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Citations for IBA Life Sciences :
1 - 50 of 112 citations for Recombinant Human High Mobility Group Box 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2024Quote: ... Strep-tagged proteins were captured on Strep-Tactin XT Superflow High Capacity (IBA LifeSciences) affinity resin ...
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bioRxiv - Molecular Biology 2023Quote: ... the tagged NStr-M35_FL or NStr-M35_S protein were purified by StrepTactin Superflow high capacity (IBA Lifesciences, Göttingen, Germany) chromatography with a 1 mL self-made column (Mobicol ...
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bioRxiv - Biochemistry 2024Quote: ... Affinity purification of Strep3-tagged proteins was performed using a Strep-Tactin® Superflow® high capacity FPLC column (IBA Lifesciences).
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bioRxiv - Biochemistry 2019Quote: Fluorescently tagged Arc and StrepII tagged Arc were coexpressed in HEK293 cells and purified using Strep-Tactin Sepharose (IBA Lifesciences). Formation of complexes between the indicated Arc constructs was analyzed by immunoblotting following SDS electrophoresis ...
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bioRxiv - Immunology 2021Quote: ... or strep-tagged spike immobilized on Strep-Tactin XT magnetic beads (IBA Lifesciences). To select for non-RBD binders ...
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bioRxiv - Biochemistry 2021Quote: ... Twin-Strep-tagged mTOR was captured onto a Strep-Tactin XT (IBA Lifesciences) coated surface prepared according to the supplied instructions ...
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bioRxiv - Immunology 2020Quote: ... Twin Strep- and His6-tagged proteins were captured on Streptactin XT (IBA LifeSciences) or Talon (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... StrepII-tagged proteins were purified using Strep-Tactin MacroPrep (IBA Lifesciences, Göttingen, Germany) following the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... A 1 ml Strep-Tactin XT 4Flow high-capacity cartridge (IBA Lifesciences 2-5027-001) was washed with 14 column volumes of wash buffer (50 mM Hepes pH 7.6 ...
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bioRxiv - Microbiology 2021Quote: ... Strep-tagged proteins were purified using affinity chromatography and Strep-Tactin® Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The strep tagged protein was purified using affinity chromatography and Strep-Tactin Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... twin-strep tagged MKM containing lysates were incubated with Streptactin XT magnetic bead slurry (IBA Lifesciences) and purified according to manufacturer’s recommendation ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM EDTA) and loaded onto a 5 ml Strep-Tactin Superflow High Capacity colum (IBA Lifesciences). Proteins were eluted from the column in Strep buffer supplement with 2.5 mM desthiobiotin followed by over-night TEV cleavage of the N-terminal affinity tag for 16 h at 4 °C during dialysis against Strep buffer ...
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bioRxiv - Genetics 2021Quote: ... Strep-tagged Orf8 complexes were eluted from beads in Buffer BXT (IBA Lifesciences; Cat. # 2-1042-025) shaking at 1100 rpm for 30 min.
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bioRxiv - Genetics 2021Quote: ... Strep-tagged Orf8 complexes were eluted from beads in Buffer BXT (IBA Lifesciences; Cat. # 2-1042-025) shaking at 1100 rpm for 30 min.
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bioRxiv - Microbiology 2022Quote: ... 2xStrep tagged S-protein was stained using StrepMAB-Classic DY-488 conjugate (IBA Lifesciences, 2-1563-050) and FLAG tagged S-protein was stained using anti-FLAG (clone M2 ...
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bioRxiv - Immunology 2023Quote: ... TwinStrep tagged 8.9F was purified via gravity column before being eluted by 1X BXT (IBA Life Sciences) while 6x-His tagged 8.9F and 18.5C culture supernatants were rolled overnight with Ni-NTA beads at 4°C before using gravity columns to capture the resin ...
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bioRxiv - Molecular Biology 2021Quote: Strep tagged proteins were then purified by incubating whole cell lysates with MagStrep “type3” XT beads (IBA Life Sciences) for five-six hours at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... the tagged d1_d2 was purified from the supernatant by affinity chromatography using Strep-Tactin resin (IBA Lifesciences, Göttingen, Germany).
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bioRxiv - Molecular Biology 2021Quote: ... 2xStrep tagged SARS-CoV-2 ORFs were immunoprecipitated using 30 μl of Megastrep type 3 XT beads (IBA LifeSciences, Germany), and flag-tagged SARS-CoV-2 ORFs were immunoprecipitated using Anti-DYKDDDDK Magnetic Agarose (Pierce ...
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bioRxiv - Immunology 2023Quote: ... 200 µg of stabilized histidine tagged S protein were labelled using Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and coated with 1 mg of magnetic beads (Dynabeads His-Tag ...
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bioRxiv - Immunology 2024Quote: ... and recombinant proteins were purified using Strep-Tactin XT resin (IBA Lifesciences, Cat. #2-5010), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore ...
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bioRxiv - Biophysics 2023Quote: ... Supernatants were incubated with end-over-end rotation for 1 hour at 4°C with Strep-Tactin Superflow high-capacity resin (IBA Lifesciences) equilibrated with gel-filtration buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... was used to produce twin-strep-tagged fusion chemokines which were detected with an HRP-conjugated anti-Streptag mAb (IBA LifeSciences). Plates were developed with TMB One Component (Surmodics ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
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bioRxiv - Molecular Biology 2023Quote: ... or Strep-Tactin Superflow high capacity resin (IBA Lifesciences) and incubated with rotation at 4°C for 1 h ...
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bioRxiv - Synthetic Biology 2022Quote: ... The solution was then centrifuged 3 times at 20,000 × g at 4 °C for 10 min periods and the supernatant was used for purification using fast protein liquid chromatography (FPLC) and 1 mL Strep-Tactin Superflow high capacity column (IBA Lifesciences, Germany). After equilibration with 5 column volume (CV ...
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bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged: The lysate was passed through chromatography columns containing 1.5 ml bed volume of Strep-Tactin® Sepharose® resin (IBA Lifesciences, Germany). Resin was washed with ∼20 resin volumes of 100 mM HEPES ...
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bioRxiv - Biochemistry 2023Quote: ... The cleared cell extract was then applied to high salt buffer equilibrated HisTrap™ HP (Cytiva Europe GmbH, Freiburg, Germany) and Strep-Tactin®XT 4Flow® high capacity (IBA Lifesciences, Göttingen, Germany) columns via a ӒKTA pure™ chromatography system (Cytiva Europe GmbH ...
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bioRxiv - Molecular Biology 2024Quote: ... and Twin-Strep tagged proteins of interest were purified from crude extracts (soluble fraction) using the Strep-Tactin®XT Superflow® Kit (IBA Lifesciences, Germany). Purification was performed according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Streptactin XT 4F High-Capacity resin was from IBA Life Sciences. [γ-32P]ATP (6,000 Ci/mmol ...
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bioRxiv - Biophysics 2023Quote: ... prior to binding to Streptactin Superflow high-capacity resin (IBA Lifesciences) equilibrated in wash buffer (25 mM HEPES ...
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bioRxiv - Cell Biology 2023Quote: ... The Strep-Tactin Superflow high-capacity resin (IBA lifesciences, 2-1208-002) was used to purify the protein ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
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bioRxiv - Microbiology 2022Quote: ... and subsequently purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences) following the manufacturer’s instructions.
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A pectin-binding peptide with a structural and signaling role in the assembly of the plant cell wallbioRxiv - Plant Biology 2023Quote: ... Ni2+ (HisTrap excel, Cytiva) and StrepII (Strep-Tactin Superflow high capacity, IBA Lifesciences) columns were equilibrated in 25mM KPi pH 7.8 ...
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bioRxiv - Immunology 2021Quote: ... Each culture media was collected and recombinant protein was purified from the supernatant using StrepTactin 4Flow® 5 mL cartridge (Iba Lifesciences) and buffers recommended by the manufacturer ...
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bioRxiv - Molecular Biology 2022Quote: ... or Strep-Tactin®XT 4Flow high capacity resin (IBA life sciences, 2-5030-002). Bound proteins were eluted with 0.2 mg/mL 1xFLAG (DYKDDDK) ...
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bioRxiv - Plant Biology 2023Quote: ... The protein was purified with a Strep-Tactin XT 4Flow high-capacity column (IBA lifesciences) and stored in 10% glycerol in buffer W at -80°C ...
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bioRxiv - Microbiology 2020Quote: ... and purified from supernatant with a Gravity Flow Strep-Tactin Superflow high capacity column (IBA Lifesciences). Purified CD81-LEL was polished by size exclusion chromatography (SEC ...
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bioRxiv - Biophysics 2022Quote: ... before being loaded into a 6 mL Strep-Tactin Superflow high capacity resin (IBA Lifesciences, USA). The resin was washed using 6 column volumes of Buffer W1 (20 mM HEPES pH 7.5 ...
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bioRxiv - Physiology 2022Quote: ... the thawed medium was loaded onto Strep-Tactin® Superflow® high capacity cartridge (IBA Lifesciences GmbH) pre-equilibrated in the binding buffer (100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... Human A3B-CTD and A3B-full were separately cloned into pASK-IBA5C plasmid vector (IBA LifeSciences) using Gibson cloning kit (ThermoFisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... High purity monomeric sE2 was generated by sequential affinity purification using StrepTactin-XT columns (IBA Lifesciences, Göttingen, Germany) and size-exclusion chromatography using the HiPrep™ 16/60 Sephacryl® S-200 HR gel filtration column (Cytiva ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting supernatant containing soluble proteins was loaded in Strep-Tactin XT 4Flow High-Capacity column (IBA lifesciences) previously saturated with N-TS-MtNCC1-78·Cu+ ...
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bioRxiv - Biochemistry 2024Quote: ... Pooled fractions containing AtRPAP3 were loaded onto a StrepTactin®XT 4Flow® high-capacity column (IBA Lifesciences) equilibrated in 25 mM HEPES pH 8 ...
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bioRxiv - Immunology 2021Quote: ... at which point the supernatant was applied to pre-equilibrated Strep-Tactin XT Superflow high-capacity resin (IBA Lifesciences) and allowed to enter the column by gravity flow ...
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bioRxiv - Cancer Biology 2019Quote: ... pooled and loaded onto a gravity column containing Strep-Tactin Superflow high capacity resin (#2-1208-025, Iba Lifesciences). Strep-tagged proteins were eluted using 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... the Ldh-EtfAB complex was purified by affinity chromatography on Strep-Tactin high-capacity material (IBA Lifesciences GmbH, Göttingen, Germany) using the elution buffer E (buffer W+ 5 mM desthiobiotin) ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was captured onto 2 mL of Strep-Tactin Superflow High-Capacity resin (IBA Lifesciences, cat. no. 2-1208-002) equilibrated with HEPES-Ca buffer (20 mM HEPES pH 7.4 ...