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Citations for IBA Life Sciences :
1 - 50 of 134 citations for Recombinant Human Catenin cadherin associated protein Beta 1 88kDa His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Twin Strep- and His6-tagged proteins were captured on Streptactin XT (IBA LifeSciences) or Talon (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... StrepII-tagged proteins were purified using Strep-Tactin MacroPrep (IBA Lifesciences, Göttingen, Germany) following the manufacturer’s instructions.
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bioRxiv - Evolutionary Biology 2024Quote: ... Strep-tagged proteins were captured on Strep-Tactin XT Superflow High Capacity (IBA LifeSciences) affinity resin ...
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bioRxiv - Microbiology 2021Quote: ... Strep-tagged proteins were purified using affinity chromatography and Strep-Tactin® Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... The strep tagged protein was purified using affinity chromatography and Strep-Tactin Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... 2xStrep tagged S-protein was stained using StrepMAB-Classic DY-488 conjugate (IBA Lifesciences, 2-1563-050) and FLAG tagged S-protein was stained using anti-FLAG (clone M2 ...
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bioRxiv - Immunology 2024Quote: ... and recombinant proteins were purified using Strep-Tactin XT resin (IBA Lifesciences, Cat. #2-5010), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore ...
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bioRxiv - Molecular Biology 2021Quote: Strep tagged proteins were then purified by incubating whole cell lysates with MagStrep “type3” XT beads (IBA Life Sciences) for five-six hours at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... the tagged NStr-M35_FL or NStr-M35_S protein were purified by StrepTactin Superflow high capacity (IBA Lifesciences, Göttingen, Germany) chromatography with a 1 mL self-made column (Mobicol ...
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bioRxiv - Immunology 2023Quote: ... 200 µg of stabilized histidine tagged S protein were labelled using Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and coated with 1 mg of magnetic beads (Dynabeads His-Tag ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
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bioRxiv - Biochemistry 2024Quote: ... Affinity purification of Strep3-tagged proteins was performed using a Strep-Tactin® Superflow® high capacity FPLC column (IBA Lifesciences).
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bioRxiv - Biochemistry 2019Quote: Fluorescently tagged Arc and StrepII tagged Arc were coexpressed in HEK293 cells and purified using Strep-Tactin Sepharose (IBA Lifesciences). Formation of complexes between the indicated Arc constructs was analyzed by immunoblotting following SDS electrophoresis ...
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bioRxiv - Molecular Biology 2024Quote: ... and Twin-Strep tagged proteins of interest were purified from crude extracts (soluble fraction) using the Strep-Tactin®XT Superflow® Kit (IBA Lifesciences, Germany). Purification was performed according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
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bioRxiv - Immunology 2021Quote: ... Each culture media was collected and recombinant protein was purified from the supernatant using StrepTactin 4Flow® 5 mL cartridge (Iba Lifesciences) and buffers recommended by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... or strep-tagged spike immobilized on Strep-Tactin XT magnetic beads (IBA Lifesciences). To select for non-RBD binders ...
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bioRxiv - Biochemistry 2021Quote: ... Twin-Strep-tagged mTOR was captured onto a Strep-Tactin XT (IBA Lifesciences) coated surface prepared according to the supplied instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... twin-strep tagged MKM containing lysates were incubated with Streptactin XT magnetic bead slurry (IBA Lifesciences) and purified according to manufacturer’s recommendation ...
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bioRxiv - Genetics 2021Quote: ... Strep-tagged Orf8 complexes were eluted from beads in Buffer BXT (IBA Lifesciences; Cat. # 2-1042-025) shaking at 1100 rpm for 30 min.
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bioRxiv - Genetics 2021Quote: ... Strep-tagged Orf8 complexes were eluted from beads in Buffer BXT (IBA Lifesciences; Cat. # 2-1042-025) shaking at 1100 rpm for 30 min.
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bioRxiv - Immunology 2023Quote: ... TwinStrep tagged 8.9F was purified via gravity column before being eluted by 1X BXT (IBA Life Sciences) while 6x-His tagged 8.9F and 18.5C culture supernatants were rolled overnight with Ni-NTA beads at 4°C before using gravity columns to capture the resin ...
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bioRxiv - Biophysics 2023Quote: ... the tagged d1_d2 was purified from the supernatant by affinity chromatography using Strep-Tactin resin (IBA Lifesciences, Göttingen, Germany).
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bioRxiv - Molecular Biology 2021Quote: ... 2xStrep tagged SARS-CoV-2 ORFs were immunoprecipitated using 30 μl of Megastrep type 3 XT beads (IBA LifeSciences, Germany), and flag-tagged SARS-CoV-2 ORFs were immunoprecipitated using Anti-DYKDDDDK Magnetic Agarose (Pierce ...
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bioRxiv - Immunology 2020Quote: ... was used to produce twin-strep-tagged fusion chemokines which were detected with an HRP-conjugated anti-Streptag mAb (IBA LifeSciences). Plates were developed with TMB One Component (Surmodics ...
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bioRxiv - Immunology 2021Quote: ... 3C-digested proteins were loaded into StrepTactin XT 4Flow® 1 mL gravity flow columns (Iba Lifesciences) and collected in the flow-through for quantification ...
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bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged: The lysate was passed through chromatography columns containing 1.5 ml bed volume of Strep-Tactin® Sepharose® resin (IBA Lifesciences, Germany). Resin was washed with ∼20 resin volumes of 100 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... The column was washed until baseline and the protein was eluted with PBS containing 1 mM DTT and 2.5 mM desthiobiotin (IBA Lifesciences). Fractions were pooled based on the chromatogram and SDS-PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20 μL Strep-Tactin Macroprep beads (IBA lifesciences). Beads were washed three times with lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20-30 μl Strep-Tactin Macroprep beads (IBA lifesciences), FLAG or Ni-NTA beads (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleaved-off alkaline phosphatase and TEV protease were removed by re-purifying EXT1-EXT2 using a second HisTrap HP purification step and by passing protein sample through 1 mL Strep-Tactin XT resin (IBA Lifesciences). Buffer was exchanged by several rounds of concentration and dilution with buffer B (20mM HEPES pH 7.2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The solution was then centrifuged 3 times at 20,000 × g at 4 °C for 10 min periods and the supernatant was used for purification using fast protein liquid chromatography (FPLC) and 1 mL Strep-Tactin Superflow high capacity column (IBA Lifesciences, Germany). After equilibration with 5 column volume (CV ...
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bioRxiv - Immunology 2021Quote: ... Expressed protein was incubated with BioLock (IBA Lifesciences) and then isolated by Strep-tag affinity chromatography on StrepTrap HP columns (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were eluted with 1X Buffer BX (IBA LifeSciences). Purified proteins were visualized on a BOLT 4-12% Bis-Tris Plus gel (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were eluted with 1X Buffer BX (IBA LifeSciences) which contains 10 mM D-biotin ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were purified using Strep-Tactin Superflow resin (IBA Lifesciences) employing four washing steps ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble protein was loaded on Strep-TactinXT beads (IBA Lifesciences) and eluted with 50 mM Biotin (Applichem ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted protein was applied to Streptactin-XT resin (IBA lifesciences) equilibrated with equilibration buffer (100 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... Human A3B-CTD and A3B-full were separately cloned into pASK-IBA5C plasmid vector (IBA LifeSciences) using Gibson cloning kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified on Streptactin XT superflow beads (IBA Lifesciences) and eluted with 50 mM NaH2PO4.2H2O pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was incubated with Strep-Tactin Sepharose (IBA Lifesciences) beads ...
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bioRxiv - Biophysics 2023Quote: ... the protein was purified using Streptactin affinity resin (IBA Life Sciences). Resin bound protein was washed as before and eluted using wash buffer ...
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bioRxiv - Immunology 2023Quote: ... The proteins were eluted using 1X BXT buffer (IBA Life Sciences) and buffer exchanged to 1X TBS using 100 kDa MWCO concentrators for trimers and 10 kDa MWCO concentrators for monomers (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified by using StrepTactin resin column (IBA Life sciences) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Purified proteins were then eluted by desthiobiotin using Buffer E (IBA Lifesciences) and subsequently prepared for immunoblotting as described above ...
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bioRxiv - Neuroscience 2023Quote: ... Final pure protein was eluted with Buffer BXT +10% glycerol (IBA Lifesciences) or BRB80 (80 mM PIPES-KOH ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble protein fraction was loaded on Strep-TactinXT beads (IBA Lifesciences) and eluted with 50 mM Biotin (Applichem ...
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bioRxiv - Cell Biology 2024Quote: ... Strep protein was immunoprecipitated with 20 uL MagStrep “type3” XT beads (IBA Lifesciences) pre-washed with IP wash buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... the protein was detected using the strep-tactin HRP conjugate (IBA Lifesciences, Göttingen, Germany) as specified by the manufacturer ...
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bioRxiv - Plant Biology 2023Quote: ... the eluted protein was next loaded onto a StrepTactin Sepharose column (IBA Life Sciences). The column was washed with buffer W (100 mM Tris pH 8.0 ...