Labshake search
Citations for IBA Life Sciences :
1 - 50 of 97 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: ... The Twin-Strep tag and Strep-Tactin XT protein fluorescently conjugated with DY-649 were purchased from IBA Lifesciences.
-
bioRxiv - Biophysics 2022Quote: ... The proteins were purified via a C-terminal STREP II tag using Strep-tactin resin (IBA LifeSciences, Göttingen, DE). The solubilizing detergents were replaced by 0.06% (w/v ...
-
bioRxiv - Biochemistry 2023Quote: ... the SNAPf-EGFP-6His tag was replaced with a strep-II tag (IBA Lifesciences GmbH) using Q5 mutagenesis (New England Biolabs Inc. ...
-
bioRxiv - Biochemistry 2021Quote: ... Protein detection on the blots was performed with an antibody against the Strep-tag II (StrepMAB-Classic, IBA Lifesciences, Germany).
-
bioRxiv - Biochemistry 2024Quote: ... a Twin-Strep-tag (IBA Lifesciences, Göttingen) and ARID49-152 ...
-
bioRxiv - Biochemistry 2023Quote: ... Histones H2A.S2 (bearing a C-terminal StrepII-tag, IBA Lifesciences) and H2B were expressed individually ...
-
bioRxiv - Cell Biology 2022Quote: ... StrepMAB-Classic DY-549 (IBA LifeSciences, FC 1:500), Strep-Tactin-DY649 (IBA LifeSciences ...
-
bioRxiv - Neuroscience 2021Quote: ... Twin-Strep-tag® (IBA Lifesciences; 2-1517-001; 1:1000); and Streptavidin 660 Conjugate (ThermoFisher Scientific ...
-
bioRxiv - Molecular Biology 2022Quote: ... using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences). Subsequently ...
-
bioRxiv - Immunology 2019Quote: ... or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001) and anti-mouse IgG1 antibody Alexa Fluor 647 (Thermo Fisher Scientific # A-21240) ...
-
bioRxiv - Immunology 2023Quote: ... or anti-Strep-tag II antibody (IBA Lifesciences #2-1507-001) and anti-mouse IgG1 antibody Alexa Fluor 647 (Thermo Fisher Scientific #A-21240) ...
-
bioRxiv - Bioengineering 2021Quote: ... Strep-tag and Strep-Tactin XT were purchased from IBA Lifesciences (Gottingen, Germany). Detergent compatible (DC ...
-
bioRxiv - Biophysics 2022Quote: ... Mouse monoclonal antibody against the Strep tag (StrepMAB-Classic) was from IBA Lifesciences (Catalog # ...
-
bioRxiv - Immunology 2020Quote: ... The (Twin)-Strep-tag /Strep-Tactin affinity purification system (IBA LifeSciences, Göttingen, Germany) was used to produce twin-strep-tagged fusion chemokines which were detected with an HRP-conjugated anti-Streptag mAb (IBA LifeSciences) ...
-
bioRxiv - Biochemistry 2019Quote: Fluorescently tagged Arc and StrepII tagged Arc were coexpressed in HEK293 cells and purified using Strep-Tactin Sepharose (IBA Lifesciences). Formation of complexes between the indicated Arc constructs was analyzed by immunoblotting following SDS electrophoresis ...
-
bioRxiv - Cell Biology 2024Quote: ... The Twin-Strep-tag® capture kit was from Iba Lifesciences (IBA GmbH, Germany). Peptides were purchased from Peptide 2.0 Inc (Chantilly ...
-
bioRxiv - Biochemistry 2024Quote: ... the ZnF was expressed as a fusion construct with an N-terminal Twin-Strep-tag® (IBA Lifesciences, Göttingen) and purified as the other constructs.
-
bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: StrepMAB-Immo (anti-Twin-Strep-tag®; IBA Lifesciences; 2-1517-001; 1:4000), FUS (1:2000 ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... Membranes were rinsed once with PBS-T (PBS with 0.01% Tween-20) and incubated with primary Strep-tag II antibody (Strep-MAB-Classic, IBA Lifesciences, Cat# 2-1507-001 ...
-
bioRxiv - Biochemistry 2022Quote: ... For that purpose VI23.60 containing Strep-tag® was immobilized on the Strep-Tactin® coated microplate (IBA Lifesciences GmbH, Germany) and three rounds of selection were performed ...
-
bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated either with anti-strep tag antibody (IBA Lifesciences) or anti-SARS-COV-2 polyclone antibody (Sino Biological Inc. ...
-
bioRxiv - Microbiology 2020Quote: ... coli MG1655 genomic DNA with primers 5’ CTC GGT CTC CAA TGA ATA AAT ATC AGG CAG TGA 3’ and 5’ CTC GGT CTC AGC GCT TTA TTT GAC TAA TGA AAA TAG ATC A 3’ and cloned into the Eco31I (BsaI) sites of plasmid pPR-IBA101 (IBA Lifesciences) to yield plasmid pRCLA10 ...
-
bioRxiv - Biophysics 2019Quote: ... APC/C was expressed with a Twin-Strep(II)-tag on the C-terminus of APC4 and purified using Strep-Tactin Sepharose (IBA Life Sciences) affinity chromatography followed by ion exchange chromatography and SEC ...
-
bioRxiv - Immunology 2019Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences # 2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001 ...
-
bioRxiv - Microbiology 2022Quote: ... incubated with anti-StrepMAB-Classic-HRP antibody that recognizes the strep tag at the C-terminus of the SFV Env ectodomain (7.5 μg/ml, IBA Lifesciences #2-1509-001) for 1 hour ...
-
bioRxiv - Immunology 2023Quote: Cells were analysed for receptor surface expression by flow cytometry using anti-Strep-tag II antibody Oyster 645 (IBA Lifesciences #2-1555-050), or anti-Strep-tag II antibody (IBA Lifesciences #2-1507-001 ...
-
bioRxiv - Biochemistry 2024Quote: ... The complex was purified by pulling down from the Strep-tag in AtRPAP3 with a StrepTactin®XT 4Flow® column (IBA Lifesciences) using the same protocol described for AtRPAP3 ...
-
bioRxiv - Biophysics 2019Quote: ... the C-terminal His tag was cleaved by treatment with TEV protease and passed over the Ni-NTA and Strep-Tactin XT Superflow (IBA Life Sciences, Göttingen, Germany) columns attached in tandem ...
-
bioRxiv - Immunology 2021Quote: ... Expressed protein was incubated with BioLock (IBA Lifesciences) and then isolated by Strep-tag affinity chromatography on StrepTrap HP columns (GE Healthcare) ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins were eluted with 1X Buffer BX (IBA LifeSciences). Purified proteins were visualized on a BOLT 4-12% Bis-Tris Plus gel (Life Technologies ...
-
bioRxiv - Molecular Biology 2021Quote: ... Proteins were eluted with 1X Buffer BX (IBA LifeSciences) which contains 10 mM D-biotin ...
-
bioRxiv - Biochemistry 2022Quote: ... Proteins were purified using Strep-Tactin Superflow resin (IBA Lifesciences) employing four washing steps ...
-
bioRxiv - Biochemistry 2022Quote: ... Soluble protein was loaded on Strep-TactinXT beads (IBA Lifesciences) and eluted with 50 mM Biotin (Applichem ...
-
bioRxiv - Biochemistry 2023Quote: ... Eluted protein was applied to Streptactin-XT resin (IBA lifesciences) equilibrated with equilibration buffer (100 mM Tris pH 8.0 ...
-
bioRxiv - Cancer Biology 2023Quote: ... Human A3B-CTD and A3B-full were separately cloned into pASK-IBA5C plasmid vector (IBA LifeSciences) using Gibson cloning kit (ThermoFisher Scientific) ...
-
bioRxiv - Microbiology 2021Quote: ... The protein was purified on Streptactin XT superflow beads (IBA Lifesciences) and eluted with 50 mM NaH2PO4.2H2O pH 8 ...
-
bioRxiv - Biochemistry 2022Quote: ... The eluted protein was incubated with Strep-Tactin Sepharose (IBA Lifesciences) beads ...
-
bioRxiv - Biophysics 2023Quote: ... the protein was purified using Streptactin affinity resin (IBA Life Sciences). Resin bound protein was washed as before and eluted using wash buffer ...
-
bioRxiv - Immunology 2023Quote: ... The proteins were eluted using 1X BXT buffer (IBA Life Sciences) and buffer exchanged to 1X TBS using 100 kDa MWCO concentrators for trimers and 10 kDa MWCO concentrators for monomers (Millipore) ...
-
bioRxiv - Microbiology 2021Quote: ... The protein was purified by using StrepTactin resin column (IBA Life sciences) following the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2023Quote: ... Purified proteins were then eluted by desthiobiotin using Buffer E (IBA Lifesciences) and subsequently prepared for immunoblotting as described above ...
-
bioRxiv - Neuroscience 2023Quote: ... Final pure protein was eluted with Buffer BXT +10% glycerol (IBA Lifesciences) or BRB80 (80 mM PIPES-KOH ...
-
bioRxiv - Biochemistry 2023Quote: ... The soluble protein fraction was loaded on Strep-TactinXT beads (IBA Lifesciences) and eluted with 50 mM Biotin (Applichem ...
-
bioRxiv - Immunology 2020Quote: ... Twin Strep- and His6-tagged proteins were captured on Streptactin XT (IBA LifeSciences) or Talon (Takara ...
-
bioRxiv - Plant Biology 2023Quote: ... StrepII-tagged proteins were purified using Strep-Tactin MacroPrep (IBA Lifesciences, Göttingen, Germany) following the manufacturer’s instructions.
-
bioRxiv - Cell Biology 2024Quote: ... Strep protein was immunoprecipitated with 20 uL MagStrep “type3” XT beads (IBA Lifesciences) pre-washed with IP wash buffer (50 mM Tris-HCl pH 7.4 ...
-
bioRxiv - Microbiology 2021Quote: ... the protein was detected using the strep-tactin HRP conjugate (IBA Lifesciences, Göttingen, Germany) as specified by the manufacturer ...
-
bioRxiv - Plant Biology 2023Quote: ... the eluted protein was next loaded onto a StrepTactin Sepharose column (IBA Life Sciences). The column was washed with buffer W (100 mM Tris pH 8.0 ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Strep-tagged proteins were captured on Strep-Tactin XT Superflow High Capacity (IBA LifeSciences) affinity resin ...
-
bioRxiv - Molecular Biology 2019Quote: ... The AQP7 protein was then purified by affinity chromatography using Strep-Tactin Superflow (IBA Lifesciences). The AQP7 was further purified with gel-filtration using superdex 200 10/300 Gel column (GE lifescience ...