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Citations for IBA Life Sciences :
1 - 50 of 106 citations for 6 oxo 1 phenyl 1 4 5 6 tetrahydro pyridazine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Transfected cells were sorted for HDR+ by staining for Strep-Tactin-APC 1:100 (IBA lifesciences, Cat: 6-5010-001) and anti-hIgG AF488 1:100 (Jackson ImmunoResearch ...
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bioRxiv - Biochemistry 2021Quote: ... were formed as described previously (Sidorenkov et al., 1998) using scaffold 1 (Table 6) and the RNA sequence: AUCGAGAGG (IBA life sciences). Briefly ...
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bioRxiv - Biophysics 2022Quote: ... before being loaded into a 6 mL Strep-Tactin Superflow high capacity resin (IBA Lifesciences, USA). The resin was washed using 6 column volumes of Buffer W1 (20 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2021Quote: ... hnRNPH1: 5′-CUCUUGUCCAUCUAGAC-3′] used for the NMR titration was purchased (IBA Lifesciences).
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bioRxiv - Plant Biology 2023Quote: ... The elution fractions containing the trxA-14-3-3 or trxA-BIN2 fusion proteins were loaded onto a 5 ml Strep-Tactin Superflow High Capacity column (IBA Lifesciences), washed with buffer A and eluted in buffer A supplemented with 2.5 mM desthiobiotin ...
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bioRxiv - Microbiology 2022Quote: ... Cell culture supernatant was harvested 6 days after transfection and purified using Strep-Tactin XT Superflow high capacity resin (IBA Lifesciences) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: ... The column was washed with 5 volumes of equilibration buffer and eluted with 6 volumes of the same buffer supplemented with 50 mM D-biotin (IBA lifesciences). The fractions containing Mini-I were pooled and concentrated by ultrafiltration (Vivaspin 20 ...
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bioRxiv - Molecular Biology 2020Quote: ... The elute was then incubated for 1 h at 4 °C with Strep-Tactin beads (IBA Lifesciences) before application to a gravity column ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM EDTA) and loaded onto a 5 ml Strep-Tactin Superflow High Capacity colum (IBA Lifesciences). Proteins were eluted from the column in Strep buffer supplement with 2.5 mM desthiobiotin followed by over-night TEV cleavage of the N-terminal affinity tag for 16 h at 4 °C during dialysis against Strep buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted fractions were combined and incubated for 1 h at 4°C with Strep-Tactin agarose beads (IBA Lifesciences). Beads were applied to a gravity column ...
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bioRxiv - Molecular Biology 2023Quote: ... The complex was eluted in five 1 mL fractions using buffer C with added 5 mM desthiobiotin (IBA Lifesciences). After addition of TCEP to 1 mM ...
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bioRxiv - Plant Biology 2020Quote: ... The resin was then washed 3 times with RIPA buffer and eluted with 1’ Strep-Tactin elution buffer with biotin (IBA Life Sciences). The input ...
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bioRxiv - Cell Biology 2023Quote: ... 1 mM TCEP) with pre-equilibrated magnetic Strep-Tactin XT beads (240 µl MagStrep type 3 XT bead slurry, IBA life sciences). Following 1 h of binding at room temperature on a rotator ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20 μL Strep-Tactin Macroprep beads (IBA lifesciences). Beads were washed three times with lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20-30 μl Strep-Tactin Macroprep beads (IBA lifesciences), FLAG or Ni-NTA beads (Sigma) ...
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bioRxiv - Biophysics 2023Quote: ... Supernatants were incubated with end-over-end rotation for 1 hour at 4°C with Strep-Tactin Superflow high-capacity resin (IBA Lifesciences) equilibrated with gel-filtration buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated either with anti-strep tag antibody (IBA Lifesciences) or anti-SARS-COV-2 polyclone antibody (Sino Biological Inc. ...
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bioRxiv - Cell Biology 2022Quote: ... StrepMAB-Classic (IBA LifeSciences, WB 1:2000, IF 1:500), StrepMAB-Classic DY-549 (IBA LifeSciences ...
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bioRxiv - Immunology 2022Quote: ... in TSM binding buffer at 4°C for 1 h before StrepMAB-Classic Oyster 645 conjugate (0.5 μg/mL, IBA Lifesciences 2-1555-050) was added ...
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bioRxiv - Systems Biology 2023Quote: ... 1 mM DTT) and 50 μl 1× buffer BXT (IBA Lifesciences) and dispensed into a single 96-well KingFisher microtitre plate ...
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bioRxiv - Cell Biology 2019Quote: The custom 20-mer oligonucleotide modified with dibenzocyclooctyne (DBCO) (Sequence: 5’ - TCA GTT CAG GAC CCT CGG CT – DBCO – 3’) was purchased from IBA Life Sciences, reconstituted in nuclease free water at a concentration of 600 μM and stored at −20°C ...
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bioRxiv - Genetics 2023Quote: ... 1 ml StrepTactin (IBA Lifesciences, Göttingen, Germany) pre-equilibrated with lysis buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... 5% suspension (IBA Lifesciences) and eluted with desthiobiotin ...
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bioRxiv - Cell Biology 2022Quote: ... Strep-Tactin-DY649 (IBA LifeSciences, IF 1:2000), mouse anti-CIMPR (2G11 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mM DTT) containing 2.5 mM desthiobiotin (IBA Lifesciences). Eluted protein was concentrated and run on a Superose 6 10/300 GL size exclusion column (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... 1 mL of BioLock biotin blocking solution (IBA Lifesciences) were added to 1.5 L conditioned medium ...
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bioRxiv - Biochemistry 2022Quote: ... and StrepMAB classic DY649-conjugate (1:250, IBA Lifesciences) for the unstained samples ...
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bioRxiv - Biochemistry 2022Quote: ... and StrepMAB classic DY649-conjugate (1:150, IBA Lifesciences) for the unstained samples ...
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bioRxiv - Cell Biology 2022Quote: ... StrepMAB-Classic DY-549 (IBA LifeSciences, FC 1:500), Strep-Tactin-DY649 (IBA LifeSciences ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were incubated for 1 h at room temperature in blocking solution with mouse StrepMAB antibody (IBA Lifesciences, 1:750, 2-1507-001) for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8 ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with 1 ml Strep-Tactin resin (IBA Lifesciences) for 2 hours at 4°C with rotation ...
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bioRxiv - Neuroscience 2021Quote: ... Twin-Strep-tag® (IBA Lifesciences; 2-1517-001; 1:1000); and Streptavidin 660 Conjugate (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... using α-strep-tag mouse antibodies 1:3000 (IBA Life Sciences). Subsequently ...
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bioRxiv - Systems Biology 2020Quote: ... 1 mM DTT) and 50 μl 1x Buffer BXT (IBA Lifesciences) dispensed into a single 96-well KF microtiter plate ...
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bioRxiv - Genetics 2021Quote: ... streptactin DY-488 (IBA Lifesciences; Cat. # 2-1562-050; 1:500) was added to secondary antibody solution ...
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bioRxiv - Microbiology 2022Quote: ... StrepMAB-Classic (1:500, mouse monoclonal, IBA lifesciences, 2-1507-001), anti-SARS-CoV-2 Spike (1:100 ...
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bioRxiv - Biochemistry 2019Quote: The BBS2-7-9 complex was purified at 4°C by affinity purification using 4 mL of Strep-Tactin resin (IBA Life Sciences) packed in a 2 cm diameter column and equilibrated with equilibration buffer (20 mM HEPES pH 7.5 and 20 mM NaCl) ...
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bioRxiv - Biochemistry 2023Quote: 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h gently stirring at 4°C ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was incubated with 1 mL Streptactin sepharose beads (IBA Life Sciences) for 1 h ...
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bioRxiv - Synthetic Biology 2019Quote: ... coli cells (5-1600-020, IBA Life Sciences, Göttingen). Transformants were screened by colony PCR and verified by sequencing (StabVida ...
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was loaded on a 1-mL Strep-Tactin column (IBA Lifesciences). Purification was performed as specified by the manufacturer ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity chromatography was performed with Strep-Tactin Sepharose slurry (1 mL/L) (IBA Lifesciences), using 1xTris buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2023Quote: ... from 0 mM to 5 mM desthiobiotin (IBA Lifesciences GmbH), was then used to elute each complex ...
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bioRxiv - Biochemistry 2023Quote: ... A 1 ml Strep-Tactin XT 4Flow high-capacity cartridge (IBA Lifesciences 2-5027-001) was washed with 14 column volumes of wash buffer (50 mM Hepes pH 7.6 ...
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was performed with MagStrep type 3 XT beads (Iba Life Sciences, Gottingen, Germany) according to the manufacturers protocol ...
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bioRxiv - Biochemistry 2019Quote: ... 1 mM EDTA) and the kinase was eluted in 1x Biotin elution buffer BXT (IBA lifesciences). Plk1 was isolated from bacteria ...
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bioRxiv - Plant Biology 2023Quote: ... and supplemented with 75 μl·ml-1 Strep-Tactin Macroprep (50% slurry) (IBA Lifesciences GmbH, Göttingen, Germany). Samples were incubated in a rotary wheel at 4°C for one hour ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was added to magnetic Strep beads (MagStrep type 3 beads, IBA Life science) previously equilibrated with buffer A and incubated for 30 min at 4 °C on a roller ...
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bioRxiv - Cancer Biology 2022Quote: ... Washed magnetic Streptactin beads (100 μl, MagStrep type 3 XT, IBA Life Sciences, Gottingen, Germany) were added and incubated overnight at 4°C with agitation ...
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bioRxiv - Molecular Biology 2023Quote: ... The supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4°C ...