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Citations for IBA Life Sciences :
1 - 50 of 126 citations for Human Fragile X Mental Retardation 1 Neighbor Protein FMR1NB ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: SEC purified HIV-1 Env-Soc trimers were tested for antigenicity using ELISA involving Microplates pre-coated with Strep-Tactin (IBA Life Sciences GmbH). Microplates were coated with 1 µg/ml SEC-purified gp140-Soc trimers in a volume of 100 µl/well of coating buffer (25 mM Tris-HCl ...
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bioRxiv - Immunology 2021Quote: ... 3C-digested proteins were loaded into StrepTactin XT 4Flow® 1 mL gravity flow columns (Iba Lifesciences) and collected in the flow-through for quantification ...
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bioRxiv - Molecular Biology 2022Quote: ... The column was washed until baseline and the protein was eluted with PBS containing 1 mM DTT and 2.5 mM desthiobiotin (IBA Lifesciences). Fractions were pooled based on the chromatogram and SDS-PAGE ...
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bioRxiv - Molecular Biology 2024Quote: ... and Twin-Strep tagged proteins of interest were purified from crude extracts (soluble fraction) using the Strep-Tactin®XT Superflow® Kit (IBA Lifesciences, Germany). Purification was performed according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20 μL Strep-Tactin Macroprep beads (IBA lifesciences). Beads were washed three times with lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20-30 μl Strep-Tactin Macroprep beads (IBA lifesciences), FLAG or Ni-NTA beads (Sigma) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleaved-off alkaline phosphatase and TEV protease were removed by re-purifying EXT1-EXT2 using a second HisTrap HP purification step and by passing protein sample through 1 mL Strep-Tactin XT resin (IBA Lifesciences). Buffer was exchanged by several rounds of concentration and dilution with buffer B (20mM HEPES pH 7.2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The solution was then centrifuged 3 times at 20,000 × g at 4 °C for 10 min periods and the supernatant was used for purification using fast protein liquid chromatography (FPLC) and 1 mL Strep-Tactin Superflow high capacity column (IBA Lifesciences, Germany). After equilibration with 5 column volume (CV ...
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bioRxiv - Immunology 2021Quote: ... Expressed protein was incubated with BioLock (IBA Lifesciences) and then isolated by Strep-tag affinity chromatography on StrepTrap HP columns (GE Healthcare) ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were eluted with 1X Buffer BX (IBA LifeSciences). Purified proteins were visualized on a BOLT 4-12% Bis-Tris Plus gel (Life Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were eluted with 1X Buffer BX (IBA LifeSciences) which contains 10 mM D-biotin ...
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bioRxiv - Biochemistry 2022Quote: ... Proteins were purified using Strep-Tactin Superflow resin (IBA Lifesciences) employing four washing steps ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble protein was loaded on Strep-TactinXT beads (IBA Lifesciences) and eluted with 50 mM Biotin (Applichem ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted protein was applied to Streptactin-XT resin (IBA lifesciences) equilibrated with equilibration buffer (100 mM Tris pH 8.0 ...
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bioRxiv - Cancer Biology 2023Quote: ... Human A3B-CTD and A3B-full were separately cloned into pASK-IBA5C plasmid vector (IBA LifeSciences) using Gibson cloning kit (ThermoFisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... lysates were clarified by centrifugation (17,000 x rpm for 40 min at 4 °C) and incubated with Strep-Tactin Sepharose (IBA Lifesciences) at 4 °C overnight ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified on Streptactin XT superflow beads (IBA Lifesciences) and eluted with 50 mM NaH2PO4.2H2O pH 8 ...
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bioRxiv - Biochemistry 2022Quote: ... The eluted protein was incubated with Strep-Tactin Sepharose (IBA Lifesciences) beads ...
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bioRxiv - Biophysics 2023Quote: ... the protein was purified using Streptactin affinity resin (IBA Life Sciences). Resin bound protein was washed as before and eluted using wash buffer ...
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bioRxiv - Immunology 2023Quote: ... The proteins were eluted using 1X BXT buffer (IBA Life Sciences) and buffer exchanged to 1X TBS using 100 kDa MWCO concentrators for trimers and 10 kDa MWCO concentrators for monomers (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... The protein was purified by using StrepTactin resin column (IBA Life sciences) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Purified proteins were then eluted by desthiobiotin using Buffer E (IBA Lifesciences) and subsequently prepared for immunoblotting as described above ...
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bioRxiv - Neuroscience 2023Quote: ... Final pure protein was eluted with Buffer BXT +10% glycerol (IBA Lifesciences) or BRB80 (80 mM PIPES-KOH ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble protein fraction was loaded on Strep-TactinXT beads (IBA Lifesciences) and eluted with 50 mM Biotin (Applichem ...
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bioRxiv - Immunology 2020Quote: ... Twin Strep- and His6-tagged proteins were captured on Streptactin XT (IBA LifeSciences) or Talon (Takara ...
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bioRxiv - Plant Biology 2023Quote: ... StrepII-tagged proteins were purified using Strep-Tactin MacroPrep (IBA Lifesciences, Göttingen, Germany) following the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... Strep protein was immunoprecipitated with 20 uL MagStrep “type3” XT beads (IBA Lifesciences) pre-washed with IP wash buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Microbiology 2021Quote: ... the protein was detected using the strep-tactin HRP conjugate (IBA Lifesciences, Göttingen, Germany) as specified by the manufacturer ...
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bioRxiv - Plant Biology 2023Quote: ... the eluted protein was next loaded onto a StrepTactin Sepharose column (IBA Life Sciences). The column was washed with buffer W (100 mM Tris pH 8.0 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Strep-tagged proteins were captured on Strep-Tactin XT Superflow High Capacity (IBA LifeSciences) affinity resin ...
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bioRxiv - Molecular Biology 2019Quote: ... The AQP7 protein was then purified by affinity chromatography using Strep-Tactin Superflow (IBA Lifesciences). The AQP7 was further purified with gel-filtration using superdex 200 10/300 Gel column (GE lifescience ...
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bioRxiv - Molecular Biology 2023Quote: ... CD4 proteins were purified using Ni2+-NTA (Cytiva) or Strep-Tactin XT (IBA Life Sciences) affinity columns ...
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bioRxiv - Plant Biology 2023Quote: ... The protein was purified with a Strep-Tactin XT 4Flow high-capacity column (IBA lifesciences) and stored in 10% glycerol in buffer W at -80°C ...
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bioRxiv - Molecular Biology 2022Quote: ... SARS-COV-2 NTD proteins were purified with Sepharose Strep-Tactin beads (IBA Life Sciences) as previously described [75].
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bioRxiv - Immunology 2024Quote: ... and recombinant proteins were purified using Strep-Tactin XT resin (IBA Lifesciences, Cat. #2-5010), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts were clarified and incubated with Strep-tactin® Superflow® resin (Iba Life science) at 4°C for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... Strep-tagged proteins were purified using affinity chromatography and Strep-Tactin® Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were expressed in BL21(DE3) and purified by Streptactin-XT resin (IBA Lifesciences, Göttingen, Germany) in the case of homodimers and Streptactin-XT resin and TALON resin (Takara Bio Inc. ...
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bioRxiv - Microbiology 2022Quote: ... Bound proteins were eluted for 30 minutes using 30 μL of BXT elution buffer (IBA Lifesciences) with constant shaking at room temperature ...
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bioRxiv - Immunology 2022Quote: ... The HA protein was purified from supernatants using StrepTactin-XT 4FLOW gravity flow columns (IBA Lifesciences). The protein was incubated with BXT elution buffer for 4 h ...
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bioRxiv - Microbiology 2023Quote: ... The strep tagged protein was purified using affinity chromatography and Strep-Tactin Sepharose (IBA Lifesciences, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The bound proteins were eluted with the binding buffer supplemented with 10 mM desthiobiotin (IBA Lifesciences). The samples were analyzed by SDS-PAGE and detected by immunoblotting using antibodies to the strep tag (1:3,000 ...
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bioRxiv - Microbiology 2022Quote: ... 2xStrep tagged S-protein was stained using StrepMAB-Classic DY-488 conjugate (IBA Lifesciences, 2-1563-050) and FLAG tagged S-protein was stained using anti-FLAG (clone M2 ...
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bioRxiv - Biochemistry 2022Quote: ... The S protein was eluted by Buffer A containing 0.02% DDM and 5 mM desthiobiotin (IBA Lifesciences). The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound proteins were eluted with the binding buffer supplemented with 10 mM desthiobiotin (IBA Lifesciences). The samples were analyzed by SDS-PAGE and detected by Coomassie staining.
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bioRxiv - Molecular Biology 2024Quote: ... jejuni 81-116) and Abu0127 proteins were purified according to the Strep-Tactin manufacturer’s protocol (IBA Lifesciences). Briefly ...
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bioRxiv - Cell Biology 2022Quote: ... StrepMAB-Classic (IBA LifeSciences, WB 1:2000, IF 1:500), StrepMAB-Classic DY-549 (IBA LifeSciences ...
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bioRxiv - Biochemistry 2020Quote: ... Viral proteins were affinity purified from 4mg lysates by tumbling with 40µl Strep-Tactin Sepharose (IBA Life Sciences) for 90min at 4°C ...
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bioRxiv - Developmental Biology 2022Quote: ... Elution fractions containing the Tmod proteins were then loaded onto Streptactin XT resin (IBA Life Sciences, Göttingen, Germany) and washed with 20 column volumes of strep buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting supernatant containing soluble proteins was loaded in Strep-Tactin XT 4Flow High-Capacity column (IBA lifesciences) previously saturated with N-TS-MtNCC1-78·Cu+ ...