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Citations for IBA Life Sciences :
1 - 50 of 91 citations for 6H Dibenzo b d pyran 3 pentanaminium 6a 7 10 10a tetrahydro 1 hydroxy N N N 6 6 9 hexamethyl 6aR trans 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... the ZnF was expressed as a fusion construct with an N-terminal Twin-Strep-tag® (IBA Lifesciences, Göttingen) and purified as the other constructs.
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bioRxiv - Biochemistry 2023Quote: ... The column was washed with 5 volumes of equilibration buffer and eluted with 6 volumes of the same buffer supplemented with 50 mM D-biotin (IBA lifesciences). The fractions containing Mini-I were pooled and concentrated by ultrafiltration (Vivaspin 20 ...
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bioRxiv - Immunology 2021Quote: Transfected cells were sorted for HDR+ by staining for Strep-Tactin-APC 1:100 (IBA lifesciences, Cat: 6-5010-001) and anti-hIgG AF488 1:100 (Jackson ImmunoResearch ...
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bioRxiv - Biochemistry 2019Quote: The BBS2-7-9 complex was purified at 4°C by affinity purification using 4 mL of Strep-Tactin resin (IBA Life Sciences) packed in a 2 cm diameter column and equilibrated with equilibration buffer (20 mM HEPES pH 7.5 and 20 mM NaCl) ...
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bioRxiv - Microbiology 2022Quote: ... Beads were then washed with buffer B and proteins were eluted using buffer B supplemented with 10 mM desthiobiotin (IBA Lifesciences). Samples were taken at each step of the purification and loaded on SDS-PAGE gels to check the efficiency of affinity purification ...
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bioRxiv - Biochemistry 2021Quote: ... were formed as described previously (Sidorenkov et al., 1998) using scaffold 1 (Table 6) and the RNA sequence: AUCGAGAGG (IBA life sciences). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... before being loaded into a 6 mL Strep-Tactin Superflow high capacity resin (IBA Lifesciences, USA). The resin was washed using 6 column volumes of Buffer W1 (20 mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... Cell culture supernatant was harvested 6 days after transfection and purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2020Quote: ... The resin was then washed 3 times with RIPA buffer and eluted with 1’ Strep-Tactin elution buffer with biotin (IBA Life Sciences). The input ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM TCEP) with pre-equilibrated magnetic Strep-Tactin XT beads (240 µl MagStrep type 3 XT bead slurry, IBA life sciences). Following 1 h of binding at room temperature on a rotator ...
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bioRxiv - Plant Biology 2023Quote: ... The elution fractions containing the trxA-14-3-3 or trxA-BIN2 fusion proteins were loaded onto a 5 ml Strep-Tactin Superflow High Capacity column (IBA Lifesciences), washed with buffer A and eluted in buffer A supplemented with 2.5 mM desthiobiotin ...
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bioRxiv - Immunology 2024Quote: ... B cells were stained with rGP38 (IbAr10200) that had been tetramerized at 25 nM using Streptactin-PE (IBA Lifesciences) and Streptactin-APC (IBA Lifesciences) ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble membrane fraction was diluted 2-fold with buffer B and applied to a column packed with Strep-Tactin beads (IBA Lifesciences) by gravity flow (6–10 s/drop ...
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bioRxiv - Biochemistry 2023Quote: Expression of each mutant in the membrane samples was evaluated through western blotting using 10 ng mL-1 Strep-Tactin conjugated with horseradish peroxidase (IBA Lifesciences) and using SYPRO Ruby (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2022Quote: ... and 10% Buffer BXT (IBA Lifesciences) and gently rotated for 1 h at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... hnRNPH1: 5′-CUCUUGUCCAUCUAGAC-3′] used for the NMR titration was purchased (IBA Lifesciences).
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bioRxiv - Systems Biology 2021Quote: ... 10 mL of lysis buffer (IBA LifeSciences Buffer W supplemented with 0.5% NP-40 substitute and one tablet of protease inhibitor (cOmplete™ EDTA-free Protease Inhibitor Cocktail ...
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was performed with MagStrep type 3 XT beads (Iba Life Sciences, Gottingen, Germany) according to the manufacturers protocol ...
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bioRxiv - Biochemistry 2021Quote: ... The SBP-FKBP12.6-RyR2 complex was eluted with the wash buffer supplemented with 2.5 mM D-desthiobiotin (Iba Lifesciences). After checking the purity by SDS-PAGE ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was added to magnetic Strep beads (MagStrep type 3 beads, IBA Life science) previously equilibrated with buffer A and incubated for 30 min at 4 °C on a roller ...
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bioRxiv - Cancer Biology 2022Quote: ... Washed magnetic Streptactin beads (100 μl, MagStrep type 3 XT, IBA Life Sciences, Gottingen, Germany) were added and incubated overnight at 4°C with agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... LMNG and proteins were eluted in 5 CV of TBS supplemented with 0.01% (w/v) LMNG and 50 mM D-biotin (IBA Lifesciences). Eluates were concentrated using a 100-kDa molecular weight cutoff (MWCO ...
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bioRxiv - Genomics 2020Quote: ... 800 μL supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4ºC ...
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bioRxiv - Molecular Biology 2023Quote: ... 800 µL supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... and then 1mg of lysate was incubated with prewashed MagStrep “type 3” XT magnetic beads (IBA LifeSciences) overnight in IP buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... The bound protein was eluted in 3 CV buffer W supplemented with 2.5 mM desthiobiotin (IBA Life Sciences). This material was then applied to a MonoQ 5/50 GL (Cytiva ...
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bioRxiv - Neuroscience 2023Quote: ... Final pure protein was eluted with Buffer BXT +10% glycerol (IBA Lifesciences) or BRB80 (80 mM PIPES-KOH ...
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bioRxiv - Molecular Biology 2021Quote: ... 2xStrep tagged SARS-CoV-2 ORFs were immunoprecipitated using 30 μl of Megastrep type 3 XT beads (IBA LifeSciences, Germany), and flag-tagged SARS-CoV-2 ORFs were immunoprecipitated using Anti-DYKDDDDK Magnetic Agarose (Pierce ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was filtered through a 5.0 µm syringe filter followed by 0.45 µm syringe filters and loaded on to 3 mL gravity flow column packed with StrepTactin Sepharose resin (IBA Lifesciences). The column was washed with wash buffer (20 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2022Quote: ... FLAG beads were rinsed with the same buffer for an additional elution which was combined with the initial peptide elution and incubated with MagStrep “type 3” magnetic bead suspension (IBA Lifesciences) for 2h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... coli MG1655 genomic DNA with primers 5’ CTC GGT CTC CAA TGA ATA AAT ATC AGG CAG TGA 3’ and 5’ CTC GGT CTC AGC GCT TTA TTT GAC TAA TGA AAA TAG ATC A 3’ and cloned into the Eco31I (BsaI) sites of plasmid pPR-IBA101 (IBA Lifesciences) to yield plasmid pRCLA10 ...
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bioRxiv - Neuroscience 2021Quote: ... human SARM1 without mitochondria targeting sequence and with N-terminal tandem StrepTag II was expressed in HEK293T cells expressing NRK1 supplemented with NR (Essuman et al., 2017) and purified with MagStrep (Strep-Tactin) type 3 XT beads suspension (IBA Lifesciences). The purified protein attached on beads was incubated with NAD+ (10 μM for Figure 5 B ...
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bioRxiv - Cell Biology 2019Quote: The custom 20-mer oligonucleotide modified with dibenzocyclooctyne (DBCO) (Sequence: 5’ - TCA GTT CAG GAC CCT CGG CT – DBCO – 3’) was purchased from IBA Life Sciences, reconstituted in nuclease free water at a concentration of 600 μM and stored at −20°C ...
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bioRxiv - Cell Biology 2022Quote: ... StrepMAB-Classic (IBA LifeSciences, WB 1:2000, IF 1:500), StrepMAB-Classic DY-549 (IBA LifeSciences ...
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bioRxiv - Systems Biology 2023Quote: ... 1 mM DTT) and 50 μl 1× buffer BXT (IBA Lifesciences) and dispensed into a single 96-well KingFisher microtitre plate ...
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bioRxiv - Microbiology 2021Quote: ... 50 µl of a 10% slurry of Strep-Tactin Resin (IBA Lifesciences #2-1201-002), which was pre-equilibrated in binding buffer ...
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bioRxiv - Biochemistry 2023Quote: ... The bound proteins were eluted with the binding buffer supplemented with 10 mM desthiobiotin (IBA Lifesciences). The samples were analyzed by SDS-PAGE and detected by immunoblotting using antibodies to the strep tag (1:3,000 ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound proteins were eluted with the binding buffer supplemented with 10 mM desthiobiotin (IBA Lifesciences). The samples were analyzed by SDS-PAGE and detected by Coomassie staining.
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bioRxiv - Biochemistry 2023Quote: ... After equilibration of the Strep-tactin XT Superflow resin (IBA lifesciences, 10 mL per liter of cell supernatant) in 0.1 M Tris-HCl pH 8 ...
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bioRxiv - Biochemistry 2021Quote: ... pH 7.5) in a PD-10 column before rocking with 500μL of Strep-T actin Sepharose resin slurry (IBA Lifesciences) for 45 min ...
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bioRxiv - Genetics 2023Quote: ... 1 ml StrepTactin (IBA Lifesciences, Göttingen, Germany) pre-equilibrated with lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... Strep-Tactin-DY649 (IBA LifeSciences, IF 1:2000), mouse anti-CIMPR (2G11 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) containing 2.5 mM desthiobiotin (IBA Lifesciences). Eluted protein was concentrated and run on a Superose 6 10/300 GL size exclusion column (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mL of BioLock biotin blocking solution (IBA Lifesciences) were added to 1.5 L conditioned medium ...
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bioRxiv - Biochemistry 2022Quote: ... and StrepMAB classic DY649-conjugate (1:250, IBA Lifesciences) for the unstained samples ...
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bioRxiv - Biochemistry 2022Quote: ... and StrepMAB classic DY649-conjugate (1:150, IBA Lifesciences) for the unstained samples ...
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bioRxiv - Cell Biology 2022Quote: ... StrepMAB-Classic DY-549 (IBA LifeSciences, FC 1:500), Strep-Tactin-DY649 (IBA LifeSciences ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were incubated for 1 h at room temperature in blocking solution with mouse StrepMAB antibody (IBA Lifesciences, 1:750, 2-1507-001) for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8 ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with 1 ml Strep-Tactin resin (IBA Lifesciences) for 2 hours at 4°C with rotation ...