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Citations for IBA Life Sciences :
1 - 50 of 137 citations for Methyl CpG binding protein 2 MECP2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... The bound proteins were eluted with the binding buffer supplemented with 10 mM desthiobiotin (IBA Lifesciences). The samples were analyzed by SDS-PAGE and detected by immunoblotting using antibodies to the strep tag (1:3,000 ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound proteins were eluted with the binding buffer supplemented with 10 mM desthiobiotin (IBA Lifesciences). The samples were analyzed by SDS-PAGE and detected by Coomassie staining.
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bioRxiv - Biochemistry 2023Quote: ... and the protein was purified from the supernatant by batch binding to Strep-Tactin XT Superflow resin (IBA Lifesciences). The resin was washed initially with Buffer A containing 0.05 % (w/v ...
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bioRxiv - Immunology 2022Quote: ... in TSM binding buffer at 4°C for 1 h before StrepMAB-Classic Oyster 645 conjugate (0.5 μg/mL, IBA Lifesciences 2-1555-050) was added ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was captured onto 2 mL of Strep-Tactin Superflow High-Capacity resin (IBA Lifesciences, cat. no. 2-1208-002) equilibrated with HEPES-Ca buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2023Quote: ... prior to binding to Streptactin Superflow high-capacity resin (IBA Lifesciences) equilibrated in wash buffer (25 mM HEPES ...
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bioRxiv - Molecular Biology 2022Quote: ... SARS-COV-2 NTD proteins were purified with Sepharose Strep-Tactin beads (IBA Life Sciences) as previously described [75].
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bioRxiv - Immunology 2024Quote: ... and recombinant proteins were purified using Strep-Tactin XT resin (IBA Lifesciences, Cat. #2-5010), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore ...
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bioRxiv - Microbiology 2022Quote: ... 2xStrep tagged S-protein was stained using StrepMAB-Classic DY-488 conjugate (IBA Lifesciences, 2-1563-050) and FLAG tagged S-protein was stained using anti-FLAG (clone M2 ...
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bioRxiv - Biochemistry 2019Quote: Fluorescently tagged Arc and StrepII tagged Arc were coexpressed in HEK293 cells and purified using Strep-Tactin Sepharose (IBA Lifesciences). Formation of complexes between the indicated Arc constructs was analyzed by immunoblotting following SDS electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... Concentrated proteins were resuspended in buffer W at pH 8 and incubated with BioLock (#2-0205-050, Iba Life sciences) for 15 min at RT to neutralize biotin ...
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bioRxiv - Immunology 2024Quote: ... stabilized histidine-tagged SARS-CoV-2 S protein (Wuhan, XBB.1.5, BA.2.86) was labelled with Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and conjugated to magnetic beads according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was washed five times with IP buffer and eluted with Strep-Tactin elution buffer (IBA Life Sciences, 2-1000-025). Eluted samples were analyzed by western blot.
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bioRxiv - Immunology 2021Quote: ... and further purified using a 2 mL StrepTactin sepharose column (IBA Lifesciences Cat# 2-1201-002) and a Strep-Tactin Purification Buffer Set (IBA Lifesciences Cat#2-1002-001) ...
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bioRxiv - Biochemistry 2023Quote: ... or 2 mL of Strep-Tactin® Sepharose® resin (IBA Lifesciences GmbH, #2-1201-002) for strep-II tagged proteins ...
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bioRxiv - Immunology 2021Quote: ... Expressed protein was incubated with BioLock (IBA Lifesciences) and then isolated by Strep-tag affinity chromatography on StrepTrap HP columns (GE Healthcare) ...
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bioRxiv - Microbiology 2021Quote: ... Samples stained with trimeric SARS-CoV-2 RBD were incubated with primary antibody strepmabHRP (2-1509-001, IBA lifesciences) with specificity towards the TwinStrep-tag at the C-terminus of the trimeric protein for 120 hours in PBSGT with 0.1% saponin ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were eluted with 1X Buffer BX (IBA LifeSciences). Purified proteins were visualized on a BOLT 4-12% Bis-Tris Plus gel (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were eluted with 1X Buffer BX (IBA LifeSciences) which contains 10 mM D-biotin ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 mM desthiobiotin (IBA lifesciences, 2-1000-002). The eluted fractions containing highest concentration of the expressed protein were pooled and dialyzed against the storage buffer containing 10mM Tris/HCl ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were purified using Strep-Tactin Superflow resin (IBA Lifesciences) employing four washing steps ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble protein was loaded on Strep-TactinXT beads (IBA Lifesciences) and eluted with 50 mM Biotin (Applichem ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted protein was applied to Streptactin-XT resin (IBA lifesciences) equilibrated with equilibration buffer (100 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... Human A3B-CTD and A3B-full were separately cloned into pASK-IBA5C plasmid vector (IBA LifeSciences) using Gibson cloning kit (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified on Streptactin XT superflow beads (IBA Lifesciences) and eluted with 50 mM NaH2PO4.2H2O pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was incubated with Strep-Tactin Sepharose (IBA Lifesciences) beads ...
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bioRxiv - Biophysics 2023Quote: ... the protein was purified using Streptactin affinity resin (IBA Life Sciences). Resin bound protein was washed as before and eluted using wash buffer ...
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bioRxiv - Immunology 2023Quote: ... The proteins were eluted using 1X BXT buffer (IBA Life Sciences) and buffer exchanged to 1X TBS using 100 kDa MWCO concentrators for trimers and 10 kDa MWCO concentrators for monomers (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified by using StrepTactin resin column (IBA Life sciences) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Purified proteins were then eluted by desthiobiotin using Buffer E (IBA Lifesciences) and subsequently prepared for immunoblotting as described above ...
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bioRxiv - Neuroscience 2023Quote: ... Final pure protein was eluted with Buffer BXT +10% glycerol (IBA Lifesciences) or BRB80 (80 mM PIPES-KOH ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble protein fraction was loaded on Strep-TactinXT beads (IBA Lifesciences) and eluted with 50 mM Biotin (Applichem ...
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bioRxiv - Neuroscience 2021Quote: ... Twin-Strep-tag® (IBA Lifesciences; 2-1517-001; 1:1000); and Streptavidin 660 Conjugate (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences), and SARS-CoV-2 spike-StreptTactin APC (StrepTactin APC from IBA Lifesciences).
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences), SARS-CoV-2 ΔRBM RBD-APC (streptavidin-APC from BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences), SARS-CoV-2 ΔRBM RBD-PE/Cy5.5 (streptavidin-PE/Cy5.5 from BioLegend) ...
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bioRxiv - Immunology 2019Quote: ... or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001) and anti-mouse IgG1 antibody Alexa Fluor 647 (Thermo Fisher Scientific # A-21240) ...
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bioRxiv - Genetics 2021Quote: ... streptactin DY-488 (IBA Lifesciences; Cat. # 2-1562-050; 1:500) was added to secondary antibody solution ...
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bioRxiv - Microbiology 2022Quote: ... StrepMAB-Classic (1:500, mouse monoclonal, IBA lifesciences, 2-1507-001), anti-SARS-CoV-2 Spike (1:100 ...
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bioRxiv - Immunology 2023Quote: ... or anti-Strep-tag II antibody (IBA Lifesciences #2-1507-001) and anti-mouse IgG1 antibody Alexa Fluor 647 (Thermo Fisher Scientific #A-21240) ...
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bioRxiv - Biochemistry 2024Quote: ... and purified on StrepTactin-XT resin (IBA Lifesciences, 2-5010-002) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Twin Strep- and His6-tagged proteins were captured on Streptactin XT (IBA LifeSciences) or Talon (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... StrepII-tagged proteins were purified using Strep-Tactin MacroPrep (IBA Lifesciences, Göttingen, Germany) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Strep protein was immunoprecipitated with 20 uL MagStrep “type3” XT beads (IBA Lifesciences) pre-washed with IP wash buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 spike-StreptTactin APC (StrepTactin APC from IBA Lifesciences).
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bioRxiv - Immunology 2020Quote: ... and Strep-Tactin Purification Buffer Set (IBA Lifesciences Cat # 2-1002-001) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h gently stirring at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and S1-StrepTactin XT DY-649 (IBA life sciences, 2-1568-050) and S1-StrepTactin XT DY-488 (IBA life sciences ...
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bioRxiv - Immunology 2023Quote: ... and S1-StrepTactin XT DY-488 (IBA life sciences, 2-1562- 050). Live CD3/CD8-CD14-CD19+IgM-IgD-IgG+S1+S1+ cells were sorted using a BD FACS Melody into individual wells containing RNase OUT (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... The Strep-Tactin Superflow high-capacity resin (IBA lifesciences, 2-1208-002) was used to purify the protein ...