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Citations for IBA Life Sciences :
51 - 100 of 108 citations for 6 tert Butyl 4 chlorothieno 2 3 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Eluted fractions were combined and incubated for 1 h at 4°C with Strep-Tactin agarose beads (IBA Lifesciences). Beads were applied to a gravity column ...
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bioRxiv - Microbiology 2021Quote: ... 50 µl of a 10% slurry of Strep-Tactin Resin (IBA Lifesciences #2-1201-002), which was pre-equilibrated in binding buffer ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was mixed with 2 mL Strep-Tactin XT resin (IBA Lifesciences, Göttingen, Germany). The resin was washed extensively with wash buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was mixed with 2 ml Strep-Tactin XT resin (IBA Lifesciences, Göttingen, Germany). The resin was washed with wash buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... or Strep-Tactin®XT 4Flow high capacity resin (IBA life sciences, 2-5030-002). Bound proteins were eluted with 0.2 mg/mL 1xFLAG (DYKDDDK) ...
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bioRxiv - Molecular Biology 2022Quote: ... SARS-COV-2 NTD proteins were purified with Sepharose Strep-Tactin beads (IBA Life Sciences) as previously described [75].
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was incubated with 2ml Strep-Tactin resin (#2-1201-025, IBA Lifesciences) for 30 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... A 1 ml Strep-Tactin XT 4Flow high-capacity cartridge (IBA Lifesciences 2-5027-001) was washed with 14 column volumes of wash buffer (50 mM Hepes pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... and the supernatant was incubated with 2ml Strep-Tactin resin (#2−1201-025, IBA Lifesciences) for 30 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and recombinant proteins were purified using Strep-Tactin XT resin (IBA Lifesciences, Cat. #2-5010), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore ...
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bioRxiv - Plant Biology 2020Quote: ... The resin was then washed 3 times with RIPA buffer and eluted with 1’ Strep-Tactin elution buffer with biotin (IBA Life Sciences). The input ...
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bioRxiv - Cell Biology 2019Quote: The custom 20-mer oligonucleotide modified with dibenzocyclooctyne (DBCO) (Sequence: 5’ - TCA GTT CAG GAC CCT CGG CT – DBCO – 3’) was purchased from IBA Life Sciences, reconstituted in nuclease free water at a concentration of 600 μM and stored at −20°C ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM TCEP) with pre-equilibrated magnetic Strep-Tactin XT beads (240 µl MagStrep type 3 XT bead slurry, IBA life sciences). Following 1 h of binding at room temperature on a rotator ...
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bioRxiv - Biochemistry 2023Quote: ... lysates were clarified by centrifugation (17,000 x rpm for 40 min at 4 °C) and incubated with Strep-Tactin Sepharose (IBA Lifesciences) at 4 °C overnight ...
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bioRxiv - Cancer Biology 2023Quote: ... The extract was incubated with Strep-Tactin XT superfluid resin (2-4010; IBA Lifesciences, Gottingen, Germany) at 4°C for 4 hours ...
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bioRxiv - Biophysics 2019Quote: ... The lysate was centrifuged (30 min, 60,000xg, 4°C) and the supernatant was incubated with strep-tactin resin (Strep-Tactin® resin, Iba Lifesciences) for 30min at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... which were incubated for 30 min at 4°C with 50 ul of ferrimagnetic StrepTactin beads (MagStrep Type3 XT beads, IBA LifeSciences) preincubated in 0.1M Tris pH8.0 ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was centrifuged at 30,000 x g for 40 min at 4 °C in a JA 20 rotor and the supernatant was mixed with Strep-Tactin resin (IBA Lifesciences) previously equilibrated with buffer A ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20 μL Strep-Tactin Macroprep beads (IBA lifesciences). Beads were washed three times with lysis buffer ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: nProtein A Sepharose 4 Fast Flow resin (Cytiva) was used for affinity purification of adalimumab whilst Strep-Tactin XT 4Flow resin (IBA Lifesciences) was used for Strep-II tagged-tagged proteins ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20-30 μl Strep-Tactin Macroprep beads (IBA lifesciences), FLAG or Ni-NTA beads (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation at 50,000 g for 30 minutes at 4°C and applied to pre-equilibrated StrepTactin resin (IBA Life Sciences). The soluble extract was incubated with the resin for one hour at 4°C with constant rotation ...
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bioRxiv - Biophysics 2023Quote: ... Supernatants were incubated with end-over-end rotation for 1 hour at 4°C with Strep-Tactin Superflow high-capacity resin (IBA Lifesciences) equilibrated with gel-filtration buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: ... Clarified lysate was placed on a Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in AcrIIA11 Lysis Buffer ...
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bioRxiv - Biochemistry 2021Quote: Labelled and unlabelled DNA oligos to make lacCONS+2 promoter constructs were purchased from IBA Lifesciences (Germany) (Fig ...
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bioRxiv - Genetics 2021Quote: ... Strep-tagged Orf8 complexes were eluted from beads in Buffer BXT (IBA Lifesciences; Cat. # 2-1042-025) shaking at 1100 rpm for 30 min.
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bioRxiv - Genetics 2021Quote: ... Strep-tagged Orf8 complexes were eluted from beads in Buffer BXT (IBA Lifesciences; Cat. # 2-1042-025) shaking at 1100 rpm for 30 min.
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bioRxiv - Microbiology 2022Quote: ... 2xStrep tagged S-protein was stained using StrepMAB-Classic DY-488 conjugate (IBA Lifesciences, 2-1563-050) and FLAG tagged S-protein was stained using anti-FLAG (clone M2 ...
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bioRxiv - Biochemistry 2024Quote: ... StrepTactin XT beads (2-5010-025) and biotin (0-1016-002) were purchased from IBA Lifesciences (Germany). Dihydroartemisinin (DHA ...
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bioRxiv - Microbiology 2024Quote: ... 1mM EDTA and 2 mM biotin) and eluted with 3X volume of BXT buffer (IBA Life Sciences). Samples were run on 12% TGX gels (Biorad ...
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bioRxiv - Biochemistry 2023Quote: ... Then the lysates were centrifuged at 15,000 x g for 20 min at 4°C and the supernatant was passed over a column packed with Strep-tactin XT 4Flow resin (Iba Lifesciences GmbH). The column was washed with lysis buffer ...
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bioRxiv - Systems Biology 2021Quote: ... The supernatant was passed through a column packed with Strep-Tactin® resin (IBA LifeSciences #2-1208-010) and washed with IBA LifeSciences Buffer W (NC0612462 ...
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bioRxiv - Immunology 2021Quote: ... before staining cells with DY-549-labeled strep-tactin (1:100 dilution, IBA lifesciences, Cat #2-1565-050) or Allphycocyanin-labeled streptavidin (1:200 dilution ...
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bioRxiv - Cancer Biology 2019Quote: ... pooled and loaded onto a gravity column containing Strep-Tactin Superflow high capacity resin (#2-1208-025, Iba Lifesciences). Strep-tagged proteins were eluted using 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV S2P was further purified using a 2ml Strep-Tactin sepharose column (IBA Lifesciences Cat# 2-1201-002) and Strep-Tactin Purification Buffer Set (IBA Lifesciences Cat # 2-1002-001 ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated with StrepMAB-Classic monoclonal antibody conjugated to HRP (0.05 µg/mL, #2-1509-001, Iba Lifesciences) for 2 h at RT ...
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bioRxiv - Microbiology 2024Quote: ... Cell debris was removed by centrifugation at 30,000xg and 4°C for 45 min and the resulting crude extract was applied onto a Strep-Tactin®XT 4Flow® column (IBA Lifesciences, Germany) and purified following the manufacturers’ instructions ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: StrepMAB-Immo (anti-Twin-Strep-tag®; IBA Lifesciences; 2-1517-001; 1:4000), FUS (1:2000 ...
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bioRxiv - Immunology 2021Quote: Purified E1E2 + AR4A-StrepII complexes (1.0 μg ml™1 in TBS with 0.1% DDM) were coated for 2 h at room temperature on 96-well Strep-Tactin XT coated microplates (IBA LifeSciences). Plates were washed twice with TBS/0.05% Tween20 before incubating with serially diluted mAbs and CD81-LEL-hFc (R&D systems ...
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bioRxiv - Microbiology 2023Quote: ... Concentrated proteins were resuspended in buffer W at pH 8 and incubated with BioLock (#2-0205-050, Iba Life sciences) for 15 min at RT to neutralize biotin ...
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bioRxiv - Immunology 2024Quote: ... stabilized histidine-tagged SARS-CoV-2 S protein (Wuhan, XBB.1.5, BA.2.86) was labelled with Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and conjugated to magnetic beads according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble membrane fraction was diluted 2-fold with buffer B and applied to a column packed with Strep-Tactin beads (IBA Lifesciences) by gravity flow (6–10 s/drop ...
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bioRxiv - Microbiology 2020Quote: Purified StrepII-tagged sE2 monomer (1.0 µg/mL in TBS) was coated for 2 hour at room temperature on 96-well Strep-TactinXT coated microplates (IBA LifeSciences). Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems ...
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bioRxiv - Plant Biology 2020Quote: ... They were next incubated in primary antibody solution for 2 hours at room temperature (1:1000 anti-Strep, IBA Life Sciences, 1:10,000 anti-FLAG M2 monoclonal antibody ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gravity flow columns (2 mL) were prepared with 400 μL 50% Strep-Tactin® XT Superflow High Capacity suspension (IBA Lifesciences). Protein purification was performed according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... Cellular debris was pelleted by ultracentrifugation before placing lysate over a Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Insoluble materials were discarded and clear lysates were added to Strep-Tactin sepharose (400 μL 50% slurry; IBA Lifesciences #2-1201-010) loaded columns (Bio-Rad Laboratories #732-6008 ...
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bioRxiv - Molecular Biology 2023Quote: ... we added an additional purification step and incubated the Flag-eluted samples with Strep-TactinXT 4Flow beads (IBA lifesciences, 2-5030-010) for 2 hours ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was washed five times with IP buffer and eluted with Strep-Tactin elution buffer (IBA Life Sciences, 2-1000-025). Eluted samples were analyzed by western blot.
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bioRxiv - Microbiology 2024Quote: ... 0.5-1 mg of lysate was incubated overnight with 30 ul of Strep-Tactin Sepharose resin (IBA Life Science, 2-1201-002), rotating at 4°C ...