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Citations for IBA Life Sciences :
51 - 95 of 95 citations for Human Single stranded DNA binding protein 4 SSBP4 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... jejuni 81-116) and Abu0127 proteins were purified according to the Strep-Tactin manufacturer’s protocol (IBA Lifesciences). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... lysates were clarified by centrifugation (17,000 x rpm for 40 min at 4 °C) and incubated with Strep-Tactin Sepharose (IBA Lifesciences) at 4 °C overnight ...
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bioRxiv - Biochemistry 2020Quote: ... Viral proteins were affinity purified from 4mg lysates by tumbling with 40µl Strep-Tactin Sepharose (IBA Life Sciences) for 90min at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Elution fractions containing the Tmod proteins were then loaded onto Streptactin XT resin (IBA Life Sciences, Göttingen, Germany) and washed with 20 column volumes of strep buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting supernatant containing soluble proteins was loaded in Strep-Tactin XT 4Flow High-Capacity column (IBA lifesciences) previously saturated with N-TS-MtNCC1-78·Cu+ ...
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bioRxiv - Neuroscience 2023Quote: ... Proteins were further purified by loading His60 eluate onto Strep-TactinXT affinity chromatography gravity columns (IBA Lifesciences, Germany). Final pure protein was eluted with Buffer BXT +10% glycerol (IBA Lifesciences ...
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bioRxiv - Biochemistry 2023Quote: ... The bound protein was eluted in 3 CV buffer W supplemented with 2.5 mM desthiobiotin (IBA Life Sciences). This material was then applied to a MonoQ 5/50 GL (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... Spike proteins in culture medium were purified with gravity flow columns using Strep-Tactin XT (IBA Life Sciences), concentrated to 0.5 mg/ml with ultrafiltration tubes (Millipore ...
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bioRxiv - Biochemistry 2021Quote: Labelled and unlabelled DNA oligos to make lacCONS+2 promoter constructs were purchased from IBA Lifesciences (Germany) (Fig ...
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bioRxiv - Biophysics 2019Quote: ... The lysate was centrifuged (30 min, 60,000xg, 4°C) and the supernatant was incubated with strep-tactin resin (Strep-Tactin® resin, Iba Lifesciences) for 30min at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... which were incubated for 30 min at 4°C with 50 ul of ferrimagnetic StrepTactin beads (MagStrep Type3 XT beads, IBA LifeSciences) preincubated in 0.1M Tris pH8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was centrifuged at 30,000 x g for 40 min at 4 °C in a JA 20 rotor and the supernatant was mixed with Strep-Tactin resin (IBA Lifesciences) previously equilibrated with buffer A ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: nProtein A Sepharose 4 Fast Flow resin (Cytiva) was used for affinity purification of adalimumab whilst Strep-Tactin XT 4Flow resin (IBA Lifesciences) was used for Strep-II tagged-tagged proteins ...
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bioRxiv - Biophysics 2023Quote: ... Supernatants were incubated with end-over-end rotation for 1 hour at 4°C with Strep-Tactin Superflow high-capacity resin (IBA Lifesciences) equilibrated with gel-filtration buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation at 50,000 g for 30 minutes at 4°C and applied to pre-equilibrated StrepTactin resin (IBA Life Sciences). The soluble extract was incubated with the resin for one hour at 4°C with constant rotation ...
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bioRxiv - Molecular Biology 2021Quote: Strep tagged proteins were then purified by incubating whole cell lysates with MagStrep “type3” XT beads (IBA Life Sciences) for five-six hours at 4 °C ...
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bioRxiv - Cell Biology 2022Quote: ... The Twin-Strep tag and Strep-Tactin XT protein fluorescently conjugated with DY-649 were purchased from IBA Lifesciences.
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bioRxiv - Biophysics 2022Quote: ... The proteins were purified via a C-terminal STREP II tag using Strep-tactin resin (IBA LifeSciences, Göttingen, DE). The solubilizing detergents were replaced by 0.06% (w/v ...
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bioRxiv - Molecular Biology 2023Quote: ... the tagged NStr-M35_FL or NStr-M35_S protein were purified by StrepTactin Superflow high capacity (IBA Lifesciences, Göttingen, Germany) chromatography with a 1 mL self-made column (Mobicol ...
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bioRxiv - Biochemistry 2023Quote: ... Then the lysates were centrifuged at 15,000 x g for 20 min at 4°C and the supernatant was passed over a column packed with Strep-tactin XT 4Flow resin (Iba Lifesciences GmbH). The column was washed with lysis buffer ...
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bioRxiv - Microbiology 2019Quote: ... Soluble proteins were loaded on self-prepared columns packed with Strep-Tactin XT superflow or Strep-Tactin Sepharose (IBA lifesciences) and purified under gravity flow ...
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bioRxiv - Biochemistry 2021Quote: ... Protein detection on the blots was performed with an antibody against the Strep-tag II (StrepMAB-Classic, IBA Lifesciences, Germany).
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bioRxiv - Microbiology 2021Quote: ... The protein was expressed and lysed as described above and purified according to the manufacturer’s guidelines (IBA Life Sciences, Inc.). Clarified lysates were passed over Strep-Tactin-Sepharose resin using a gravity filtration column ...
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bioRxiv - Molecular Biology 2022Quote: ... The column was washed until baseline and the protein was eluted with PBS containing 1 mM DTT and 2.5 mM desthiobiotin (IBA Lifesciences). Fractions were pooled based on the chromatogram and SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: ... 200 µg of stabilized histidine tagged S protein were labelled using Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and coated with 1 mg of magnetic beads (Dynabeads His-Tag ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was captured onto 2 mL of Strep-Tactin Superflow High-Capacity resin (IBA Lifesciences, cat. no. 2-1208-002) equilibrated with HEPES-Ca buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... Concentrated proteins were resuspended in buffer W at pH 8 and incubated with BioLock (#2-0205-050, Iba Life sciences) for 15 min at RT to neutralize biotin ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the proteins were eluted in 1x BXT buffer (150 mM NaCl, 50 mM Tris-HCl pH 8.0, 1mM EDTA; IBA LifeSciences). We then exchanged the buffer to the protein buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... stabilized histidine-tagged SARS-CoV-2 S protein (Wuhan, XBB.1.5, BA.2.86) was labelled with Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and conjugated to magnetic beads according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Beads were then washed with buffer B and proteins were eluted using buffer B supplemented with 10 mM desthiobiotin (IBA Lifesciences). Samples were taken at each step of the purification and loaded on SDS-PAGE gels to check the efficiency of affinity purification ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleaved-off alkaline phosphatase and TEV protease were removed by re-purifying EXT1-EXT2 using a second HisTrap HP purification step and by passing protein sample through 1 mL Strep-Tactin XT resin (IBA Lifesciences). Buffer was exchanged by several rounds of concentration and dilution with buffer B (20mM HEPES pH 7.2 ...
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bioRxiv - Microbiology 2023Quote: ... supernatant from cells incubated with 4 µM CdCl2 for 5 days was collected and soluble protein was purified by affinity chromatography using a Strep-Tactin XT 4Flow column (IBA Lifesciences) followed by size exclusion chromatography using a HiLoad 26/600 superdex 200 pg column (Cytiva ...
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bioRxiv - Plant Biology 2023Quote: ... The elution fractions containing the trxA-14-3-3 or trxA-BIN2 fusion proteins were loaded onto a 5 ml Strep-Tactin Superflow High Capacity column (IBA Lifesciences), washed with buffer A and eluted in buffer A supplemented with 2.5 mM desthiobiotin ...
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bioRxiv - Microbiology 2023Quote: Secreted twin StrepTagged gp16-V1V2 proteins in the harvested and filtered supernatant were supplemented with BioLock biotin blocking solution (IBA Lifesciences) at 5 µl/ml to mask the biotin present in the supernatant ...
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bioRxiv - Biochemistry 2024Quote: ... Affinity purification of Strep3-tagged proteins was performed using a Strep-Tactin® Superflow® high capacity FPLC column (IBA Lifesciences).
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bioRxiv - Microbiology 2024Quote: ... Cell debris was removed by centrifugation at 30,000xg and 4°C for 45 min and the resulting crude extract was applied onto a Strep-Tactin®XT 4Flow® column (IBA Lifesciences, Germany) and purified following the manufacturers’ instructions ...
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bioRxiv - Biophysics 2020Quote: The codon optimized sequence for the SARS-CoV-2 nsp7-8 region (NC_045512.2) was synthesized by Eurofins scientific SE with overhangs suitable for insertion into pASK-IBA33plus plasmid DNA (IBA Life Sciences). A golden gate assembly approach using Eco31I (BsaI ...
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bioRxiv - Synthetic Biology 2022Quote: ... The solution was then centrifuged 3 times at 20,000 × g at 4 °C for 10 min periods and the supernatant was used for purification using fast protein liquid chromatography (FPLC) and 1 mL Strep-Tactin Superflow high capacity column (IBA Lifesciences, Germany). After equilibration with 5 column volume (CV ...
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bioRxiv - Immunology 2021Quote: ... Each culture media was collected and recombinant protein was purified from the supernatant using StrepTactin 4Flow® 5 mL cartridge (Iba Lifesciences) and buffers recommended by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was washed five times with IP buffer and eluted with Strep-Tactin elution buffer (IBA Life Sciences, 2-1000-025). Eluted samples were analyzed by western blot.
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bioRxiv - Biophysics 2019Quote: ... DNA labeled at two locations for ensemble FRET experiments was prepared by large scale PCR of two DNA templates using labeled primers (IBA Life Sciences). 120μg of each DNA template was digested with AflIII at 37°C overnight and purified by native PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... The Twin-Strep-tag® capture kit was from Iba Lifesciences (IBA GmbH, Germany). Peptides were purchased from Peptide 2.0 Inc (Chantilly ...
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bioRxiv - Microbiology 2020Quote: ... Primary CD4+ T cells were harvested by positive selection using a FABian automated enrichment system and CD4 isolation kit (IBA Lifesciences). Isolated T cells were suspended in complete RPMI-1640 medium supplemented with 5 mM HEPES ...