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Citations for IBA Life Sciences :
1 - 50 of 96 citations for 7 Deaza 2' deoxyadenosine 5' O monophosphate 7 CH 5' dAMP dTuMP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 5% suspension (IBA Lifesciences) and eluted with desthiobiotin ...
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bioRxiv - Biochemistry 2019Quote: The BBS2-7-9 complex was purified at 4°C by affinity purification using 4 mL of Strep-Tactin resin (IBA Life Sciences) packed in a 2 cm diameter column and equilibrated with equilibration buffer (20 mM HEPES pH 7.5 and 20 mM NaCl) ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli cells (5-1600-020, IBA Life Sciences, Göttingen). Transformants were screened by colony PCR and verified by sequencing (StabVida ...
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bioRxiv - Biochemistry 2024Quote: ... 5 min) and incubated on ice for 30 min with 100 nM scFv and StrepTactin-XT DY647 conjugate (IBA Lifesciences, 2-1568-050) at a 1:200 dilution in 1% BSA in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... from 0 mM to 5 mM desthiobiotin (IBA Lifesciences GmbH), was then used to elute each complex ...
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bioRxiv - Biophysics 2021Quote: ... The TS-SUMO-SaCas9-sgRNA or TS-SUMO-SaCas9 cell lysate was applied to a 5 mL Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer containing 200 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was mixed with 5 mL Strep-Tactin Sepharose (IBA Lifesciences) at 4 C overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... hnRNPH1: 5′-CUCUUGUCCAUCUAGAC-3′] used for the NMR titration was purchased (IBA Lifesciences).
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bioRxiv - Biochemistry 2019Quote: ... The supernatant was incubated with 5 mL of equilibrated StrepTactin Superflow resin (IBA Lifesciences). The resin was washed and equilibrated with Buffer A containing 0.02% DDM and 0.004% CHS ...
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bioRxiv - Biochemistry 2020Quote: ... The eluate was loaded on a 5 mL StrepTactin XP column (IBA life sciences), washed with 25 mL Strep-A buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: Lysate was loaded on 5 ml of Streptactin-XT resin (IBA Lifesciences, Göttingen, Germany) and passed through the resin by gravity flow ...
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bioRxiv - Biochemistry 2020Quote: ... LMNG-solubilized lysates were added to 5 μg of TBS-prewashed MagStrep XT magnetic beads (IBA Lifesciences) for 1 h with mild shaking ...
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bioRxiv - Biochemistry 2022Quote: ... The S protein was eluted by Buffer A containing 0.02% DDM and 5 mM desthiobiotin (IBA Lifesciences). The protein was further purified by gel filtration chromatography on a Superose 6 10/300 column (GE Healthcare ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM EDTA) and loaded onto a 5 ml Strep-Tactin Superflow High Capacity colum (IBA Lifesciences). Proteins were eluted from the column in Strep buffer supplement with 2.5 mM desthiobiotin followed by over-night TEV cleavage of the N-terminal affinity tag for 16 h at 4 °C during dialysis against Strep buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... a 5 mL polypropylene column was packed with 0.5 mL Strep-Tactin XT 4Flow resin (IBA Life Sciences), overlaid with 0.5 mL Strep wash buffer (100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... Culture supernatants were harvested 5 days post-transfection and purified with sepharose strep-tactin beads (IBA Life Sciences, Germany) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... Culture supernatants were harvested 5 days post transfection and purified with sepharose strep-tactin beads (IBA Life Sciences, Germany) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... The complex was eluted in five 1 mL fractions using buffer C with added 5 mM desthiobiotin (IBA Lifesciences). After addition of TCEP to 1 mM ...
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bioRxiv - Biochemistry 2020Quote: ... LMNG and proteins were eluted in 5 CV of TBS supplemented with 0.01% (w/v) LMNG and 50 mM D-biotin (IBA Lifesciences). Eluates were concentrated using a 100-kDa molecular weight cutoff (MWCO ...
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bioRxiv - Plant Biology 2020Quote: The supernatant was filtered through a 0.45 µm filter and applied to a 5 ml Strep-Tactin Superflow HC column (IBA Lifesciences), equilibrated in Buffer W (100 mM Tris-HCL ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated either with anti-strep tag antibody (IBA Lifesciences) or anti-SARS-COV-2 polyclone antibody (Sino Biological Inc. ...
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bioRxiv - Immunology 2021Quote: ... Recombinant proteins were purified from each collected supernatant by affinity chromatography using StrepTactin XT 4Flow® 5 mL cartridges (Iba Lifesciences). Eluted RBDQ498Y and ACE2 were then concentrated and buffer-exchanged to TBS pH 7.4 using 10 kDa and 50 kDa 4 mL Amicons (Merck) ...
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bioRxiv - Biochemistry 2022Quote: ... the lysate was clarified by centrifugation and SBP-tagged proteins were affinity purified using 5 ml of Streptactin superflow resin (IBA LifeSciences). The resin was washed with 40 column volumes (CV ...
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bioRxiv - Microbiology 2023Quote: ... supernatant from cells incubated with 4 µM CdCl2 for 5 days was collected and soluble protein was purified by affinity chromatography using a Strep-Tactin XT 4Flow column (IBA Lifesciences) followed by size exclusion chromatography using a HiLoad 26/600 superdex 200 pg column (Cytiva ...
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bioRxiv - Plant Biology 2023Quote: ... The elution fractions containing the trxA-14-3-3 or trxA-BIN2 fusion proteins were loaded onto a 5 ml Strep-Tactin Superflow High Capacity column (IBA Lifesciences), washed with buffer A and eluted in buffer A supplemented with 2.5 mM desthiobiotin ...
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bioRxiv - Biochemistry 2023Quote: ... Constructs were then added to pre-washed (PBST) and pre-blocked (5% BSA in PBST) magnetic streptavidin beads (Dynabeads M-280, Thermo) or Streptactin-agarose (IBA Lifesciences), with the amount of beads used being tenfold greater than what would be theoretically required based on the manufacturer’s listed binding capacity ...
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bioRxiv - Cell Biology 2019Quote: The custom 20-mer oligonucleotide modified with dibenzocyclooctyne (DBCO) (Sequence: 5’ - TCA GTT CAG GAC CCT CGG CT – DBCO – 3’) was purchased from IBA Life Sciences, reconstituted in nuclease free water at a concentration of 600 μM and stored at −20°C ...
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bioRxiv - Immunology 2021Quote: ... Each culture media was collected and recombinant protein was purified from the supernatant using StrepTactin 4Flow® 5 mL cartridge (Iba Lifesciences) and buffers recommended by the manufacturer ...
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bioRxiv - Immunology 2021Quote: ... and further purified using a 2 mL StrepTactin sepharose column (IBA Lifesciences Cat# 2-1201-002) and a Strep-Tactin Purification Buffer Set (IBA Lifesciences Cat#2-1002-001) ...
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bioRxiv - Biochemistry 2023Quote: ... or 2 mL of Strep-Tactin® Sepharose® resin (IBA Lifesciences GmbH, #2-1201-002) for strep-II tagged proteins ...
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bioRxiv - Microbiology 2021Quote: ... Samples stained with trimeric SARS-CoV-2 RBD were incubated with primary antibody strepmabHRP (2-1509-001, IBA lifesciences) with specificity towards the TwinStrep-tag at the C-terminus of the trimeric protein for 120 hours in PBSGT with 0.1% saponin ...
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bioRxiv - Biochemistry 2022Quote: ... Protein was captured onto 2 mL of Strep-Tactin Superflow High-Capacity resin (IBA Lifesciences, cat. no. 2-1208-002) equilibrated with HEPES-Ca buffer (20 mM HEPES pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... 2.5 mM desthiobiotin (IBA lifesciences, 2-1000-002). The eluted fractions containing highest concentration of the expressed protein were pooled and dialyzed against the storage buffer containing 10mM Tris/HCl ...
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bioRxiv - Neuroscience 2021Quote: ... Twin-Strep-tag® (IBA Lifesciences; 2-1517-001; 1:1000); and Streptavidin 660 Conjugate (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences), and SARS-CoV-2 spike-StreptTactin APC (StrepTactin APC from IBA Lifesciences).
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences), SARS-CoV-2 ΔRBM RBD-APC (streptavidin-APC from BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 spike-StreptTactin PE (StrepTactin PE from IBA Lifesciences), SARS-CoV-2 ΔRBM RBD-PE/Cy5.5 (streptavidin-PE/Cy5.5 from BioLegend) ...
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bioRxiv - Immunology 2019Quote: ... or anti-Strep-tag II antibody (IBA Lifesciences # 2-1507-001) and anti-mouse IgG1 antibody Alexa Fluor 647 (Thermo Fisher Scientific # A-21240) ...
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bioRxiv - Genetics 2021Quote: ... streptactin DY-488 (IBA Lifesciences; Cat. # 2-1562-050; 1:500) was added to secondary antibody solution ...
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bioRxiv - Microbiology 2022Quote: ... StrepMAB-Classic (1:500, mouse monoclonal, IBA lifesciences, 2-1507-001), anti-SARS-CoV-2 Spike (1:100 ...
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bioRxiv - Immunology 2023Quote: ... or anti-Strep-tag II antibody (IBA Lifesciences #2-1507-001) and anti-mouse IgG1 antibody Alexa Fluor 647 (Thermo Fisher Scientific #A-21240) ...
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bioRxiv - Biochemistry 2024Quote: ... and purified on StrepTactin-XT resin (IBA Lifesciences, 2-5010-002) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and SARS-CoV-2 spike-StreptTactin APC (StrepTactin APC from IBA Lifesciences).
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bioRxiv - Immunology 2020Quote: ... and Strep-Tactin Purification Buffer Set (IBA Lifesciences Cat # 2-1002-001) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h gently stirring at 4°C ...
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bioRxiv - Immunology 2023Quote: ... and S1-StrepTactin XT DY-649 (IBA life sciences, 2-1568-050) and S1-StrepTactin XT DY-488 (IBA life sciences ...
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bioRxiv - Immunology 2023Quote: ... and S1-StrepTactin XT DY-488 (IBA life sciences, 2-1562- 050). Live CD3/CD8-CD14-CD19+IgM-IgD-IgG+S1+S1+ cells were sorted using a BD FACS Melody into individual wells containing RNase OUT (Invitrogen) ...
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bioRxiv - Cell Biology 2023Quote: ... The Strep-Tactin Superflow high-capacity resin (IBA lifesciences, 2-1208-002) was used to purify the protein ...
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bioRxiv - Neuroscience 2021Quote: ... Cell lysate was immunoprecipitated with Magstrep Type3 beads (IBA Lifesciences; 2-4090-002) overnight with end/end rocking at 4°C following the protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... and applied to Strep-Tactin affinity chromatography column (IBA Lifesciences, #2-1201-025) according to the manufacturer’s protocol ...