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Citations for IBA Life Sciences :
251 - 267 of 267 citations for WAS Protein Family Member 3 WASF3 Antibody Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2019Quote: ... DNA labeled at two locations for ensemble FRET experiments was prepared by large scale PCR of two DNA templates using labeled primers (IBA Life Sciences). 120μg of each DNA template was digested with AflIII at 37°C overnight and purified by native PAGE ...
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bioRxiv - Biochemistry 2023Quote: ... Then the lysates were centrifuged at 15,000 x g for 20 min at 4°C and the supernatant was passed over a column packed with Strep-tactin XT 4Flow resin (Iba Lifesciences GmbH). The column was washed with lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... The insoluble material was removed by centrifugation (12,500g, 15 min) and the supernatant was incubated with 300 mL Strep-Tactin® Superflow® resin (IBA lifesciences) while rotating slowly for 2h at 4°C ...
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bioRxiv - Biophysics 2023Quote: The gene encoding human THSD7A domains d1_d2 (UNIPROT Q9UPZ6, residues A48–Q192) was sub-cloned into the eukaryotic expression vector pDSG-IBA104 (IBA Lifesciences, Göttingen, Germany). It positioned a BM40 secretion signal ...
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bioRxiv - Biochemistry 2024Quote: ... A pulldown was performed by incubating the RNA/ZnF mixture with 1 μl of washed MagStrep “type3” XT beads (IBA Lifesciences, Göttingen) for 1 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 0.5-1 mg of lysate was incubated overnight with 30 ul of Strep-Tactin Sepharose resin (IBA Life Science, 2-1201-002), rotating at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... The cleared lysate was loaded onto a gravity-flow column with Strep-Tactin® Superflow® high capacity resin (IBA Lifesciences GmbH) pre-equilibrated with Buffer W ...
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bioRxiv - Biochemistry 2024Quote: ... A pulldown was performed by incubating the RNA/ARID mixture with 1 μl of washed MagStrep “type3” XT beads (IBA Lifesciences, Göttingen) for 1 h at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... The Streptag pulldown was completed in the same manner using 100 μL of MagStrep "type3" XT bead suspension (IBA Lifesciences, 2-4090-002), except elution was achieved by boiling with sample buffer at 95 °C for 2 minutes.
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bioRxiv - Biophysics 2020Quote: ... and a PAGE-purified primer with an internal Cy5 for generating the 60/60 construct was purchased from IBA Life Sciences (Gottingen, Germany). All nucleosomes were assembled on the 601 nucleosome positioning sequence ([44]).
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bioRxiv - Biochemistry 2023Quote: The library encoding the partly randomised de novo designed gene S-824 was cloned from the original pET11a vector16 into the high-copy number vector pASK-IBA5+ (IBA Life Sciences, Germany) using the restriction sites XbaI and BamHI ...
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bioRxiv - Biochemistry 2024Quote: ... The complex was purified by pulling down from the Strep-tag in AtRPAP3 with a StrepTactin®XT 4Flow® column (IBA Lifesciences) using the same protocol described for AtRPAP3 ...
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bioRxiv - Cell Biology 2024Quote: ... TwinStrep-tagged: The lysate was passed through chromatography columns containing 1.5 ml bed volume of Strep-Tactin® Sepharose® resin (IBA Lifesciences, Germany). Resin was washed with ∼20 resin volumes of 100 mM HEPES ...
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bioRxiv - Biophysics 2019Quote: ... the C-terminal His tag was cleaved by treatment with TEV protease and passed over the Ni-NTA and Strep-Tactin XT Superflow (IBA Life Sciences, Göttingen, Germany) columns attached in tandem ...
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bioRxiv - Biophysics 2021Quote: ... The C-terminal His-tag was then cleaved by treatment with TEV protease and applied to Ni-NTA and Strep-Tactin XT Superflow column (IBA life Sciences, Göttingen, Germany) attached in tandem and washed ...
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bioRxiv - Microbiology 2024Quote: ... Cell debris was removed by centrifugation at 30,000xg and 4°C for 45 min and the resulting crude extract was applied onto a Strep-Tactin®XT 4Flow® column (IBA Lifesciences, Germany) and purified following the manufacturers’ instructions ...
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bioRxiv - Biophysics 2021Quote: ... The TS-SUMO-SaCas9-sgRNA or TS-SUMO-SaCas9 cell lysate was applied to a 5 mL Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer containing 200 mM NaCl ...