Labshake search
Citations for IBA Life Sciences :
1 - 50 of 58 citations for Diazinon Unlabeled 1000 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... 2.5 mM desthiobiotin (IBA lifesciences, 2-1000-002). The eluted fractions containing highest concentration of the expressed protein were pooled and dialyzed against the storage buffer containing 10mM Tris/HCl ...
-
bioRxiv - Neuroscience 2021Quote: ... Twin-Strep-tag® (IBA Lifesciences; 2-1517-001; 1:1000); and Streptavidin 660 Conjugate (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2024Quote: ... a 5 mL polypropylene column was packed with 0.5 mL Strep-Tactin XT 4Flow resin (IBA Life Sciences), overlaid with 0.5 mL Strep wash buffer (100 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Systems Biology 2021Quote: ... 10 mL of lysis buffer (IBA LifeSciences Buffer W supplemented with 0.5% NP-40 substitute and one tablet of protease inhibitor (cOmplete™ EDTA-free Protease Inhibitor Cocktail ...
-
bioRxiv - Genetics 2023Quote: ... 1 ml StrepTactin (IBA Lifesciences, Göttingen, Germany) pre-equilibrated with lysis buffer ...
-
bioRxiv - Biophysics 2023Quote: ... 1.6 mL/L BioLock (IBA Lifesciences, Göttingen, Germany) was added to the supernatant and the pH was adjusted to 8.0 with 50 mM Tris pH 9.0 ...
-
bioRxiv - Molecular Biology 2023Quote: ... A 1.5 mL Streptactin (IBA Lifesciences, Göttingen, Germany) column was equilibrated in 100 mM Tris-HCl pH 8.5 ...
-
bioRxiv - Biochemistry 2022Quote: ... 1 mL of BioLock biotin blocking solution (IBA Lifesciences) were added to 1.5 L conditioned medium ...
-
bioRxiv - Plant Biology 2020Quote: ... They were next incubated in primary antibody solution for 2 hours at room temperature (1:1000 anti-Strep, IBA Life Sciences, 1:10,000 anti-FLAG M2 monoclonal antibody ...
-
bioRxiv - Microbiology 2020Quote: ... and incubated with 1 ml Strep-Tactin resin (IBA Lifesciences) for 2 hours at 4°C with rotation ...
-
bioRxiv - Microbiology 2024Quote: ... Bound protein was washed five times with IP buffer and eluted with Strep-Tactin elution buffer (IBA Life Sciences, 2-1000-025). Eluted samples were analyzed by western blot.
-
bioRxiv - Biochemistry 2023Quote: 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h gently stirring at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... The supernatant was mixed with 5 mL Strep-Tactin Sepharose (IBA Lifesciences) at 4 C overnight ...
-
bioRxiv - Molecular Biology 2021Quote: ... and subsequently applied to a 20 mL Strep-Tactin sepharose column (IBA Lifesciences). The affinity resin was washed (wash buffer ...
-
bioRxiv - Biophysics 2021Quote: ... The supernatant was incubated with 1 mL Streptactin sepharose beads (IBA Life Sciences) for 1 h ...
-
bioRxiv - Microbiology 2024Quote: ... the cleared lysate was incubated with 1.5 mL StrepTactin Sepharose beads (IBA Lifesciences) for 1 hour at 4°C on a rotator ...
-
bioRxiv - Biochemistry 2021Quote: ... and the supernatant was loaded on a 1-mL Strep-Tactin column (IBA Lifesciences). Purification was performed as specified by the manufacturer ...
-
bioRxiv - Biochemistry 2019Quote: ... The supernatant was incubated with 5 mL of equilibrated StrepTactin Superflow resin (IBA Lifesciences). The resin was washed and equilibrated with Buffer A containing 0.02% DDM and 0.004% CHS ...
-
bioRxiv - Biochemistry 2020Quote: ... The eluate was loaded on a 5 mL StrepTactin XP column (IBA life sciences), washed with 25 mL Strep-A buffer (150 mM NaCl ...
-
bioRxiv - Biochemistry 2023Quote: ... Affinity chromatography was performed with Strep-Tactin Sepharose slurry (1 mL/L) (IBA Lifesciences), using 1xTris buffer (25 mM Tris-HCl pH 8.0 ...
-
bioRxiv - Biochemistry 2023Quote: Lysate was loaded on 5 ml of Streptactin-XT resin (IBA Lifesciences, Göttingen, Germany) and passed through the resin by gravity flow ...
-
bioRxiv - Microbiology 2024Quote: ... Cleared lysates were then incubated with 1.5 mL of StrepTactin Sepharose beads (IBA Lifesciences) for 1 hour at 4°C on a rotator ...
-
bioRxiv - Biophysics 2020Quote: ... The lysate was mixed with 2 mL Strep-Tactin XT resin (IBA Lifesciences, Göttingen, Germany). The resin was washed extensively with wash buffer (50 mM Tris ...
-
bioRxiv - Cell Biology 2019Quote: ... The lysate was mixed with 2 ml Strep-Tactin XT resin (IBA Lifesciences, Göttingen, Germany). The resin was washed with wash buffer (50 mM Tris ...
-
bioRxiv - Biochemistry 2023Quote: ... A 1 ml Strep-Tactin XT 4Flow high-capacity cartridge (IBA Lifesciences 2-5027-001) was washed with 14 column volumes of wash buffer (50 mM Hepes pH 7.6 ...
-
bioRxiv - Biochemistry 2023Quote: ... Expression was then induced with anhydrotetracycline (final concentration 200 ng/mL; IBA Life Sciences, Germany) and carried out for 16 h at 20 °C and 1050 rpm shaking ...
-
bioRxiv - Immunology 2021Quote: ... and further purified using a 2 mL StrepTactin sepharose column (IBA Lifesciences Cat# 2-1201-002) and a Strep-Tactin Purification Buffer Set (IBA Lifesciences Cat#2-1002-001) ...
-
bioRxiv - Biophysics 2022Quote: ... before being loaded into a 6 mL Strep-Tactin Superflow high capacity resin (IBA Lifesciences, USA). The resin was washed using 6 column volumes of Buffer W1 (20 mM HEPES pH 7.5 ...
-
bioRxiv - Biochemistry 2023Quote: ... or 2 mL of Strep-Tactin® Sepharose® resin (IBA Lifesciences GmbH, #2-1201-002) for strep-II tagged proteins ...
-
bioRxiv - Immunology 2021Quote: ... 3C-digested proteins were loaded into StrepTactin XT 4Flow® 1 mL gravity flow columns (Iba Lifesciences) and collected in the flow-through for quantification ...
-
bioRxiv - Microbiology 2022Quote: ... Bacteria were plated on 7H10 plates containing kanamycin and 100 ng/ml anhydrotetracycline (ATc, IBA Life sciences) for the induction of the S ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cleared extract was passed through 1 mL Strep - Tactin XT Superflow (IBA Lifesciences GmbH, Göttingen, Germany) column pre-equilibrated with 250C buffer (25 mM HEPES - KOH pH 7.6 ...
-
bioRxiv - Plant Biology 2023Quote: ... 1 mM EDTA) and loaded onto a 5 ml Strep-Tactin Superflow High Capacity colum (IBA Lifesciences). Proteins were eluted from the column in Strep buffer supplement with 2.5 mM desthiobiotin followed by over-night TEV cleavage of the N-terminal affinity tag for 16 h at 4 °C during dialysis against Strep buffer ...
-
bioRxiv - Biochemistry 2023Quote: ... After equilibration of the Strep-tactin XT Superflow resin (IBA lifesciences, 10 mL per liter of cell supernatant) in 0.1 M Tris-HCl pH 8 ...
-
bioRxiv - Microbiology 2023Quote: ... membranes were incubated with StrepMAB-Classic monoclonal antibody conjugated to HRP (0.05 µg/mL, #2-1509-001, Iba Lifesciences) for 2 h at RT ...
-
bioRxiv - Microbiology 2023Quote: ... Strep-Tactin XT resin (1 mL) was prepared following the manufacturer’s instructions for gravity flow purification (Iba Life Sciences). After loading the cleared lysate ...
-
bioRxiv - Molecular Biology 2023Quote: ... The complex was eluted in five 1 mL fractions using buffer C with added 5 mM desthiobiotin (IBA Lifesciences). After addition of TCEP to 1 mM ...
-
bioRxiv - Plant Biology 2020Quote: The supernatant was filtered through a 0.45 µm filter and applied to a 5 ml Strep-Tactin Superflow HC column (IBA Lifesciences), equilibrated in Buffer W (100 mM Tris-HCL ...
-
bioRxiv - Microbiology 2023Quote: ... The supernatant was subjected to affinity chromatography using 1 mL buffer equilibrated Strep-Tactin XT 4Flow resin (IBA Lifesciences-GmbH) and flow through was collected ...
-
bioRxiv - Molecular Biology 2023Quote: ... The supernatant was filtered through a 5.0 µm syringe filter followed by 0.45 µm syringe filters and loaded on to 3 mL gravity flow column packed with StrepTactin Sepharose resin (IBA Lifesciences). The column was washed with wash buffer (20 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Biochemistry 2022Quote: ... Protein was captured onto 2 mL of Strep-Tactin Superflow High-Capacity resin (IBA Lifesciences, cat. no. 2-1208-002) equilibrated with HEPES-Ca buffer (20 mM HEPES pH 7.4 ...
-
bioRxiv - Molecular Biology 2021Quote: ... TWIN-Strep tagged proteins were then purified by incubating whole cell lysates from the transiently-transfected cell lines (0.5 ml) with 50 μl of MagStrep “type3” XT beads (IBA Life Sciences) for two-four hours at 4°C ...
-
bioRxiv - Microbiology 2020Quote: Purified StrepII-tagged sE2 monomer (1.0 µg/mL in TBS) was coated for 2 hour at room temperature on 96-well Strep-TactinXT coated microplates (IBA LifeSciences). Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems ...
-
bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
-
bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
-
bioRxiv - Cancer Biology 2022Quote: ... PBMCs from HLA-A*0201 Donors were either stimulated with 1 µg/mL CMV pp65 antigen peptide NLVPMVATV (HLA-A*0201) (IBA Lifesciences) for 1 h or not ...
-
bioRxiv - Molecular Biology 2022Quote: ... Cleaved-off alkaline phosphatase and TEV protease were removed by re-purifying EXT1-EXT2 using a second HisTrap HP purification step and by passing protein sample through 1 mL Strep-Tactin XT resin (IBA Lifesciences). Buffer was exchanged by several rounds of concentration and dilution with buffer B (20mM HEPES pH 7.2 ...
-
bioRxiv - Synthetic Biology 2023Quote: ... Gravity flow columns (2 mL) were prepared with 400 μL 50% Strep-Tactin® XT Superflow High Capacity suspension (IBA Lifesciences). Protein purification was performed according to manufacturer’s protocol ...
-
bioRxiv - Plant Biology 2023Quote: ... The elution fractions containing the trxA-14-3-3 or trxA-BIN2 fusion proteins were loaded onto a 5 ml Strep-Tactin Superflow High Capacity column (IBA Lifesciences), washed with buffer A and eluted in buffer A supplemented with 2.5 mM desthiobiotin ...
-
bioRxiv - Biochemistry 2023Quote: Expression of each mutant in the membrane samples was evaluated through western blotting using 10 ng mL-1 Strep-Tactin conjugated with horseradish peroxidase (IBA Lifesciences) and using SYPRO Ruby (Thermo Fisher Scientific ...