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Citations for IBA Life Sciences :
51 - 74 of 74 citations for Rat Corticosteroid 11 Beta Dehydrogenase Isozyme 2 HSD11B2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Purified E1E2 + AR4A-StrepII complexes (1.0 μg ml™1 in TBS with 0.1% DDM) were coated for 2 h at room temperature on 96-well Strep-Tactin XT coated microplates (IBA LifeSciences). Plates were washed twice with TBS/0.05% Tween20 before incubating with serially diluted mAbs and CD81-LEL-hFc (R&D systems ...
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bioRxiv - Microbiology 2023Quote: ... Concentrated proteins were resuspended in buffer W at pH 8 and incubated with BioLock (#2-0205-050, Iba Life sciences) for 15 min at RT to neutralize biotin ...
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bioRxiv - Immunology 2024Quote: ... stabilized histidine-tagged SARS-CoV-2 S protein (Wuhan, XBB.1.5, BA.2.86) was labelled with Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and conjugated to magnetic beads according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble membrane fraction was diluted 2-fold with buffer B and applied to a column packed with Strep-Tactin beads (IBA Lifesciences) by gravity flow (6–10 s/drop ...
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bioRxiv - Microbiology 2020Quote: Purified StrepII-tagged sE2 monomer (1.0 µg/mL in TBS) was coated for 2 hour at room temperature on 96-well Strep-TactinXT coated microplates (IBA LifeSciences). Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems ...
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bioRxiv - Plant Biology 2020Quote: ... They were next incubated in primary antibody solution for 2 hours at room temperature (1:1000 anti-Strep, IBA Life Sciences, 1:10,000 anti-FLAG M2 monoclonal antibody ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gravity flow columns (2 mL) were prepared with 400 μL 50% Strep-Tactin® XT Superflow High Capacity suspension (IBA Lifesciences). Protein purification was performed according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... Cellular debris was pelleted by ultracentrifugation before placing lysate over a Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Insoluble materials were discarded and clear lysates were added to Strep-Tactin sepharose (400 μL 50% slurry; IBA Lifesciences #2-1201-010) loaded columns (Bio-Rad Laboratories #732-6008 ...
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bioRxiv - Molecular Biology 2023Quote: ... we added an additional purification step and incubated the Flag-eluted samples with Strep-TactinXT 4Flow beads (IBA lifesciences, 2-5030-010) for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was washed five times with IP buffer and eluted with Strep-Tactin elution buffer (IBA Life Sciences, 2-1000-025). Eluted samples were analyzed by western blot.
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bioRxiv - Microbiology 2024Quote: ... 0.5-1 mg of lysate was incubated overnight with 30 ul of Strep-Tactin Sepharose resin (IBA Life Science, 2-1201-002), rotating at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The Streptag pulldown was completed in the same manner using 100 μL of MagStrep "type3" XT bead suspension (IBA Lifesciences, 2-4090-002), except elution was achieved by boiling with sample buffer at 95 °C for 2 minutes.
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bioRxiv - Immunology 2019Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences # 2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001 ...
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bioRxiv - Microbiology 2022Quote: ... incubated with anti-StrepMAB-Classic-HRP antibody that recognizes the strep tag at the C-terminus of the SFV Env ectodomain (7.5 μg/ml, IBA Lifesciences #2-1509-001) for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... in TSM binding buffer at 4°C for 1 h before StrepMAB-Classic Oyster 645 conjugate (0.5 μg/mL, IBA Lifesciences 2-1555-050) was added ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were incubated for 1 h at room temperature in blocking solution with mouse StrepMAB antibody (IBA Lifesciences, 1:750, 2-1507-001) for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8 ...
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bioRxiv - Immunology 2023Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences #2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences #2-1507-001 ...
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bioRxiv - Biochemistry 2024Quote: ... 5 min) and incubated on ice for 30 min with 100 nM scFv and StrepTactin-XT DY647 conjugate (IBA Lifesciences, 2-1568-050) at a 1:200 dilution in 1% BSA in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... with half immunoprecipitated for FLAG-ATF6 with 15 μL G1 anti-DYKDDDK resin and the other half affinity purified for nsp3.1-ST using 15 μL Strep-Tactin XT Superflow High Capacity resin (IBA Life Sciences, 2-4030-002) and rocked at 4°C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... The Twin-Strep-tag® capture kit was from Iba Lifesciences (IBA GmbH, Germany). Peptides were purchased from Peptide 2.0 Inc (Chantilly ...
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bioRxiv - Biophysics 2021Quote: ... The TS-SUMO-SaCas9-sgRNA or TS-SUMO-SaCas9 cell lysate was applied to a 5 mL Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer containing 200 mM NaCl ...
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bioRxiv - Microbiology 2020Quote: ... Primary CD4+ T cells were harvested by positive selection using a FABian automated enrichment system and CD4 isolation kit (IBA Lifesciences). Isolated T cells were suspended in complete RPMI-1640 medium supplemented with 5 mM HEPES ...
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bioRxiv - Molecular Biology 2024Quote: ... and Twin-Strep tagged proteins of interest were purified from crude extracts (soluble fraction) using the Strep-Tactin®XT Superflow® Kit (IBA Lifesciences, Germany). Purification was performed according to the manufacturer’s instructions ...