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Citations for IBA Life Sciences :
51 - 100 of 132 citations for Mouse Vacuole Membrane Protein 1 VMP1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The bound proteins were eluted with the binding buffer supplemented with 10 mM desthiobiotin (IBA Lifesciences). The samples were analyzed by SDS-PAGE and detected by immunoblotting using antibodies to the strep tag (1:3,000 ...
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bioRxiv - Microbiology 2022Quote: ... 2xStrep tagged S-protein was stained using StrepMAB-Classic DY-488 conjugate (IBA Lifesciences, 2-1563-050) and FLAG tagged S-protein was stained using anti-FLAG (clone M2 ...
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bioRxiv - Biochemistry 2022Quote: ... The S protein was eluted by Buffer A containing 0.02% DDM and 5 mM desthiobiotin (IBA Lifesciences). The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound proteins were eluted with the binding buffer supplemented with 10 mM desthiobiotin (IBA Lifesciences). The samples were analyzed by SDS-PAGE and detected by Coomassie staining.
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bioRxiv - Molecular Biology 2024Quote: ... jejuni 81-116) and Abu0127 proteins were purified according to the Strep-Tactin manufacturer’s protocol (IBA Lifesciences). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... StrepMAB-Classic (IBA LifeSciences, WB 1:2000, IF 1:500), StrepMAB-Classic DY-549 (IBA LifeSciences ...
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bioRxiv - Biochemistry 2020Quote: ... Viral proteins were affinity purified from 4mg lysates by tumbling with 40µl Strep-Tactin Sepharose (IBA Life Sciences) for 90min at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Elution fractions containing the Tmod proteins were then loaded onto Streptactin XT resin (IBA Life Sciences, Göttingen, Germany) and washed with 20 column volumes of strep buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting supernatant containing soluble proteins was loaded in Strep-Tactin XT 4Flow High-Capacity column (IBA lifesciences) previously saturated with N-TS-MtNCC1-78·Cu+ ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were further purified by loading His60 eluate onto Strep-TactinXT affinity chromatography gravity columns (IBA Lifesciences, Germany). Final pure protein was eluted with Buffer BXT +10% glycerol (IBA Lifesciences ...
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bioRxiv - Biochemistry 2023Quote: ... The bound protein was eluted in 3 CV buffer W supplemented with 2.5 mM desthiobiotin (IBA Life Sciences). This material was then applied to a MonoQ 5/50 GL (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... Spike proteins in culture medium were purified with gravity flow columns using Strep-Tactin XT (IBA Life Sciences), concentrated to 0.5 mg/ml with ultrafiltration tubes (Millipore ...
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bioRxiv - Systems Biology 2023Quote: ... 1 mM DTT) and 50 μl 1× buffer BXT (IBA Lifesciences) and dispensed into a single 96-well KingFisher microtitre plate ...
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bioRxiv - Cell Biology 2019Quote: ... 5’-end-labelled RNA fragments from mouse β-actin mRNA (accession number: NM_007393.5) and mouse β2B-tubulin-mRNA (accession number: NM_023716.2) were purchased from IBA Lifesciences (Germany).
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bioRxiv - Molecular Biology 2021Quote: Strep tagged proteins were then purified by incubating whole cell lysates with MagStrep “type3” XT beads (IBA Life Sciences) for five-six hours at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The Twin-Strep tag and Strep-Tactin XT protein fluorescently conjugated with DY-649 were purchased from IBA Lifesciences.
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bioRxiv - Biophysics 2022Quote: ... The proteins were purified via a C-terminal STREP II tag using Strep-tactin resin (IBA LifeSciences, Göttingen, DE). The solubilizing detergents were replaced by 0.06% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... the tagged NStr-M35_FL or NStr-M35_S protein were purified by StrepTactin Superflow high capacity (IBA Lifesciences, Göttingen, Germany) chromatography with a 1 mL self-made column (Mobicol ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein was purified from the supernatant by batch binding to Strep-Tactin XT Superflow resin (IBA Lifesciences). The resin was washed initially with Buffer A containing 0.05 % (w/v ...
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bioRxiv - Microbiology 2019Quote: ... Soluble proteins were loaded on self-prepared columns packed with Strep-Tactin XT superflow or Strep-Tactin Sepharose (IBA lifesciences) and purified under gravity flow ...
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bioRxiv - Biochemistry 2021Quote: ... Protein detection on the blots was performed with an antibody against the Strep-tag II (StrepMAB-Classic, IBA Lifesciences, Germany).
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bioRxiv - Microbiology 2021Quote: ... The protein was expressed and lysed as described above and purified according to the manufacturer’s guidelines (IBA Life Sciences, Inc.). Clarified lysates were passed over Strep-Tactin-Sepharose resin using a gravity filtration column ...
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bioRxiv - Immunology 2023Quote: ... 200 µg of stabilized histidine tagged S protein were labelled using Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and coated with 1 mg of magnetic beads (Dynabeads His-Tag ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was captured onto 2 mL of Strep-Tactin Superflow High-Capacity resin (IBA Lifesciences, cat. no. 2-1208-002) equilibrated with HEPES-Ca buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... Concentrated proteins were resuspended in buffer W at pH 8 and incubated with BioLock (#2-0205-050, Iba Life sciences) for 15 min at RT to neutralize biotin ...
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bioRxiv - Immunology 2024Quote: ... stabilized histidine-tagged SARS-CoV-2 S protein (Wuhan, XBB.1.5, BA.2.86) was labelled with Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and conjugated to magnetic beads according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the proteins were eluted in 1x BXT buffer (150 mM NaCl, 50 mM Tris-HCl pH 8.0, 1mM EDTA; IBA LifeSciences). We then exchanged the buffer to the protein buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Beads were then washed with buffer B and proteins were eluted using buffer B supplemented with 10 mM desthiobiotin (IBA Lifesciences). Samples were taken at each step of the purification and loaded on SDS-PAGE gels to check the efficiency of affinity purification ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
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bioRxiv - Plant Biology 2023Quote: ... The elution fractions containing the trxA-14-3-3 or trxA-BIN2 fusion proteins were loaded onto a 5 ml Strep-Tactin Superflow High Capacity column (IBA Lifesciences), washed with buffer A and eluted in buffer A supplemented with 2.5 mM desthiobiotin ...
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bioRxiv - Microbiology 2023Quote: Secreted twin StrepTagged gp16-V1V2 proteins in the harvested and filtered supernatant were supplemented with BioLock biotin blocking solution (IBA Lifesciences) at 5 µl/ml to mask the biotin present in the supernatant ...
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bioRxiv - Microbiology 2023Quote: ... supernatant from cells incubated with 4 µM CdCl2 for 5 days was collected and soluble protein was purified by affinity chromatography using a Strep-Tactin XT 4Flow column (IBA Lifesciences) followed by size exclusion chromatography using a HiLoad 26/600 superdex 200 pg column (Cytiva ...
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bioRxiv - Biochemistry 2024Quote: ... Affinity purification of Strep3-tagged proteins was performed using a Strep-Tactin® Superflow® high capacity FPLC column (IBA Lifesciences).
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bioRxiv - Immunology 2021Quote: ... Each culture media was collected and recombinant protein was purified from the supernatant using StrepTactin 4Flow® 5 mL cartridge (Iba Lifesciences) and buffers recommended by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was washed five times with IP buffer and eluted with Strep-Tactin elution buffer (IBA Life Sciences, 2-1000-025). Eluted samples were analyzed by western blot.
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... The bound proteins were removed by boiling in sample buffer and subjected to immunoblotting analysis using mouse monoclonal anti-6xHis (Yao-Hong Biotech. Inc., TW) and Strep-tagII (IBA Lifesciences, Germany) primary antibodies and the goat anti-mouse IgG-HRP-conjugated secondary antibody (Thermo Scientific ...
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bioRxiv - Genetics 2023Quote: ... 1 ml StrepTactin (IBA Lifesciences, Göttingen, Germany) pre-equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Strep-Tactin-DY649 (IBA LifeSciences, IF 1:2000), mouse anti-CIMPR (2G11 ...
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bioRxiv - Cell Biology 2024Quote: ... The Twin-Strep-tag® capture kit was from Iba Lifesciences (IBA GmbH, Germany). Peptides were purchased from Peptide 2.0 Inc (Chantilly ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) containing 2.5 mM desthiobiotin (IBA Lifesciences). Eluted protein was concentrated and run on a Superose 6 10/300 GL size exclusion column (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mL of BioLock biotin blocking solution (IBA Lifesciences) were added to 1.5 L conditioned medium ...
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bioRxiv - Biochemistry 2022Quote: ... and StrepMAB classic DY649-conjugate (1:250, IBA Lifesciences) for the unstained samples ...
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bioRxiv - Biochemistry 2022Quote: ... and StrepMAB classic DY649-conjugate (1:150, IBA Lifesciences) for the unstained samples ...
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bioRxiv - Cell Biology 2022Quote: ... StrepMAB-Classic DY-549 (IBA LifeSciences, FC 1:500), Strep-Tactin-DY649 (IBA LifeSciences ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with 1 ml Strep-Tactin resin (IBA Lifesciences) for 2 hours at 4°C with rotation ...
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bioRxiv - Neuroscience 2021Quote: ... Twin-Strep-tag® (IBA Lifesciences; 2-1517-001; 1:1000); and Streptavidin 660 Conjugate (ThermoFisher Scientific ...
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bioRxiv - Systems Biology 2020Quote: ... 1 mM DTT) and 50 μl 1x Buffer BXT (IBA Lifesciences) dispensed into a single 96-well KF microtiter plate ...
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bioRxiv - Genetics 2021Quote: ... streptactin DY-488 (IBA Lifesciences; Cat. # 2-1562-050; 1:500) was added to secondary antibody solution ...
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bioRxiv - Biochemistry 2023Quote: 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h gently stirring at 4°C ...