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Citations for IBA Life Sciences :
151 - 200 of 295 citations for Recombinant Human FCGRT & B2M Protein His Avi Strep II tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and the supernatant was loaded on a 1-mL Strep-Tactin column (IBA Lifesciences). Purification was performed as specified by the manufacturer ...
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bioRxiv - Biochemistry 2021Quote: ... The membrane was subsequently incubated with an anti-Strep antibody (StrepMAB-Classic, IBA Lifesciences) at 4°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... Secreted nCDase was purified from culture medium using Strep-Tactin resin (IBA Life Sciences) and further purified using a Superdex 200 size-exclusion chromatography column equilibrated in nCDase reaction buffer (10 mM HEPES ...
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bioRxiv - Immunology 2022Quote: ... The digest was rotated with 200 μL of Strep-Tactin Superflow resin (IBA Lifesciences) at room temperature (RT ...
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bioRxiv - Biochemistry 2023Quote: ... Affinity chromatography was performed with Strep-Tactin Sepharose slurry (1 mL/L) (IBA Lifesciences), using 1xTris buffer (25 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was added to Strep-Tactin®XT 4Flow®resin (IBA Life Sciences) and incubated for 1 hour at 4°C with rocking ...
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bioRxiv - Microbiology 2024Quote: ... Supernatant was incubated with Strep-Tactin®XT 4Flow®resin (IBA Life Sciences) resin for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... GFP-RadA was eluted with Strep Buffer complemented with 2.5 mM desthiobiotin (IBA Lifesciences). The fusion protein was cleaved with ∼ 80 µg/mL TEV protease (home-made ...
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bioRxiv - Cell Biology 2024Quote: ... The Twin-Strep-tag® capture kit was from Iba Lifesciences (IBA GmbH, Germany). Peptides were purchased from Peptide 2.0 Inc (Chantilly ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was added to magnetic Strep beads (MagStrep type 3 beads, IBA Life science) previously equilibrated with buffer A and incubated for 30 min at 4 °C on a roller ...
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bioRxiv - Plant Biology 2020Quote: ... and the supernatant loaded onto a column containing Strep-Tactin XT resin (IBA Life Sciences) and protein was purified according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2021Quote: ... 50 µl of a 10% slurry of Strep-Tactin Resin (IBA Lifesciences #2-1201-002), which was pre-equilibrated in binding buffer ...
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bioRxiv - Microbiology 2021Quote: ... Western blots were probed with anti-Strep and anti-PhoA antisera (IBA Lifesciences, Sigma-Aldrich) (Scheu et al ...
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bioRxiv - Microbiology 2021Quote: ... The following primary antibodies were used in this study: Strep-Tactin-HRP conjugate (Iba Lifesciences) (dilution 1:3,000 in 3 w/v % BSA/TBS-T) ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was mixed with 2 mL Strep-Tactin XT resin (IBA Lifesciences, Göttingen, Germany). The resin was washed extensively with wash buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2020Quote: ... after which the complex was passed over Strep-Tactin XT resin (IBA Lifesciences—Goettingen, Germany). Bound complex was eluted using Buffer BXT containing 50 mM biotin (IBA Lifesciences) ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was mixed with 2 ml Strep-Tactin XT resin (IBA Lifesciences, Göttingen, Germany). The resin was washed with wash buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... TBS) were added to 96-well Strep-TactinXT-coated plates (IBA Lifesciences, Göt-tingen, Germany) for 2 hours at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... and applied to Strep-Tactin affinity chromatography according to the manufacturer’s protocol (IBA Life Sciences). The protein was eluted with buffer D containing 5 mM d-Desthiobiotin ...
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bioRxiv - Molecular Biology 2022Quote: ... or Strep-Tactin®XT 4Flow high capacity resin (IBA life sciences, 2-5030-002). Bound proteins were eluted with 0.2 mg/mL 1xFLAG (DYKDDDK) ...
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bioRxiv - Cell Biology 2023Quote: ... the lysates cleared by centrifugation were incubated with Strep-Tactin® Sepharose resin (IBA Lifesciences). Purified proteins were then eluted by desthiobiotin using Buffer E (IBA Lifesciences ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was incubated with 2ml Strep-Tactin resin (#2-1201-025, IBA Lifesciences) for 30 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 500 µL of supernatant was applied to 20 Strep-Tactin®XT resin (IBA Lifesciences); for MBP pull-downs ...
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bioRxiv - Biochemistry 2023Quote: ... A 1 ml Strep-Tactin XT 4Flow high-capacity cartridge (IBA Lifesciences 2-5027-001) was washed with 14 column volumes of wash buffer (50 mM Hepes pH 7.6 ...
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bioRxiv - Biochemistry 2023Quote: ... then loaded onto a column packed with strep-tactin XT 4Flow resin (Iba Lifesciences GmbH). The column was washed with 5 column volume of lysis buffer containing 250 mM NaCl followed by 10 column volume of lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... and the supernatant was incubated with 2ml Strep-Tactin resin (#2−1201-025, IBA Lifesciences) for 30 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... all from Novopep Limited) were loaded onto Strep-Tactin Sepharose beads (50% suspension; IBA Lifesciences) or streptavidin agarose beads (50% suspension ...
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bioRxiv - Microbiology 2023Quote: ... The following primary antibodies were used in this study: Strep-Tactin-HRP conjugate (Iba Lifesciences) (dilution 1:3,000 in 3 w/v % BSA/TBS-T) ...
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bioRxiv - Microbiology 2020Quote: ... and purified from supernatant with a Gravity Flow Strep-Tactin Superflow high capacity column (IBA Lifesciences). Purified CD81-LEL was polished by size exclusion chromatography (SEC ...
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bioRxiv - Developmental Biology 2022Quote: ... the cell culture supernatants were applied to a Strep-tactin column (IBA Lifesciences GmbH, Göttingen, Germany) and eluted with 2.5 mM desthiobiotin ...
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bioRxiv - Molecular Biology 2020Quote: ... The clarified supernatant was purified in two steps: via a Strep-Tactin XT column (IBA Lifesciences) followed by Superose 6 10/300 GL column (GE Healthcare ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... before being incubated with 25 µl of Strep-Tactin®XT agarose beads (IBA Lifesciences, Germany) overnight at 4 °C in presence of protease inhibitor cocktail (Roche ...
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bioRxiv - Cancer Biology 2023Quote: ... The extract was incubated with Strep-Tactin XT superfluid resin (2-4010; IBA Lifesciences, Gottingen, Germany) at 4°C for 4 hours ...
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bioRxiv - Biophysics 2022Quote: ... before being loaded into a 6 mL Strep-Tactin Superflow high capacity resin (IBA Lifesciences, USA). The resin was washed using 6 column volumes of Buffer W1 (20 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2023Quote: ... and supplemented with 75 μl·ml-1 Strep-Tactin Macroprep (50% slurry) (IBA Lifesciences GmbH, Göttingen, Germany). Samples were incubated in a rotary wheel at 4°C for one hour ...
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bioRxiv - Biochemistry 2023Quote: ... or 2 mL of Strep-Tactin® Sepharose® resin (IBA Lifesciences GmbH, #2-1201-002) for strep-II tagged proteins ...
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bioRxiv - Developmental Biology 2024Quote: ... the cell lysate was loaded onto preequilibrated Strep-Tactin XT 4Flow resin (IBA Lifesciences GmbH, Germany) and washed with wash buffer (500 mM NaCl ...
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bioRxiv - Microbiology 2024Quote: ... Cell debris were pelleted and clear lysate was loaded on Strep-Tactin® resin (IBA Lifesciences) pre-equilibrated with Strep Buffer (50 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2021Quote: ... Clarified lysate was placed on a Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in AcrIIA11 Lysis Buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... The elute was then incubated for 1 h at 4 °C with Strep-Tactin beads (IBA Lifesciences) before application to a gravity column ...
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bioRxiv - Cell Biology 2020Quote: ... and the MBP-LaNt α31 purified using Strep-Tactin® sepharose 50% suspension (IBA Lifesciences, Göttingen, Germany), eluted with 1.875 mg ml-1 desthiobiotin in TBS ...
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bioRxiv - Biochemistry 2022Quote: ... A CM5 sensor chip (Cytiva) was prepared by EDC/NHS coupling of Strep-Tactin-XT (IBA Lifesciences). Running buffer was 8mM TRIS ...
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bioRxiv - Physiology 2022Quote: ... the thawed medium was loaded onto Strep-Tactin® Superflow® high capacity cartridge (IBA Lifesciences GmbH) pre-equilibrated in the binding buffer (100 mM Tris-HCl pH 8.0 ...
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bioRxiv - Biochemistry 2022Quote: ... The PEI supernatant was then loaded onto a Strep-Tactin XT 4F HC resin (IBA Life Sciences) at a flow rate of 1 mL/min (approximately 1 mL bed volume per 100 mg total protein ...
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bioRxiv - Biochemistry 2022Quote: ... The PEI supernatant was then loaded onto a Strep-Tactin XT 4F HC resin (IBA Life Sciences) at a flow rate of 1 mL/min (approximately 1 mL bed volume per 100 mg total protein ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The cleared extract was passed through 1 mL Strep - Tactin XT Superflow (IBA Lifesciences GmbH, Göttingen, Germany) column pre-equilibrated with 250C buffer (25 mM HEPES - KOH pH 7.6 ...
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bioRxiv - Plant Biology 2023Quote: ... 1 mM EDTA) and loaded onto a 5 ml Strep-Tactin Superflow High Capacity colum (IBA Lifesciences). Proteins were eluted from the column in Strep buffer supplement with 2.5 mM desthiobiotin followed by over-night TEV cleavage of the N-terminal affinity tag for 16 h at 4 °C during dialysis against Strep buffer ...
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bioRxiv - Cell Biology 2023Quote: ... The soluble supernatant was collected and incubated with pre-equilibrated Strep-Tactin Sepharose beads (IBA Life Sciences) for binding of the Twin-Strep-tagged ULK1 protein ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were floated in a solution of 1:50000 Strep-Tactin HRP conjugate (IBA Lifesciences, Germany) with 0.1% (w/v ...
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bioRxiv - Cell Biology 2024Quote: ... then the soluble lysate was incubated with 1mL equilibrated Strep-Tactin® Sepharose® resin (IBA Lifesciences) rotating for 1 hour at 4°C ...