10% glycerol (IBA Lifesciences ...
-
bioRxiv - Microbiology 2024Quote: ... Spike proteins in culture medium were purified with gravity flow columns using Strep-Tactin XT (IBA Life Sciences), concentrated to 0.5 mg/ml with ultrafiltration tubes (Millipore ...
-
bioRxiv - Molecular Biology 2021Quote: Strep tagged proteins were then purified by incubating whole cell lysates with MagStrep “type3” XT beads (IBA Life Sciences) for five-six hours at 4 °C ...
-
bioRxiv - Cell Biology 2022Quote: ... The Twin-Strep tag and Strep-Tactin XT protein fluorescently conjugated with DY-649 were purchased from IBA Lifesciences.
-
bioRxiv - Biophysics 2022Quote: ... The proteins were purified via a C-terminal STREP II tag using Strep-tactin resin (IBA LifeSciences, Göttingen, DE). The solubilizing detergents were replaced by 0.06% (w/v ...
-
bioRxiv - Molecular Biology 2023Quote: ... the tagged NStr-M35_FL or NStr-M35_S protein were purified by StrepTactin Superflow high capacity (IBA Lifesciences, Göttingen, Germany) chromatography with a 1 mL self-made column (Mobicol ...
-
bioRxiv - Biochemistry 2023Quote: ... and the protein was purified from the supernatant by batch binding to Strep-Tactin XT Superflow resin (IBA Lifesciences). The resin was washed initially with Buffer A containing 0.05 % (w/v ...
-
bioRxiv - Biophysics 2022Quote: ... Mouse monoclonal antibody against the Strep tag (StrepMAB-Classic) was from IBA Lifesciences (Catalog # ...
-
bioRxiv - Microbiology 2019Quote: ... Soluble proteins were loaded on self-prepared columns packed with Strep-Tactin XT superflow or Strep-Tactin Sepharose (IBA lifesciences) and purified under gravity flow ...
-
bioRxiv - Microbiology 2021Quote: ... The protein was expressed and lysed as described above and purified according to the manufacturer’s guidelines (IBA Life Sciences, Inc.). Clarified lysates were passed over Strep-Tactin-Sepharose resin using a gravity filtration column ...
-
bioRxiv - Immunology 2023Quote: ... 200 µg of stabilized histidine tagged S protein were labelled using Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and coated with 1 mg of magnetic beads (Dynabeads His-Tag ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein was captured onto 2 mL of Strep-Tactin Superflow High-Capacity resin (IBA Lifesciences, cat. no. 2-1208-002) equilibrated with HEPES-Ca buffer (20 mM HEPES pH 7.4 ...
-
bioRxiv - Microbiology 2023Quote: ... Concentrated proteins were resuspended in buffer W at pH 8 and incubated with BioLock (#2-0205-050, Iba Life sciences) for 15 min at RT to neutralize biotin ...
-
bioRxiv - Immunology 2024Quote: ... stabilized histidine-tagged SARS-CoV-2 S protein (Wuhan, XBB.1.5, BA.2.86) was labelled with Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and conjugated to magnetic beads according to the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... the proteins were eluted in 1x BXT buffer (150 mM NaCl, 50 mM Tris-HCl pH 8.0, 1mM EDTA; IBA LifeSciences). We then exchanged the buffer to the protein buffer containing 20 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2021Quote: ... The membrane was subsequently incubated with an anti-Strep antibody (StrepMAB-Classic, IBA Lifesciences) at 4°C overnight ...
-
bioRxiv - Microbiology 2022Quote: ... Beads were then washed with buffer B and proteins were eluted using buffer B supplemented with 10 mM desthiobiotin (IBA Lifesciences). Samples were taken at each step of the purification and loaded on SDS-PAGE gels to check the efficiency of affinity purification ...
-
bioRxiv - Biochemistry 2021Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20 μL Strep-Tactin Macroprep beads (IBA lifesciences). Beads were washed three times with lysis buffer ...
-
bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
-
bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
-
bioRxiv - Cell Biology 2022Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20-30 μl Strep-Tactin Macroprep beads (IBA lifesciences), FLAG or Ni-NTA beads (Sigma) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cleaved-off alkaline phosphatase and TEV protease were removed by re-purifying EXT1-EXT2 using a second HisTrap HP purification step and by passing protein sample through 1 mL Strep-Tactin XT resin (IBA Lifesciences). Buffer was exchanged by several rounds of concentration and dilution with buffer B (20mM HEPES pH 7.2 ...