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Citations for IBA Life Sciences :
51 - 100 of 100 citations for SARS CoV 2 Spike Glycoprotein S1 RBD His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... 2.5 mM desthiobiotin (IBA lifesciences, 2-1000-002). The eluted fractions containing highest concentration of the expressed protein were pooled and dialyzed against the storage buffer containing 10mM Tris/HCl ...
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bioRxiv - Genetics 2021Quote: ... streptactin DY-488 (IBA Lifesciences; Cat. # 2-1562-050; 1:500) was added to secondary antibody solution ...
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bioRxiv - Microbiology 2022Quote: ... StrepMAB-Classic (1:500, mouse monoclonal, IBA lifesciences, 2-1507-001), anti-SARS-CoV-2 Spike (1:100 ...
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bioRxiv - Biochemistry 2024Quote: ... and purified on StrepTactin-XT resin (IBA Lifesciences, 2-5010-002) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... and Strep-Tactin Purification Buffer Set (IBA Lifesciences Cat # 2-1002-001) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: 1 mL BioLock biotin blocking solution (cat# 2-0205-050, IBA Lifesciences) was added per 1.5 L of conditioned medium and incubated for 2 h gently stirring at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... The Strep-Tactin Superflow high-capacity resin (IBA lifesciences, 2-1208-002) was used to purify the protein ...
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bioRxiv - Neuroscience 2021Quote: ... Cell lysate was immunoprecipitated with Magstrep Type3 beads (IBA Lifesciences; 2-4090-002) overnight with end/end rocking at 4°C following the protocol provided by the manufacturer ...
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bioRxiv - Molecular Biology 2021Quote: ... and applied to Strep-Tactin affinity chromatography column (IBA Lifesciences, #2-1201-025) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... and a Strep-Tactin Purification Buffer Set (IBA Lifesciences Cat#2-1002-001). The S-2P variants were further purified using a Superose 6 10/300 GL column ...
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bioRxiv - Cell Biology 2023Quote: ... and loaded to a Strep-Tactin Sepharose column (IBA Lifesciences, 2-1201-010). The column was washed with buffer ST200 ...
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bioRxiv - Biochemistry 2020Quote: ... pH 8.0 and loaded into a Streptactin Superflow cartridge (IBA Lifesciences 2-1238-001). Cartridge was washed with 100 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... 50 µl of a 10% slurry of Strep-Tactin Resin (IBA Lifesciences #2-1201-002), which was pre-equilibrated in binding buffer ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was mixed with 2 mL Strep-Tactin XT resin (IBA Lifesciences, Göttingen, Germany). The resin was washed extensively with wash buffer (50 mM Tris ...
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bioRxiv - Cell Biology 2019Quote: ... The lysate was mixed with 2 ml Strep-Tactin XT resin (IBA Lifesciences, Göttingen, Germany). The resin was washed with wash buffer (50 mM Tris ...
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bioRxiv - Molecular Biology 2022Quote: ... or Strep-Tactin®XT 4Flow high capacity resin (IBA life sciences, 2-5030-002). Bound proteins were eluted with 0.2 mg/mL 1xFLAG (DYKDDDK) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the supernatant was incubated with 2ml Strep-Tactin resin (#2-1201-025, IBA Lifesciences) for 30 min at 4°C ...
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bioRxiv - Biochemistry 2023Quote: ... A 1 ml Strep-Tactin XT 4Flow high-capacity cartridge (IBA Lifesciences 2-5027-001) was washed with 14 column volumes of wash buffer (50 mM Hepes pH 7.6 ...
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bioRxiv - Biophysics 2023Quote: ... and the supernatant was incubated with 2ml Strep-Tactin resin (#2−1201-025, IBA Lifesciences) for 30 min at 4°C ...
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bioRxiv - Immunology 2024Quote: ... and recombinant proteins were purified using Strep-Tactin XT resin (IBA Lifesciences, Cat. #2-5010), then buffer exchanged into PBS and concentrated using Amicon Ultracel centrifugal filters (EMD Millipore ...
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bioRxiv - Cancer Biology 2023Quote: ... The extract was incubated with Strep-Tactin XT superfluid resin (2-4010; IBA Lifesciences, Gottingen, Germany) at 4°C for 4 hours ...
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bioRxiv - Biophysics 2021Quote: ... Clarified lysate was placed on a Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in AcrIIA11 Lysis Buffer ...
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bioRxiv - Biochemistry 2021Quote: Labelled and unlabelled DNA oligos to make lacCONS+2 promoter constructs were purchased from IBA Lifesciences (Germany) (Fig ...
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bioRxiv - Genetics 2021Quote: ... Strep-tagged Orf8 complexes were eluted from beads in Buffer BXT (IBA Lifesciences; Cat. # 2-1042-025) shaking at 1100 rpm for 30 min.
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bioRxiv - Genetics 2021Quote: ... Strep-tagged Orf8 complexes were eluted from beads in Buffer BXT (IBA Lifesciences; Cat. # 2-1042-025) shaking at 1100 rpm for 30 min.
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bioRxiv - Microbiology 2022Quote: ... 2xStrep tagged S-protein was stained using StrepMAB-Classic DY-488 conjugate (IBA Lifesciences, 2-1563-050) and FLAG tagged S-protein was stained using anti-FLAG (clone M2 ...
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bioRxiv - Biochemistry 2024Quote: ... StrepTactin XT beads (2-5010-025) and biotin (0-1016-002) were purchased from IBA Lifesciences (Germany). Dihydroartemisinin (DHA ...
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bioRxiv - Microbiology 2024Quote: ... 1mM EDTA and 2 mM biotin) and eluted with 3X volume of BXT buffer (IBA Life Sciences). Samples were run on 12% TGX gels (Biorad ...
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bioRxiv - Systems Biology 2021Quote: ... The supernatant was passed through a column packed with Strep-Tactin® resin (IBA LifeSciences #2-1208-010) and washed with IBA LifeSciences Buffer W (NC0612462 ...
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bioRxiv - Immunology 2021Quote: ... before staining cells with DY-549-labeled strep-tactin (1:100 dilution, IBA lifesciences, Cat #2-1565-050) or Allphycocyanin-labeled streptavidin (1:200 dilution ...
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bioRxiv - Cancer Biology 2019Quote: ... pooled and loaded onto a gravity column containing Strep-Tactin Superflow high capacity resin (#2-1208-025, Iba Lifesciences). Strep-tagged proteins were eluted using 20 mM Tris-HCl pH 8.0 ...
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bioRxiv - Microbiology 2023Quote: ... membranes were incubated with StrepMAB-Classic monoclonal antibody conjugated to HRP (0.05 µg/mL, #2-1509-001, Iba Lifesciences) for 2 h at RT ...
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bioRxiv - Immunology 2021Quote: Purified E1E2 + AR4A-StrepII complexes (1.0 μg ml™1 in TBS with 0.1% DDM) were coated for 2 h at room temperature on 96-well Strep-Tactin XT coated microplates (IBA LifeSciences). Plates were washed twice with TBS/0.05% Tween20 before incubating with serially diluted mAbs and CD81-LEL-hFc (R&D systems ...
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bioRxiv - Microbiology 2023Quote: ... Concentrated proteins were resuspended in buffer W at pH 8 and incubated with BioLock (#2-0205-050, Iba Life sciences) for 15 min at RT to neutralize biotin ...
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bioRxiv - Immunology 2024Quote: ... stabilized histidine-tagged SARS-CoV-2 S protein (Wuhan, XBB.1.5, BA.2.86) was labelled with Strep-Tactin™XT Conjugate DY-649 (IBA Lifesciences) and conjugated to magnetic beads according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... The soluble membrane fraction was diluted 2-fold with buffer B and applied to a column packed with Strep-Tactin beads (IBA Lifesciences) by gravity flow (6–10 s/drop ...
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bioRxiv - Microbiology 2020Quote: Purified StrepII-tagged sE2 monomer (1.0 µg/mL in TBS) was coated for 2 hour at room temperature on 96-well Strep-TactinXT coated microplates (IBA LifeSciences). Plates were washed with TBS twice before incubating with serially diluted mAbs and CD81-LEL-hFc (R& D systems ...
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bioRxiv - Plant Biology 2020Quote: ... They were next incubated in primary antibody solution for 2 hours at room temperature (1:1000 anti-Strep, IBA Life Sciences, 1:10,000 anti-FLAG M2 monoclonal antibody ...
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bioRxiv - Synthetic Biology 2023Quote: ... Gravity flow columns (2 mL) were prepared with 400 μL 50% Strep-Tactin® XT Superflow High Capacity suspension (IBA Lifesciences). Protein purification was performed according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2021Quote: ... Cellular debris was pelleted by ultracentrifugation before placing lysate over a Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Insoluble materials were discarded and clear lysates were added to Strep-Tactin sepharose (400 μL 50% slurry; IBA Lifesciences #2-1201-010) loaded columns (Bio-Rad Laboratories #732-6008 ...
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bioRxiv - Microbiology 2024Quote: ... Bound protein was washed five times with IP buffer and eluted with Strep-Tactin elution buffer (IBA Life Sciences, 2-1000-025). Eluted samples were analyzed by western blot.
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bioRxiv - Microbiology 2024Quote: ... 0.5-1 mg of lysate was incubated overnight with 30 ul of Strep-Tactin Sepharose resin (IBA Life Science, 2-1201-002), rotating at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... we added an additional purification step and incubated the Flag-eluted samples with Strep-TactinXT 4Flow beads (IBA lifesciences, 2-5030-010) for 2 hours ...
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bioRxiv - Cell Biology 2021Quote: ... The Streptag pulldown was completed in the same manner using 100 μL of MagStrep "type3" XT bead suspension (IBA Lifesciences, 2-4090-002), except elution was achieved by boiling with sample buffer at 95 °C for 2 minutes.
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bioRxiv - Immunology 2022Quote: ... in TSM binding buffer at 4°C for 1 h before StrepMAB-Classic Oyster 645 conjugate (0.5 μg/mL, IBA Lifesciences 2-1555-050) was added ...
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bioRxiv - Cell Biology 2022Quote: ... membranes were incubated for 1 h at room temperature in blocking solution with mouse StrepMAB antibody (IBA Lifesciences, 1:750, 2-1507-001) for Strep-tagII::MLC-4 detection or mouse anti-6xHistidine tag antibody (Millipore clone HIS.H8 ...
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bioRxiv - Biochemistry 2024Quote: ... 5 min) and incubated on ice for 30 min with 100 nM scFv and StrepTactin-XT DY647 conjugate (IBA Lifesciences, 2-1568-050) at a 1:200 dilution in 1% BSA in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... with half immunoprecipitated for FLAG-ATF6 with 15 μL G1 anti-DYKDDDK resin and the other half affinity purified for nsp3.1-ST using 15 μL Strep-Tactin XT Superflow High Capacity resin (IBA Life Sciences, 2-4030-002) and rocked at 4°C overnight ...
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bioRxiv - Biophysics 2021Quote: ... The TS-SUMO-SaCas9-sgRNA or TS-SUMO-SaCas9 cell lysate was applied to a 5 mL Strep-Tactin Superflow 50% suspension (IBA Life Sciences, 2-1206-025) gravity column equilibrated in Lysis Buffer containing 200 mM NaCl ...