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Citations for IBA Life Sciences :
51 - 74 of 74 citations for 7 1 3 dioxobutyl amino 3 hydroxynaphthalene 1 sulphonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Strep-Tactin XT resin (1 mL) was prepared following the manufacturer’s instructions for gravity flow purification (Iba Life Sciences). After loading the cleared lysate ...
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bioRxiv - Molecular Biology 2024Quote: ... and eluted with Strep-Tactin elution buffer (100 mM Tris-Cl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 2.5 mM desthiobiotin) (IBA Lifesciences). Elution fractions were analyzed by SDS-PAGE ...
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bioRxiv - Molecular Biology 2024Quote: ... and eluted with Strep-Tactin elution buffer (100 mM Tris-Cl pH 8.0, 150 mM NaCl, 1 mM EDTA, and 2.5 mM desthiobiotin) (IBA Lifesciences). Eluate was concentrated with Amicon Ultra centrifugal filter units (MilliporeSigma) ...
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bioRxiv - Molecular Biology 2023Quote: ... The complex was eluted in five 1 mL fractions using buffer C with added 5 mM desthiobiotin (IBA Lifesciences). After addition of TCEP to 1 mM ...
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bioRxiv - Neuroscience 2021Quote: ... The following primary antibodies were used: StrepMAB-Immo (anti-Twin-Strep-tag®; IBA Lifesciences; 2-1517-001; 1:4000), FUS (1:2000 ...
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bioRxiv - Immunology 2021Quote: Transfected cells were sorted for HDR+ by staining for Strep-Tactin-APC 1:100 (IBA lifesciences, Cat: 6-5010-001) and anti-hIgG AF488 1:100 (Jackson ImmunoResearch ...
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bioRxiv - Molecular Biology 2022Quote: ... The column was washed until baseline and the protein was eluted with PBS containing 1 mM DTT and 2.5 mM desthiobiotin (IBA Lifesciences). Fractions were pooled based on the chromatogram and SDS-PAGE ...
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bioRxiv - Microbiology 2023Quote: ... The supernatant was subjected to affinity chromatography using 1 mL buffer equilibrated Strep-Tactin XT 4Flow resin (IBA Lifesciences-GmbH) and flow through was collected ...
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bioRxiv - Biochemistry 2020Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated either with anti-strep tag antibody (IBA Lifesciences) or anti-SARS-COV-2 polyclone antibody (Sino Biological Inc. ...
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bioRxiv - Plant Biology 2020Quote: ... They were next incubated in primary antibody solution for 2 hours at room temperature (1:1000 anti-Strep, IBA Life Sciences, 1:10,000 anti-FLAG M2 monoclonal antibody ...
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bioRxiv - Biochemistry 2021Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20 μL Strep-Tactin Macroprep beads (IBA lifesciences). Beads were washed three times with lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... and 1 ml of soluble protein fractions were incubated for 1 h at 4 °C with 20-30 μl Strep-Tactin Macroprep beads (IBA lifesciences), FLAG or Ni-NTA beads (Sigma) ...
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bioRxiv - Cancer Biology 2022Quote: ... PBMCs from HLA-A*0201 Donors were either stimulated with 1 µg/mL CMV pp65 antigen peptide NLVPMVATV (HLA-A*0201) (IBA Lifesciences) for 1 h or not ...
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bioRxiv - Molecular Biology 2022Quote: ... Cleaved-off alkaline phosphatase and TEV protease were removed by re-purifying EXT1-EXT2 using a second HisTrap HP purification step and by passing protein sample through 1 mL Strep-Tactin XT resin (IBA Lifesciences). Buffer was exchanged by several rounds of concentration and dilution with buffer B (20mM HEPES pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: ... were mixed in PBS in total volume of 50 μL for 1 h at RT and subsequently precipitated with Strep-Tactin beads (Iba Lifesciences). The mixtures were washed twice with PBS ...
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bioRxiv - Biochemistry 2023Quote: Expression of each mutant in the membrane samples was evaluated through western blotting using 10 ng mL-1 Strep-Tactin conjugated with horseradish peroxidase (IBA Lifesciences) and using SYPRO Ruby (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2023Quote: ... Supernatants were incubated with end-over-end rotation for 1 hour at 4°C with Strep-Tactin Superflow high-capacity resin (IBA Lifesciences) equilibrated with gel-filtration buffer containing 20 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... were formed as described previously (Sidorenkov et al., 1998) using scaffold 1 (Table 6) and the RNA sequence: AUCGAGAGG (IBA life sciences). Briefly ...
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bioRxiv - Synthetic Biology 2022Quote: ... The solution was then centrifuged 3 times at 20,000 × g at 4 °C for 10 min periods and the supernatant was used for purification using fast protein liquid chromatography (FPLC) and 1 mL Strep-Tactin Superflow high capacity column (IBA Lifesciences, Germany). After equilibration with 5 column volume (CV ...
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bioRxiv - Biochemistry 2024Quote: ... A pulldown was performed by incubating the RNA/ZnF mixture with 1 μl of washed MagStrep “type3” XT beads (IBA Lifesciences, Göttingen) for 1 h at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... 0.5-1 mg of lysate was incubated overnight with 30 ul of Strep-Tactin Sepharose resin (IBA Life Science, 2-1201-002), rotating at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... A pulldown was performed by incubating the RNA/ARID mixture with 1 μl of washed MagStrep “type3” XT beads (IBA Lifesciences, Göttingen) for 1 h at 4°C ...
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bioRxiv - Immunology 2021Quote: SEC purified HIV-1 Env-Soc trimers were tested for antigenicity using ELISA involving Microplates pre-coated with Strep-Tactin (IBA Life Sciences GmbH). Microplates were coated with 1 µg/ml SEC-purified gp140-Soc trimers in a volume of 100 µl/well of coating buffer (25 mM Tris-HCl ...
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bioRxiv - Immunology 2022Quote: ... in TSM binding buffer at 4°C for 1 h before StrepMAB-Classic Oyster 645 conjugate (0.5 μg/mL, IBA Lifesciences 2-1555-050) was added ...