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Citations for IBA Life Sciences :
51 - 76 of 76 citations for Human Large Proline Rich Protein BAG6 BAG6 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... the tagged NStr-M35_FL or NStr-M35_S protein were purified by StrepTactin Superflow high capacity (IBA Lifesciences, Göttingen, Germany) chromatography with a 1 mL self-made column (Mobicol ...
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bioRxiv - Biochemistry 2023Quote: ... and the protein was purified from the supernatant by batch binding to Strep-Tactin XT Superflow resin (IBA Lifesciences). The resin was washed initially with Buffer A containing 0.05 % (w/v ...
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bioRxiv - Microbiology 2019Quote: ... Soluble proteins were loaded on self-prepared columns packed with Strep-Tactin XT superflow or Strep-Tactin Sepharose (IBA lifesciences) and purified under gravity flow ...
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bioRxiv - Biochemistry 2021Quote: ... Protein detection on the blots was performed with an antibody against the Strep-tag II (StrepMAB-Classic, IBA Lifesciences, Germany).
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bioRxiv - Microbiology 2021Quote: ... The protein was expressed and lysed as described above and purified according to the manufacturer’s guidelines (IBA Life Sciences, Inc.). Clarified lysates were passed over Strep-Tactin-Sepharose resin using a gravity filtration column ...
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bioRxiv - Molecular Biology 2022Quote: ... The column was washed until baseline and the protein was eluted with PBS containing 1 mM DTT and 2.5 mM desthiobiotin (IBA Lifesciences). Fractions were pooled based on the chromatogram and SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: ... 200 µg of stabilized histidine tagged S protein were labelled using Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and coated with 1 mg of magnetic beads (Dynabeads His-Tag ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was captured onto 2 mL of Strep-Tactin Superflow High-Capacity resin (IBA Lifesciences, cat. no. 2-1208-002) equilibrated with HEPES-Ca buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... Concentrated proteins were resuspended in buffer W at pH 8 and incubated with BioLock (#2-0205-050, Iba Life sciences) for 15 min at RT to neutralize biotin ...
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bioRxiv - Evolutionary Biology 2024Quote: ... the proteins were eluted in 1x BXT buffer (150 mM NaCl, 50 mM Tris-HCl pH 8.0, 1mM EDTA; IBA LifeSciences). We then exchanged the buffer to the protein buffer containing 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2024Quote: ... stabilized histidine-tagged SARS-CoV-2 S protein (Wuhan, XBB.1.5, BA.2.86) was labelled with Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and conjugated to magnetic beads according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... Beads were then washed with buffer B and proteins were eluted using buffer B supplemented with 10 mM desthiobiotin (IBA Lifesciences). Samples were taken at each step of the purification and loaded on SDS-PAGE gels to check the efficiency of affinity purification ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20 μL Strep-Tactin Macroprep beads (IBA lifesciences). Beads were washed three times with lysis buffer ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20-30 μl Strep-Tactin Macroprep beads (IBA lifesciences), FLAG or Ni-NTA beads (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleaved-off alkaline phosphatase and TEV protease were removed by re-purifying EXT1-EXT2 using a second HisTrap HP purification step and by passing protein sample through 1 mL Strep-Tactin XT resin (IBA Lifesciences). Buffer was exchanged by several rounds of concentration and dilution with buffer B (20mM HEPES pH 7.2 ...
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bioRxiv - Microbiology 2023Quote: ... supernatant from cells incubated with 4 µM CdCl2 for 5 days was collected and soluble protein was purified by affinity chromatography using a Strep-Tactin XT 4Flow column (IBA Lifesciences) followed by size exclusion chromatography using a HiLoad 26/600 superdex 200 pg column (Cytiva ...
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bioRxiv - Plant Biology 2023Quote: ... The elution fractions containing the trxA-14-3-3 or trxA-BIN2 fusion proteins were loaded onto a 5 ml Strep-Tactin Superflow High Capacity column (IBA Lifesciences), washed with buffer A and eluted in buffer A supplemented with 2.5 mM desthiobiotin ...
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bioRxiv - Microbiology 2023Quote: Secreted twin StrepTagged gp16-V1V2 proteins in the harvested and filtered supernatant were supplemented with BioLock biotin blocking solution (IBA Lifesciences) at 5 µl/ml to mask the biotin present in the supernatant ...
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bioRxiv - Biochemistry 2024Quote: ... Affinity purification of Strep3-tagged proteins was performed using a Strep-Tactin® Superflow® high capacity FPLC column (IBA Lifesciences).
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bioRxiv - Synthetic Biology 2022Quote: ... The solution was then centrifuged 3 times at 20,000 × g at 4 °C for 10 min periods and the supernatant was used for purification using fast protein liquid chromatography (FPLC) and 1 mL Strep-Tactin Superflow high capacity column (IBA Lifesciences, Germany). After equilibration with 5 column volume (CV ...
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bioRxiv - Immunology 2021Quote: ... Each culture media was collected and recombinant protein was purified from the supernatant using StrepTactin 4Flow® 5 mL cartridge (Iba Lifesciences) and buffers recommended by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was washed five times with IP buffer and eluted with Strep-Tactin elution buffer (IBA Life Sciences, 2-1000-025). Eluted samples were analyzed by western blot.
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bioRxiv - Cell Biology 2024Quote: ... The Twin-Strep-tag® capture kit was from Iba Lifesciences (IBA GmbH, Germany). Peptides were purchased from Peptide 2.0 Inc (Chantilly ...
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bioRxiv - Microbiology 2020Quote: ... Primary CD4+ T cells were harvested by positive selection using a FABian automated enrichment system and CD4 isolation kit (IBA Lifesciences). Isolated T cells were suspended in complete RPMI-1640 medium supplemented with 5 mM HEPES ...