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Citations for IBA Life Sciences :
51 - 89 of 89 citations for Streptavidin magnetic beads since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 1mM DTT) and 30 µl of washed Strep-Tactin Superflow beads (Iba LifeSciences) were added and rotated for 2 hours at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Immunoprecipitation was performed with MagStrep type 3 XT beads (Iba Life Sciences, Gottingen, Germany) according to the manufacturers protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... complexes bound to beads were washed and then eluted with desthiobiotin (IBA Life Sciences). Proteins from cell lysates and AP eluates were evaluated by MS as below or separated by SDS-PAGE and either directly stained using the Pierce Silver Stain Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... SARS-COV-2 NTD proteins were purified with Sepharose Strep-Tactin beads (IBA Life Sciences) as previously described [75].
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bioRxiv - Cell Biology 2023Quote: ... all from Novopep Limited) were loaded onto Strep-Tactin Sepharose beads (50% suspension; IBA Lifesciences) or streptavidin agarose beads (50% suspension ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4°C ...
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bioRxiv - Genomics 2020Quote: ... 800 μL supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4ºC ...
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Maize AFP1 confers antifungal activity by inhibiting chitin deacetylases from a broad range of fungibioRxiv - Microbiology 2021Quote: ... before being incubated with 25 µl of Strep-Tactin®XT agarose beads (IBA Lifesciences, Germany) overnight at 4 °C in presence of protease inhibitor cocktail (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... 800 µL supernatant was incubated with pre-equilibrated MagStrep type 3 XT beads (IBA Life sciences) and rotated for ≥2 hrs at 4°C ...
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bioRxiv - Genetics 2021Quote: ... Strep-tagged Orf8 complexes were eluted from beads in Buffer BXT (IBA Lifesciences; Cat. # 2-1042-025) shaking at 1100 rpm for 30 min.
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bioRxiv - Genetics 2021Quote: ... Strep-tagged Orf8 complexes were eluted from beads in Buffer BXT (IBA Lifesciences; Cat. # 2-1042-025) shaking at 1100 rpm for 30 min.
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bioRxiv - Molecular Biology 2020Quote: ... The elute was then incubated for 1 h at 4 °C with Strep-Tactin beads (IBA Lifesciences) before application to a gravity column ...
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bioRxiv - Cell Biology 2020Quote: ... The clarified supernatant was mixed with 200nM sfGFP-BICDN and 50 µl Streptactin beads (IBA Life Sciences) and rotated end-over-end overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... The soluble supernatant was collected and incubated with pre-equilibrated Strep-Tactin Sepharose beads (IBA Life Sciences) for binding of the Twin-Strep-tagged ULK1 protein ...
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bioRxiv - Biochemistry 2024Quote: ... StrepTactin XT beads (2-5010-025) and biotin (0-1016-002) were purchased from IBA Lifesciences (Germany). Dihydroartemisinin (DHA ...
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bioRxiv - Cell Biology 2024Quote: ... the lysates were cleared by centrifugation and incubated with Strep-Tactin beads (IBA Lifesciences; 2-1206-025) for 2 hours at 4°C ...
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bioRxiv - Biophysics 2023Quote: ... Supernatant was then loaded onto a gravity column containing a Strep-Tactin Sepharose bead slurry (IBA life sciences) for affinity chromatography ...
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bioRxiv - Microbiology 2024Quote: ... Strep-IpaH2.5-3xFLAG-Ub was eluted from the beads in 150 µl Strep-tag elution buffer (Iba LifeSciences). Eluted sample and beads were centrifuged ...
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bioRxiv - Molecular Biology 2021Quote: Strep tagged proteins were then purified by incubating whole cell lysates with MagStrep “type3” XT beads (IBA Life Sciences) for five-six hours at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Eluted fractions were combined and incubated for 1 h at 4°C with Strep-Tactin agarose beads (IBA Lifesciences). Beads were applied to a gravity column ...
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bioRxiv - Microbiology 2022Quote: ... Culture supernatants were harvested 5 days post-transfection and purified with sepharose strep-tactin beads (IBA Life Sciences, Germany) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2023Quote: ... Culture supernatants were harvested 5 days post transfection and purified with sepharose strep-tactin beads (IBA Life Sciences, Germany) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... culture supernatants were harvested five days post-transfection and purified with sepharose strep-tactin beads (IBA Life Sciences, Germany).
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bioRxiv - Molecular Biology 2021Quote: ... 2xStrep tagged SARS-CoV-2 ORFs were immunoprecipitated using 30 μl of Megastrep type 3 XT beads (IBA LifeSciences, Germany), and flag-tagged SARS-CoV-2 ORFs were immunoprecipitated using Anti-DYKDDDDK Magnetic Agarose (Pierce ...
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bioRxiv - Molecular Biology 2021Quote: ... TWIN-Strep tagged proteins were then purified by incubating whole cell lysates from the transiently-transfected cell lines (0.5 ml) with 50 μl of MagStrep “type3” XT beads (IBA Life Sciences) for two-four hours at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble membrane fraction was diluted 2-fold with buffer B and applied to a column packed with Strep-Tactin beads (IBA Lifesciences) by gravity flow (6–10 s/drop ...
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bioRxiv - Microbiology 2022Quote: ... Beads were then washed with buffer B and proteins were eluted using buffer B supplemented with 10 mM desthiobiotin (IBA Lifesciences). Samples were taken at each step of the purification and loaded on SDS-PAGE gels to check the efficiency of affinity purification ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20 μL Strep-Tactin Macroprep beads (IBA lifesciences). Beads were washed three times with lysis buffer ...
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bioRxiv - Neuroscience 2021Quote: ... human SARM1 without mitochondria targeting sequence and with N-terminal tandem StrepTag II was expressed in HEK293T cells expressing NRK1 supplemented with NR (Essuman et al., 2017) and purified with MagStrep (Strep-Tactin) type 3 XT beads suspension (IBA Lifesciences). The purified protein attached on beads was incubated with NAD+ (10 μM for Figure 5 B ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20-30 μl Strep-Tactin Macroprep beads (IBA lifesciences), FLAG or Ni-NTA beads (Sigma) ...
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bioRxiv - Biochemistry 2023Quote: ... and the clarified lysate was then loaded onto a gravity flow column packed with Strep-tactin XT agarose beads (Iba Lifesciences). After binding ...
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bioRxiv - Biochemistry 2023Quote: ... were mixed in PBS in total volume of 50 μL for 1 h at RT and subsequently precipitated with Strep-Tactin beads (Iba Lifesciences). The mixtures were washed twice with PBS ...
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bioRxiv - Biochemistry 2024Quote: ... A pulldown was performed by incubating the RNA/ARID mixture with 1 μl of washed MagStrep “type3” XT beads (IBA Lifesciences, Göttingen) for 1 h at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... A pulldown was performed by incubating the RNA/ZnF mixture with 1 μl of washed MagStrep “type3” XT beads (IBA Lifesciences, Göttingen) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... we added an additional purification step and incubated the Flag-eluted samples with Strep-TactinXT 4Flow beads (IBA lifesciences, 2-5030-010) for 2 hours ...
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bioRxiv - Cancer Biology 2024Quote: ... The Supernatant was than loaded onto a gravity column for affinity chromatography containing a Strep-Tactin Sepharose bead slurry (IBA life sciences). The column was washed with a high salt (1M NaCl ...
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bioRxiv - Microbiology 2024Quote: ... The protein lysates were normalized to 1 mg/ml for their concentration and subjected to affinity precipitation using 15 μl Strep-II tagged beads (IBA Lifesciences GmbH) with a constant agitation at 4 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... for 15 min on ice to block medium biotin content and subsequently loaded on 2 mL Strep-TactinXT4Flow beads (IBA LifeSciences, Göttingen). Beads were washed with 20 mL PBS and protein was eluted in PBS + 50 mM biotin ...
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bioRxiv - Cell Biology 2021Quote: ... The Streptag pulldown was completed in the same manner using 100 μL of MagStrep "type3" XT bead suspension (IBA Lifesciences, 2-4090-002), except elution was achieved by boiling with sample buffer at 95 °C for 2 minutes.